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Research Article

Growth and Survival of Lactic Acid Bacteria Isolated from Byproduct of Virgin Coconut Oil as Probiotic Candidate for Poultry

Husmaini , M.H. Abbas, E. Purwati, A. Yuniza and A.R. Alimon
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The main objective of this research is to investigate the potential of LABs isolated from processing byproduct of the VCO in in vitro conditions for use as probiotics in poultry. Forty eight LABs were isolated and four of them have been selected for further study i.e. Lh1, Lh2, Lh3 and Lh4. A series of tests carried out by studying the ability of bacteria to survive at 37 and 42oC, tolerance of LAB at pH 2, 0, 5, 7 and 7, 0 and tolerance to gastric juice as well as sensitivity to several antibiotics commonly was given to poultry. The survival of LABs was evaluated after 15, 30, 60, 90 and 120 and 300 min of incubation. The sensitivity test to antibiotics was performed by Muller Hinton′s agar. All the bacteria showed tolerance and ability to grow at pH 5 and 7, but only Lh4 enabled to tolerate at pH 2. All of LAB can grow at gastric juice stimulated. Lh4 was not sensitive to all antibiotics (clear zones: 0.33 mm) but the other LABs were sensitive (clear zones: 5-12 mm). The conclusion of this research is the ability of LABs to grow in in vitro conditions varies. The Lh4 has demonstrated its ability to grow and the best survival with the OD (λ = 580) is 1.99 after 300 min of incubation at pH 2 and has shown the most resistant to all antibiotics tested with a wide clear zone 0.33 mm, hence potentially be used for probiotic in poultry.

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  How to cite this article:

Husmaini , M.H. Abbas, E. Purwati, A. Yuniza and A.R. Alimon, 2011. Growth and Survival of Lactic Acid Bacteria Isolated from Byproduct of Virgin Coconut Oil as Probiotic Candidate for Poultry. International Journal of Poultry Science, 10: 309-314.

DOI: 10.3923/ijps.2011.309.314



  1. Asahara, T., K. Shimizu, K. Nomoto, T. Hamabata, A. Ozawa and Y. Takeda, 2004. Probiotic bifidobacteria protect mice from lethal infection with Shiga toxin-producing Escherichia coli O157: H7. Infect. Immun., 72: 2240-2247.
    CrossRef  |  Direct Link  |  

  2. Boonkumklao, P., P. Kongthong and A. Assavanig, 2006. Acid and bile tolerance of Lactobacillus thermotolerans, a novel species isolated from chicken feces. Kasetsart J. Nat. Sci., 40: 13-17.
    Direct Link  |  

  3. Chou, L.S. and B. Weimer, 1999. Isolation and characterization of acid and bile tolerant isolated from strains of Lactobacillus acidophilus. J. Dairy Sci., 82: 23-31.
    PubMed  |  

  4. Conway, P.L., S.L. Gorback and B.R. Goldin, 1987. Survival of lactic acid bacteria adhesion to intestinal cells. J. Dairy Sci., 70: 1-12.
    Direct Link  |  

  5. Dawson, W.R. and G.C. Whittow, 2000. Regulation of Body Temperature. In: Sturkie's Avian Physiology, Whittow, G.C. (Ed.). Academic Press, New York, USA., ISBN: 978-0-12-747605-6, pp: 343-390

  6. Denbow, D.M., 2000. Gastointestinal Anatomy and Physiology. In: Sturkie's Avian Physiology, Whittow, G.C., (Ed.), Academic Press, San Diego, pp: 299-325

  7. Denli, M., F. Okan and K. Celik, 2003. Effect of dietary probiotic, organic acid and antibiotic supplementation to diets on broiler performance and carcass yield. Pak. J. Nutr., 2: 89-91.
    CrossRef  |  Direct Link  |  

  8. Duke, G.E., 1977. Avian Digestion. In: Physiology of Domestic Animals, 9th Edn., Duke, G.E. (Ed.), Cornell University Press, Ithaca, London, pp: 313-320

  9. Dunne, C., L. O'Mahony, L. Murphy, G. Thornton and D. Morrissey et al., 2001. In vitro selection criteria for probiotic bacteria of human origin: Correlation with in vivo findings. Am. J. Clin. Nutr., 73: 386s-392s.
    PubMed  |  Direct Link  |  

  10. Erkkila, S., M. Venalainen, S. Hielm, E. Petaja, E. Puolanne and T. Mattila-Sandholm, 2000. Survival of Escherichia coli O157: H7 in dry sausage fermented by probiotic lactic acid bacteria. J. Sci. Food Agric., 80: 2101-2104.
    CrossRef  |  Direct Link  |  

  11. Fuller, R., 1989. Probiotics in man and animals. J. Applied Bacteriol., 66: 365-378.
    CrossRef  |  PubMed  |  Direct Link  |  

