Subscribe Now Subscribe Today
Research Article
 

Evaluation of Experimental Vaccination Against Newcastle Disease in Lovebirds (Agapornis roseicollis): Investigation of the State of Virus Carrier



Gislaine Regina Vieira Martins, Elizabeth Moreira dos Santos Schmidt, Adriano Torres Carrasco, Antonio Carlos Paulillo and Janine Denadai
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail
ABSTRACT

The aim of this study was to evaluate the importance of vaccination against Newcastle Disease (ND) in lovebirds (Agapornis roseicollis) and to investigate the state of carrier of the virus (NDV) in this species. There were used 48 lovebirds, distributed at random into 4 experimental groups: GI (Ulster 2C strain), GII (B1 strain), GIII (LaSota strain) and GIV (non-vaccinated group). At 12 months of age, all groups were challenged with a pathogenic virus (NDV) suspension (EID50 = 108.15/0.1 mL) and a group of Specific-Pathogen-Free (SPF) chicks were used as control of the virus. Cloacal swabs from each bird were collected after 9, 14 and 21 days post-challenge for detection of genome viral excretion by Reverse Transcription Polymerase Chain Reaction RT-PCR. Lovebirds of GI, GII and GIII did not demonstrate any signs of ND. They were refractory to the clinical disease. In lovebirds from the control group, NDV genome was detected 9 and 21 days after challenge. Therefore it was demonstrated the state of carrier of NDV by lovebirds. In birds from the vaccinated groups, genome viral excretion was not detected by RT-PCR. It was also demonstrated the importance of the vaccination in the suppression of the state of virus carrier of ND in lovebirds.

Services
Related Articles in ASCI
Similar Articles in this Journal
Search in Google Scholar
View Citation
Report Citation

 
  How to cite this article:

Gislaine Regina Vieira Martins, Elizabeth Moreira dos Santos Schmidt, Adriano Torres Carrasco, Antonio Carlos Paulillo and Janine Denadai, 2011. Evaluation of Experimental Vaccination Against Newcastle Disease in Lovebirds (Agapornis roseicollis): Investigation of the State of Virus Carrier. International Journal of Poultry Science, 10: 694-696.

DOI: 10.3923/ijps.2011.694.696

URL: https://scialert.net/abstract/?doi=ijps.2011.694.696

REFERENCES
1:  Alexander, D.J. and D.A. Senne, 2008. Newcastle Disease. In: Diseases of Poultry, Saif, Y.M. (Ed.). 12th Edn., Blackwell Publishing, Iowa, pp: 75-100.

2:  CFR, 1993. Animal and Animal Products. National Archives and Records Administrator, Washington, DC., pp: 818.

3:  Forshaw, J.M., 1989. Parrots of the World. Willoughby, Lansdowne, Pages: 450.

4:  Kaleta, E.F. and C. Baldauf, 1988. Newcastle Disease in Free-Living and Pets Birds. In: Newcastle Disease, Alexander, D.J. (Ed.). Kluwer Academic Publishers, Boston, MA., USA., pp: 197-246.

5:  OIDE., 1996. Manual for Animal Disease Reporting to the OIE. World Organization for Animal Health, Paris.

6:  Toyoda, T., T. Sakagushi, H. Hirota, B. Gotoh, K. Kuma, T. Miyata and Y. Nagai, 1989. Newcastle disease virus evolution: II. Lack of gene recombination in generating virulent and avirulent strains. Virology, 169: 273-282.
CrossRef  |  

©  2021 Science Alert. All Rights Reserved