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Research Article

Antiinflammatory and Analgesic Effects of Phlomis lanceolata Boiss. and Hohen. Extracts and Examination of their Components

M. Mohajer , P. Sarkhail , N. Hajarolasvadi , M.J. Zamani , R. Khorasani , A. Shafiee , G. Amin and M. Abdollahi
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The purpose of this investigation was to study the anti-inflammatory and analgesic properties of total extract and four fractions (ether, ethyl acetate, n-butanol and water) from Phlomis lanceolata (Lamiaceae) in mice. The plant material was extracted with methanol. In order to estimate the polarity of the active compounds, the total extract was dissolved in water and the water soluble portion was successively partitioned between ether, ethyl acetate and n-buthanol. The total extract and four fractions were analyzed by Thin Layer Chromatography (TLC) by use of specific reagents. Dose of 100 mg kg 1 of each extracts were used in carrageenan-induced paw edema, formalin and writhing nociception tests in mice. All compounds reduced paw edema in comparison to the control group at 1, 3, 5 and 7 h post carrageenan injection. The total, ether and aqueous extracts were similar to indomethacin while the ethyl acetate extract was weaker than indomethacin in reduction of paw edema. All extracts induced antinociception in both phases of formalin test. The total and ether extracts were as potent as indomethacin in both phases of formalin test. The ethyl acetate extract was weaker than indomethacin in the second phase of formalin-test while the n-butanol and aqueous extracts showed more antinociception than indomethacin in the second phase of formalin test. All extracts as well as indomethacin induced antinociception in writhing test in comparison to control. The total and aqueous extracts induced the same antinociception as indomethacin while ether, ethyl acetate and n-butanol showed weaker antinociception than indomethacin. Positive results for iridoids and phenolic compounds were indicated by phytochemical analysis of total extract. Phenolic compounds were found in four fractions whereas only n-butanol and aqueous fractions showed positive results for iridoid glycosides. The higher antinociceptive effects of n-butanol and aqueous extracts in the inflammatory phase of formalin test among different extracts tested, might back to the presence of iridoid glycosides, phenolic glycosides or other glycosides. These data suggest that different extracts of P. lanceolata produce different antinociceptive activities that could be due to the effect of one or a combination of the bioactive components in each extract.

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  How to cite this article:

M. Mohajer , P. Sarkhail , N. Hajarolasvadi , M.J. Zamani , R. Khorasani , A. Shafiee , G. Amin and M. Abdollahi , 2006. Antiinflammatory and Analgesic Effects of Phlomis lanceolata Boiss. and Hohen. Extracts and Examination of their Components. International Journal of Pharmacology, 2: 50-54.

DOI: 10.3923/ijp.2006.50.54



1:  Blumenthal, M., 2000. Herbal Medicines. Integrative Medicine Communications, Austin, pp: 419-423

2:  Kuhn, M.A. and D. Winston, 2000. Herbal Therapy and Supplements a Scientific and Traditional Approach. Lippincott, New York, pp: 2-15

3:  Golshani, S., F. Karamkhani, H.R. Monsef-Esfehani and M. Abdollahi, 2004. Antinociceptive effects of the essential oil of dracocephalum kotschyi in the mouse writhing test. J. Pharm. Pharm. Sci., 7: 77-80.
Direct Link  |  

4:  Monsef-Esfahani, H.R., A. Ghobadi, M. Iranshahi and A. Mohammad, 2004. Antinociceptive effect of Peganum harmala L. alkaloid on mouse formalin test. J. Pharm. Pharm. Sci., 7: 70-75.

5:  Rechinger, K.H., 1982. Flora iranica graz Austria. Acad. Druck Verlagsanstalt, 150: 292-313.

6:  Saracoglu, I., K. Kojima, U.S. Harput and Y. Ogihara, 1998. A new phenylethanoid glycoside from Phlomis pungens Willd. var pungens. Chem. Pharm. Bull., 46: 726-727.
Direct Link  |  

7:  Bucar, F., S. Ninov, I. Ionkova, T. Kartnig, M. Schubert-Zsilavecz, I. Asenov and B. Konuklugil, 1998. Flavonoids from Phlomis nissoli. Phytochemistry, 48: 573-575.
CrossRef  |  Direct Link  |  

8:  Zhang, C., C. Li and S.I. Feng, 1991. Iridoid glycosides from Phlomis rotate. Phytochemistry, 30: 4156-4158.

9:  Saracoglu, I., M. Inoue, I. Calis and Y. Ogihara, 1995. Studies on constituent with cytotoxic and cytostatic and activity of two Turkish medicinal plants Phlomis armenica and Scutellaria salviflora. Biol. Pharm. Bull., 18: 1396-1400.
Direct Link  |  

10:  Kyriakopoulo, I., P. Magiatis, A. Skaltounis, A. Nektarios and H. Catherine, 2001. Samioside, a new phenylethanoid glycoside with free-radical scavenging and antimicrobial activities from Phlomis. J. Natural Prod., 64: 1095-1097.
CrossRef  |  Direct Link  |  

11:  Couladis, M., A. Tanimanidis, O. Tzakou, I.B. Chinou and C. Harvala, 2000. Essential oil of Phlomis lanata growing in Greece: Chemical composition and antimicrobial activity. Planta Med., 66: 670-672.
Direct Link  |  

