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Research Article

Effect of Dietary Supplementation with Different Levels of Arginine on Some Blood Traits of Laying Hens

Ali S.A. Al-Hassani
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This experiment was carried out at the Field of Poultry, Department of Animal Resources, College of Agriculture, University of Baghdad during the period from 1/5/2011 until 5/7/2011 to study the effect of adding arginine to laying hens diet on certain blood traits. A total of 100 Brown Lohmann laying hens chicken, 38 weeks of age were randomly distributed on four treatment groups with 25 hens for each treatment. Treatment groups were: T1: Bird fed diet with no additional arginine (control group); T2, T3 and T4: Birds fed diet supplemented with 0.4, 0.7 and 0.9% respectively. Therefore, the total amounts of arginine in the four treatments (T1, T2, T3 and T4) become 1.1, 1.5, 1.8, 2.0% respectively. Results of this experiment revealed that there were no significant differences (p>0.05) between treatment groups regarding the total of Red Blood Cells Count (RBC) as well as with respect to each of the Hemoglobin concentration (Hb) and Packed Cell Volume (PCV). It was also noticed that there were no significant differences (p>0.05) between treatment groups concerning each of Mean Cell Volume (MCV), Mean Cell Hemoglobin (MCH) and Mean Corpuscular Hemoglobin Concentration (MCHC). Moreover, results of this study also denoted that supplementing ration of laying hens with different levels of arginine (T2, T3 and T4) resulted in significant increase (p<0.05) in total White Blood Cells Count (WBC) and percentage of Heterophil (H) cells and significant decrease (p<0.05) respecting percentages of Lymphocyte (L) cells and monocyte cells and H/L ratio as compared with control group (T1). In conclusion, adding arginine to the diet of laying hens at levels higher than the levels recommended by the NRC did not have a negative effect on physiological performance of birds, as indicated by the non significant differences between treatment groups as regards RBC, PCV, Hb, MCV, MCH and MCHC. However, supplementing arginine to the diet of laying hens resulted in enhancement of immune response as indicated by significant increase in WBC in comparison with control group. On the other hand, adding arginine to laying hens ration didn’t cause any stress on birds as indicate by the significant decrease in H/L ratio as compared with control group.

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  How to cite this article:

Ali S.A. Al-Hassani , 2011. Effect of Dietary Supplementation with Different Levels of Arginine on Some Blood Traits of Laying Hens. International Journal of Poultry Science, 10: 705-709.

DOI: 10.3923/ijps.2011.705.709


1:  Abdel-Maksoud, A., F. Yan, S. Cerrate, Z. Wang and P.W. Waldroup, 2010. Effect of arginine levels and source and level of methionine on performance of broiler 0 to 18 days of age. Int. J. Poult. Sci., 91: 14-20.
Direct Link  |  

2:  Al-Daraji, H.J., W.K. Al-Hayani and A.S. Al-Hassani, 2008. Avian hematology. Ministry of Higher Education and Scientific Research, College of Agriculture, University of Baghdad, Baghdad, Iraq.

3:  Archer, R.K., 1965. Hematological Techniques for Use on Animals. Blackwell Scientific Publications, Oxford.

4:  Atakisi, O., E. Atakisi and A. Kart, 2009. Effects of dietary zinc and L-arginine supplementation on total antioxidants capacity, lipid peroxidation, nitric oxide, egg weight and blood biochemical values in Japanese quails. Biol. Trace Elem. Res., 132: 136-143.
CrossRef  |  PubMed  |  Direct Link  |  

5:  Billiar, T.R., 1995. Nitric oxide. Novel biology with clinical relevance. Ann. Surg., 221: 339-349.
Direct Link  |  

6:  Brake, J., P. Ferket, D. Balnave, I. Gorman and J.J. Dibner, 1994. Optimum arginine: Lysine ratio changes in hot weather. Proceedings of 21th Annual Carolina Poultry Nutrition Conference, December 7-8, 1994, Charlotte, NC., USA., pp: 82-104.

