Paulo Reis de Carvalho
Department of Clinical Medicine, Faculty of Veterinary Medicine and Animal Science, University of S�o Paulo, Orlando Marques de Paiva, no 87, CEP 05508-270, S�o Paulo, SP, Brazil
Maria Carolina Goncalves Pita
Department of Clinical Medicine, Faculty of Veterinary Medicine and Animal Science, University of S�o Paulo, Orlando Marques de Paiva, no 87, CEP 05508-270, S�o Paulo, SP, Brazil
Eduardo Piber Neto
Department of Clinical Medicine, Faculty of Veterinary Medicine and Animal Science, University of S�o Paulo, Orlando Marques de Paiva, no 87, CEP 05508-270, S�o Paulo, SP, Brazil
Cassio Xavier de Mendonca Jr.
Department of Clinical Medicine, Faculty of Veterinary Medicine and Animal Science, University of S�o Paulo, Orlando Marques de Paiva, no 87, CEP 05508-270, S�o Paulo, SP, Brazil
ABSTRACT
Two hundred eighty-eight 32-wk-old Hisex White laying hens were used in this research during a 10 week period, arranged in a 2 x 5 completely randomized factorial design, with three replicates of eight birds per treatment. Two groups: fish oil (OP) and Marine Algae (AM) with five DHA levels (120, 180, 240, 300 and 360 mg/100 g diet) were assigned including two control groups, birds fed corn and soybean basal diet (CON) and a diet supplemented with AM (AM420) to study the efficiency of egg yolk fatty acid enrichment. The Arachidonic Acid (AA), Linoleic (LA) and PUFAs n-6 mean values ranged, respectively, 98.71 mg, 987.70 mg and 1108.92 mg/yolk for the hens fed the CON diet and 38.87 mg, 734.22 mg and 802.79 mg/yolk for the OP360 group. The percentage of AA incorporation (% INC) in egg yolk decreased linearly with the increase of DHA levels in the diets supplemented with OP and AM, from 4.81% (CON) to 2.57% (OP360) and 3.51% (AM420). The efficiency (%) of DHA incorporation into the yolk decreased linearly with increasing of DHA levels in the diet supplemented with OP and AM, from 85.11% (OP120) and 65.28% (AM120) to 49.45% (OP360) and 34.06% (AM420). The levels of DHA in the egg yolk of birds supplemented with OP had significant increase from 22.64 mg/yolk (CON) to 187.91 mg/yolk in OP360 group. PUFAs n-3 means into the eggs were significantly (p<0.05) increased when the groups CON (62.16 mg/yolk) and OP360 (218.62 mg/yolk) were compared.
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How to cite this article
Paulo Reis de Carvalho, Maria Carolina Goncalves Pita, Eduardo Piber Neto and Cassio Xavier de Mendonca Jr., 2009. Efficiency of PUFAs Incorporation from Marine Sources in Yolk Egg`s Laying
Hens. International Journal of Poultry Science, 8: 603-614.
DOI: 10.3923/ijps.2009.603.614
URL: https://scialert.net/abstract/?doi=ijps.2009.603.614
DOI: 10.3923/ijps.2009.603.614
URL: https://scialert.net/abstract/?doi=ijps.2009.603.614
REFERENCES
- Abril, J.R. and W. Barclay, 1998. Production of docosahexaenoic acid-enriched poultry eggs and meat using an algae-based feed ingredient. World Rev. Nutr. Diet, 83: 77-88.
CrossRefDirect Link - Barclay, W.R., K.M. Meager and J.R. Abril, 1994. Heretrophic production of long chain omega-3 fatty acids utilizings algae and algae-like microorganisms. J. Applied Phycol., 6: 123-129.
CrossRefDirect Link - Bligh, E.G. and W.J. Dyer, 1959. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol., 37: 911-917.
CrossRefPubMedDirect Link - Chen, P.H., R.H. Common, N. Nikolaiczuk and H.F. Macrae, 1965. Some effects of added dietary fats on the lipid composition of hen's egg yolk. J. Food Sci., 30: 838-845.
CrossRefDirect Link - Cherian, G. and J.S. Sim, 2003. Maternal and posthatch dietary polyunsaturated fatty acids alter tissue tocopherol status of chicks. Poult. Sci., 82: 681-686.
Direct Link - Cherian, G., F.W. Wolfe and J.S. Sim, 1996. Feeding dietary oils with tocopherols: Effects on internal qualities of eggs during storage. J. Food Sci., 61: 15-18.
CrossRef - Folch, J., M. Lees and G.H.S. Stanley, 1957. A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem., 226: 497-509.
CrossRefPubMedDirect Link - Galobart, J., A.C. Barroeta, L. Cortinas, M.D. Baucells and R. Codony, 2002. Accumulation of α-tocopherol in eggs enriched with omega-3 and omega-6 polyunsaturated fatty acids. Poult. Sci., 81: 1873-1876.
Direct Link - Guardiola, F., R. Codony, M. Rafecas, J. Boatella and A. Lopez, 1994. Fatty acid composition and nutritional value of fresh eggs from large-and small-scale farms. J. Food Compos. Anal., 7: 171-188.
Direct Link - Hargis, P.S., M.E. van Elswyk and B.M. Hargis, 1991. Dietary modification of yolk lipid with menhaden oil. Poult. Sci., 70: 874-883.
PubMed - Hartman, L. and R.C.A. Lago, 1973. Rapid preparation of fatty acid methyl esters from lipids. Lab. Pract., 22: 475-476.
