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Research Article
 

Effects of Dietary Fermented Flammulina velutipes Mycelium on Performance and Egg Quality in Laying Hens



Sang-Bum Lee, Jintaek Im, Seon-Ku Kim, Youn-Chil Kim, Min-Jeung Kim, Jae-Seung Lee and Hong-Gu Lee
 
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ABSTRACT

This study was conducted to evaluate the effect of dietary Flammulina velutipes mycelium (FFVM) fermented with Bacillus subtilis A8-8 and Klebsiella sp. Sc on the performance of laying hens including the egg quality, the pathogenic bacterial (Escherichia coli and Salmonella spp.) counts in caecal contents and the NH3 production in excreta. One hundred eighty Hy-Line Brown hens were fed one of six diets, including a corn-soybean meal based control diet and control diets supplemented with FFVM at 1, 2, 3, 4, or 5% (n = 30 per experimental group). During the 5 week experimental period, laying hen performance, egg quality, pathogenic bacteria number in caecum and fecal NH3 gas production were estimated. Although there were no significant differences in egg production, feed intake and feed conversion among experimental groups, egg weight was significantly increased in the 1 and 3% FFVM groups compared with the control diet group (p<0.05). Interestingly, the 4% FFVM experimental group improved egg quality including albumen height, Haugh unit, egg shell weight and shell thickness, but not yolk color (p<0.05). On the other hand, the number of pathogenic bacteria such as Salmonella spp. and E. coli in caecum was significantly decreased by high levels of dietary FFVM supplementation (3-5%) (p<0.05). Early stage fecal NH3 gas production was also significantly suppressed by 3-5% dietary FFVM diets (p<0.05). Collectively, our results indicated that high level of dietary FFVM (4%) improves egg quality as well as suppressing pathogenic bacterial proliferation in the gastrointestinal tract and fecal NH3 gas emission.

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  How to cite this article:

Sang-Bum Lee, Jintaek Im, Seon-Ku Kim, Youn-Chil Kim, Min-Jeung Kim, Jae-Seung Lee and Hong-Gu Lee, 2014. Effects of Dietary Fermented Flammulina velutipes Mycelium on Performance and Egg Quality in Laying Hens. International Journal of Poultry Science, 13: 637-644.

DOI: 10.3923/ijps.2014.637.644

URL: https://scialert.net/abstract/?doi=ijps.2014.637.644

REFERENCES

1:  AOAC., 1995. Official Methods of Analysis. 16th Edn., Association of Official Analytical Chemists, Washington, DC., USA
Direct Link  |  

2:  Abe, F., N. Ishibashi and S. Shimamura, 1995. Effect of administration of Bifidobacteria and lactic acid bacteria to newborn calves and piglets. J. Dairy Sci., 78: 2838-2846.
CrossRef  |  Direct Link  |  

3:  Baba, E., S. Nagaishi, T. Fukuta and A. Arakawa, 1991. The role of intestinal microflora on the prevention of Salmonella colonization in gnotobiotic chickens. Poult. Sci., 70: 1902-1907.
PubMed  |  Direct Link  |  

4:  Chung, J.C., 1999. Production of liquid type strains of Flammulina velutipes and its technology. Mushroom, 3: 159-178.

5:  Davis, G.S. and K.E. Anderson, 2002. The effects of feeding the direct-fed microbial, primalac, on growth parameters and egg production in single comb white leghorn hens. Poult. Sci., 81: 755-759.
CrossRef  |  PubMed  |  Direct Link  |  

6:  Deaton, J.W., F.N. Reece and B.D. Lott, 1984. Effect of atmospheric ammonia on pullets at point of lay. Poult. Sci., 63: 384-385.
CrossRef  |  PubMed  |  Direct Link  |  

7:  Dunham, H.J., C. William, F.W. Edens, I.A. Casas and W.J. Dobrogosz, 1993. Lactobacillus reuteri immunomodulation of stress or associated disease in newly hatched chickens and turkeys. Poult. Sci., 72: 103-103.

8:  Fuller, R., 1989. Probiotics in man and animals. J. Applied Bacteriol., 66: 365-378.
CrossRef  |  PubMed  |  Direct Link  |  

9:  Hirai, Y., M. Ikeda, T. Murayama and T. Ohata, 1998. New monoterpentriols from the fruiting body of Flammulina velutipes. Biosci. Biotechnol. Biochem., 62: 1364-1368.
CrossRef  |  Direct Link  |  

10:  Hobbs, P.J., B.F. Pain, R.M. Kay and P.A. Lee, 1996. Reduction of odorous compounds in fresh pig slurry by dietary control of crude protein. J. Sci. Food. Agric., 71: 508-514.
CrossRef  |  Direct Link  |  

11:  Hooper, L.V., T. Midtvedt and J.I. Gordon, 2002. How host-microbial interactions shape the nutrient environment of the mammalian intestine. Annu. Rev. Nutr., 22: 283-307.
CrossRef  |  PubMed  |  Direct Link  |  

12:  Jang, B.S., H.I. Yun, S.C. Park, M.K. Kim, Y.W. Choi and T.K. Oh, 1999. Bacillus amyloliquefaciens DS11 phytase on phosphorus concentration in broiler gastrointestinal tract. Korean J. Vet. Public Health, 23: 45-45.

