Mallow Carotenoids Determined by High-performance Liquid Chromatography
Jafar Muhammad El-Qudah,
Mallow (Corchorus olitorius) is a green vegetable, which is widely consumed either fresh or dry by Middle East population. This study was carried out to determine the contents of major carotenoids quantitatively in mallow, by using a High Performance Liquid Chromatography (HPLC) equipped with a Bischoff C30 column. Neoxanthin and violaxanthin in mallow were characterized by Liquid Chromatography-Mass Spectrometry (LC-MS) with Atmospheric Pressure Chemical Ionization (APCI) as an ionization interphase. The total carotenoid content of mallow was estimated as 16.9 mg/100 g fresh weight. In which, major carotenoids in mallow are: lutein (5.3 mg/100 g), anhydrolutein (4.4 mg/100 g), β-carotene (3.7 mg/100 g), cis-anhydrolutein (1.0 mg/100 g), neoxanthin (1.6 mg/100 g), violaxanthin (0.3 mg/100 g), 9-cis-β- carotene (0.4 mg/100 g) and 13-cis-β-carotene (0.09 mg/100 g). Therefore, mallow is a rich source of carotenoids. As a popular vegetable consumed by the Middle East and surrounding populations, our finding of this study confirmed that mallow is an important source for carotenoids including provitamin A carotenoids.
August 02, 2011; Accepted: November 19, 2011;
Published: January 02, 2012
Mallow (Corchorus olitorius) is a green plant vegetable. The Latin name
for this plant is Corchorus Olitorious. Its family name is Tiliaceae, and its
Botanical references is 200 (Okusanya, 1979; Rahman,
1978; Verma and Arora, 1978). Mallow is grown in
Mediterranean area, and popularly consumed by the Middle East population including
Jordan, Syria, Egypt and many other countries in the Middle East region. It
can make a mucilaginous, nutritious green soup with its crushed green leaves.
The Arabic name of this dish is molokheya, and it is transliterated to several
forms as, Moulokheyya, Mulukheyya, Mooookhieh, Mouloukhia, Mloukhia, Molohia,
Molokhiya, Molukhyia, Mulukhia, Mulukhiya, Melokiyah, Meloukhia and other variations.
Some people believe that Molokheya is originated in Egypt during the time of
Pharaohs. It is also known as Egyptian Spinach. Others suggest that it was prepared
first by ancient Jews. It is said that the original name for molokheya is Mulukeyya,
which means of the kings, then the public turned the K to the present KH (Chen
and Saad, 1981).
It is generally accepted that the significance of vegetable consumption can
play an important role in maintaining health and reducing the risk of illness
(Kalt, 2005). Further, consumption of vegetables and
fruits high in α-carotene, β-carotene and lycopene such as carrots,
spinach and tomatoes is associated with lower incidence of certain types of
cancer, including lung, skin and digestive tract cancers (Franceschi
et al., 1994; Mayne et al., 1994;
Murakoshi et al., 1992). This is due to the facts
that carotenoids are considered to be antioxidants in biological tissue as well
as in food systems (Anguelova and Warthesen, 2000).
Dietary carotenoids, or food rich in these colorful pigments, are considered
to be beneficial in the prevention of a variety of major disease, including
certain cancers and eye diseases (Krinsky and Johnson, 2005).
Of all the carotenes, β-carotene is the most important one, because not
only it can convert to Vitamin A in humans to provide Vitamin A nutrient but
also it can function to prevent photosensitization and formation of skin tumors
and potentially to increase immune response (Rao and Rao,
2007). In addition, it was proved that carotenoids have a favorable effect
on influencing the cell cycle progression of the fibroblasts (Stivala
et al., 1996).
In the Middle East, traditional vegetables, including mallow, are important
source of nutrients and vitamins for many persons in the community. In Jordan,
there are few researches on carotenoid pigments from natural products in human
diets (Takrury, 1986; El-Qudah, 1997,
The aims of this study are to determine the carotenoid contents in mallow, that is, to identify and quantitatively determine mallow carotenoids by using HPLC and LC-MS.
