Subscribe Now Subscribe Today
Research Article
 

Isolation of Aeromonas hydrophila from Commercial Chickens in Jos Metropolis, Nigeria



Y.G. Dashe, M.A. Raji, P.A. Abdu, B.S. Oladele and D. Olarinmoye
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail
ABSTRACT

Aeromonas species are increasingly incriminated in clinical cases in livestock and humans in Nigeria and the world at large. This investigative study was carried out between November, 2010 and October, 2011 in Jos, Nigeria to determine the isolation rate of Aeromonas species in clinically sick and apparently healthy commercial chickens. A total of 2000 postmortem samples consisting of bone marrow, heart, liver, lung and spleen (400 each) were aseptically collected from 400 clinically sick chickens suspected to be suffering from various clinical conditions and cultured for Aeromonas organisms. Four hundred oro-pharyngeal swabs were also collected from 400 apparently healthy chickens for bacteriological analysis. Swab from each sample was cultured on 7% defibrinated sheep blood and MacConkey. From the bacteriological cultures of the bone marrow, heart and liver of the sick chickens, a total 11 (0.5%) Aeromonas hydrophila isolates were identified by biochemical characterization and Macrobact™ test. Aeromonas organism was not isolated at all from the apparently healthy chickens. The co-occurrence of Aeromonas hydrophila with other pathogens in the sick chickens could have contributed to the observed exacerbation of clinical signs and mortalities in some of the investigated flocks during the study period.

Services
Related Articles in ASCI
Similar Articles in this Journal
Search in Google Scholar
View Citation
Report Citation

 
  How to cite this article:

Y.G. Dashe, M.A. Raji, P.A. Abdu, B.S. Oladele and D. Olarinmoye, 2014. Isolation of Aeromonas hydrophila from Commercial Chickens in Jos Metropolis, Nigeria. International Journal of Poultry Science, 13: 26-30.

DOI: 10.3923/ijps.2014.26.30

URL: https://scialert.net/abstract/?doi=ijps.2014.26.30

REFERENCES
1:  Abdel-Gwad, A.M. and A.A. Abdel-Rahman, 2004. Isolation and significance of Aeromonas hydrophila group in farmed rabbits at Assuit governorate. Ass. Univ. Bull. Environ. Res., 7: 85-93.
Direct Link  |  

2:  Adeleke, E.O. and B.O. Omafuvbe, 2011. Antibiotic resistance of aerobic mesophilic bacteria isolated from poultry faeces. Res. J. Microbiol., 6: 356-365.
CrossRef  |  Direct Link  |  

3:  Awaad, M.H.H., M.E. Hatem, W.A.A. El-Ghany, A. El-Sawy and A. Fathi, 2011. Certain epidemiological aspects of Aeromonas hydrophila infection in chickens. J. Am. Sci., 7: 761-770.

4:  Barrow, G.I. and R.K.A. Felthan, 2004. Cowan and Steels Manual for the Identification of Medical Bacteria. 4th Edn., Cambridge University Press, UK., pp: 50-145.

5:  Barnes, H.J., J.P. Vaillancourt and W.B. Gross, 2003. Collibacillosis. In: Diseases of Poultry, Saif, Y.M. (Ed.). 11th Edn., Iowa State Press, Iowa, USA., pp: 631-656.

6:  Clark, G.M., A.F. Gangarosa and M. Thompson, 1973. Epidemiology of an international outbreak of Salmonella agona. Lancet, 302: 490-493.
CrossRef  |  Direct Link  |  

7:  CLSI, 2009. Procedure Manual for Laboratory Practice. 3rd Edn., Clinical and Laboratory Standards Institute, Wayne, Pennsylvania USA.

8:  Dallal, M.M.S., M.K.S. Yazdi and S. Avadisians, 2012. Study of prevalence and antibiotic resistance in Aeromonas species isolated from minced meat and chicken samples in Iran. Afr. J. Microbiol. Res., 6: 460-464.
Direct Link  |  

9:  Dashe, Y.G., M.A. Raji, P.A. Abdu and B.S. Oladele, 2013. Aeromonas hydrophila infections in chickens affected by fowl cholera in Jos Metropolis, Nigeria. Int. J. Microbiol. Immunol. Res., 1: 32-36.
Direct Link  |  

10:  Dashe, Y.D., M.A. Raji, P.A. Abdu, B.S. Oladele and M.Y. Sugun, 2013. Multidrug resistant Pasteurella multocida strains isolated from chickens with cases of fowl cholera in Jos, Nigeria. Int. J. Poult. Sci., 12: 596-600.
CrossRef  |  Direct Link  |  

11:  Franca, M., R.L. Walker, R. Kokka and H.L. Shivaprasad, 2009. Aeromonas species associated with necrotizing enteritis and septicemia in an adult male ostrich (Struthio camelus). Avian Dis., 53: 310-316.
CrossRef  |  Direct Link  |  

12:  Handfield, M., P. Simard, M. Couillard and R. Letarte, 1996. Aeromonas hydrophila isolated from food and drinking water: Hemagglutination, hemolysis and cytotoxicity for a human intestinal cell line (HT-29). Applied Environ. Microbiol., 62: 3459-3461.
Direct Link  |  

13:  Hassan, E.S.F., 2006. Incidence of hemolysin producing motile Aeromonas in some shellfish and their public health significance in Port-Said City. J. Applied Sci. Res., 2: 972-979.
Direct Link  |  

14:  Hazen, T.C., M.L. Racker, G.W. Esch and C.B. Fliermans, 1978. Ultra structure of red sore lesion on large mouth (Micropetrus salmoidsh) association of the ciliated Epistylis species and the bacterium Aeromonas hydrophila. J. Parasitol., 25: 551-555.

