• [email protected]
  • +971 507 888 742
Submit Manuscript
SciAlert
  • Home
  • Journals
  • Information
    • For Authors
    • For Referees
    • For Librarian
    • For Societies
  • Contact
  1. International Journal of Pharmacology
  2. Vol 2 (1), 2006
  3. 104-109
  • Online First
  • Current Issue
  • Previous Issues
  • More Information
    Aims and Scope Editorial Board Guide to Authors Article Processing Charges
    Submit a Manuscript

International Journal of Pharmacology

Year: 2006 | Volume: 2 | Issue: 1 | Page No.: 104-109
DOI: 10.3923/ijp.2006.104.109
crossmark

Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail
Research Article

Effects of Oral Administration of Water Extract of Nigella sativa on the Hypothalamus Pituitary Adrenal Axis in Experimental Diabetes

Kamal M.S. Mansi

ABSTRACT


The present study was designed to evaluate the role of water extract of Nigella sativa on the hypothalamus-pituitary-adrenal axis in alloxan-induced diabetic rats. Forty male white rats were divided into four experimental groups control, diabetic. N. sativa-treated and N. sativa-treated diabetic. At the end of the experimental period (3 weeks), animals in all three groups were fasted for 12 h and blood samples were taken for the determination of glucose levels, serum concentrations of insulin, glucagon, corticosterone and Adrenocorticotropic hormone (ACTH ) in four groups. It was found that water extract of Nigella sativa was investigated for hypoglycemic effect in diabetic rats and induced significant reduction in serum glucose from (19.83±1.25 Mmol L-1) in diabetic group to (9.7±1.10 Mmol L-1) in N. sativa- treated diabetic group. However the blood glucose still higher than the control and N. sativa- treated group, serum insulin increased from (0.54±0.22 Mu L-1) in control group to (0.65±0.06 Mu L-1) in N. sativa –treated group and still higher than control in N. sativa –treated diabetic (0.58±0,06 Mu L-1), serum corticosterone increased in diabetic group (580 ± 22.36 nmol L-1 )compared to control group (311±18.42 nmol L-1) and decreased in N. sativa-treated (238±16.53 nmol L-1) and in N. sativa treated diabetic group (378±19.65 nmol L-1) and still higher than control. Serum Adrenocorticotropic hormone (ACTH) increased in diabetic group (20.72±2.42 pmol L-1 ) compared to control group (13.82±1.83 pmol L-1) and still lower in N. sativa -treated (10.64±13 pmol L-1) and in N. sativa treated diabetic group (15.42±1.18 pmol L-1) compared to diabetic group. The results suggest the beneficial role of N. sativa as hypoglycemic agents and as a protective effect against pancreatic ß-cells damage from alloxan induced diabetes in rats by decreasing oxidative stress and preserving pancreatic ß -cells integrity and also suggest that the antidiabetic effect of N. sativa may be attributed to increased glucose metabolism by increasing the serum concentration of insulin and inhibited the hypothalamus-pituitary-adrenal axis.
PDF References Citation

How to cite this article

Kamal M.S. Mansi, 2006. Effects of Oral Administration of Water Extract of Nigella sativa on the Hypothalamus Pituitary Adrenal Axis in Experimental Diabetes. International Journal of Pharmacology, 2: 104-109.

DOI: 10.3923/ijp.2006.104.109

URL: https://scialert.net/abstract/?doi=ijp.2006.104.109

Search


REFERENCES


  1. Tich, R. and H. McDevit, 1996. Insulin dependent diabetes mellitus. Cell, 85: 291-297.

  2. Bell, G.I. and K.S. Polonsky, 2001. Diabetes mellitus and genetically programmed defects in β-cell function. Nature, 414: 788-791.
    Direct Link

  3. Zimmet, P., K.G.M.M. Alberti and J. Shaw, 2001. Global and societal implications of the diabetes epidemic. Nature, 414: 782-787.
    CrossRefPubMedDirect Link

