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Research Article

Screening Blood Samples to Estimate When Oxytetracycline Residues Exceed Regulatory Tolerances in Poultry Muscle

I. Reyes-Herrera, M.J. Schneider, P.J. Blore and D.J. Donoghue
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Presence of antibiotic residues in edible animal products is a human food safety concern. To address this potential problem, many governments sample edible tissues, such as muscle, to monitor for residues. Alternatively, antibiotic residue concentrations could be screened in blood which is readily available during carcass processing. To determine if blood concentrations are predictive of muscle concentrations, 252 market aged broilers were dosed with Oxytetracycline (OTC) in water at three doses: the maximum OTC approved dose for broilers (800 mg/gal) or five or ten times that dose. Blood and muscle samples were collected before initial dosing (0 hour, controls), during dosing at 1, 3, 6, 12, 24, 48, 96 or 144 hours and at 12, 24, 36, 48 or 60 hours after drug withdrawal. Concentrations in blood and muscle tissues followed similar time: concentration patterns, peaking 24 hours after initial dosing (396±9 vs. 557±37 ppb; 443±48 vs. 1846±58 ppb or 2447±67 vs. 3210±36 ppb for the 1, 5 or 10x doses in blood vs. muscle, respectively) and declined rapidly after withdrawal. These data suggest blood samples may be used to predict OTC concentrations in muscle as a screening procedure for OTC residues in poultry.

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  How to cite this article:

I. Reyes-Herrera, M.J. Schneider, P.J. Blore and D.J. Donoghue, 2013. Screening Blood Samples to Estimate When Oxytetracycline Residues Exceed Regulatory Tolerances in Poultry Muscle. International Journal of Poultry Science, 12: 348-352.

DOI: 10.3923/ijps.2013.348.352



  1. Bruhn, C.M., 1999. Consumer perceptions and concerns about food contaminants. Adv. Exp. Med. Biol., 459: 1-7.
    PubMed  |  

  2. Chiesa, O.A., J. von Bredow, D. Heller, C. Nochetto, M. Smith, K. Moulton and M. Thomas, 2006. Use of tissue-fluid correlations to estimate gentamicin residues in kidney tissue of Holstein steers. J. Vet. Pharmacol. Ther., 29: 99-106.
    CrossRef  |  

  3. Clement, R.P., 1995. Preclinical drug metabolism programs for food-producing animals. Toxicol. Pathol., 23: 209-216.
    PubMed  |  

  4. De Wasch, K., L. Okerman, H. De Brabander, J. Van Hoof, S. Croubels and P. De Backer, 1998. Detection of residues of tetracycline antibiotics in pork and chicken meat: Correlation between results of screening and confirmatory tests. Analyst, 123: 2737-2741.
    CrossRef  |  Direct Link  |  

  5. Delis, G., G. Batzias, G. Kounenis and M. Koutsoviti-Papadopoulou, 2009. Application and validation of a LC/fluorescence method for the determination of amoxicillin in sheep serum and tissue cage fluid. J. Pharm. Biomed. Anal., 49: 375-380.
    CrossRef  |  PubMed  |  

  6. Donoghue, D.J., H. Hairston, S.A. Gaines, M.J. Bartholomew and A.M. Donoghue, 1996. Modeling residue uptake by eggs. 1. Similar drug residue patterns in developing yolks following injection with ampicillin or oxytetracycline. Poult. Sci., 75: 321-328.
    PubMed  |  

  7. Donoghue, D.J., 2003. Antibiotic residues in poultry tissues and eggs: Human health concerns? Poult. Sci., 82: 618-621.
    CrossRef  |  PubMed  |  Direct Link  |  

  8. Fink-Gremmels, J. and A.S. van Miert, 1994. Veterinary drugs: Disposition, biotransformation and risk evaluation. Analyst, 119: 2521-2528.
    PubMed  |  

  9. FSIS, 2009. Poultry products inspection act: Title 21 food and drugs. Chapter 10: Poultry and Poultry Products Inspection.

