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Comparative Evaluation of Hypoglycaemic Activity of Two Medicinal Plants in Alloxan Diabetic Rats

Sathyanarayanan Srinivasan and Balasubramanian Karundevi
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Aqueous extracts of the seeds of fenugreek (Trigonella foenum graecum) and fruits of bitter gourd (Momordica charantia) were tested for their hypoglycemic activity. The aqueous extracts or just the solvent alone (control) were orally administered daily for 7 days (short term) or 15 days (long term) to alloxan-induced diabetic rats. Blood glucose and plasma insulin levels and the subsequent effect on Oral Glucose Tolerance Test (OGTT) were monitored at 3, 6 and 9 h after the last administration. Daily administration of T. foenum graecum for 7 days failed to induce any significant change in the blood glucose levels. However, an extended 15-day treatment regimen was found to significantly reduce the blood glucose levels. The blood glucose levels were found to decrease at 6 and 9 h after the final administration of the extract. In contrast, both 7 and 15 days daily administration of M. charantia reduced the blood glucose levels drastically (p<0.05 and p<0.01, respectively). Significant reduction in blood glucose levels was observed at 6 and 9 h after the short-term treatment and at 3, 6 and 9 h after the long-term treatment. Long term administration of both T. foenum graecum and M. charantia was found to decrease blood glucose levels during OGTT at 30, 60 and 90 min when compared to OGTT of diabetic animals. There was no change in plasma insulin levels subsequent to administration of either T. foenum graecum or M. charantia. The observed results indicate that T. foenum graecum and M. charantia have hypoglycemic effect. The fact that the plasma insulin levels were unaltered suggests that the probable mechanism does not involve β-cell and may be attributable to decrease in intestinal absorption of glucose.

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  How to cite this article:

Sathyanarayanan Srinivasan and Balasubramanian Karundevi , 2005. Comparative Evaluation of Hypoglycaemic Activity of Two Medicinal Plants in Alloxan Diabetic Rats. International Journal of Pharmacology, 1: 267-276.

DOI: 10.3923/ijp.2005.267.276



1:  Ivorra, M.D., M. Paya and A. Villar, 1989. A review of natural products and plants as potential antidiabetic drugs. J. Ethnopharmacol., 27: 243-275.
PubMed  |  Direct Link  |  

2:  Clark, C.M. and D.A. Lee, 1995. Prevention and treatment of the complications of diabetes mellitus. N. Engl. J. Med., 332: 1210-1217.

3:  Defronzo, R.A., R. Hendler and D. Simonson, 1982. Insulin resistance is a prominent feature of insulin-dependent diabetes. Diabetes, 31: 795-801.

4:  Jarvinen, Y.H. and V.A. Koivisto, 1984. Insulin sensitivity in newly-diagnosed type-I diabetics following ketoacidosis after a 3 month insulin therapy. J. Clin. Endocrinol. Metab., 59: 371-378.

5:  Jarvinen, Y.H. and V.A. Koivisto, 1986. Natural course of insulin resistance in type-I diabetes. N. Engl. J. Med., 315: 224-230.

6:  Khan, C.R and Y. Shechter, 1991. Insulin, Oral Hypoglycemic Agents and the Pharmacology of the Endocrine Pancreas. In: Goodman and Gillman`s The Pharmacological Basis of Therapeutics, Gilman, A.G., T.W. Rall, A.S. Nies and P. Taylor (Eds.). Pergamon Press, New York, pp: 1463-1495

7:  Atta-Ur-Rahman and K. Zaman, 1989. Medicinal plants with hypoglycemic activity. J. Ethnopharmacol., 26: 1-55.
CrossRef  |  PubMed  |  Direct Link  |  

8:  Platel, K. and K. Srinivasan, 1997. Plant foods in the management of diabetes mellitus: Vegetables as potential hypoglycaemic agents. Nahrung, 41: 68-74.
CrossRef  |  Direct Link  |  

9:  Grover, J.K., S. Yadav and V. Vats, 2002. Medicinal plants of India with anti-diabetic potential. J. Ethnopharmacol., 81: 81-100.
CrossRef  |  Direct Link  |  

10:  Shani, J., A. Goldschmied, B. Joseph, Z. Ahronson and F.G. Sulman, 1974. Hypoglycemic effect of Trigonella foenum graecum and Lupinus termis (Leguminosae) seeds and their major alkaloids in alloxan diabetic and normal rats. Arch. Intl. Pharmacodyn. Therapeut., 210: 27-37.

11:  Bever, B.O. and G.R. Zahnd, 1979. Plants with oral hypoglycemic action. Q. J. Crude Drug Res., 17: 139-196.