  4. Klip, A., A. Marette, D. Dimitrakouids, T. Ramlal and M. Shizq Varnic, 1992. Effect of diabetes on glucoregulation. From glucose transports to glucose metabolism in vivo. Diabetes Care, 15: 1747-1766.
    Direct Link

  5. Taskinen, M.R., S. Lahdenpera and M. Syvanne, 1996. New insights into lipid metabolism in non-insulin-dependent diabetes mellitus. Ann. Med., 28: 335-340.
    Direct Link

  6. Vlassara, H., M. Brownlee and A. Cerami, 1984. Accumulation of diabetic rat peripheral nerve myelin by macrophages increases with the presence of advanced glycosylation end products. J. Exp. Med., 160: 197-207.

  7. Yabe-Nishimura, C., 1998. Aldose reductase in glucose toxicity: A potential target for the prevention of diabetic complication. Pharmacol. Rev., 50: 21-34.
    Direct Link

  8. Brownlee, M., 2001. Biochemistry and molecular cell biology of diabetic complications. Nature, 414: 813-820.
    CrossRefPubMedDirect Link

  9. Saks, D.B., 1997. Implication of the revised criteria for diagnosis and classification of diabetes mellitus. Clin. Chem., 43: 2230-2232.
    Direct Link

  10. Palmieri, V., J.N. Bella, D.K. Arnett, J.E. Liu and A. Oberman et al., 2001. Effect of type 2 diabetes mellitus on left ventricular geometry and systolic function in hypertensive subjects: Hypertension genetic Epidemiology network Hyper GEN Study. Circulation, 103: 102-107.
    CrossRefPubMedDirect Link

  11. Yenigum, M., 1997. Cardiovascular diabetes. Istanbul University printing house, Istanbul, Turkey, 43: 2230-2233.

  12. Garcia Leme, J., L. Hamamura, R.H. Migliorini and M.P. Leite, 1973. Experimental diabetes and inflammatory reactions in the rat. Agents Actions, 3: 380-381.
    CrossRefDirect Link

  13. Garcia Leme, J. and S.P. Farsky, 1993. Hormonal control of inflam- matory response. Mediators of Inflammation, 2: 181-198.
    CrossRefDirect Link

  14. Rayfield, E.J., M.J. Ault, G.T. Keusch, M.J. Brothers, C. Nechemias and H. Smith, 1982. Infection and diabetes: The case for glucose control. Am. J. Med., 72: 439-450.
    Direct Link

  15. Weitzman, E.D., D. Fukushima, C. Nogeire, H. Roffwarg, T.F. Gallagher and L. Hellman, 1971. Twenty-four-hour pattern of the episodic secretion of cortisol in normal subjects. J. Clin. Endocrinol. Metab., 33: 14-22.
    Direct Link

  16. Cameron, O.G., Z. Kronfol, J.F. Grenden and B.J. Carroll, 1984. Hypothalamic-pituitary-adrenocortical activity in patients with diabetes mellitus. Arch. Gen. Psychiatry, 41: 1090-1095.
    PubMed

  17. Coiro, V., R. Volpi, L. Capretti, G. Speroni and P. Caffarra et al., 1995. Low-dose corticotrophin-releasing hormone stimulation test in diabetes mellitus with or without neuropathy. Metabolism, 44: 538-542.
    Direct Link

  18. Roy, M., B. Collier and A. Roy, 1990. Hypothalamic-pituitary-adrenal axis dysregulation among diabetic outpatients. Psychiatry Res., 31: 31-37.
    Direct Link

  19. Roy, M.S., A. Roy and W.T. Gallucci, 1993. The ovine corticotropin-releasing hormone-stimulation test in type I diabetic patients and controls: Suggestion of mild chronic hypercortisolism. Metabolism, 42: 696-700.
    Direct Link

  20. Fehm, H.L., R. Holl, E. Spath-Schwalbe, J. Born and K.H. Voigt, 1988. Ability of corticotropin releasing hormone to stimulate cortisol secretion independent from pituitary adrenocorticotropin. Life Sci., 42: 679-686.