  10. FSIS-NRP, 2009. Food safety and inspection service. 2009 FSIS National Residue Program Scheduled Sampling Plans.

  11. Haasnoot, W., G.O. Korsrud, G. Cazemier, F. Maneval, H. Keukens and J. Nouws, 1996. Application of an enzyme immunoassay for the determination of sulphamethazine (sulphadimidine) residues in swine urine and plasma and their use as predictors of the level in edible tissue. Food Addit. Contam., 13: 811-821.
    PubMed  |  

  12. Health Canada, 2002. Setting standards for Maximum Residue Limits (MRLs) of veterinary durgs used in food-producing animals.

  13. MHLW, 2003. First draft of provisional maximum residue limits for agricultural chemicals in foods. Ministry of Health, Labor and Welfare.

  14. Oka, H., Y. Ito, Y. Ikai, H. Matsumoto and K. Kato et al., 2001. Survey of residual tetracyclines in kidneys of diseased animals in Aichi Prefecture, Japan (1985-1997). J. AOAC Int., 84: 350-353.
    PubMed  |  

  15. Okerman, L., K. de Wasch and J. van Hoof, 1998. Detection of antibiotics in muscle tissue with microbiological inhibition tests: Effects of the matrix. Analyst, 123: 2361-2365.
    PubMed  |  

  16. Okerman, L., S. Croubels, M. Cherlet, K. De-Wasch, P. De-Backer and J. Van-Hoof, 2004. Evaluation and establishing the performance of different screening tests for tetracycline residues in animal tissues. Food Addit. Contam., 21: 145-153.
    PubMed  |  Direct Link  |  

  17. Reyes-Herrera, I., M.J. Schneider, P.J. Blore and D.J. Donoghue, 2011. The relationship between blood and muscle samples to monitor for residues of the antibiotic enrofloxacin in chickens. Poult. Sci., 90: 481-485.
    CrossRef  |  

  18. Ridgway, K., S.P.D. Lallje and R.M. Smith, 2007. Sample preparation techniques for the determination of trace residues and contaminants in foods. J. Chromatograph A, 1153: 36-53.
    CrossRef  |  

  19. Rigamonti, A.E., S.G. Cella, N. Marazzi, L. Di Luigi, A. Sartorio and E.E. Muller, 2005. Growth hormone abuse: Methods of detection. Trends Endocrinol. Metab., 16: 160-166.
    CrossRef  |  

  20. Rivier, L., 2000. Techniques for analytical testing of unconventional samples. Best Practice Res. Endocrinol. Metabolism, 14: 147-165.

  21. Schneider, M.J., I. Reyes-Herrera and D.J. Donoghue, 2007. Evaluation of serum as a potential matrix for multiresidue determination of fluoroquinolone antibiotics in chicken using liquid chromatography-fluorescence-mass spectrometry(n). J. AOAC Int., 90: 1716-1723.
    PubMed  |  

  22. Schneider, M.J., S.E. Braden, I. Reyes-Herrera and D.J. Donoghue, 2007. Simultaneous determination of fluoroquinolones and tetracyclines in chicken muscle using HPLC with fluorescence detection. J. Chromatogr B, 846: 8-13.
    CrossRef  |  

  23. Schneider, M.J. and S.J. Lehotay, 2008. A comparison of the FAST, Premi and KIS tests for screening antibiotic residues in beef kidney juice and serum. Anal. Bioanal. Chem., 390: 1775-1779.
    CrossRef  |  

  24. Schneider, M.J., K. Mastovska, S.J. Lehotay, A.R. Lightfield, B. Kinsella and C.E. Shultz, 2009. Comparison of screening methods for antibiotics in beef kidney juice and serum. Anal. Chim. Acta, 637: 290-297.
    CrossRef  |  

  25. Tilman, D., K.G. Cassman, P.A. Matson, R. Naylor and S. Polasky, 2002. Agricultural sustainability and intensive production practices. Nature, 418: 671-677.
    CrossRef  |  Direct Link  |  

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