Subscribe Now Subscribe Today
Abstract
Fulltext PDF
References
Research Article
 
Effects of Oral Administration of Water Extract of Nigella sativa on the Hypothalamus Pituitary Adrenal Axis in Experimental Diabetes



Kamal M.S. Mansi
 
ABSTRACT

The present study was designed to evaluate the role of water extract of Nigella sativa on the hypothalamus-pituitary-adrenal axis in alloxan-induced diabetic rats. Forty male white rats were divided into four experimental groups control, diabetic. N. sativa-treated and N. sativa-treated diabetic. At the end of the experimental period (3 weeks), animals in all three groups were fasted for 12 h and blood samples were taken for the determination of glucose levels, serum concentrations of insulin, glucagon, corticosterone and Adrenocorticotropic hormone (ACTH ) in four groups. It was found that water extract of Nigella sativa was investigated for hypoglycemic effect in diabetic rats and induced significant reduction in serum glucose from (19.83±1.25 Mmol L-1) in diabetic group to (9.7±1.10 Mmol L-1) in N. sativa- treated diabetic group. However the blood glucose still higher than the control and N. sativa- treated group, serum insulin increased from (0.54±0.22 Mu L-1) in control group to (0.65±0.06 Mu L-1) in N. sativa –treated group and still higher than control in N. sativa –treated diabetic (0.58±0,06 Mu L-1), serum corticosterone increased in diabetic group (580 ± 22.36 nmol L-1 )compared to control group (311±18.42 nmol L-1) and decreased in N. sativa-treated (238±16.53 nmol L-1) and in N. sativa treated diabetic group (378±19.65 nmol L-1) and still higher than control. Serum Adrenocorticotropic hormone (ACTH) increased in diabetic group (20.72±2.42 pmol L-1 ) compared to control group (13.82±1.83 pmol L-1) and still lower in N. sativa -treated (10.64±13 pmol L-1) and in N. sativa treated diabetic group (15.42±1.18 pmol L-1) compared to diabetic group. The results suggest the beneficial role of N. sativa as hypoglycemic agents and as a protective effect against pancreatic ß-cells damage from alloxan induced diabetes in rats by decreasing oxidative stress and preserving pancreatic ß -cells integrity and also suggest that the antidiabetic effect of N. sativa may be attributed to increased glucose metabolism by increasing the serum concentration of insulin and inhibited the hypothalamus-pituitary-adrenal axis.

Services
Related Articles in ASCI
Similar Articles in this Journal
Search in Google Scholar
View Citation
Report Citation

 
  How to cite this article:

Kamal M.S. Mansi , 2006. Effects of Oral Administration of Water Extract of Nigella sativa on the Hypothalamus Pituitary Adrenal Axis in Experimental Diabetes. International Journal of Pharmacology, 2: 104-109.

DOI: 10.3923/ijp.2006.104.109

URL: https://scialert.net/abstract/?doi=ijp.2006.104.109

REFERENCES
Abdel, M.A., M. El Feki and E. Saleh, 1998. Effect of Nigella sativa, fish oil and localized on alloxan diabetic rats. Bioch. histopath studies. J. Egypt. Ger. Soc. Zool., 23: 237-265.

Agel, M. and R. Shaheen, 1996. Effects of the volatile oil of Nigella sativa seeds on the uterine smooth muscle of rat and guinea pig. J. Ethnopharmacol., 52: 23-26.
CrossRef  |  Direct Link  |  

Ahmad, F., P.M.M. Khalid, M. Khan, A.K. Chaubey, Rastogi and J.R. Kidwai, 1995. Hypoglycemic activity of pterocarpus marsupium wood. J. Ethenopharm., 35: 71-75.

Al-Awadi, F.M. and K.A. Gumaa, 1987. Studies on the activity of individual plants of an antidiabetic plant mixture. Acta Diabetol., 24: 37-41.
CrossRef  |  Direct Link  |  

Bell, G.I. and K.S. Polonsky, 2001. Diabetes mellitus and genetically programmed defects in β-cell function. Nature, 414: 788-791.
Direct Link  |  

Brownlee, M., 2001. Biochemistry and molecular cell biology of diabetic complications. Nature, 414: 813-820.
CrossRef  |  PubMed  |  Direct Link  |  

Cameron, O.G., Z. Kronfol, J.F. Grenden and B.J. Carroll, 1984. Hypothalamic-pituitary-adrenocortical activity in patients with diabetes mellitus. Arch. Gen. Psychiatry, 41: 1090-1095.
PubMed  |  

Chan, O., S. Chan, K. Iouye, M. Vrnic and S.G. Matthews, 2001. Molecular regulation of the Hypothalamus-Pituitary-Adrenal (HPA) axis in streptozotocin-induced diabetes: Effects of insulin treatment. Endocrinology, 142: 4872-4879.
Direct Link  |  