13:  Jin, L.Z., Y.W. Ho, N. Abdullah and S. Jalaludin, 1996. Influence of dried Bacillus subtilis and Lactobacilli cultures on intestinal microflora and performance in broilers. Asian-Australasian J. Anim. Sci., 9: 397-403.
Direct Link  |  

14:  Kalavathy, R., N. Abdullah, S. Jalaludin and Y.W. Ho, 2003. Effects of Lactobacillus cultures on growth performance, abdominal fat deposition, serum lipids and weight of organs of broiler chickens. Br. Poult. Sci., 44: 139-144.
CrossRef  |  Direct Link  |  

15:  Khachatourians, G.G., 1998. Agricultural use of antibiotics and the evolution and transfer of antibiotic-resistant bacteria. Can. Med. Assoc. J., 159: 1129-1136.
Direct Link  |  

16:  Kim, H.J., J.S. Woo, O.S. Kwon, B.J. Min and K.S. Shon et al., 2005. Effects of Bacillus subtilis supplementation on egg quality, blood characteristics and fecal NH3-N in laying hens. Korean J. Poult. Sci., 32: 9-14.
Direct Link  |  

17:  Kim, Y.O., H.K. Kim, K.S. Bae, J.H. Yu and T.K. Oh, 1998. Purification and properties of a thermostable phytase from Bacillus sp. DS11. Enzyme Microb. Technol., 22: 2-7.
CrossRef  |  Direct Link  |  

18:  Knap, I., A.B. Kehlet, M. Bennedsen, G.F. Mathis and C.L. Hofacre et al., 2011. Bacillus subtilis (DSM17299) significantly reduces Salmonella in broilers. Poult. Sci., 90: 1690-1694.
CrossRef  |  PubMed  |  

19:  Ko, W.C., W.C. Liu, Y.T. Tsang and C.W. Hsieh, 2007. Kinetics of winter mushrooms (Flammulina velutipes) microstructure and quality changes during thermal processing. J. Food Eng., 81: 587-598.
CrossRef  |  Direct Link  |  

20:  Kurtoglu, V., F. Kurtoglu, E. Seker, B. Coskun, T. Balevi and E.S. Polat, 2004. Effect of probiotic supplementation on laying hen diets on yield performance and serum and egg yolk cholesterol. Food Addit. Contam., 21: 817-823.
CrossRef  |  PubMed  |  Direct Link  |  

21:  Lee, S.B., Y.H. Choi, S.K. Cho, T.S. Shin and B.W. Cho et al., 2012. Effects of dietary Flammulina velutipes mycelium on broiler chick performance, pathogenic bacterial counts in caecal contents and amount of NH3 in excreta. J. Anim. Sci. Technol., 54: 341-347.
CrossRef  |  Direct Link  |  

22:  Lee, Y.S., I.H. Park, S.C. Ahn and Y.L. Choi, 2010. Isolation, purification and characterization of the β-xylosidase from Klebsiella sp. Sc. J. Life Sci., 20: 1801-1806.
CrossRef  |  Direct Link  |  

23:  Mee, B.J., 1984. The Selective Capacity of Pig Feed Additives and Growth Promotants for Coliform Resistance. In: Antimicrobials and Agriculture: The Proceedings of the 4th International Symposium on Antibiotics in Agriculture: Benefits and Malefits, Woodbine, M. (Ed.). Butterworths, London, UK., ISBN-13: 978-0-408-11155-3, pp: 349-358

24:  Nagaraja, K.V., D.A. Emercy, K.A. Jordan, J.A. Newman and B.S. Pomeroy, 1983. Scanning electron microscopic studies of adverse effects of ammonia on tracheal tissues of turkeys. Am. J. Vet. Res., 44: 1530-1536.
PubMed  |  

25:  Nahashon, S.N., H.S. Nakaue and L.W. Mitrosh, 1994. Production variables and nutrient retention in single comb White Leghorn laying pullets fed diets supplemented with direct-fed microbials. Poult. Sci., 73: 1699-1711.
PubMed  |  

26:  Nahashon, S.N., H.S. Nakaue and L.W. Mirosh, 1996. Performance of single comb white leghorn fed a diet supplemented with a live microbial during the growth and egg laying phases. Anim. Feed. Sci. Technol., 57: 25-38.
CrossRef  |  Direct Link  |  

27:  Na, J.C., B.G. Jang, S.H. Kim, J.H. Kim and S.K. Kim et al., 2005. Influence of feeding Flammuling veluipes media on productivity and egg quality in laying hens. Korean J. Poult. Sci., 32: 143-147.
Direct Link  |  