12:  Ribes, G., Y. Sauvaire, J.C. Baccou, G. Valette, D. Chenon, E.R. Trimble and M.M. Loubatieres-Mariani, 1984. Effects of fenugreek seeds on endocrine pancreatic secretions in dogs. Ann. Nutr. Metab., 28: 37-43.
PubMed  |  Direct Link  |  

13:  Valette, G., Y. Sauvaire, J.C. Baccou and G. Ribes, 1984. Hypocholesterolaemic effect of fenugreek seeds in dogs. Atherosclerosis, 50: 105-111.
CrossRef  |  PubMed  |  Direct Link  |  

14:  Ribes, G., Y. Sauvaire, C. da Costa, J.C. Baccou and M.M. Loubatieres-Mariani, 1986. Antidiabatic effects of subfractions from fenugreek in diabetic dogs. Proc. Soc. Exp. Biol. Med., 182: 156-166.

15:  Riyad, M.A., S.A.G. Abdul-Salam and S.S. Mohammad, 1988. Effect of fenugreek and lupine seeds on the development of experimental diabetes in rats. Planta Med., 54: 286-290.
CrossRef  |  Direct Link  |  

16:  Ravikumar, P. and C.V. Anuradha, 1999. Effect of fenugreek seeds o n blood lipid peroxidation and antioxidants in diabetic rats. Phytother. Res., 13: 197-201.

17:  Grover, J.K. and S.P. Yadav, 2004. Pharmacological actions and potential uses of Momordica charantia: A review. J. Ethnopharm., 93: 123-132.
CrossRef  |  PubMed  |  Direct Link  |  

18:  Leatherdale, B.A., R.K. Panesar, G. Singh, T.W. Abkins, C.J. Bailey and A.H.C. Bignell, 1981. Improvement in glucose tolerance due to Momordica charantia (karela). Br. Med. J., 282: 1823-1824.

19:  Bailey, C.J., C. Day, S.L. Turner and B.A. Leatherdale, 1985. Cerasee, a traditional treatment for diabetes. Studies in normal and streptozotocin diabetic mice. Diabetes Res., 2: 81-84.
PubMed  |  

20:  Welihinda, J., G. Arvidson, E. Gylfe, B. Hellman and E. Karlsson, 1982. The insulin-releasing activity of the tropical plant Momordica charantia. Acta. Biol. Med. Ger., 41: 1229-1240.
PubMed  |  Direct Link  |  

21:  Kedar, P. and C.H. Chakrabarti, 1982. Effects of bitter gourd (Momordica charantia) seed and glibenclamide in streptozotocin-induced diabetes mellitus. Indian J. Exp. Biol., 20: 232-235.

22:  Ahmed, I., M.S. Lakshni, M. Gillet, A. John and H. Raza, 2001. Hypotriglyceridemic and hypocholesterolemic effects of anti-diabetic Momordica charantia (karela) fruit extract in streptozotocin-induced diabetic rats. Diabetes Res. Clin. Pract., 51: 155-161.
CrossRef  |  PubMed  |  Direct Link  |  

23:  Saxena, A. and N.K. Vikram, 2004. Role of selected Indian plants in management of type 2 diabetes: A review. J. Altern. Complement Med., 10: 369-378.
CrossRef  |  Direct Link  |  

24:  Riley, V., 1960. . Adaptation of orbital sinus bleeding technique to rapid serial blood studies. Proc. Soc. Exp. Biol. Med., 104: 751-754.

25:  Trinder, P., 1969. Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Ann. Clin. Biochem., 6: 24-27.
CrossRef  |  Direct Link  |  

26:  Zar, H.H., 1990. Biostatistical Analysis. 1st Edn., Prentice Hall, USA

27:  Kiehm, T.G., J.W. Anderson and K. Ward, 1976. Beneficial effects of a high carbohydrate, high fiber diet in hyperglycemic men. Am. J. Clin. Nutr., 29: 895-899.

28:  Jenkins, D.J.A., D.V. Goff, A.R. Leeds, D.G.M.M. Alberti, T.M.S. Wolever, M.A. Gassull and T.D.R. Hockaday, 1976. Unabsorbable carbohydrates and diabetes: decreased post-prandial hyperglycemia. Lancet, 2: 172-174.

29:  Jenkins, D.J.A., T.M.S. Wolever, T.D.R. Hockaday, A.R. Leeds and H. Richard et al., 1977. Treatment of diabetes with guar gum. Lancet, 2: 779-780.

30:  Jenkins, D.J.A., A.R. Leeds, M.A. Gassull, B. Cochet and D.G.M.M. Alberti, 1977. Decrease in postprandial insulin and glucose tolerance: importance of viscosity. Br. Med. J., 1: 1392-1394.