7:  Duncan, D.B., 1955. Multiple range and multiple F tests. Biometrics, 11: 1-42.
CrossRef  |  Direct Link  |  

8:  Emadi, M., F. Jahanshiri, F.A. Jalalian, K. Kaveh and M.H. Bejo et al., 2010. Immunostimulatory effects of arginine in broiler chickens challenged with vaccine strain of infectious bursal disease virus. J. Anim. Vet. Adv., 9: 594-600.
CrossRef  |  Direct Link  |  

9:  Grazi, E., M. Ermes and B. Ginacarlo, 1975. On the control of arginine metabolism in chicken kidney and liver. Eur. J. Biochem., 60: 431-436.
PubMed  |  

10:  Kwak, H., R.E. Austic and R.R. Dietert, 1999. Influence of dietary arginine concentration on lymphoid organ growth in chickens. Poult. Sci., 78: 1536-1541.
PubMed  |  Direct Link  |  

11:  Knowles, R.G. and S. Moncada, 1994. Nitric oxide synthases in mammals. Biochem. J., 298: 249-258.
PubMed  |  

12:  Martin, G., A.O. Lenderer, A. Chori, N. Neuenkirchen and S. Dettwiler et al., 2010. Arginine methylation in subunit of mammalian pre-mRNA cleavage factore 1. RNA, 16: 1646-1659.
PubMed  |  Direct Link  |  

13:  Mendes, R.A.C., H. Hansse, K. Kiessling, N.B. Roberts, G.E. Mann and J.C. Ellory, 1997. Transport of L-arginine and the nitric oxide inhibitor NG-monometyl-L-arginine in human erythrocyte in chronic renal failure. Clin. Sci., 93: 57-64.
PubMed  |  Direct Link  |  

14:  NRC., 1994. Nutrient Requirement of Poultry. 8th Edn., National Academy Press, Washington, DC., USA.

15:  Natt, P.M. and C.A. Herrik, 1952. A new blood diluent for counting the erythrocytes and leucocytes of chicken. Poult. Sci., 31: 735-738.

16:  SAS, 2000. Propriety Software Release 8.1. Statistical Analysis Systems Institute Inc., Cary, North Carolina, USA.

17:  Snyder, J.M., W.D. Morrison and H.M. Scott, 1956. The arginine requirement of chicks fed purified and corn-soya diets. Poult. Sci., 35: 852-855.

18:  Stechmiller, J.K., B. Langkamp-Henken, B. Childress, K.A. Herrlinger-Garcia, J. Hudgens and L. Tian et al., 2005. Arginine supplementation does not enhance serum nitric oxide levels in elderly nursing home residents with pressure ulcers. Biol. Res. Nurs., 6: 289-299.
PubMed  |  

19:  Tapiero, H., G.N. Ba, P. Couvreur and K.D. Tew, 2002. Polyunsaturated fatty acids (PUFA) and eicosanoids in human health and pathologies. Biomed. Pharmacotherap., 56: 215-222.
CrossRef  |  Direct Link  |  

20:  Varley, H., A.H. Gowenlock and M. Bell, 1980. General Topics and Commoner Tests Practical Clinical Biochemistry. 5th Edn., William Heinemann Medical Books Ltd., London.

21:  Veldkamp, T., R.P. Kwakkel, P.R. Ferket, P.C.M. Simons, J.P.T. Noordhuizen and A. Pijpers, 2000. Effects of ambient temperature, arginine-to-lysine ratio and electrolyte balance on performance, carcass and blood parameters in commercial male turkeys. Poult. Sci., 79: 1608-1616.
PubMed  |  

22:  Wietlake, A.W., A.G. Hogan, B.L.O. Dell and H.L. Kempaster, 1954. Amino acid deficiencies of casein as source of protein for the chick. J. Nutr., 52: 311-323.
PubMed  |  Direct Link  |  

23:  Wu, G., L.A. Jaeger, F.W. Bazer and J.M. Rhoads, 2004. Arginine deficiency in preterm infants: Biochemical mechanisms and nutritional implications. J. Nutr. Biochem., 15: 442-451.
PubMed  |  

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