Subscribe Now Subscribe Today
Research Article

Effect of Chlortetracycline Additive in Broilers Fed Local Diets on Antibody Titers to NDV Vaccine

Retno Murwani and Sri Murtin
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

A research was carried out to study the effect of antibiotic chlortetracycline in drinking water on broilers antibody titers to NDV vaccine. Broilers were fed local diet with two different sources of essential amino acid i.e. yeast (in diet 1) or mix lysine, methionine and choline (in diet 2). Two hundred and fifty day old chicks CP 707 were randomly assigned into five treatment diets i.e. 1) commercial diet (Dc), 2) diet 1 without chlortetracycline (D1), (3) diet 2 without chlortetracycline (D2), (4) diet 1 plus chlortetracycline 500 ppm via drinking water (D1+), (5) diet 2 plus chlortetracycline 500ppm via drinking water (D2+). Isoenergy diet and water were given ad libitum. NDV vaccine were given simultaneously via eyedrop and subcutaneous on day 4. Serum antibody titers were measured on day 18, 21 and 25 by Haemaglutination Inhibition Test and expressed as Geometric Mean Titer (log 2). Antibody titer to NDV vaccine was already detectable two weeks after vaccination (on day 18). The titer value was highest in D1+ reaching 6.0 which was above protective level and the lowest value was found in D1 i.e. 2.75 (p<0.05). On day 21 the highest titer was also found in D1+ which remained protective i.e. 5.5 and the lowest one in Dc. On day 25 the highest titer was found in D2 (6.5) and the lowest in Dc (4.25) (p<0.05). These results showed that antibody titers to NDV vaccine in broilers receiving local diet minus antibiotics can match the titers of broilers receiving local diet plus antibiotics. Moreover the broilers receiving local diet without antibiotics produced protective and better titers values at the end of sampling period on day 25 or 3 weeks after vaccination, reaching 5.0 in D1 and 6.5 in D2 respectively.

Related Articles in ASCI
Search in Google Scholar
View Citation
Report Citation

  How to cite this article:

Retno Murwani and Sri Murtin, 2009. Effect of Chlortetracycline Additive in Broilers Fed Local Diets on Antibody Titers to NDV Vaccine. International Journal of Poultry Science, 8: 755-759.

DOI: 10.3923/ijps.2009.755.759



1:  Chew, B.P. and J.S. Park, 2004. Carotenoid action on the immue response. J. Nutr., 134: 257-261.

2:  Cook, M.E., 2000. The interplay between modern management practices and the chicken How immune response and the physiological mechanisms for growth and efficiency have adapted over time. Where do we go from here? Proceeding Alltech`s 16th Annual Symposium, (AAS`00), Nottingham, UK., pp: 97-109

3:  Dibner, J.J. and J.D. Richards, 2005. Antibiotic growth promoters in agriculture: History and mode of action. Poult. Sci., 84: 634-643.
CrossRef  |  Direct Link  |  

4:  Ferket, P.R., 2004. Alternatives to antibiotics in poultry production: Responses, practical experience and recommendations. Proceedings of Alltech's 20th Annual Symposium on Nutritional Biotechnology in the Feed and Food Industries, May 22-26, 2004, Nottingham, UK., pp: 56-67

5:  Gao, J., H.J. Zhang, S.H. Yu, S.G. Wu and I. Yoon et al., 2008. Effects of yeast culture in broiler diets on performance and immunomodulatory functions. Poult. Sci., 87: 1377-1384.
CrossRef  |  PubMed  |  Direct Link  |  

6:  Hartadi, H., S. Reksohadiprodjo and A.D. Tillman, 1986. Feed Composition Table for Indonesia. Gadjah Mada University Press, Bulaksumur, Yogyakarta

7:  Hikosaka, K., M. El-Abasy, Y. Koayama, M. Motobu and K. Koge et al., 2006. Immunostimulating effects of the polyphenol-rich fraction of sugar cane (Saccharum officinarum L.) extract in chickens. Phytol. Res., 21: 120-125.