31:  Jenkins, D.J.A., T.M.S. Wolever, A.R. Leeds, M.A. Gassull and P. Haisman et al., 1978. Dietary fibres, fiber analogues and glucose tolerance: importance of viscosity. Br. Med. J., 1: 1392-1394.

32:  Anderson, J.W. and K. Ward, 1978. Long term effects of high carbohydrate, high fiber diets on glucose and lipid metabolism: A preliminary report on p atients with diabetes. Diabetes Care, 1: 77-82.

33:  Anderson, J.W. and K. Ward, 1979. High-carbohydrate, high-fiber diets for insulin-treated men with diabetes mellitus. Am. J. Clin. Nutr., 32: 2312-2321.

34:  Anderson, J.W. and W.L. Chen, 1979. Plant fiber carbohydrate and lipid metabolism. Am. J. Clin. Nutr., 32: 346-363.

35:  Hozumi, T., M. Yoshida, Y. Ishida, H. Mimoto, J. Sawa, K. Doi and T. Kazumi, 1995. Long-term effects of dietary fiber supplementation on serum glucose and lipoprotein levels in diabetic rats fed a high cholesterol diet. Endocr. J., 42: 187-192.
Direct Link  |  

36:  Anderson, J.W., W.R. Midgley and B. Wedman, 1979. Fiber and diabetes. Diabetes Care., 2: 369-379.

37:  Karunanayake, E.H., J. Welihinda, S.R. Sirimanne and G.S. Adorai, 1984. Oral hypoglycaemic activity of some medicinal plants of Sri Lanka. J. Ethnopharmacol., 11: 223-231.
CrossRef  |  Direct Link  |  

38:  Karunanayake, E.H., S. Jeevathayaparan and K.H. Tennekoon, 1990. Effect of Momordica charantia fruit juice on streptozotocin-induced diabetes in rats. J. Ethnopharmacol., 30: 199-204.
PubMed  |  

39:  Day, C., T. Cartwright, J. Provost and C.J. Bailey, 1990. Hypoglycemic effect of Momordica charantia extracts. Planta Med., 56: 426-429.

40:  Sharma, R.D., T.C. Raghuram and N.S. Rao, 1990. Effect of fenugreek seeds on blood glucose and serum lipids in type I diabetes. Eur. J. Clin. Nutr., 44: 301-306.
PubMed  |  Direct Link  |  

41:  Higashino, H., A. Suzuki, Y. Tanaka and K. Pootakham, 1992. Hypoglycemic effects of Siamese Momordica charantia and Phyllanthus urinariaextracts in streptozotocin-induced diabetic rats (the 1st report). Nippon Yakurigaku Zasshi, 100: 415-421.

42:  Ali, L., A.K. Azad-khan, M.I. Rouf-Mamun, M. Mosihuzzaman, N. Nahar, M. Nur-e-Alam and B. Rokeya, 1993. Studies on hypoglycemic effect of fruit pulp, seed and whole plant of momordica charantia on normal and diabetic model rats. Plant Med., 59: 408-412.
Direct Link  |  

43:  Cakici, I., C. Hurmoglu, B. Tunctan, N. Abacioglu, I. Kanzik and B. Sener, 1994. Hypoglycaemic effect of Momordica charantia extracts in normoglycaemic orcyproheptadine-induced hyperglycaemic mice. J. Ethnopharmacol., 44: 117-121.

44:  Ali, L., A.K. Azad-Khan, Z. Hassan, M. Mosihuzzaman and N. Nahar et al., 1995. Characterization of the hypoglycemic effects of Trigonella foenum graecum seed. Planta Med., 61: 358-360.
PubMed  |  ISI  |  

45:  Khosla, P., D.D. Gupta and R.K. Nagpal, 1995. Effect of Trigonella foenum graecum (Fenugreek) on blood glucose in normal and diabetic rats. Indian. J. Physiol. Pharmacol., 39: 173-174.
PubMed  |  Direct Link  |  

46:  Sarkar, S., M. Pranava and R. Marita, 1996. Demonstration of the hypoglycemic action of Momordica charantia in a validated animal model of diabetes. Pharmacol. Res., 33: 1-4.
CrossRef  |  PubMed  |  

47:  Genet, S., R.K. Kale and N. Z. Baquer, 1999. Effects of vanadate, insulin and fenugreek (Trigonella foenum graecum) on creatine kinase levels in tissues of diabetic rat. Indian. J. Exp. Biol., 37: 200-202.

