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International Journal of Pharmacology

Year: 2006 | Volume: 2 | Issue: 1 | Page No.: 104-109
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Research Article

Effects of Oral Administration of Water Extract of Nigella sativa on the Hypothalamus Pituitary Adrenal Axis in Experimental Diabetes

Kamal M.S. Mansi

ABSTRACT

The present study was designed to evaluate the role of water extract of Nigella sativa on the hypothalamus-pituitary-adrenal axis in alloxan-induced diabetic rats. Forty male white rats were divided into four experimental groups control, diabetic. N. sativa-treated and N. sativa-treated diabetic. At the end of the experimental period (3 weeks), animals in all three groups were fasted for 12 h and blood samples were taken for the determination of glucose levels, serum concentrations of insulin, glucagon, corticosterone and Adrenocorticotropic hormone (ACTH ) in four groups. It was found that water extract of Nigella sativa was investigated for hypoglycemic effect in diabetic rats and induced significant reduction in serum glucose from (19.83±1.25 Mmol L-1) in diabetic group to (9.7±1.10 Mmol L-1) in N. sativa- treated diabetic group. However the blood glucose still higher than the control and N. sativa- treated group, serum insulin increased from (0.54±0.22 Mu L-1) in control group to (0.65±0.06 Mu L-1) in N. sativa –treated group and still higher than control in N. sativa –treated diabetic (0.58±0,06 Mu L-1), serum corticosterone increased in diabetic group (580 ± 22.36 nmol L-1 )compared to control group (311±18.42 nmol L-1) and decreased in N. sativa-treated (238±16.53 nmol L-1) and in N. sativa treated diabetic group (378±19.65 nmol L-1) and still higher than control. Serum Adrenocorticotropic hormone (ACTH) increased in diabetic group (20.72±2.42 pmol L-1 ) compared to control group (13.82±1.83 pmol L-1) and still lower in N. sativa -treated (10.64±13 pmol L-1) and in N. sativa treated diabetic group (15.42±1.18 pmol L-1) compared to diabetic group. The results suggest the beneficial role of N. sativa as hypoglycemic agents and as a protective effect against pancreatic ß-cells damage from alloxan induced diabetes in rats by decreasing oxidative stress and preserving pancreatic ß -cells integrity and also suggest that the antidiabetic effect of N. sativa may be attributed to increased glucose metabolism by increasing the serum concentration of insulin and inhibited the hypothalamus-pituitary-adrenal axis.
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