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Research Article

Acute and Subchronic (28-day) Oral Toxicity Studies of the Aqueous Root Extract of Securidaca longepedunculata Fresen (Polygalaceae) in Mice

E.U. Etuk , R.A. Adebiyi , A.T. Elsa and B.M. Agaie
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The acute and subchronic (repeat dose) oral toxicity of aqueous root extract of Securidaca longepedunculata was evaluated in mice. In the acute toxicity test, oral administration of limit dose of 5 g kg-1 of the extract produced neither mortality nor any acute signs of toxicity throughout the 14 days period of observation. In the subchronic toxicity study, there was no mortality recorded among the animals when varying doses of the extract (300, 900 and 2700 mg kg-1 body weight) were administered orally for 28 days consecutively. The weekly body and organ weights of the mice showed no significant differences between the control and the mice treated with the extract except on the last week (day 28) where there was significant increase (p<0.05) in the body weight of mice treated with 2,700 mg kg-1 of the extract. In the haematological analysis, there were significant (p<0.05) decrease in the Red Blood Cell (RBC) count at 2700 mg kg-1 and in Packed Cell Volume (PCV) of mice treated with 300 and 2,700 mg kg-1 of the extract, while the differential leucocytes count showed no differences in any of the parameter examined. Also, there was no significant changes in the biochemical parameters tested. There was neither any gross lesion nor histopathological changes observed in the organs (liver, kidney and brain) examined. These findings suggest that the aqueous root extract of S. longepedunculata could be relatively safe when administered orally in mice.

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  How to cite this article:

E.U. Etuk , R.A. Adebiyi , A.T. Elsa and B.M. Agaie , 2006. Acute and Subchronic (28-day) Oral Toxicity Studies of the Aqueous Root Extract of Securidaca longepedunculata Fresen (Polygalaceae) in Mice. International Journal of Pharmacology, 2: 421-425.

DOI: 10.3923/ijp.2006.421.425


1:  Atawodi, S.E., T. Bulus, S. Ibrahim, D.A. Ameh, A.J. Nok, M. Mamman and M. Galadima, 2003. In vitro trypanocidal effect of methanolic extract of some Nigerian savannah plants. Afr. J. Biotechol., 2: 317-321.
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2:  Ekpendu, T.O., P. Anyago, D. Itywgh and F. Akpan, 2000. Nigerian ethnomedicine and medicinal plant flora: The Benue experience-Part 3. Nig. J. Nat. Prod. Med., 4: 13-22.

3:  Gad, S.C. and C.P. Chengelis, 1988. Acute Toxicology: Principles and Methods. Telford Press, Caldwell, NJ.

4:  Logney, G., M. Michael, S. Jogo and H. Eric, 2000. The occurrence of 2 hydroxyl-6-methioxy benzoic acid methyl ester in secundaca longepedunculata (fresen) root bark. Biotechnol. Agron. Social Environ., 4: 107-110.

5:  Interagency Research Animal Committee (IRAC), 2004. Recommendation on LD50 testing. http://dacu.odnih. gov/ARAC/iracld50html/11-30-04.

6:  Neuwinger, H.D., 1964. Afrikenische Arzeipflanzen and Jagdgifte. Wisenschaft liche Verlagesells, Stuttgart.

7:  OECD (testing guideline, 407), 1995. Repeat dose 28 days oral toxicity study in rodents.

8:  OECD Principles on Good Laboratory Practice, 2001. Handbook, good laboratory practice (GLP). Quality Practices for Regulated non Clinical Research and Development.

9:  Ojewole, J.A.O., O.R.S. Ilesanmi and G. Olayiwola, 2000. Pharmacology of Africa medicinal plants: Neuromuscular and cardiovascular properties of Securidaca longepedunculata. Nig. J. Natl. Prod. Med., 4: 30-36.

10:  Organization for Economic Cooperation and Development (OECD) Guidelines 425, 2000. Guideline document on acute oral toxicity. Environmental Health and Safety Monograph Series on Testing and Assessment No. 24.

11:  Van Wyk, B.E., B. van Quadshoom and N. Gericke, 1997. Medicinal Plants of South Africa. 1st Edn., Briza Publication, Pretoria, South Africa, pp: 236.

12:  Watt, J.M. and M.G. Breyer-Brandwijk, 1962. The Medicinal and Poisons Plants of Southern and Eastern Africa. 2nd Edn., Living Stone, London.

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