28:  Panda, A.K., M.R. Reddy, S.V. Rama Rao and N.K. Praharaj, 2003. Production performance, serum/yolk cholesterol and immune competence of white leghorn layers as influenced by dietary supplementation with probiotic. Trop. Anim. Health Prod., 35: 85-94.
CrossRef  |  Direct Link  |  

29:  Panda, A.K., S.S.R. Rao, M.V.L.N. Raju and S.S. Sharma, 2008. Effect of probiotic (Lactobacillus sporogenes) feeding on egg production and quality, yolk cholesterol and humoral immune response of White Leghorn layer breeders. J. Sci. Food Agric., 88: 43-47.
CrossRef  |  Direct Link  |  

30:  Park, J.W., T. Kim, D.J. Lim, H.B. Lee, Y.S. Joo and Y.I. Park, 2004. Antibacterial activities of mushroom liquid culture extracts against livestock disease-causing bacteria and antibiotic resistant bacteria. Korean J. Mycol. Sci., 32: 145-147.
CrossRef  |  Direct Link  |  

31:  Pelicano, E.R.L., P.A. de Souza, H.B.A. de Souza, F.R. Leonel, N.M.B.L. Zeola and M.M. Boiago, 2004. Productive traits of broiler chickens fed diets containing different growth promoters. Braz. J. Poult. Sci., 6: 177-182.
CrossRef  |  Direct Link  |  

32:  Reece, F.N., B.J. Bates and B.D. Lott, 1979. Ammonia control in broiler houses. Poult. Sci., 58: 754-760.
CrossRef  |  Direct Link  |  

33:  Ryu, K.S. and H.S. Park, 1998. Effect of feeding probiotics on performance and intestinal microflora of broiler chicks. Korean J. Poult. Sci., 25: 31-37.
Direct Link  |  

34:  Ryu, K.S., H.S. Park, M.S. Ryu, S.Y. Park, S.H. Kim and H.J. Song, 1999. Effect of feeding probiotics on performance and intestinal microflora of laying hens. Korean J. Poult. Sci., 26: 253-259.
Direct Link  |  

35:  Santoso, U., K. Tanaka and S. Ohtani, 1995. Effect of dried Bacillus subtilis culture on growth, body composition and hepatic lipogenic enzyme activity in female broiler chicks. Br. J. Nutr., 74: 523-529.
CrossRef  |  PubMed  |  Direct Link  |  

36:  Santoso, U., S. Ohtani, K. Tanaka and M. Sakaida, 1999. Dried Bacillus subtilis culture reduced ammonia gas release in poultry house. Asian-Australasian J. Anim. Sci., 12: 806-809.
Direct Link  |  

37:  Sen, S., S.L. Ingale, Y.W. Kim, J.S. Kim and K.H. Kim et al., 2012. Effect of supplementation of Bacillus subtilis LS 1-2 to broiler diets on growth performance, nutrient retention, caecal microbiology and small intestinal morphology. Res. Vet. Sci., 93: 264-268.
CrossRef  |  Direct Link  |  

38:  Smiderle, F.R., E.R. Carbonero, C.G. Mellinger, G.L. Sassaki, P.A.J. Gorin and M. Iacomini, 2006. Structural characterization of a polysaccharide and a β-glucan isolated from the edible mushroom Flammulina velutipes. Phytochemistry, 67: 2189-2196.
CrossRef  |  PubMed  |  Direct Link  |  

39:  Timmerman, H.M., A. Veldman, E. van den Elsen, F.M. Rombouts and A.C. Beynen, 2006. Mortality and growth performance of broilers given drinking water supplemented with chicken-specific probiotics. Poult. Sci., 85: 1383-1388.
CrossRef  |  Direct Link  |  

40:  Van Heugten, E. and T. Van Kempen, 1999. Methods may exist to reduce nutrient excretion. Feedstuffs, 71: 12-13.

41:  Wallinga, D. and D.G.S. Burch, 2013. Does adding routine antibiotics to animal feed pose a serious risk to human health? Br. Med. J., 9: 347-349.
CrossRef  |  PubMed  |  Direct Link  |  

42:  Li, L., C.L. Xu, C. Ji, Q. Ma, K. Hao, Z.Y. Jin and K. Li, 2006. Effects of a dried Bacillus subtilis culture on egg quality. Poult. Sci., 85: 364-368.
CrossRef  |  PubMed  |  Direct Link  |  

43:  Zhang, D., H. Hu, Q. Rao and Z. Zhao, 2011. Synergistic effects and physiological responses of selected bacterial isolates from animal feed to four natural antimicrobials and two antibiotics. Foodborne Pathog. Dis., 8: 1055-1062.
CrossRef  |  Direct Link  |  

44:  Zulkifli, I., N. Abdullah, N.M. Azrin and Y.W. Ho, 2000. Growth performance and immune response of two commercial broiler strains fed diets containing Lactobacillus cultures and oxytetracycline under heat stress conditions. Br. Poult. Sci., 41: 593-597.
CrossRef  |  Direct Link  |  

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