  21. Hudson, J.I., M.S. Hudson, A.J. Rothschild, L. Vignati, A.F. Schatzberg and J.C. Melby, 1989. Abnormal results of dexamethasone suppression tests in non-depressed patients with diabetes mellitus. Arch. Gen. Psychiatry, 41: 1086-1089.

  22. Sapolsky, R.M., 1996. Glucocorticoids and damage to the nervous system: The current state of confusion. Stress, 1: 1-19.
    Direct Link

  23. Kadekaro, M., M. Ito and P.M.Gross, 1988. Local cerebral glucose utilization is increased in acutely adrenalectomized rats. Neuroendocrinology, 47: 329-334.

  24. Doyle, P., F. Rohner-Jeanrenaud and B. Jeanrenaud, 1993. Local cerebral glucose utilization in brains of lean and genetically obese (fa/fa) rats. Am. J. Physiol., 264: E29-E36.
    Direct Link

  25. Horner, H., D. Packan and R. Sapolsky, 1990. Glucocorticoids inhibit glucose transport in cultured hippocampal neurons and glia. Neuroendocrinol., 52: 57-64.
    Direct Link

  26. Reagan, L.P., A.M. Magarinos and B.S. McAwen, 1999. Neurological changes induced by stress in streptozotocin diabetic rats. Ann. New York Acad. Sci., 893: 126-137.
    Direct Link

  27. McCall, A.L., 1992. The impact of diabetes on CNS. Diabetes, 41: 557-570.
    Direct Link

  28. Ahmad, F., P.M.M. Khalid, M. Khan, A.K. Chaubey, Rastogi and J.R. Kidwai, 1995. Hypoglycemic activity of pterocarpus marsupium wood. J. Ethenopharm., 35: 71-75.

  29. Khosia, P., D. Gupts and R.K. Nagpal, 1995. Effect of trigonella foenom graecum fenugreet on blood glucose in normal and diabetic rats. Ind. J. Physiol. Pharmacol., 39: 173-174.

  30. Rai, V., U. Lyer and U.V. Mani, 1997. Effect of Tulasi Ocimum sanctum leaf powder supplementation on blood sugar levels serum lipids and tissue lipids in diabetic rats. Plant Foods Human Nut., 50: 9-16.
    PubMedDirect Link

  31. Manisckam, M.M., M.A. Ramanthan, J.P. Jahromi, J. Chasouria and A.B. Ray, 1997. Antihyperglycemic activity of phenolics from Pterocapus marsupium. J. Nat. Products, 60: 609-610.
    CrossRefDirect Link

  32. Zubaida, A., A. Basil, A. Abdullah and Bomosa, 2001. Effect of Nigella sativa (Black seed) and thymoquin on blood glucose in albino rats Ann. Saudi Med., 21: 18-27.

  33. Agel, M. and R. Shaheen, 1996. Effects of the volatile oil of Nigella sativa seeds on the uterine smooth muscle of rat and guinea pig. J. Ethnopharmacol., 52: 23-26.
    CrossRefDirect Link

  34. El Tahir, K.E.H., M.M.S. Ashour and M.M. Al-Harbi, 1993. The Cardiovascular actions of the volatile oil of the black seed (Nigella sativa) in rats: Elucidation of the mechanisms of action. Gen. Pharmar., 24: 1123-1131.
    CrossRef

  35. Zaoui, A., Y. Cherrah, M.A. Lacaille-Dubois, A. Settaf, H. Amarouch and M. Hassar, 2000. Diuretic and hypotensive effects of Nigella sativa in the spontaneously hypertensive rat. Therapie, 55: 379-382, (In French).
    PubMedDirect Link

  36. Tuk, J., 2003. Effect of Nigella sativa on liver necrosis. Vet. Anim. Sci., 27: 141-152.

  37. Abdel, M.A., M. El Feki and E. Saleh, 1998. Effect of Nigella sativa, fish oil and localized on alloxan diabetic rats. Bioch. histopath studies. J. Egypt. Ger. Soc. Zool., 23: 237-265.

