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Research Article
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Antiinflammatory and Analgesic Effects of Phlomis lanceolata Boiss.
and Hohen. Extracts and Examination of their Components |
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M. Mohajer ,
P. Sarkhail ,
N. Hajarolasvadi ,
M.J. Zamani ,
R. Khorasani ,
A. Shafiee ,
G. Amin
and
M. Abdollahi
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ABSTRACT
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The purpose of this investigation was to study the anti-inflammatory and
analgesic properties of total extract and four fractions (ether, ethyl acetate,
n-butanol and water) from Phlomis lanceolata (Lamiaceae) in mice.
The plant material was extracted with methanol. In order to estimate the
polarity of the active compounds, the total extract was dissolved in water and
the water soluble portion was successively partitioned between ether, ethyl
acetate and n-buthanol. The total extract and four fractions were
analyzed by Thin Layer Chromatography (TLC) by use of specific reagents. Dose of
100 mg kg 1 of each extracts were used in carrageenan-induced paw
edema, formalin and writhing nociception tests in mice. All compounds reduced
paw edema in comparison to the control group at 1, 3, 5 and 7 h post carrageenan
injection. The total, ether and aqueous extracts were similar to indomethacin
while the ethyl acetate extract was weaker than indomethacin in reduction of paw
edema. All extracts induced antinociception in both phases of formalin test. The
total and ether extracts were as potent as indomethacin in both phases of
formalin test. The ethyl acetate extract was weaker than indomethacin in the
second phase of formalin-test while the n-butanol and aqueous extracts
showed more antinociception than indomethacin in the second phase of formalin
test. All extracts as well as indomethacin induced antinociception in writhing
test in comparison to control. The total and aqueous extracts induced the same
antinociception as indomethacin while ether, ethyl acetate and n-butanol
showed weaker antinociception than indomethacin. Positive results for iridoids
and phenolic compounds were indicated by phytochemical analysis of total
extract. Phenolic compounds were found in four fractions whereas only n-butanol
and aqueous fractions showed positive results for iridoid glycosides. The higher
antinociceptive effects of n-butanol and aqueous extracts in the
inflammatory phase of formalin test among different extracts tested, might back
to the presence of iridoid glycosides, phenolic glycosides or other glycosides.
These data suggest that different extracts of P. lanceolata produce
different antinociceptive activities that could be due to the effect of one or a
combination of the bioactive components in each extract.
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How
to cite this article:
M. Mohajer , P. Sarkhail , N. Hajarolasvadi , M.J. Zamani , R. Khorasani , A. Shafiee , G. Amin and M. Abdollahi , 2006. Antiinflammatory and Analgesic Effects of Phlomis lanceolata Boiss.
and Hohen. Extracts and Examination of their Components. International Journal of Pharmacology, 2: 50-54.
DOI: 10.3923/ijp.2006.50.54
URL: https://scialert.net/abstract/?doi=ijp.2006.50.54
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