15:  Iloeje, N.P., 2001. A New Geography of Nigeria. Longman Nigeria Ltd., Nigeria, pp: 200.

16:  Kumar, S., P. Mukhopadhyay, M. Chatterjee, M. Bandyopadhyay, M. Bandyopadhyay, T. Ghosh and D. Samaddar, 2012. Necrotizing fasciitis caused by Aeromonas caviae. Avicenna J. Med., 2: 94-96.
CrossRef  |  PubMed  |  Direct Link  |  

17:  Mailafia, S., I. Ajogi and A.J. Nok, 2008. Occurrence and epidemiology of Aeromonas infections isolated from diarrhoeic patients in Zaria, Nigeria. Vom J. Vet. Sci., 5: 27-31.

18:  Martin-Carnahan, A.M. and S.W. Joseph, 2005. Genus I. Aeromonas Stanier 1943, 213AL. In: Bergey's Manual of Systematic Bacteriology, Brenner, D.J., N.R. Krieg, J.T. Staley and G.M. Garrity (Eds.). Springer, New York, pp: 557-578.

19:  Netys, K., G. Huys, M. Uyttendaele, J. Swings and J. Debevere, 2000. Incidence and identification of mesophilic Aeromonas spp. from retail foods. Lett. Applied Microbiol., 31: 359-363.
Direct Link  |  

20:  NPC, 2006. Population distribution by sex, state, LGAs and senatorial district: Census priority table (Vol. 3). Nigeria National Census, Nigeria.

21:  Nzeako, B. and N. Okafor, 2002. Bacterial enteropathogens and factors associated with seasonal episodes of gastroenteritis in Nsukka, Nigeria. Br. J. Biomed. Sci., 59: 76-79.
PubMed  |  

22:  Ocholi, R.A. and J.O. Kalejaiye, 1990. Aeromonas hydrophila as cause of hemorrhagic septicemia in a ground-hornbill (Bucorvus abyssinicus). Avian Dis., 34: 495-496.
PubMed  |  

23:  Ogbonna, A.C. and D.J. Harris, 2008. Thermal comfort in sub-Saharan Africa: Field study report in Jos-Nigeria. Applied Energy, 85: 1-11.
CrossRef  |  Direct Link  |  

24:  Okewole, P.A., P.S. Odeyemi, E.A. Irokanulo, L.L. Oyetunde and J. Chine, 1989. Cholangiohepatitis and biliary fibrosis in an adult rabbit with Aeromonas hydrophila infection. Bull. Anim. Health Prod. Afr., 37: 395-396.

25:  Okumura, K., F. Shoji, M. Yoshida, A. Mizuta, I. Makino and H. Higashi, 2011. Severe sepsis caused by Aeromonas hydrophila in a patient using tocilizumab: A case report. J. Med. Case Rep., Vol. 5. 10.1186/1752-1947-5-499

26:  Olarinmoye, A.O., O.O. Oladele, A.A. Adediji, U.G. Ntiwunka and G.O. Tayo, 2013. Antibiogram of avian pathogenic Escherichia coli isolates from commercial layers with colibacillosis in Southwest Nigeria. Malaysia J. Microbiol., 9: 317-325.
Direct Link  |  

27:  Okpokwasili, G.C. and J.N. Ogbulie, 2001. Biology and seasonality of Tilapia (Oreochromis niloticus) brown-patch syndrome. J. Trop. Aquacult., 16: 88-100.

28:  Portney, L.G. and M.P. Watkins, 2008. Foundation of Clinical Research Applications to Practice. 3rd Edn., Pearson Prentice Hall, London.

29:  Rippey, S.R. and V.J. Cabelli, 1979. Membrane filter procedure for enumeration of Aeromonas hydrophila in fresh waters. Applied Environ. Microbiol., 38: 108-113.
Direct Link  |  

30:  Saif, Y.M. and W.F. Busch, 1974. Aeromonas and Salmonella infections in Turkey poults. Report of the Ohio Agricultural Research and Development Center, Wooster, Ohio, USA., pp: 119-120.

31:  SPSS, 2004. Statistical package for social science (SPSS), Version 12.01. Chicago Incorporated, United States of America.

32:  Van Damme, L.R. and J. Vandepitte, 1980. Frequent isolation of Edwardsiella tarda and Pleisiomonas shigelloides from healthy Zairese freshwater fish: A possible source of sporadic diarrhea in the tropics. Applied Environ. Microbiol., 39: 475-479.
Direct Link  |  

33:  Zbikowski, A., P. Szeleszczuk, M. Rzewuska, E. Malicka and M. Binek, 2006. Epidemic deaths of mallard ducks after Aeromonas hydrophila infection. Medycyna Weterynaryjna, 62: 720-722.

©  2021 Science Alert. All Rights Reserved