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Research Article

Role of Biological Control on Some Physiological Aspects of Zea mays Infected by Rhizoctonia solani

Faten A. El-Daly and Nahed Haikal
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The results revealed that treatment with either Trichoderma harzianum or Bacillus subtilis by soil inoculation or grain coating significantly increased the percentage of healthy seedlings as well as the length, fresh and dry weight of seedlings. Photosynthetic pigments content of the leaves significantly increased in absence of Rhizoctonia solani alone. The same almost applied to soluble sugar content, amino acid content or total nitrogen of the seedlings, though less apparent or insignificant when the grains were treated with B. subtilis before growing in soil treated with 3% R. solani. R. solani lowered the test elemental content of Zea mays seedlings, while the reverse was most prominent by sowing the grains in soil amended with R. solani and T. harzianum. The results also revealed that infestation by Rhizoctonia solani significantly lowered the length of the ears and weight of 100 grains. In the mean time the weight of 100 grains significantly dropped; a response that was hardly, if at all affected by implying R. solani with Bacillus subtilis or T. harzianum. The presence of the three microorganisms increased the fresh weight of the ears but the total count or weight of the grains was lowered. The presence of R. solani in soil lowered the lipid, total carbohydrates and protein content of corn flour. Meanwhile using the biological control agents T. harzianum or B. subtilis or both initiated the increase of these components.

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  How to cite this article:

Faten A. El-Daly and Nahed Haikal , 2006. Role of Biological Control on Some Physiological Aspects of Zea mays Infected by Rhizoctonia solani. Journal of Applied Sciences, 6: 2794-2798.

DOI: 10.3923/jas.2006.2794.2798



1:  Bae, Y.S., S.S. Iang, C.S. Park and H.K. Kim, 1995. In vitro and green house evaluation of cucumber growth enhanced by Rhizosphere microorganisms. Korean J. Plant Pathol., 11: 292-297.

2:  Baker, R., 1986. Biological control: An overview. Can. J. Plant Pathol., 8: 218-221.

3:  Bausch, P., W. Schuster and E. Schlosser, 1982. Susceptibility of maize seedlings to root and stalk rots of Zea mays. Angew. Bot. Gottingen., 56: 29-56.

4:  Cooper, G.R. and V. McDaniell, 1970. Standard Methods for Clinical Chemistry. Academic Press, New York and London, pp: 6:159

5:  De Freitas, J.R. and J.J. Germida, 1991. Pseudomonas cepacia and Pseudomonas putida as winter wheat inoculants for biocontrol of Rhizoctonia solani. Can. J. Microbiol., 37: 780-784.
CrossRef  |  Direct Link  |  

6:  Dertinger, U., U. Schaz and E. Schulze, 2003. Age-dependence of the antioxiodative system in tobacco with enhanced glutathione-induced production of cytokinins. Physiol. Plant, 119: 19-29.

7:  Gasque, C.E., 1982. Comparison of cytokinine activities of 9-substituted N-benzyladenines in the C. sativus and Amaranthus bioassays. Phytochemistry, 21: 1501-1507.

8:  Harman, G.E., C.R. Howell, A. Viterbo, I. Chet and M. Lorito, 2004. Trichoderma species-opportunistic, avirulent plant symbionts. Nat. Rev. Microbiol., 2: 43-56.
CrossRef  |  PubMed  |  Direct Link  |  

9:  Howell, C.R., L.E. Hanson, R.D. Stipanovic and L.S. Puckhaber, 2000. Induction of terpenoid synthesis in cotton roots and control of Rhizoctonia solani by seed treatment with Trichoderma virens. Phytopathology, 90: 248-252.
CrossRef  |  Direct Link  |  

10:  Hynes, R.K., J. Hill, M.S. Reedy and G. Lazarovits, 1994. Phytoalexin production by wounded white bean (Phaseolus vulgaris) cotyledons and hypocotyls in response to inoculation with rhizobacteria. Can. J. Microbiol., 40: 548-554.
Direct Link  |  

11:  Jordi, W., A. Schapendonk, E. Davelaar, G.M. Stoopen and C.S. Pot et al., 2000. Increased cytokinine levels in transgenic PSAG12-IPT tobacco plants have large direct and indirect effects on leaf senescence, photosynthesis and N partitioning. Plant Cell Environ., 23: 279-289.
Direct Link  |  

12:  Levenfors, J.J., R. Hedman, C. Thaning, B. Gerhardson and C.J. Welch, 2004. Broad-spectrum antifungal metabolites produced by the soil bacterium Serratia plymuthica A 153. Soil Biol. Biochem., 36: 677-685.
CrossRef  |  Direct Link  |  

