Yousef Doustar
Department of Pathobiology, Tabriz Branch, Islamic Azad University, Tabriz, Iran
Daryoush Mohajeri
Department of Pathobiology, Tabriz Branch, Islamic Azad University, Tabriz, Iran
Younes Anzabi
Department of Microbiology, Tabriz Branch, Islamic Azad University, Tabriz, Iran
Mehrdad Nazeri
Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran
ABSTRACT
Influenza virus produces cell death in animals and human. Since cell death can be caused by either necrosis or apoptosis. We investigated the types of cell death that occur in chickens infected with avian influenza virus, A/chicken/Iran/772/2000(H9N2). In experimental study 60 SPF chickens at 3 weeks old were divided to two groups. The first group was infected with 107.5 EID50 titer of the virus intranasaly and the second group was treated with saline normal. Following 72 hrs, renal tissues were collected and fixed in 10% formalin solution. The prepared microscopic sections with the thickness of 5-6 micron were stained using TUNEL method. In comparison to the control group, there were significant mean difference of apoptotic cells in renal tubular cells of the infected group (p<0.005). We demonstrated that A/chicken/Iran/772/2000 (H9N2) is able to induce apoptosis in renal tubular cells.
PDF References Citation
How to cite this article
Yousef Doustar, Daryoush Mohajeri, Younes Anzabi and Mehrdad Nazeri, 2011. Experimental Study of Renal Tubular Cells Apoptosis Subsequent to Infectious by Influenza Virus (H9N2) in SPF Chickens. International Journal of Poultry Science, 10: 891-894.
DOI: 10.3923/ijps.2011.891.894
URL: https://scialert.net/abstract/?doi=ijps.2011.891.894
DOI: 10.3923/ijps.2011.891.894
URL: https://scialert.net/abstract/?doi=ijps.2011.891.894
REFERENCES
- Brydon, E.W.A., S.J. Morris and C. Sweet, 2005. Role of apoptosis and cytokines in influenza virus morbidity. FEMS Microbiol. Rev., 29: 837-850.
CrossRef - Chai J., C. Du, J.W. Wu, S. Kyin, X. Wang and Y. Shi, 2000. Structural and biochemical basis of apoptotic activation by smac/DIABLO. Nature, 406: 855-862.
CrossRefDirect Link - Frankfurt, O.S. and A. Krishan, 2001. Identification of apoptotic cells by formamide: Induced DNA denaturation in condensed chromatin. J. Histochem. Cytochem., 49: 369-378.
PubMedDirect Link - Ito, T., Y. Kobayashi, T. Morita, T. Horimoto and Y. Kawaoka, 2002. Virulent influenza a viruses induces apoptosis in chickens. Virus Res., 84: 27-35.
CrossRefPubMedDirect Link - Keogh, S.A., H. Walczak, L. Bouchier-Hayes and SJ. Martin, 2000. Failure of Bcl-2 to block Cytochrome C redistribution during Trail: Induced apoptosis. FEBS Lett., 471: 93-98.
PubMedDirect Link - Liu, J., K. Okazaki, H. Ozaki, Y. Sakoda, Q. Wu, F. Chen and H. Kida, 2003. H9N2 influenza viruses prevalent in poultry in China are phylogenetically distinct from A/quail/Hong Kong/G1/97 presumed to be the donor of the internal protein genes of the H5N1 Hong Kong/97 virus. Avian Pathol., 32: 551-560.
- Ravi, R., G.C. Bedi, L.W. Engstrom, Q. Zeng and B. Mookerjee et al., 2001. Regulation of death receptor expression and TRAIL/Apo2L- induced apoptosis by NF-κB. Nat. Cell Biol., 3: 409-416.
PubMedDirect Link - Schultz-Cherry, S., N. Dybdahl-Sissoko, G. Neumann, Y. Kawaoka and V.S. Hinshaw, 2001. Influenza Virus NS1 protein induces apoptosis in cultured cells. J. Virol., 75: 7875-7881.
CrossRefDirect Link - Schultz-Cherry, S., M. Koci, E. Thompson and T.M. Tumpey, 2003. Examining the cellular pathways involved in influenza viras induced apoptosis. Avian Dis., 47: 968-971.
PubMedDirect Link - Suarez, D.L. and S. Schultz-Cherry, 2000. Immunology of avian influenza Virus: A Review. Dev. Comp. Immunol., 24: 269-283.
CrossRef - Zambon, M.C., 2001. Pathogenesis of influenza in humans. Rev. Med. Virol., 11: 227-241.
PubMedDirect Link