Coiro, V., R. Volpi, L. Capretti, G. Speroni and P. Caffarra et al., 1995. Low-dose corticotrophin-releasing hormone stimulation test in diabetes mellitus with or without neuropathy. Metabolism, 44: 538-542.
Direct Link  |  

Cryer, P.E. and J.E. Gerich, 1997. Hypoglycemia in Insulin-Dependent Diabetes Mellitus: Interplay of Insulin Excess and Comprised Glucose Counterregulation. In: Ellenberg and Rifkin's Diabetes Mellitus, Porte, Jr. D. and R.S. Sherwin (Eds.). Appleton and Lange, Stamford, CT, pp: 745-760.

De Nicola, A.F., O. Fridman, E.J. Del Castillo and V.G. Foglia, 1976. The influence of streptozotocin diabetes on adrenal function in male rats. Horm. Metabol. Res., 8: 388-392.

Doyle, P., F. Rohner-Jeanrenaud and B. Jeanrenaud, 1993. Local cerebral glucose utilization in brains of lean and genetically obese (fa/fa) rats. Am. J. Physiol., 264: E29-E36.
Direct Link  |  

El Tahir, K.E.H., M.M.S. Ashour and M.M. Al-Harbi, 1993. The Cardiovascular actions of the volatile oil of the black seed (Nigella sativa) in rats: Elucidation of the mechanisms of action. Gen. Pharmar., 24: 1123-1131.
CrossRef  |  

Fehm, H.L., R. Holl, E. Spath-Schwalbe, J. Born and K.H. Voigt, 1988. Ability of corticotropin releasing hormone to stimulate cortisol secretion independent from pituitary adrenocorticotropin. Life Sci., 42: 679-686.

Garcia Leme, J. and S.P. Farsky, 1993. Hormonal control of inflam- matory response. Mediators of Inflammation, 2: 181-198.
CrossRef  |  Direct Link  |  

Garcia Leme, J., L. Hamamura, R.H. Migliorini and M.P. Leite, 1973. Experimental diabetes and inflammatory reactions in the rat. Agents Actions, 3: 380-381.
CrossRef  |  Direct Link  |  

Horner, H., D. Packan and R. Sapolsky, 1990. Glucocorticoids inhibit glucose transport in cultured hippocampal neurons and glia. Neuroendocrinol., 52: 57-64.
Direct Link  |  

Hudson, J.I., M.S. Hudson, A.J. Rothschild, L. Vignati, A.F. Schatzberg and J.C. Melby, 1989. Abnormal results of dexamethasone suppression tests in non-depressed patients with diabetes mellitus. Arch. Gen. Psychiatry, 41: 1086-1089.

Ismail, M., Y. Ozbek and R. Ustun, 2003. Effects of Nigella sativa on serum concentration TSH and glucose in induced diabetic rabbits. J. Irish Vet., 56: 446-484.

Kadekaro, M., M. Ito and P.M.Gross, 1988. Local cerebral glucose utilization is increased in acutely adrenalectomized rats. Neuroendocrinology, 47: 329-334.

Khosia, P., D. Gupts and R.K. Nagpal, 1995. Effect of trigonella foenom graecum fenugreet on blood glucose in normal and diabetic rats. Ind. J. Physiol. Pharmacol., 39: 173-174.

Klip, A., A. Marette, D. Dimitrakouids, T. Ramlal and M. Shizq Varnic, 1992. Effect of diabetes on glucoregulation. From glucose transports to glucose metabolism in vivo. Diabetes Care, 15: 1747-1766.
Direct Link  |  

Lefebvre, P.J.and A.S. Luyckx, 1979. Glucagon and diabetes: A reappraisal. Diabetologia., 16: 347-354.
Direct Link  |  

Manisckam, M.M., M.A. Ramanthan, J.P. Jahromi, J. Chasouria and A.B. Ray, 1997. Antihyperglycemic activity of phenolics from Pterocapus marsupium. J. Nat. Products, 60: 609-610.
CrossRef  |  Direct Link  |  

McCall, A.L., 1992. The impact of diabetes on CNS. Diabetes, 41: 557-570.
Direct Link  |  

Palmieri, V., J.N. Bella, D.K. Arnett, J.E. Liu and A. Oberman et al., 2001. Effect of type 2 diabetes mellitus on left ventricular geometry and systolic function in hypertensive subjects: Hypertension genetic Epidemiology network Hyper GEN Study. Circulation, 103: 102-107.
CrossRef  |  PubMed  |  Direct Link  |  