MATERIALS AND METHODS
Sample preparation: Due to the light sensitivity of carotenoids, the
analysis was performed under red lights. The extraction of the vegetable, using
a modified procedure from Riso and Porrini (1997), was
performed by incubating 500 mg of the pureed vegetable together with 10 mL methanol
for 1 h in a shaking incubator at 120 rpm. Afterwards, the mixture was homogenized
for 30 sec in an ice bath and the probe washed with methanol. The mixture was
centrifuged at 3000 rpm for 5 min. The methanol layer was transferred into a
50 mL volumetric flask and the extraction repeated four times with 10 mL of
Tetrahydrofuran (THF), followed by vortexing and centrifugation. The THF layers
were combined with the methanol layer and the volume brought up to 50 mL. One
milliliter of the extract was taken, dried under nitrogen and re-suspended in
1 mL of ethanol to be used for further analysis.
Reagent, chemicals and source of sample: High liquid chromatography grade ethanol, Methyl-Tert-Butyl Ether (MTBE) and ammonium acetate (analytical grade) were purchased from Sigma Company (St Louis, MO, USA). Milli Q water was obtained from Water Purification Systems and used for all procedures using water. A Bischoff C30 column (3.0 μm, 3.0x150 mm) was purchased from Mac-mod Analytical Company (Chadds Ford, PA, USA). Lutein and zeaxanthin standards were gifts from DSM Nutritional Products (Basel, Switzerland) and were dissolved in ethanol for HPLC analysis. Mallow was purchased at a vegetable market from Boston, MA, USA.
Chromatography, HPLC and LC/APCI-MS analysis: High Performance Liquid
Chromatography (HPLC) analysis was performed using the Waters Alliance 2695
(Milford, MA, USA) equipped with a Bischoff C30 column. Mobile phase A was composed
of methanol, Methyl terbutyl ether (MTBE), water and NH4Ac with the
following ratios according to Li et al. (2007):
60:33:7 (v/v/v) (1.5% NH4Ac in water). Mobile phase B contained (8%)
methanol, (90%) MTBE, (2.0%) water and (1.0%) NH4Ac in water.
The gradient procedure was as follows: (1) a 100% mobile phase A for 3 min; (2) a 15 min linear gradient to 80% mobile phase A; (3) a 17 min linear gradient to 55% mobile phase B; (4) a 15 min linear gradient to 95% mobile phase B and (5) a 2 min gradient back to 100% mobile phase A. Once the gradient was completed, the HPLC mobile phase was kept at 100% mobile phase A and the column was re-equilibrated for 15 min. The flow was 0.4 mL min-1 and the injection volume was 20 μL. Lutein, zeaxanthin and their isomers were measured at 445 nm.
To determine the mass of the two unidentified peaks, the LC-MS method with a positive ion mode was used. The LC was an Agilent 1100 and the mass spectrometers was a Bruker Esquire LC equipped with an Atmospheric Pressure Chemical Ionization interface (APCI). The extracted mallow samples were re-suspended in ethanol and injected into the on-line LC/APCI-MS. The mass spectrometer was set to scan the range of 530 - 630 Dalton and a temperature of the ionization chamber was set at 300°C. The voltage of the corona needle was optimized, resulting in a current of 4 to 8A. The drying and carrier gas was nitrogen at a temperature of 300°C.
Dietary intake: A cross-sectional dietary survey was carried out in
a random sample of the adult population living in Amman, Jordan . Dietary intake
was assessed by means of a Food-Frequency Questionnaire (FFQ), which was designed
to estimate food intake over the past year (Nasreddine et
al., 2006). A self- reported questionnaire was administrated to 200
participants (100 males and 100 females), ranging in age from 18-70 years. The
individual was asked to estimate the number of times per day, week, month or
year he/she consumed this particular food product and the amount usually eaten
per food item by making comparisons with the specified reference portion. The
dietary intake of mallow was determined by using FFQ and data are expressed
as serves per person per week. A serving of vegetable was defined as half a
cup of cooked Mallow.
Table 1 shows dietary intake of cooked mallow by a sample
of 200 adults residents in Jordan (100 men and 100 women) age 18-70 year.
|| Dietary intake of mallow by men and women in Jordan
|Data are expressed as serving/ person/ week
|| Carotenoid contents of Mallow (μg/100 g)a
|aMean of eight replications±SD
||Chromatographic profiles of mallow analyzed by HPLC. 1: Neoxanthin;
2: Violaxanthin; 3: Lutein; 4: Anhydrolutein; 5: Cis-anhydrolutein; 6: Chlorophyll;
7: 13-cis-β-carotene; 8: total β-carotene; 9: 9-cis-β-carotene
|| Mass spectrum of mallow neoxanthin analyzed using APCI-LC/MS
The average consumption is 1.27 cup per person per week. This indicates that
its consumption is relatively high among Jordanian and it is well accepted meal.