  38. Ismail, M., Y. Ozbek and R. Ustun, 2003. Effects of Nigella sativa on serum concentration TSH and glucose in induced diabetic rabbits. J. Irish Vet., 56: 446-484.

  39. Al-Awadi, F.M. and K.A. Gumaa, 1987. Studies on the activity of individual plants of an antidiabetic plant mixture. Acta Diabetol., 24: 37-41.
    CrossRefDirect Link

  40. Shapiro, E.T., K.S. Polonsky, G. Copinschi, D. Bosson, H. Tillil, J. Blackman, G. Lewis and E. Van Cauter, 1991. Nocturnal elevation of glucose levels during fasting in noninsulin-dependent diabetes. J. Clin. Endocrinol. Metabol., 72: 444-454.
    Direct Link

  41. Scribner, K.A., S.F Akana, C.D. Walker and M.F. Dolman, 1993. Streptozotocin-diabetic rats exhibit facilitated adrenocorticotropin responses to acute stress, but normal sensitivity to feedback by corticosteroids. Endocrinology, 133: 2667-2674.

  42. De Nicola, A.F., O. Fridman, E.J. Del Castillo and V.G. Foglia, 1976. The influence of streptozotocin diabetes on adrenal function in male rats. Horm. Metabol. Res., 8: 388-392.

  43. Chan, O., S. Chan, K. Iouye, M. Vrnic and S.G. Matthews, 2001. Molecular regulation of the Hypothalamus-Pituitary-Adrenal (HPA) axis in streptozotocin-induced diabetes: Effects of insulin treatment. Endocrinology, 142: 4872-4879.
    Direct Link

  44. Unger, R.H., 1978. Role of glucagon in the pathogenesis of diabetes: The status of the controversy. Metabolism., 27: 1691-1709.

  45. Lefebvre, P.J.and A.S. Luyckx, 1979. Glucagon and diabetes: A reappraisal. Diabetologia., 16: 347-354.
    Direct Link

  46. Reagan, L.P., A.M. Magarinos, L.R. Lucas, A. Van Bueren, A.L. McCall and B.S. McEwen, 1999. Regulation of GLUT-3 glucose transporter in the hippocampus of diabetic rats subjected to stress. Am. J. Physiol., 276: E879-E886.
    Direct Link

  47. Cryer, P.E. and J.E. Gerich, 1997. Hypoglycemia in Insulin-Dependent Diabetes Mellitus: Interplay of Insulin Excess and Comprised Glucose Counterregulation. In: Ellenberg and Rifkin's Diabetes Mellitus, Porte, Jr. D. and R.S. Sherwin (Eds.). Appleton and Lange, Stamford, CT, pp: 745-760.

Search


Related Articles

Leave a Comment


Your email address will not be published. Required fields are marked *

Useful Links

  • Journals
  • For Authors
  • For Referees
  • For Librarian
  • For Socities

Contact Us

Office Number 1128,
Tamani Arts Building,
Business Bay,
Deira, Dubai, UAE

Phone: +971 507 888 742
Email: [email protected]

About Science Alert

Science Alert is a technology platform and service provider for scholarly publishers, helping them to publish and distribute their content online. We provide a range of services, including hosting, design, and digital marketing, as well as analytics and other tools to help publishers understand their audience and optimize their content. Science Alert works with a wide variety of publishers, including academic societies, universities, and commercial publishers.

Follow Us
© Copyright Science Alert. All Rights Reserved