Subscribe Now Subscribe Today
Research Article

Association of Mx Gene Genotype with Antiviral and Production Traits in Tolaki Chicken

Muhammad Amrullah Pagala, Muladno , Cece Sumantri and Sri Murtini
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Tolaki chicken is a kind of Indonesian local chickens, that belong to the ability of anti viral responses. This ability is controlled by the present of antiviral Mx (myxovirus resistance) gene. The Mx gene codes for a protein with antiviral activity. The objective of the study was to prove the tolaki chicken Mx gene genotype is associated with antiviral and production traits. Mx/Hpy 81 gene was genotyped in 103 tolaki chickens with PCR-RFLP. A total of 30 chickens were challenged with ND gen VIIb virus (104 CLD50)/chicken. PCR was used to amplify genomic DNA for Mx gene (299 bp). The amplimer was cut by Hpy 81 produce three genotypes: AA, AG and GG and two alleles: A allele (299 bp) and G allele (200 bp and 99 bp). Frequency of A allele (0.74) was higher than G allele (0.26). The all parameters of production traits in challenge test group were not significantly different in AA, AG and GG genotypes. The daily weight gain, feed intake and FCR were significantly different in AA, AG and GG genotypes of chickens control group. The parameters of antiviral traits showed that vitality of AA (50%) and AG (50%) of chickens were better then GG (10%) in challenge group. The vitality of AA (100%) and AG (100%) were better GG (33.33%) in control group. The study postulated that Mx gene genotype could be associated with production and antiviral traits in tolaki chicken. AA and AG genotype are more resistant and show better production than GG genotype.

Related Articles in ASCI
Search in Google Scholar
View Citation
Report Citation

  How to cite this article:

Muhammad Amrullah Pagala, Muladno , Cece Sumantri and Sri Murtini, 2013. Association of Mx Gene Genotype with Antiviral and Production Traits in Tolaki Chicken. International Journal of Poultry Science, 12: 735-739.

DOI: 10.3923/ijps.2013.735.739



  1. Alexander, D.J., 2001. Gordon Memorial Lecture. Newcastle disease. Br. Poult. Sci., 42: 5-22.
    PubMed  |  

  2. Alexander, D.J., 2003. Newcastle Disease, other Avian Paramyxoviruses and Pneumovirus Infections. In: Diseases of Poultry, Saif, Y.M., H.J. Barnes, J.R. Glisson, A.M. Fadly, L.R. Mc Dougald and D.E. Swayne (Eds.). 11th Edn., Iowa State Press, Ames, pp: 63-99

  3. Fulton, J.E., H.R. Juul-Madsen, C.M. Ashwell, A.M. McCarron, J.A. Arthur, N.P. O'Sullivan and R.L. Taylor Jr., 2006. Molecular genotype identification of the Gallus gallus major histocompatibility complex. Immunogenetics, 58: 407-421.
    CrossRef  |  

  4. Ko, J.H., H.K. Jin, A. Asano, A. Takada and A. Ninomiya et al., 2002. Polymorphisms and the differential antiviral activity of the chicken Mx gene. Genome Res., 12: 595-601.
    CrossRef  |  PubMed  |  Direct Link  |  

  5. Matzinger, S.R., T.D. Carol, J.C. Dutra, Z.M. Ma and C.J. Miller, 2013. Myxovirus resistance gen A (MxA) Expression supresses influenza A virus replication in alpha interferon treated primate cell. J. Virol., 87: 1150-1180.
    PubMed  |  

  6. Nei, M., 1987. Molecular Evolutionary Genetics. 1st Edn., Columbia University Press, New York, USA

  7. Otim, M.O., 2005. Newcastle disease in village poultry: Molecular and phylogenetic studies of the virus and disease epidemiology. Ph.D. Thesis, The Royal Veterinary and agricultural University (RVAU), Copenhagen, Denmark.

  8. Pavlovic, J., O. Haller and P. Staeheli, 1992. Human and Mouse Mx protein inhibit different step of the influenza virus multification cycle. J. Virol., 66: 2564-2569.
    Direct Link  |  

  9. Sambrook, J., E.F. Frotsch and T. Maniatis, 1989. Molecular Cloning. A Laboratory Manual. 2nd Edn., Cold Spring Harbor, Laboratory press, New York

  10. Sartika, T., S. Sulandari and M. Zein, 2010. Selection of Mx gene genotype as genetic marker for Avian Influenza resistance in Indonesian native chicken. Biomed. Cent. Proceed., Vol. 5.
    CrossRef  |  

  11. Sironi, L., P. Ramelli, J.L. Williams and P. Mariani, 2010. PCR-RFLP genotyping protocol for chicken Mx gene G/A polymorphism associated with the S631N mutation. Genet. Mol. Res., 9: 1104-1108.
    CrossRef  |  PubMed  |  

  12. Watanabe, T., 2003. Genomic analysis of antiviral resistant Mx gene in the chicken. Proceedings of the International Workshop on Animal Genome Analysis, September 16-17, 2003, Sapporo, Japan -

©  2022 Science Alert. All Rights Reserved