48:  Jayasooriya, A.P., M. Sakono, C. Yukizaki, M. Kawano, K. Yamamoto and N. Fukuda, 2000. Effects of Momordica charantia powder on serum glucose levels and various lipid parameters in rats fed with cholesterol-free and cholesterol-enriched diets. J. Ethnophamacol., 72: 331-336.
Direct Link  |  

49:  Raza, H., I. Ahmed, A. John and A.K. Sharma, 2000. Modulation of xenobiotic metabolism and oxidative stress in chronic streptozotocin-induced diabetic rats fed with Momordica charantia fruit extract. J. Biochem. Mol. Toxicol., 14: 131-139.
CrossRef  |  PubMed  |  Direct Link  |  

50:  Sitasawad, S.L., Y. Shewade and R. Bhonde, 2000. Role of bittergourd fruit juice in stz-induced diabetic state in vivo and in vitro. J. Ethnopharmacol., 73: 71-79.
PubMed  |  Direct Link  |  

51:  Anuradha, C.V. and P. Ravikumar, 2001. Restoration on tissue antioxidants by fenugreek seeds (Trigonella foenum graecum) in alloxan-diabetic rats. Indian. J. Physiol. Pharmacol., 45: 408-420.
PubMed  |  Direct Link  |  

52:  Grover, J.K., V. Vats, S.S. Rathi and R. Dawar, 2001. Traditional Indian anti-diabetic plants attenuate progression of renal damage in streptozotocin induced diabetic mice. J. Ethnopharmacol., 76: 233-238.
Direct Link  |  

53:  Vikrant, V., J.K. Grover, N. Tandon, S.S. Rathi and N. Gupta, 2001. Treatment with extracts of Momordica charantia and Eugenia jambolana prevents hyperglycemia and hyperinsulinemia in fructose fed rats. J. Ethnopharmacol., 76: 139-143.
CrossRef  |  Direct Link  |  

54:  Zia, T., S.N. Hasnain and S.K. Hasan, 2001. Evaluation of the oral hypoglycaemic effect of Trigonella foenum-graecum L. (methi) in normal mice. J. Ethnopharmacol., 75: 191-195.
Direct Link  |  

55:  Vats, V., J.K. Grover and S.S. Rathi, 2002. Evaluation of anti-hyperglycemic and hypoglycemic effect of Trigonella foenum-graecum Linn, Ocimum sanctum Linn and Pterocarpus marsupium Linn in normal and alloxanized diabetic rats. J. Ethnopharmacol., 79: 95-100.
PubMed  |  Direct Link  |  

56:  Simpson, H.C.R., R.W. Simpson, S. Lousley, R.D. Carter, M. Geekie, T.D.R. Hockaday and J.I. Mann, 1981. A high carbohydrate leguminous fiber diet improves all aspects of diabetic control. Lancet, 1: 1-5.

57:  Riccardi, G., A. Rivellese, D. Pacioni, S. Genovese, P. Mastranzo and M. Mancini, 1984. Separate influence of dietary carbohydrate and fiber on the metabolic control in diabetes. Diabetologia, 26: 116-121.

58:  Hannan, J.M.A., B. Rokeya, O. Faruque, N. Nahar, M. Mosihuzzaman, A.K.A. Khan and L. Ali, 2003. Effect of soluble dietary fibre fraction of Trigonella foenum graecum on glycemic, insulinemic, lipidemic and platelet aggregation status of type 2 diabetic model rats. J. Ethnopharmacol., 88: 73-77.
CrossRef  |  Direct Link  |  

59:  Petit, P., Y. Sauvaire, G. Ponsin, M. Manteghetti, A. Fave and G. Ribes, 1993. Effects of a fenugreek seed extract on feeding behaviour in the rat: Metabolic-endocrine correlates. Pharmacol. Biochem. Behav., 45: 369-374.
CrossRef  |  PubMed  |  Direct Link  |  

60:  Parillo, M., G. Riccardi, D. Pacioni, C. Iovine and F. Contaldo et al., 1988. Metabolic consequences of feeding a high-carbohydrate, high-fiber diet to diabetic patients with chronic kidney failure. Am. J. Clin. Nutr., 48: 255-259.
Direct Link  |  

61:  Holman, R.R., T.L. Dornan, V. Mayon-White, J. Howard-Williams and C. Orde-Peckar et al., 1998. Prevention of deterioration of renal and sensory-nerve function by more intensive management of insulin-dependent diabetic patients. Lancet, 1: 204-208.

62:  Nelson, R.W., 1989. The role of fiber in managing diabetes mellitus. Vet. Med., 84: 1156-1160.