  12. Gibson, G.R. and R. Fuller, 2000. Aspects of in vitro and in vivo research approaches directed toward identifying probiotics and prebiotics for human use. J. Nutr., 130: 391S-395S.
    CrossRef  |  Direct Link  |  

  13. Gilliland, S.E., T.E. Staley and L.J. Bush, 1984. Importance of bile tolerance of Lactobacillus acidophilus used as a dietary adjunct. J. Dairy Sci., 67: 3045-3051.
    CrossRef  |  PubMed  |  Direct Link  |  

  14. Havenaar, R., B. Ten Brink and J.H.J. Huisin Veld, 1992. Selection of Strains for Probiotics Use. In: The Scientific Basis, Fuller, R. (Ed.). Chapman and Hall, London, pp: 209-224

  15. Higgins, S.E., J.P. Higgins, A.D. Wolfenden, S.N. Henderson, A. Torres-Rodriguez, G. Tellez and B. Hargis, 2008. Evaluation of a Lactobacillus-based probiotic culture for the reduction of Salmonella enteritidis in neonatal broiler chicks. Poult. Sci., 87: 27-31.
    CrossRef  |  

  16. Higgins, J.P., S.E. Higgins, A.D. Wolfenden, S.N. Henderson and A. Torres-Rodriguez et al., 2010. Effect of lactic acid bacteria probiotic culture treatment timing on Salmonella enteritidis in neonatal broilers. Poult. Sci., 89: 243-247.
    CrossRef  |  Direct Link  |  

  17. Holzapfel, W.H., P. Haberer, R. Geisen, J. Bjorkroth and U. Schillinger, 2001. Taxonomy and important features of probiotic microorganisms in food and nutrition. Am. J. Clin. Nutr., 73: 365S-373S.
    PubMed  |  Direct Link  |  

  18. Hungate, R.E., 1969. A Roll Tube Method for Cultivation of Strict Anaerobes. In: Methods in Microbiology, Norris, J.R. and D.W. Ribbons (Eds.). Academic Press, New York, USA., pp: 117-132

  19. Husmaini, M., H. Abbas and L. Putri, 2007. The effect of blondo (by product of VCO processing) in rations on the broiler performances. J. Peternakan Indonesia, 11: 143-151.

  20. Kim, Y.G., T. Ohta, T. Takahashi, A. Kushiro and K. Nomoto et al., 2006. Probiotic Lactobacillus casei activates innate immunity via NF-κB and p38 MAP kinase signaling pathways. Microbes. Infect., 8: 994-1005.
    CrossRef  |  PubMed  |  Direct Link  |  

  21. Leeson, S. and J.D. Summers, 2001. Scott's Nutrition of the Chicken. 4th Edn., Guelph, Ontario, Canada

  22. Niamsup, P., I.N. Sujaya, M. Tanaka, T. Sone and S. Hanada et al., 2003. Lactobacillus thermotolerans sp. nov., a novel thermotolerant species isolated from chicken faeces. Int. J. Syst. Evol. Microbiol., 53: 263-268.
    CrossRef  |  Direct Link  |  

  23. Ouwehand, A.C. and S.J. Salminen, 1998. The health effects of cultured milk products with viable and non-viable bacteria. Int. Dairy J., 81: 749-758.
    CrossRef  |  Direct Link  |  

  24. Ouwehand, A.C., P.V. Kirjavainen, C. Shortt and S. Salminen, 1999. Probiotics: Mechanics and established effects. Int. Dairy J., 9: 43-52.

  25. Ouwehand, A.C., S. Salminen and E. Isolauri, 2002. Probiotics: An overview of beneficial effects. Antonie Van Leeuwenhoek, 82: 279-289.
    PubMed  |  Direct Link  |  

  26. Patterson, J.A. and K.M. Burkholder, 2003. Application of prebiotics and probiotics in poultry production. Poult. Sci., 82: 627-631.
    CrossRef  |  PubMed  |  Direct Link  |  

  27. Strus, M., K. Pakosz, H. Gosciniak, A.P. Mordarska and E. Rozynek et al., 2001. Antagonistic activity of Lactobacillus strains against anaerobic gastrointestinal tract pathogens (Helicobacter pylori, Campylobacter coli, Campylobacter jejuni, Clostridium difficile). Med. Dosw Mikrobiol., 53: 133-142.
    PubMed  |  

  28. Sultana, K., G. Godward, N. Reynolds, R. Arumugaswamy, P. Peiris and K. Kailasaphathy, 2000. Encapsulation on probiotic bacteria with alginate-starch and evaluation of survival in simulated gastrointestinal condition and in yoghurt. Int. J. Food Microbiol., 62: 47-55.
    PubMed  |  

  29. Charteris, W.P., P.M. Kelly, L. Morelli and J.K. Collins, 1998. Development and application of an in vitro methodology to determine the transit tolerance of potentially probiotic Lactobacillus and Bifidobacterium species in the upper human gastrointestinal tract. J. Appl. Microbiol., 84: 759-768.
    CrossRef  |  PubMed  |  Direct Link  |  

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