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Phylogenetic Relationships of Two Earth Tiger Tarantulas, Haplopelma lividum and H. longipes (Araneae, Theraphosidae), within the Infraorder Mygalomorph Using 28S Ribosomal DNA Sequences



Arin Ngamniyom, Manaporn Manaboon, Busaba Panyarachun and Udomsri Showpittapornchai
 
ABSTRACT

Haplopelma lividum and H. longipes (Araneae: Mygalomorphae: Theraphosidae) are tarantulas that are distributed throughout Southeast Asia and are important carnivorous predators in ecological systems. The present study aimed to examine the phylogenetic relationships between Mygalomorph spiders using 28S ribosomal DNA sequences. The molecular results supported the placement of both species within a common theraphosid taxon. However, when considering relationships between Haplopelma spp. and related genera, H. schmidti, H. lividum and H. longipes were not monophyletic, suggesting that molecular data are incongruent with phylogenies based on morphological characteristics. These results provide molecular data to help elucidate the phylogenetic relationships between theraphosid tarantulas.

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Arin Ngamniyom, Manaporn Manaboon, Busaba Panyarachun and Udomsri Showpittapornchai, 2014. Phylogenetic Relationships of Two Earth Tiger Tarantulas, Haplopelma lividum and H. longipes (Araneae, Theraphosidae), within the Infraorder Mygalomorph Using 28S Ribosomal DNA Sequences. International Journal of Zoological Research, 10: 15-19.

DOI: 10.3923/ijzr.2014.15.19

URL: https://scialert.net/abstract/?doi=ijzr.2014.15.19
 
Received: August 08, 2013; Accepted: October 30, 2013; Published: April 16, 2014

INTRODUCTION

Theraphosid tarantulas (infraorder Mygalomorphae) are known to be important predators in ecological webs, are kept commercially as pets and are used as research tools in the fields of toxicology, pharmacology, systematic biology and molecular phylogeny (Escoubas and Rash, 2004; Tang et al., 2010; Bertani, 2012). The Earth tiger tarantula is a mid-to large-size venomous spider in the subfamily Ornithoctoninae, belonging to the family of Theraphosidae (Zhu and Zhang, 2008). The Haplopelma tarantula is a genus in Ornithoctoninae and includes, for example, H. albostriatum, H. hainanum, H. schmidti, H. lividum and H. longipes (Simon, 1892). Haplopelma are widely distributed within the tropical environments of South China and Southeast Asia, inhabiting underground burrows and hilly areas protected by flat sheet webs (Pan and Yu, 2010).

H. lividum (Smith, 1996) (which are commonly called cobalt blue tarantulas, are mainly found in Myanmar and Thailand and H. longipes (Von Wirth and Striffler, 2005) are distributed throughout Thailand and Cambodia. However, few studies have been reported on these species. Takaoka et al. (2001) described two case studies of finger bites from H. lividum which appeared relatively harmless compared to bites from other tarantulas. Moore et al. (2009) characterized the crude venom of H. lividum, detecting glutamic acid, histamine, adenosine and polyamine spermine. Von Wirth and Striffler (2005) also described systematic identification of H. longipes using morphological characteristics of the whole body. Within the genus Haplopelma, 28S ribosomal RNA (rRNA) sequence data have only been reported for H. schmidti, placing it within the phylum Chelicerata (Arabi et al., 2012). Phylogenetic relationships and rRNA sequence data for H. lividum and H. longipes have not been evaluated.

Therefore, the present study aimed to examine the phylogenetic tree of two tarantulas (H. lividum and H. longipes, Fig. 1b, respectively) in relation to other species of the suborder Mygalomorphae, based on 28S rRNA gene sequences. This study contributes to the understanding of the evolutionary relationships among theraphosid spiders.

Fig. 1(a-c):
(a) A ventral views of adult H. lividum, (b) H. longipes and (c) Inhabiting a ground of natural environment. A map showed the six localities of Kanchanaburi Province for tarantula collections. Black circle indicates the localities which observed for H. lividum and white circles for H. longipes. Black and white circles indicate the localities which found both two tarantulas

MATERIALS AND METHODS

Tarantula sampling: Adult H. lividum and H. longipes (Fig. 1a-b) inhabiting ground burrows were caught from six localities in Kanchanaburi Province, Thailand (15°9'21"N 98°27'13"E, 14°44'45"N 98°37'30"E, 14°35'57"N 99°6'50"E, 14°6'56"N 99°8'40"E, 14°0'12"N 99°33'0"E and 13°51'13"N 99°24'38"E) (Fig. 1c). The species of those tarantulas was identified according to the criteria of Smith (1996) and Von Wirth and Striffler (2005).