8:  Honjo, K., T. Hagiwara, K. Itoh, E. Takashasi and Y. Hirota, 1993. Immunohistochemical analysis of tissue distribution of B and T cells in germfree and conventional chickens. J. Vet. Med. Sci., 55: 1031-1034.
Direct Link  |  

9:  Hooge, D.M., M.D. Sims, A.E. Sefton, A. Connolly and P. Spring, 2003. Effect of dietary mannan oligosaccharide, with or without bacitracin or virginiamycin, on live performance of broiler chickens at relatively high stocking density on new litter. J. Applied Poult. Res., 12: 461-467.
Direct Link  |  

10:  Indonesian Directorate of Veterinary Society, 1999. Guide to Control of Infectious Disease. Directorate of Veterinary Society Indonesian Agriculture Department, Jakarta

11:  Kidd, M.T., 2004. Nutritional modulation of immune function in broilers. Poult. Sci., 83: 650-657.
CrossRef  |  Direct Link  |  

12:  Klasing, K.C., 1997. Interaction Between Nutrition and Infectious Disease. In: Diseases of Poultry, Calnek, B.W. (Ed.). Iowa State University Press, Ames, IA., USA., pp: 73-80

13:  Murwani, R. and B. Bayuardhi, 2007. Broilers serum cholesterol and glutamic oxaloacetic transaminase and their relation to antibiotic in feed and medication programs in four broiler producers in semarang region-central Java, Indonesia. Int. J. Poult. Sci., 6: 266-270.
CrossRef  |  Direct Link  |  

14:  Murwani, R., 2008. Effect of corn or sorghum in combination with soybean meal or mungbean as feed ingredients on the serum antibody titres to NDV vaccine in broiler chickens. Int. J. Poult. Sci., 7: 497-501.
CrossRef  |  Direct Link  |  

15:  Neyestani, T.R., 2008. Polyphenols and Immunity. In: Wild-Type Food in Health Promotion and Disease Prevention, De Meester, F. and R.R. Watson (Eds.). Humana Press Inc., Totowa, NJ., USA., ISBN: 978-1-58829-668-9, pp: 413-434
Direct Link  |  

16:  Nitsan, Z., G. Ben-Avraham, Z. Zoref and I. Nir, 1991. Growth and development of the digestive organs and some enzymes in broiler chicks after hatching. Br. Poult. Sci., 32: 515-523.
CrossRef  |  PubMed  |  Direct Link  |  

17:  Samarasinghe, K., C. Wenk, K.F.S.T. Silva and J.M.D.M. Gunasekera, 2003. Turmeric (Curcuma longa) root powder and mannanoligosaccharides as alternatives to antibiotics in broiler chicken diets. Asian-Aust. J. Anim. Sci., 16: 1495-1500.
Direct Link  |  

18:  Scalbert, A., I.T. Johnson and M. Saltmarsh, 2005. Polyphenols: Antioxidants and beyond. Am. J. Clin. Nutr., 81: 215S-217S.
CrossRef  |  PubMed  |  Direct Link  |  

19:  Spring, P., C. Wenk, K.A. Dawson and K.E. Newman, 2000. The effects of dietary mannaoligosaccharides on cecal parameters and the concentrations of enteric bacteria in the ceca of salmonella-challenged broiler chicks. Poult. Sci., 79: 205-211.
CrossRef  |  PubMed  |  Direct Link  |  

20:  Villegas, P., 1987. Laboratory Manual Avian Virus Disease (AM 805). University of Georgia, Athens, Georgia

21:  Yang, Y., P.A. Iji, A. Kocher, E. Thomson, L.L. Mikkelsen and M. Choct, 2008. Effects of mannanoligosaccharide in broiler chicken diets on growth performance, energy utilisation, nutrient digestibility and intestinal microflora. Br. Poult. Sci., 49: 186-194.
CrossRef  |  PubMed  |  Direct Link  |  

22:  Yang, Y., P.A. Iji and M. Choct, 2007. Effects of different dietary levels of mannanoligosaccharide on growth performance and gut development of broiler chickens. Asian-Australasian J. Anim. Sci., 7: 1084-1091.
Direct Link  |  

©  2022 Science Alert. All Rights Reserved