Subscribe Now Subscribe Today
Research Article

Chlorophyll Fluorescence in the Leaves of Soybean Affected by High Temperature Stress

Mohammad Ibrahim Keerio , Saghir Ahmed Sheikh , Shanila Yasmeen Chang and Samiullah K. Agha
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

The chlorophyll fluorescence has been widely used to study the activity of the photosynthetic machinery. The first trifoliate leaf was used to study the effects of high temperature on the leaves using chlorophyll fluorescence method (Fv/Fm ratio). No effects were found on the ratio when leaves were stressed for 2 to 4 h at 35° C. After 4 h stress at 40°C, however, there was an approximately 50% decrease in the Fv/Fm ratio. The Fv/Fm ratio was more severely affected by heat stress at 42.5° C, but effects were greater in cultivars Mago-80 and Bragg. At 45°C there were approximately 80% decreases in the Fv/Fm ratios in all cultivars.

Related Articles in ASCI
Search in Google Scholar
View Citation
Report Citation

  How to cite this article:

Mohammad Ibrahim Keerio , Saghir Ahmed Sheikh , Shanila Yasmeen Chang and Samiullah K. Agha , 2001. Chlorophyll Fluorescence in the Leaves of Soybean Affected by High Temperature Stress. Journal of Applied Sciences, 1: 522-525.

DOI: 10.3923/jas.2001.522.525



1:  Adams, W.W., B. Demmig-Adams, K. Winter and U. Schreiber, 1990. The ratio of variable to maximum chlorophyll fluorescence from photosystem II. Measured in leaves at ambient temperature and at 77K, as an indicator of the photon yield of photosynthesis. Planta, 180: 166-174.
CrossRef  |  Direct Link  |  

2:  Aoki, S., 1990. Measurement of heat sensitivity in cucumber leaves by chlorophyll fluoresence method. Trop. Agric. Res. Ser., 23: 239-247.

3:  Armond, P.A., U. Shreiber and O. Bjorkman, 1978. Photosynthetic acclimation to temperature in the desert shrub, Lorrea divaricata. II. Light-harvesting efficiency and electron transport. Plant Physiol., 61: 411-415.

4:  Bilger, W., U. Schreiber and O.L. Lange, 1987. Chlorophyll Fluorescence as Indicator of Heat Induced Limitation of Photosynthesis in Arbutus unedo L. In: Plant Responses to Stresses, Tenhunen, J.D., F.M. Catarino, O.L. Lange and W.C. Oechel (Eds.). Springer Verlag, Berlin, pp: 391-399

5:  Bjorkman, O, H.A. Mooney and J. Ehleringer, 1975. Comparison of photosynthetic characteristics of intact plants. Carnegie Inst., 74: 743-748.

6:  Christiansen, M.N., 1978. Physiology of Plant Tolerance to Temperature Extremes. In: Crop Tolerance of Suboptimal Land Conditions, Jung, G.A. (Eds.). American Society of Agronomy, Madison, WI., USA., pp: 173-191

7:  Greaves, J.A. and J.M. Wilson, 1987. Assessment of the frost sensitivity of wild and cultivated potato species by chlorophyll fluorescence analysis. J. Potato Res., 30: 381-395.
CrossRef  |  Direct Link  |  

8:  Havaux, M., H. Greppin and R.J. Strasser, 1991. Functioning of photosystems I and II in pea leaves exposed to heat stress in the presence or absence of light. Planta, 186: 88-98.
CrossRef  |  Direct Link  |  

9:  Kislyuk, I.M., 1979. Protecting and injurious effects of light on photosynthetlc apparatus during and after heat treatment on leaves. Photosynthetica, 13: 386-391.

10:  Krause, G.H. and E. Weis, 1984. Chlorophyll fluorescence as a tool in plant physiology II. Interpretation of fluorescence signals. J. Photosynthesis Res., 5: 139-157.
CrossRef  |  Direct Link  |  

11:  Larcher, W., J. Wagner and A. Thammathaworn, 1990. Effect of superimposed temperature stress on in vivo chlorophyll fluorescence of Vigna unguiculata under saline stress. J. Plant Physiol., 136: 92-102.
Direct Link  |  

12:  Larcher, W., 1980. Physiological Plant Ecology. 2nd Edn., Springer Verlag, Berlin, Hendelberg, pp: 303

13:  Moffatt, J.M., R.G. Sears and G.M. PauJsen, 1990. Wheat high temperature tolerance during reproductive growth: I. Evaluation by chlorophyll fluorescence. Crop Sci., 30: 881-885.
Direct Link  |  

14:  Neuner, G. and W. Larcher, 1990. Determination of differences in chilling susceptibility of two soybean varieties by means of in vivo chlorophyll fluorescence measurements. J. Agron. Crop Sci., 164: 73-80.

15:  Oquist, G. and R. Wass, 1988. A portable microprocessor operated instrument for measuring chlorophyll fluorescence kinetics in stress physiology. Physiol. Plant., 73: 211-217.
CrossRef  |  Direct Link  |  

16:  Potvin, C., 1985. Effect of leaf detachment on chlorophyll fluorescence during chilling experiments. Plant Physiol., 78: 883-886.
Direct Link  |  

17:  Sethar, M.A., 1993. A physiological study of heat stress in cotton seedlings. Ph.D. Thesis, University of Wales, Aberystwyth, UK.

18:  Simillie, R.M. and G.C. Gibbons, 1981. Heat tolerance and heat hardening in crop plants measured by chlorophyll fluorescence. Carlsberg Res. Commun., 46: 309-403.
CrossRef  |  Direct Link  |  

19:  Simillie, R.M., 1979. The Useful Chloroplast: A New Approach for Investigating Chilling Stress in Plants. In: Low Temperature Stress in Crop Plants: The Role of Membranes, Lyons, J.M., D. Graham and J.K. Raison (Eds.). Academic Press, London, UK

20:  Somersalo, S. and G.H. Krause, 1989. Photoinhibition at chilling temperature. Plant, 177: 409-416.
CrossRef  |  Direct Link  |  

21:  Somersalo, S. and G.H. Krause, 1990. Photoinhibition at chilling temperatures and effects of freezing stress on cold acclimated spinach leaves in the field: A fluorescence study. Physiol. Plant., 79: 617-622.
CrossRef  |  Direct Link  |  

22:  Wilson, J.M., 1976. The mechanism of chill-and drought-hardening of phaseolus vulgaris leaves. New Phytol., 76: 257-270.
Direct Link  |  

23:  Yucel, M., J.J. Burke and H.T. Nguyen, 1992. Inhibition and recovery of photosystem II following exposure of wheat to heat shock. Environ. Exp. Bot., 32: 125-135.
Direct Link  |  

©  2021 Science Alert. All Rights Reserved