28S rDNA sequences amplification: Total genomic DNA was extracted from each specimen using a Dneasy Tissue kit (Qiagen, Germany). The 28S primers for amplification were Hap1 (5’-GTACGTGAAACTGCTCAGAGGC-3’) and Hap2 (5’-GTCCAGGTTCGGGAATATTGACC-3’) which produced approximately 1600 bp PCR products. Hap3 (5’-TAAGGACGGGCACCGGAAG-3’) and Hap4 (5’-CGTGTTTCAAGACGGGTCGG-3’) produced 300 bp fragments within the above products. PCR cycling included an initial denaturation at 95°C for 3 min, followed by 30 cycles of denaturation at 94°C for 30 sec, annealing at 54°C for 30 sec and extension at 72°C for 1 min. Final extension was performed at 72°C for 10 min. DNA from the PCR products was purified using the QIAquick Gel Extraction Kit (Qiagen).

DNA sequencing analysis: DNA sequencing was performed by Macrogen DNA Sequencing Service, Korea. The 28S rDNA sequences have been deposited in the NCBI database (accession numbers KC881567 and KC881568). Phylogenetic analysis was performed using the bootstrap method with 1000 replications by the MEGA 5.10 software package (Tamura et al., 2011). The local similarity of the DNA sequences was analyzed using BLAST (http://blast.ncbi.nlm.nih.gov/ Blast.cgi).

RESULTS AND DISCUSSION

The phylogenetic tree of H. lividum and H. longipes showed a common cladistic form for mygalomorph relationships as assessed using partial 28S sequences (Fig. 2a). Molecular results confirmed that H. lividum and H. longipes were classified in the family Theraphosidae as a monophyletic group and clearly separated from other families within the mygalomorphs. Moreover, this phylogeny corresponded to the general taxon of tarantulas constructed by using morphological classification (Bertani, 2012; Zhu and Zhang, 2008). In contrast, the genus of Haplopelma was not monophyletic group within the subfamily of Ornithoctoninae. The H. lividum and H. longipes were more closely related to Cyriopagopus schioedtei than either was to H. schmidti. Hedin and Bond (2006) reported that a preliminary Mygalomorph phylogeny inferred from 18S and 28S rRNA genes conflicted with morphological hypotheses. Therefore, these molecular data did not support a morphological taxonomy between Haplopelma spp. and C. schioedtei.

Local alignments revealed that the partial 28S rDNA sequences of H. longipes shared 99% homology with those of H. lividum, H. schmidti and C. schioedtei (Fig. 2b). The H.lividum and H. longipes were clearly separated from Lasiodora parahybana and Chilobrachys huahini, sharing only 91 and 92% homology, respectively. The nucleotide sequences of H. lividum were 99, 98, 99, 91 and 91% of homologous to those of H. longipes, H. schmidti, C. schioedtei, L. parahybana and C. huahini, respectively. The sequences of H. longipes were 99, 99, 92 and 92% homologous to those of H. schmidti, C. schioedtei, L. parahybana and C. huahini, respectively. These results suggest that the 28S partial rDNA sequences of H. lividum and C. schioedtei are more closely conserved with each other than either is with those of H. schmidti.

Fig. 2(a-b): (a) Phylogenetic tree showing the relationships of H. lividum and H. longipes within 25 species of Mygalomorph based on the 28S rDNA gene and (b) Local similarity of the DNA sequences in Theraphosidae

The C. schioedtei (known as the Malaysian earth tiger tarantula) is a native species in Peninsular Malaysia (Song et al., 2002). Of the three Haplopelma considered here, H. schmidti is primarily found in Vietnam (Arabi et al., 2002); the distributions of H. lividum and H. longipes were explained in the introduction. In this study, however, the molecular evaluation was incongruent with the relationships suggested by taxonomy and geographical distributions since H. lividum exactly related to C. schioedtei by using 28S rDNA sequences.

CONCLUSION

In summary, the present study has determined partial 28S rDNA sequences for H. lividum and H. longipes and used these sequences to study the spiders’ phylogenetic relationships within the infraorder Mygalomorphae. The resulting molecular phylogeny was partially congruent with the phylogeny based on morphological descriptions of tarantulas but there were some inconsistencies between the two methods. The complete rDNA sequences remain to be evaluated and may help to resolve this incongruence.

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