Subscribe Now Subscribe Today
Research Article
 

Evaluation of Commercial Disinfectants Against Bacterial Pathogens Isolated from Broiler Farms



Essam S. Soliman, Mohamed A.A. Sobeih, Z.H. Ahmad, M.M. Hussein and H.A.A. Moneim
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail
ABSTRACT

Five disinfectants [TH4® (combination of quaternary ammonium compounds and gluteraldhyde), Microzal® (combination of quaternary ammonium compounds and gluteraldhyde), Incospect IC 22XA (combination of quaternary ammonium compounds, gluteraldhyde and formalin), Povidone Iodine® (iodophore) and Formalin® (commercial formaldehyde 37%) were tested in a laboratory trials against four bacterial isolates (Staphylococcus aureus, Escherchia coli, Klebsiella oxytoca and Pseudomonas aureuginosa) at concentration of (~105) isolated during epidemiological surveillance. The trials were carried in the presence and absence of organic matter (dried yeast 5%) using MIC use-dilution test. Minutely samples were collected for the bacterial counts. In the absence of organic matter, TH4® achieved the 100% killing efficacy against Staphylococcus aureus, Escherchia coli, Klebsiella oxytoca and Pseudomonas aureuginosa after 5, 5, 10 and 5 min (p<0.0001) respectively, Microzal® and Incospect IC 22XA achieved the 100% killing efficacy against the four Micro-organisms after 5 min (p<0.0001), Povidone Iodine® achieved the 100% killing efficacy after 5, 5, 5 and 10 min (p<0.0001) respectively and Formalin® achieved the 100% killing efficacy after 5, 5, 5 and 20 min (p<0.0001) respectively. In the presence of organic matter, TH4® achieved the 100% killing efficacy against Staphylococcus aureus, Escherchia coli, Klebsiella oxytoca and Pseudomonas aureuginosa after 10, 5, 20 and 20 min (p<0.0001) respectively, Microzal® achieved the 100% killing efficacy after 5, 5, 5 and 10 min (p<0.0001) respectively, Incospect IC 22XA achieved the 100% killing efficacy after 5, 5, 10 and 5 min (p<0.0001) respectively, Povidone Iodine® achieved the 100% killing efficacy against the four micro-organisms after 30 min (p<0.0001) and Formalin® achieved the 100% killing efficacy after 30, 5, 20 and 20 min (p<0.0001) respectively. The results revealed that quaternary ammonium-gluteraldhyde combination (TH4®, Microzal® and Incospect IC 22XA) although they are not proven to be environmentally safe; they are the most powerful disinfectants because of the synergistic action of the quaternary ammonium and gluteraldhyde bases.

Services
Related Articles in ASCI
Similar Articles in this Journal
Search in Google Scholar
View Citation
Report Citation

 
  How to cite this article:

Essam S. Soliman, Mohamed A.A. Sobeih, Z.H. Ahmad, M.M. Hussein and H.A.A. Moneim, 2009. Evaluation of Commercial Disinfectants Against Bacterial Pathogens Isolated from Broiler Farms. International Journal of Poultry Science, 8: 728-732.

DOI: 10.3923/ijps.2009.728.732

URL: https://scialert.net/abstract/?doi=ijps.2009.728.732

REFERENCES
1:  Bloomfield, S.F., M. Arther, E. Looney, K. Begun and H. Patel, 1991. Comparative testing of disinfectant and antiseptic products using proposed European suspension testing methods. Lett. Applied Microbiol., 13: 233-237.
CrossRef  |  Direct Link  |  

2:  Cruickshank, R., J.P. Daguid, B.P. Marnion and R.H.A. Swain, 1980. Medical Microbiology. 12th Edn., Churchill Livingstone, Edinburgh, London, pp: 180-188.

3:  Denyer, S.P. and G.S.A.B. Stewart, 1998. Mechanisms of action of disinfectants. Int. Biodeterior. Biodegrad., 41: 261-268.
CrossRef  |  Direct Link  |  

4:  Herigstad, B., M. Hamilton and J. Heersink, 2001. How to optimize the drop plate method for enumerating bacteria. J. Microbiol. Methods, 44: 121-129.
Direct Link  |  

5:  Maillard, J.Y., 2002. Bacterial target sites for biocide action. Symp. Ser. Soc. Applied Microbiol., 92: 16S-27S.
PubMed  |  Direct Link  |  

6:  Mosteller, T.M. and J.R. Bishop, 1993. Sanitizer efficacy against attached bacteria in a milk biofilm. Food Prot., 56: 34-41.
CrossRef  |  Direct Link  |  

7:  Parkinson, E., 1981. Testing of Disinfectants for Veterinary and Agricultural Use in Disinfectants: Their Use and Evaluation of Effectiveness. Academic Press, London, UK., ISBN: 0121813800, pp: 33-36.

8:  Pepper, I.L., K.L. Josephson, R.L. Bailey, M.D. Burr and C.P. Gerba, 1993. Survival of indicator organisms in sonoran dessert soil amended with sewage sludge. J. Environ. Sci. Health, 28: 1287-1302.
CrossRef  |  Direct Link  |  

9:  Robinson, R.A., H.L. Bodily, D.F. Robinson and R.P. Christensen, 1988. A suspension methods to determine reuse life of chemical disinfectants during clinical use. Applied Environ. Microbiol., 54: 158-164.
Direct Link  |  

10:  Rudolfs, W., L.L. Falk and R.A. Rgotzki, 1950. Literature review of the occurrence and survival of enteric pathogenic and relative organisms in soil, water sewage and sludge and on vegetation: I. Bacterial and virus diseases. Sewage Ind. Wastes, 22: 1261-1281.
Direct Link  |  

11:  Sainsbury, D., 1982. Poultry Health and Management. Granada, London, pp: 168.

12:  Zelver, N., M. Hamilton, B. Pitts, D. Goeres, D. Walker, P. Sturman and J. Heersink, 1999. Measuring Antimicrobial Effects on Biofilm Bacteria. In: Biofilm: Methods in Enzymology, Doyle, R.J. (Ed.). Academic Press, San Diego, CA., USA., pp: 608-628.

©  2021 Science Alert. All Rights Reserved