Rai, V., U. Lyer and U.V. Mani, 1997. Effect of Tulasi Ocimum sanctum leaf powder supplementation on blood sugar levels serum lipids and tissue lipids in diabetic rats. Plant Foods Human Nut., 50: 9-16.
PubMed  |  Direct Link  |  

Rayfield, E.J., M.J. Ault, G.T. Keusch, M.J. Brothers, C. Nechemias and H. Smith, 1982. Infection and diabetes: The case for glucose control. Am. J. Med., 72: 439-450.
Direct Link  |  

Reagan, L.P., A.M. Magarinos and B.S. McAwen, 1999. Neurological changes induced by stress in streptozotocin diabetic rats. Ann. New York Acad. Sci., 893: 126-137.
Direct Link  |  

Reagan, L.P., A.M. Magarinos, L.R. Lucas, A. Van Bueren, A.L. McCall and B.S. McEwen, 1999. Regulation of GLUT-3 glucose transporter in the hippocampus of diabetic rats subjected to stress. Am. J. Physiol., 276: E879-E886.
Direct Link  |  

Roy, M., B. Collier and A. Roy, 1990. Hypothalamic-pituitary-adrenal axis dysregulation among diabetic outpatients. Psychiatry Res., 31: 31-37.
Direct Link  |  

Roy, M.S., A. Roy and W.T. Gallucci, 1993. The ovine corticotropin-releasing hormone-stimulation test in type I diabetic patients and controls: Suggestion of mild chronic hypercortisolism. Metabolism, 42: 696-700.
Direct Link  |  

Saks, D.B., 1997. Implication of the revised criteria for diagnosis and classification of diabetes mellitus. Clin. Chem., 43: 2230-2232.
Direct Link  |  

Sapolsky, R.M., 1996. Glucocorticoids and damage to the nervous system: The current state of confusion. Stress, 1: 1-19.
Direct Link  |  

Scribner, K.A., S.F Akana, C.D. Walker and M.F. Dolman, 1993. Streptozotocin-diabetic rats exhibit facilitated adrenocorticotropin responses to acute stress, but normal sensitivity to feedback by corticosteroids. Endocrinology, 133: 2667-2674.

Shapiro, E.T., K.S. Polonsky, G. Copinschi, D. Bosson, H. Tillil, J. Blackman, G. Lewis and E. Van Cauter, 1991. Nocturnal elevation of glucose levels during fasting in noninsulin-dependent diabetes. J. Clin. Endocrinol. Metabol., 72: 444-454.
Direct Link  |  

Taskinen, M.R., S. Lahdenpera and M. Syvanne, 1996. New insights into lipid metabolism in non-insulin-dependent diabetes mellitus. Ann. Med., 28: 335-340.
Direct Link  |  

Tich, R. and H. McDevit, 1996. Insulin dependent diabetes mellitus. Cell, 85: 291-297.

Tuk, J., 2003. Effect of Nigella sativa on liver necrosis. Vet. Anim. Sci., 27: 141-152.

Unger, R.H., 1978. Role of glucagon in the pathogenesis of diabetes: The status of the controversy. Metabolism., 27: 1691-1709.

Vlassara, H., M. Brownlee and A. Cerami, 1984. Accumulation of diabetic rat peripheral nerve myelin by macrophages increases with the presence of advanced glycosylation end products. J. Exp. Med., 160: 197-207.

Weitzman, E.D., D. Fukushima, C. Nogeire, H. Roffwarg, T.F. Gallagher and L. Hellman, 1971. Twenty-four-hour pattern of the episodic secretion of cortisol in normal subjects. J. Clin. Endocrinol. Metab., 33: 14-22.
Direct Link  |  

Yabe-Nishimura, C., 1998. Aldose reductase in glucose toxicity: A potential target for the prevention of diabetic complication. Pharmacol. Rev., 50: 21-34.
Direct Link  |  

Yenigum, M., 1997. Cardiovascular diabetes. Istanbul University printing house, Istanbul, Turkey, 43: 2230-2233.

Zaoui, A., Y. Cherrah, M.A. Lacaille-Dubois, A. Settaf, H. Amarouch and M. Hassar, 2000. Diuretic and hypotensive effects of Nigella sativa in the spontaneously hypertensive rat. Therapie, 55: 379-382, (In French).
PubMed  |  Direct Link  |  

Zimmet, P., K.G.M.M. Alberti and J. Shaw, 2001. Global and societal implications of the diabetes epidemic. Nature, 414: 782-787.
CrossRef  |  PubMed  |  Direct Link  |  

Zubaida, A., A. Basil, A. Abdullah and Bomosa, 2001. Effect of Nigella sativa (Black seed) and thymoquin on blood glucose in albino rats Ann. Saudi Med., 21: 18-27.

©  2019 Science Alert. All Rights Reserved
Fulltext PDF References Abstract