13:  Lichtenthaler, H.K., 1987. Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes. Methods Enzymol., 148: 350-382.
CrossRef  |  Direct Link  |  

14:  Madhosingh, C., 1995. Relative wilt-inducing capacity of the culture filtrates of isolates of Fusarium oxysporum f. sp. radicis-lycopersici, the tomato crown and root rot pathogen. J. Phytopathol., 143: 193-198.
Direct Link  |  

15:  Marino, G. and G. Bertazza, 1990. Micropropagation of Actinidia deliciosa cvs. Hayward and Tomuri. Sci. Hortic., 45: 65-74.
Direct Link  |  

16:  McGee, D.C., 1988. Maize Diseases: A Reference Source for Seed Technologist. 3rd Edn., APS Press, USA

17:  Mutting, D. and E. Kaiser, 1963. Determination of amino-nitrogen. Hoppe Seyler's Z. Physiol. Chem., 332: 276-276.

18:  Naguib, M.I., 1969. On the colorimetry of nitrogen components of plant tissue. Bull. Fac. Sci. Cairo Univ., 43: 1-5.

19:  Oller, E.M.M., J. Chekowski and H.H. Geiger, 1999. Species-specific PCR assays for the fungal pathogens F. moniliforme and F. subglutinans and their application to diagnose maize ear rot disease. J. Phytopathol., 147: 497-508.
Direct Link  |  

20:  Priyatmojo, A., R. Yamauchi, D.E. Carling, K. Kageyama and M. Hyakumachi, 2002. Differentiation of three varieties of Rhizoctonia circinata: Var. circinata, var oryzae and var. zeae on the basis of cellular fatty acid composition. J. Phytopathol., 150: 1-5.
Direct Link  |  

21:  Ran, L.X., C.Y. Liu, G.J. Wu, L.C. van Loon and P.A.H.M. Bakker, 2005. Suppression of bacterial wilt in Eucalyptus uophylla by fluorescent Pseudomonas sp. in China. Biol. Cont., 32: 111-120.
CrossRef  |  

22:  Roberts, D.P., S.M. Lohrke, S.L.F. Meyer, J.S. Buyer and J.H. Bowers et al., 2005. Biocontrol agents applied individually and in combination for suppression of soilborne diseases of cucumber. Crop Prot., 24: 141-155.
CrossRef  |  

23:  Seyer, P., D. Marly, A.M. Lescure and C. Peaud-Lenoel, 1975. Effect of cytokinine on chloroplast cyclic differentiation in cultured tobacco cells. Cell Differen., 4: 187-197.

24:  Sidorov, A.A., 1990. Evaluation of adaptability and stability in barley varieties following infection with root rots. Selelctsiya-l-Semenovodstvo Moskva, 6: 13-15.

25:  Sivan, A., Y. Elad and I. Chet, 1984. Biological control effects of new isolate of Trichoderma harzianum on P. aphanidermaturn. Phytopathology, 74: 498-501.

26:  Stewart, E.A., 1972. Chemical Analysis of Ecological Materials. Blackwell Scientific, London

27:  Straub, V. and H.K. Lichtenthaler, 1973. The effect of gibberellic acid (GA3) and kinetin on the formation of photosynthetic pigments, lipoquinones and anthocyanins in Raphanus seedlings. Ziet. Planzen Physiol., 70: 308-308.

28:  Troshina, N.B. and A.M. Jamaleen, 1991. The effect of Baytan on protein and lipid content in wheat plants and on parasitizing fungi causal agent of root rots. Fiziologiya-I-Biolchimiya Kul'turnkh Rastenii, 23: 402-406.

29:  Vazquez, C., F. Reyes and M.J. Martinez, 1993. Comparative studies of pectic activities from different, formae speciales Fusarium oxosporurm. Applied Microbiol., 16: 210-213.

30:  Wen, Z., W. Liao and S. Chen, 2005. Production of cellulase by Trichoderma reesei from dairy manure. Bioresour. Technol., 96: 491-499.
CrossRef  |  Direct Link  |  

31:  Wilderm, G.B., R.D. Tinline and R.B. McNamara, 1992. Assessment of yield loss caused by common root rot in wheat cultivar in Queensland. Aust. J. Agric. Res., 43: 45-58.
CrossRef  |  

32:  Xiong, D.H., F.H. Xu, P.Y. Liu, H. Shen and J.R. Long et al., 2005. Vitamin D receptor gene polymorphisms are linked to and associated with adult height. J. Med. Genet., 42: 228-234.
CrossRef  |  

33:  Yedidia, I., A.K. Srivastva, Y. Kapulnik and I. Chet, 2001. Effect of Trichoderma harzianum on microelement concentrations and increased growth of cucumber plants. Plant Soil, 235: 235-242.
Direct Link  |  

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