PubMedDirect Link - Herber, S.M. and M.E. van Elswyk, 1996. Dietary marine algae promotes efficient deposition of n-3 fatty acids for the production of enriched shell eggs. Poult. Sci., 75: 1501-1507.
PubMedDirect Link - Hermier, D., 1997. Lipoprotein metabolism and fattening in poultry. J. Nutr., 127: S805-S808.
Direct Link - Hwang, D., 1989. Essential fatty acids and immune response. FASEB J., 3: 2052-2061.
PubMedDirect Link - Jiang, Z.R., D.U. Ahn and J.S. Sim, 1991. Effects of feeding flax and two types of sunflower seeds on fatty acid compositions of yolk lipid classes. Poult. Sci., 70: 2467-2475.
PubMed - Jiang, Z. and J.S. Sim, 1993. Consumption of n-3 polyunsaturated fatty acid enriched eggs and changes in plasma lipids of human subjects. Nutrition, 9: 513-518.
PubMedDirect Link - Leskanich, C.O. and R.C. Noble, 1997. Manipulation of the n-3 polyunsaturated fatty acid composition of avian eggs and meat. World's Poult. Sci. J., 53: 155-183.
CrossRefDirect Link - Marshall, A.C., A.R. Sams and M.E. van Elswyk, 1994. Oxidative stability and sensory quality of stored eggs from hens fed 1,5% menhaden oil. J. Food Sci., 59: 561-563.
Direct Link - Naber, E.C. and M.D. Biggert, 1989. Patterns of lipogenesis in laying hens fed a high fat diet containing safflower oil. J. Nutr., 119: 690-695.
Direct Link - Neuringer, M., W.E. Connor, C.V. Petten and L. Barstad, 1984. Dietary omega-3 fatty acid daficiencey and visual loss in infant rhesus monkeys. J. Clin. Invest., 73: 272-276.
CrossRefDirect Link - Nielsen, H., 1998. Hen age and fatty acid composition of egg yolk lipid. Br. Poult. Sci., 39: 53-56.
CrossRefDirect Link - Scheuermann, G.N. and C. Bellaver, 1995. Estado da arte e a perspectiva para pesquisa futura em nutricao de aves. Reuniao Anual Da Sociedade Brasileira De Zootecnia, 32. Anais. Brasilia, DF., pp: 465-473. http://orton.catie.ac.cr/cgi-bin/wxis.exe/?IsisScript=ACERVO.xis&method=post&formato=2&cantidad=1&expresion=mfn=028519.
- Sim, J.S., 1998. Designer eggs and their nutritional and functional significance. World Rev. Nutr. Diet, 83: 89-101.
Direct Link - Simopoulos, A.P., B. Koletzko, R.E. Anderson, G. Hornstra and R.P. Mensink et al., 1994. The 1st congress of the international society for the study of fatty acids and lipids (ISSFAL): fatty acids and lipids from cell biology to human disease. J. Lipid Res., 35: 169-173.
PubMedDirect Link - Simopoulos, A.P., A. Leaf and N. Salem Jr., 1999. Workshop on the essentiality of and recommended dietary intakes for omega-6 and omega-3 fatty acids. J. Am. College Nutr., 18: 487-489.
CrossRefPubMedDirect Link - Snedecor, G.W. and W.G. Cochran, 1967. Statistical Methods. 6th Edn., Oxford and IBH Publishing Co. Pvt. Ltd., New Delhi, India, Pages: 593.
Direct Link - Stadelman, W.J., 1995. Quality Identification of Shell Eggs. In: Egg Science and Technology, Srtadelman, W.J. and O.J. Cotterill (Eds.). Food Product Press, Binghamton, NY., pp: 39-80.
Direct Link - Surai, P.F. and N.H.C. Sparks, 2001. Designer eggs: From improvement of egg composition to functional food. Trends Food Sci. Technol., 12: 7-16.
CrossRefDirect Link - Tsuzuki, E.T., E.R. Garcia, A.E. Murakami and J.R. Galli, 2003. Utilization of sunflower seed in laying hen rations. Rev. Bras. Cienc. Avic., 5: 179-182.
CrossRefDirect Link - Van Elswyk, M.E., 1993. Designer foods: Manipulating the fatty acid composition of meat and eggs for the health conscious consumer. Nutr. Today, 28: 21-28.
Direct Link - Van Elswyk, M.E., 1997. Comparison of n-3 fatty acid sources in laying hen rations for improvement of whole egg nutritional quality: A review. Br. J. Nutr., 78: S61-S69.
Direct Link - Von Schacky, C. and J. Dyerberg, 2001. Omega 3 fatty acids from eskimos to clinical cardiology-what took us so long? World Rev. Nutr. Diet, 88: 90-99.
Direct Link - Watkins, B.A. and R.G. Elkin, 1992. Dietary modulation of oleic and stearic acids in egg yolk. J. Food Comp. Anal., 5: 209-215.
Direct Link - Watkins, B.A., Y. Li, H.E. Lippman and M.F. Seifert, 2001. Omega-3 polyunsaturated fatty acids and skeletal health. Exp. Biol. Med., 226: 485-497.
Direct Link - Watkins, B.A., Y. Li and M.F. Seifert, 2001. Nutraceutical fatty acids as biochemical and molecular modulators of skeletal biology. J. Am. Coll. Nutr., 20: 410S-416S.
Direct Link - Wheeler, P., D.W. Peterson and G.D. Michaels, 1959. Fatty acid distribution in egg yolk as influenced by type and level of dietary fat. J. Nutr., 69: 253-260.
Direct Link - Zeidler, G., 1998. Eggs vital to human and animal medicine. Misset World Poult., 14: 33-34.
Direct Link