Research Article

Transmission Electron Microscopy (TEM) Study of the Oocyst of Eimeria tenella

Irungbam Karuna Devi, Y. Geeta Devi, Kh. Victoria Chanu, M. Ayub Ali, Jully Gogoi Tewari, M. Prava, David Malsawmkima and Gautam Patra
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The present work describes isolation, cell wall preparation, morphological as well as molecular detection of oocyst of Eimeria tenella. Based on transmission electron microscopy, the oocyst of this species showed the outer and inner wall without veil forming bodies; cross section of sporozoites with different organelles and macrogamete structure at ultrastructural level. Polymerase Chain Reaction (PCR) based on Internal Transcribed Spacer 1 (ITSI) regions of ribosomal DNA (rDNA) demonstrated 270 bp specific for E. tenella. Results of this study provide the basis for further ultrastructural studies of the organism. The present study also suggested that morphological characteristics along with molecular identification are prerequisite for validation and identification of Eimeria species.

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Irungbam Karuna Devi, Y. Geeta Devi, Kh. Victoria Chanu, M. Ayub Ali, Jully Gogoi Tewari, M. Prava, David Malsawmkima and Gautam Patra, 2010. Transmission Electron Microscopy (TEM) Study of the Oocyst of Eimeria tenella. International Journal of Poultry Science, 9: 1050-1055.

DOI: 10.3923/ijps.2010.1050.1055



  1. Aikawa, M., M. Torii, A. Sjolander, K. Berzins, P. Perlmann and L.H. Miller, 1990. Pfl55/RESA antigen is localized in dense granules of PIasmodium falciparum, merozoites. Exp. Parasitol., 71: 326-329.
    CrossRef  |  

  2. Cai, J., M.D. Collins, V. McDonald and D.E. Thompson, 1992. PCR cloning and nucleotide sequence determination of the 18S rRNA genes and internal transcribed spacer 1 of protozoan parasites Cryptosporidium parvum and Cryptosporidium muris. Biochimica et Biophysica Acta, 1131: 317-320.
    CrossRef  |  

  3. Carruthers, V.B. and L.D. Sibley, 1997. Sequential protein secretion from three distinct organelles of Toxoplasma gondii accompanies invasion of human fibroblasts. Eur. J. Cell Biol., 73: 114-123.
    PubMed  |  Direct Link  |  

  4. Devereux, J., P. Haberli and O. Smithies, 1984. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acid Res., 12: 387-395.
    PubMed  |  Direct Link  |  

  5. Elwasila, M., 1984. Fine structure of the process of oocyst wall formation of Eimeria maxima (Apicomplexa: Eimeriina). Acta Vet. Hung., 32: 159-163.
    PubMed  |  Direct Link  |  

  6. Eschenbacher, K.H., P. Eggli, M. Wallach and R. Braun, 1996. Characterization of a 14 kDa oocyst wall protein of Eimeria tenella and E. acervulina. Parasitol., 112: 169-176.
    PubMed  |  Direct Link  |  

  7. Ferguson, D.J., S. Brecht and D. Soldati, 2000. The microneme protein MIC4, or an MIC4-like protein, is expressed within the macrogamete and associated with oocyst wall formation in Toxoplasma gondii. Int. J. Parasitol., 30: 1203-1209.
    PubMed  |  Direct Link  |  

  8. Ferguson, D.J., S.I. Belli, N.C. Smith and M.G. Wallach, 2003. The development of the macrogamete and oocyst wall in Eimeria maxima: Immuno-light and electronmicroscopy. Int. J. Parasitol., 33: 1329-1340.
    PubMed  |  Direct Link  |  

  9. Hemphill, A., N. Gajendran, S. Sonda, N. Fuchs, B. Gottstein, B. Hentrich and M. Jenkins, 1998. Identification and characterisation of a dense granule-associated protein in Neospora caninum tachyzoites. Int. J. Parasitol., 28: 429-438.
    PubMed  |  Direct Link  |  

  10. Holmdahl, O.J.M. and J.G. Mattsson, 1996. Rapid and sensitive indentification of Neospora caninum by in vitro amplification of the internal transcribed spacer1. Parasitol., 112: 177-182.
    PubMed  |  

  11. Leriche, M.A. and J.F. Dubremetz, 1991. Characterization of the protein contents of rhoptries and dense granules of Toxoplasma gondii tachyzoites by subcellular fractionation and monoclonal antibodies. Mol. Biochem. Parasitol., 45: 249-259.
    CrossRef  |  

  12. Levine, N.D., J.O. Corliss, F.E. Cox, G. Deroux and J. Grain et al., 1980. A newly revised classification of the protozoa. J. Eukaryotic Microbiol., 27: 37-58.
    CrossRef  |  Direct Link  |  

  13. Lindsay, D.S., 1999. A structural study of the Neospora caninum oocyst. Int. J. Parasitol., 29: 1521-1523.
    CrossRef  |  

  14. Patra, G., M.A. Ali , K.V. Chanu, L. Jonathan and L.K. Joy et al., 2010. PCR based diagnosis of Eimeria tenella infection in broiler chicken. Int. J. Poult. Sci., 9: 813-818.
    CrossRef  |  Direct Link  |  

  15. Reece, K.S., M.E. Siddal, E.M. Burreson and J.E. Graves, 1997. Phytogenetic analysis of Perkinsus based on actin gene sequences. J. Parasitol., 83: 417-423.
    PubMed  |  Direct Link  |  

  16. Rick, B., I.F. Dubremetz and R. Entzeroth, 1998. A merozoite-specific 22-kDa rhoptry protein of the coccidium Eimeria nieschulzi (Sporozoa, Coccidia) is exocytosed in the parasitophorous vacuole upon host cell invasion. Parasitol. Res., 84: 291-296.
    CrossRef  |  Direct Link  |  

  17. Ryley, J.F., 1973. Cytochemistry, Physiology and Biochemistry. In: The Coccidia: Eimeria, Isospora, Toxoplasma and Related Genera, Hammond, D.H. and P.L. Long (Eds.). University Park Press, Baltimore, London, pp: 145-181

  18. Belli, S.I., N.C. Smith and D.J.P. Ferguson, 2006. The coccidian oocyst: A tough nut to crack trends. Parasitology, 22: 416-423.
    CrossRef  |  

  19. Sambrook, J., E.F. Fritsch and T. Maniatis, 1989. Molecular Cloning: A Laboratory Mannual. 2nd Edn., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, ISBN-13: 9780879693091

  20. Rose, M.E., A.M. Lawn and B. Millard, 1984. The effect of immunity on the early eventsin the life-cycle of Eimeriu tenella in the cecal mucosa of the chicken. Parasitology, 88: 199-210.

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