12:  Sarkhail, P., M. Abdollahi and A. Shafiee, 2003. Antinociceptive effect of Phlomis olivieri Benth., Phlomis anisodonta Boiss. and Phlomis persica Boiss. total extracts. Pharmacol. Res., 48: 263-266.
CrossRef  |  

13:  Gurbuz, I., O. Austan, E. Yesilada, E. Sozik and O. Kutsal, 2003. Anti-ulcerogenic activity of some plants used folk remedy in Turkey. J. Ethnopharmacol., 88: 93-97.
Direct Link  |  

14:  Evans, W.C., 2002. Trease and Evans Pharmacognosy. 15th Edn., W.B. Saunders, London, pp: 246-248

15:  Ismailoglu, U.B., I. Saracoglu, U.S. Harput and I. Sahin-Erdemli, 2002. Effects of phenlpropanoids iridoid glycosides on free radical-induced impairment of endothelium-dependent relaxation in rat aortic rings. J. Ethnopharmacol., 79: 193-197.
Direct Link  |  

16:  Kamel, M.S., K.M. Mohamed, H.A. Hassanean O. Kazuhiro, K. Ryoji and Y. Kazuo, 2000. Iridoid and megastigmane glycosides from Phlomis aurea. J. Ethnopharmacol., 55: 353-357.
CrossRef  |  Direct Link  |  

17:  Wagner, H. and S. Baldt, 1995. Plant Drug Analysis a Thin Layer Chromatography Atlas. 2nd Edn., Springer, Berlin, Heidelberg, pp: 53-362

18:  Harborn, J.B., 1998. Phytochmical Methods a Guide to Modern Techniques of Plant Analysis. 3rd Edn., Chapman and Hall, London, pp: 1-249.

19:  Kozak, W., I. Archuleta, K.P. Mayfield, A. Kozak, K. Rudolph and M.J. Kluger, 1998. Inhibitors of alternative pathways of arachidonate metabolism differentially affect fever in mice. Am. J. Physiol., 275: 1031-1040.
Direct Link  |  

20:  Alvarez, M.E., A.E. Rotelli and L.E. Pelzer, 2000. Phytochemical study and anti-inflammatory properties of Lampaya hieronymi schum ex Moldenke. Farmaco, 55: 502-505.

21:  Abdollahi, M., L. Jalali and M.H. Salehi-Surmaghi, 2002. Antinociceptive effects of Achillea santolina L. on mouse formalin test. Biomed. Res., 13: 49-52.

22:  Koster, R., M. Anderson and E.J. de Beer, 1959. Acetic acid-induced analgesic screening. Fed. Proc., 18: 412-412.
Direct Link  |  

23:  Jain, S.C., R. Jain, R.A. Sharma and F. Capasso, 1997. Pharmacological investigation of Cassiaitalica. J. Ethnopharmacol., 58: 135-142.
Direct Link  |  

24:  Vogel, H.G. and W.H. Vogel, 1997. Drug Discovery and Evaluation. 1st Edn., Springer-Verlag, Berlin, Heidelberg, New York pp: 376- 377

25:  Hwang, S., M. Lam, C. Li and T. Shen, 1996. Release of platelet activating factor and its involvement in the first phase of carrageenin rat foot edema. Eur. J. Pharmacol., 120: 33-41.

26:  Shafiee, A., M. Bagheri, M. Shekarchi and M. Abdollahi, 2003. The antinociceptive activities of 1-(4-aryl-2-thiazolyl)-3,5-disubstituted-2 pyrazolines in mouse writhing test. J. Pharm. Pharm. Sci., 6: 360-362.
PubMed  |  Direct Link  |  

27:  Rang, H.P., M.M. Dale and J.M. Ritter, 1999. Pharmacology. Churchill Livingston, New York, pp: 229-231

28:  Sahpaz, S., N. Garbacki, M. Tits and F. Bailleul, 2002. Isolation and pharmacological activity of phenylpropanoid esters from Marrubium vulgare. J. Enthopharmacol., 79: 393-402.
Direct Link  |  

29:  Recio, M.C., R.M. Giner, S. Manez and J.L. Rios, 1994. Structural considerations on the iridoid, as anti-inflammatorty agents. Planta Med., 60: 232-234.
Direct Link  |  

30:  Okayama, E., S. Fujimori, M. Yamazaki and T. Deyama, 1998. Pharmacologically active components of Viticis fructus (Vitex rotundifolia). II. The components having analgesic effects. Chem. Pharmaceut. Bull., 46: 655-662.
PubMed  |  Direct Link  |  

31:  Suksamran, A., S. Kumpun, K. Kirtikara, B. Yingyongnarongkul and S. Suksamrarn, 2002. Iridoids with anti-inflammatory activity from Vitex peduncularis. Planta Med., 68: 72-73.
PubMed  |  Direct Link  |  

32:  Raso, G.M., R. Meli, G. di Carlo, M. Pacilio and R. di Carlo, 2001. Inhibition of inducible nitric oxide synthase and cyclooxygenase-2 expression by flavonoids in macrophage J774A.1. Life Sci., 68: 921-931.
CrossRef  |  Direct Link  |  

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