63:  O`Connor, N., J. Tredger and L. Morgan, 1987. Viscosity differences between various guar gums. Diabetologia, 30: 612-615.

64:  Blackburn, N.A. and I.T. Johnson, 1981. The effect of guar gum on the viscosity of the gastrointestinal contents and on glucose uptake from the perfused jejunum in the rat. Br. J. Nutr., 46: 239-246.

65:  Lund, E.K., J.M. Gee, J.C. Brown, P.J. Wood and I.T. Johnson, 1989. Effect of oat gum on the physical properties of the gastrointestinal contents and on the uptake of D-galactose and cholesterol by rat small intestine in vitro. Br. J. Nutr., 62: 91-101.

66:  Caspary, W.F., B. Elsenhans, U. Sufke, M. Ptok, R. Blume, B. Lembcke and W. Crutzfeldt, 1980. Effect of dietary fiber on absorption and motility. Front. Horm. Res., 7: 202-217.

67:  Blackburn, N.A. and I.T. Johnson, 1983. The influence of guar gum on the movements of inulin, glucose and fluid in rat intestine during perfusion in vivo. Pflügers Arch., 397: 144-148.

68:  Blackburn, N.A., J.S. Redfern, H. Jarjis, A.M. Holgate and I. Hanning et al., 1984. The mechanism of action of guar gum in improving glucose tolerance in man. Clin. Sci., 66: 329-336.

69:  Flourie, B., N. Vidon, C.H. Florent and J.J. Bernier, 1984. Effect of pectin on jejunal glucose absorption and unstirred layer thickness in normal men. Gut, 25: 936-941.
Direct Link  |  

70:  Roberts, F.G., H.A. Smith, A.G. Low and P.R. Ellis, 1990. Influence of Wheat Breads Containing Guar Flour Supplements of High and Low Molecular Weights on Viscosity Ofjejunal Digesta in the Pig. In: Dietary Fibre: Chemical and Biolog-Ical Aspects, Southgate D.A.T., K. Waldren, I.T. Johnson and G.R. Fenwick (Eds.). Royal Society of Chemistry, Cambridge, UK., pp:164-168

71:  Jenkins, D.J.A., A.L. Jenkins, T.M.S. Wolver, G.R. Collier, A.V. Rao and L.U. Thompson, 1987. Starchy foods and fiber: Reduced rate of digestion and improved carbohydrate metabolism. Scand. J. Gastroenterol., 22: 132-141.

72:  Johansen, H.N. and K.E. Bach-Knudsen, 1994. Effects of wheat-flour and oat mill fractions on jejunal flow, starch degradation and absorption of glucose over an isolated loop of jejunum in pigs. Br. J. Nutr., 72: 299-313.

73:  Khanna, P., S.C. Jain, A. Panagariya and V.P. Dixit, 1981. Hypoglycemic activity of polypeptide-P from a plant source. J. Nat. Prod., 44: 648-655.
CrossRef  |  Direct Link  |  

74:  Ng, T.B., C.M. Wong, W.W. Li and H.W. Yeung, 1986. Insulin-like molecules in Momordica charantia seeds. J. Ethnopharmacol., 15: 107-117.
CrossRef  |  Direct Link  |  

75:  Meir, P. and Z. Yaniv, 1985. An in vitro study on the effect of Momordica charantia on glucose uptake and glucose metabolism in rats. Planta Med., 1: 12-16.

76:  Welihinda, J. and E.H. Karunanayake, 1986. Extra-pancreatic effects of Momordica charantia in rats. J. Ethnopharmacol., 17: 247-256.

77:  Shibib, B.A., L.A. Khan and R. Rahman, 1993. Hypoglycaemic activity of Coccinia indica and Momordica charantia in diabetic rats: Depression of the hepatic gluconeogenic enzymes glucose-6-phosphatase and fructose-1,6-bisphosphatase and elevation of both liver and red-cell shunt enzyme glucose-6-phosphate dehydrogenase. Biochem. J., 292: 267-270.
CrossRef  |  PubMed  |  Direct Link  |  

78:  Banerjee, S., A. Sengupta, J. Banerji, P. Adikary and A. Chatterjee, 1994. Studies on hypoglycemic effect of indigenous herbs. Indian J. Pharmacol., 26: 229-230.

79:  Anuradha, V. and P. Jamuna, 1989. Dietary fiber contents of selected vegetables and fruits. J. Food Sci. Tecnol., 26: 354-356.

80:  Sreedevi and A. Chaturvedi, 1993. Effect of vegetable fiber on post prandial glycemia. Plant Foods Hum. Nutr., 44: 71-78.
CrossRef  |  Direct Link  |  

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