Mallow is usually consumed with rice and chicken, which increase the nutritive
value of the whole meal.
Using standards, relative retention times and spectral characteristics carotenoids
in mallow were identified. Figure 1 shows chromatographic
profiles of mallow. Lutein, anhydrolutein and β-carotene were the major
identified peaks. For further identification, neoxanthin and violaxanthin (Fig.
2, 3) were characterized by LC/APCI-MS in a positive mode.
The results for lutein, anhydrolutein (Budowski et al.,
1963), cis-anhydrolutein, neoxanthin, violaxanthin, β-carotene, 13-cis-β-carotene
and 9-cis-β-carotene content are presented in Table 2.
The results presented in the table corresponding to the mean value of 8 replicates.
The standard deviation for eight different samples is also included in the Table
2. The total carotenoids in mallow were estimated as a sum of lutein, anhydrolutein,
cis-anhydrolutein, neoxanthin, violaxanthin, β-carotene, 13-cis-β-carotene
and 9-cis-β-carotene content per unit weight. Lutein was found in highest
amounts (5333±9 μg/ 100 g), representing about 31.4% of the total
carotenoids content, followed by anhydrolutein (4417±6 μg/100 g)
and β-carotene (3708±7 μg/100 g).
In this study we have identified appreciable levels of lutein and β-carotene
in mallow, which is a green leafy vegetable commonly consumed as part of a Mediterranean
|| Mass spectrum of mallow violaxanthin analyzed using APCI-LC/MS
Various studies have shown a significant correlation between habitual vegetable
and fruit intake and plasma carotenoid concentrations (Martini
et al., 1995; El-Sohemy et al., 2002;
Agudo et al., 2002; Jansen
et al., 2004).
Although there is no data on carotenoids content of mallow in the USDA National
Nutrient database , the food composition tables for use in the Middle East show
that the content of 100 g edible portion of mallow is 1260 retinol equivalent
based on a conversion factor of 6 μg β-carotene or 12 μg other
provitamin A carotenoid pigments to 1 μg retinol (Pellett
and Shadarvian, 1970). FAO database indicates that the content of 100 g
of raw leaves of mallow contain 6400 μg β-carotene (FAOUN,
1982). A recent study conducted in Tanzania, shows that the β-carotene
content of Jute mallow in Singida distinct and Muheza district were 1.91 and
6.31 mg/100 g, respectively (Weinberger and Msuya, 2004).
The total carotenoid found in mallow by this study was about 16.9 mg/100 g
and in which, β-carotene was about 3.7 mg/100 g. These values were higher
than or approximately equal to those found in other commonly consumed vegetables.
The β-carotene content in mallow is approximately two third of the value
of spinach reported in the USDA database (U.S. Department
of Agriculture, Agricultural Research Service, 2006). Similar contents of
β-carotene are also seen in Korean database (National
Rural Living Science Institute, 2001) and the Austrian database (Murkovic
et al., 2000).
β-carotene is the most important provitamin A, having about twice as much
activity as α-carotene. Lutein is the carotenoid implicated in the reduced
risk of cataract and muscular degeneration (Moeller et
al., 2000), together with zeaxanthin. It has also been reported to be
inversely associated with colon cancer in both men and women (Slattery
et al., 2000). Although possible health benefits of neoxanthin, violaxanthin,
lactucaxanthin and other carotenoids have not been shown, their determination
is important for future studies, as they have similar structures to those of
carotenoids considered important to human health.
Vegetable mallow has lutein, anhydrolutein and β-carotene as principal carotenoids. The results of this study will provide basic distary carotenoids contents to update the Middle East food nutrition database. Thus, the information of dietary mallow carotenoids will help to better estimate dietary intakes and adequacies of vitamin A nutrition from food consumption surveys conducted in the region.
The authors wish to thank USAID for their fund and Jean Mayer USDA-HNRCA, Tufts University for a fellowship given to the first author.
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