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Research Article

Antibacterial Activity of Different Extracts of Sundakai (Solanum torvum) Fruit Coat

M. Sivapriya, R. Dinesha, R. Harsha, S.S.T. Gowda and L. Srinivas
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The main aim of this study was to find a new natural, non toxic, effective antibiotic from plant extract. In the present study, Solanum torvum (Sundakai) was selected. The antibacterial activity of different extracts of Solanum torvum (Sundakai) fruit coat was studied in vitro against human pathogenic strains. The Water Extract (WE) and Ethanol Water Extract (EWE) was found to be effective against all bacterial strains and inhibition is comparable to that of commercial antibiotics, choramphenicol and streptomycin. Initial screening of antibacterial activity was done by disc diffusion method in Nutrient agar medium. Minimum inhibition concentration was done serial dilution method. The MIC values of the Water Extract (WE), Ethanol Water Extract (EWE) and Ethanol Extract (EE) ranged from 9.6 to 19.2 μg mL-1. It was observed that there was a correlation between the amounts of polyphenols and flavonoids content and effective antibacterial activities of the inhibited extracts. These results indicate that Sundakai coat may be yet an another source of natural antibiotic. Further, this study reaffirms the ethanomedicinal property of S. torvum plant.

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M. Sivapriya, R. Dinesha, R. Harsha, S.S.T. Gowda and L. Srinivas, 2011. Antibacterial Activity of Different Extracts of Sundakai (Solanum torvum) Fruit Coat. International Journal of Biological Chemistry, 5: 61-67.

DOI: 10.3923/ijbc.2011.61.67

Received: March 31, 2010; Accepted: May 19, 2010; Published: August 30, 2010


In developing countries, Infectious diseases remain the main cause of high mortality rates recorded by WHO (1996). The treatment of infectious diseases is mainly based on the use of antibiotics. In recent years, a number of antibiotics have lost their effectiveness due to development of resistant strains (Shahidi Bonjar, 2004), mostly through the expression of resistance genes (Davis, 1994; Service, 1995). In addition to this problem, antibiotics are sometimes associated with adverse effects including hypersensitivity, immune-suppression and allergic reactions (Ahmad et al., 1998). Therefore, there is a need to develop alternative antimicrobial drugs for the treatment of infectious diseases from various sources such as medicinal plants. Indigenous herbal remedies are widely used against many infectious diseases, but only few of them have been studied chemically and biologically in order to identify their active constituents (Otshudi et al., 2000). Medicinal plants are also known to be used as food preservative due to its antimicrobial activity (Sunilson et al., 2009). Solanum torvum (Solanaceae) commonly known as Sundakai is a small shrub, of which its edible fruits are used as essential ingredients in Thai and Indian cuisine (Iida et al., 2005). It has been used ethnomedically as a tonic and haematopoetic agent and for treatment of pain (Ambasta, 1992; Daziel, 1937; Watt and Breyer-Brandwijk, 1962). The Antiviral, anti-ulcerogenic property of leaf extract has been reported (Arthan et al., 2002; Nguelefack et al., 2008). Leaf extract of S. torvum has been shown to process antibacterial activity (Valsaraj et al., 1997; Wiart et al., 2004). Sporadic alkaloids, saponins, sapogenins, flavonoids and glycosides have been reported from S. torvum (Arthan et al., 2002; Chung et al., 1998; Dopke et al., 1975; Lu et al., 2009). Nevertheless, there is no information about the antibacterial property of seed coat extract of S. torvum and its constituents.

Here we report the antibacterial property of Sundakai fruit coat extracts with various solvents or solvent mixture in order to ascertain the maximum antibiotic effect evoked by the fruit coat.


Chemicals: Quercetin, β-carotene, α-tocopherol were purchased from Sigma Chemical Co., USA. Agar, beef extract, yeast extract, peptone were purchased from Himedia Private L., India in Jan 2006. All other chemicals and reagents were of analytical grade and solvents were distilled before use.

Plant material: Sundakai (Solanum torvum) was obtained from authentic sources of Ramanagara, Karnataka, India in the month of August to October 2007. The identification of the plant was confirmed by G.R. Shivamurthy, Taxonomist, University of Mysore, Mysore, Karnataka, India. The herbarium of the plant was deposited in the ABCRI against voucher no-ABCRI 7/2007.

Preparation of sundakai fruit coat extracts: The fruit coat extracts were prepared by suspending 10 g of coat powder into 100 mL of distilled water, ethanol, ethanol : water (1:1), hexane and acetone separately. The resultant solution was vortexed thoroughly for 15 min and kept overnight at 4°C. The resultant suspension was frozen and thawed, centrifuged for 20 min at 4°C and 10,000 rpm. The water extract was lyophilized at -37°C and referred to as WE (Water Extract of Sundakai coat). The Ethanol: Water (1: 1) extract was evaporated at 37°C till the alcohol gets evaporated and the remaining water was lyophilized at-37°C and referred to as EWE (Ethanol Water Extract (1:1) of Sundakai coat). Similarly, ethanol extract, hexane extract and acetone extract were concentrated separately by evaporating at 37°C to a brown residue are referred as EE (Ethanol Extract of Sundakai coat), HE (Hexane Extract of Sundakai coat) and AE (Acetone Extract of Sundakai coat).

Ten milligram of each concentrated dried extract was dissolved in 1.0 mL of corresponding solvent or solvent mixtures and mixed. The solution was filtered in 0.45 micron microbial filter and stored at -20°C for further studies. The standard antibiotics were used at the concentrations based on the normal dose given for the infection.

Antimicrobial activity
Microbial strains:
Authentic pure clinical isolated cultures of human pathogenic bacteria, Escherichia coli, Vibrio cholerae, Staphylococcus aureus, Streptococcus, Bacillus subtilis, Klebsiella pneumoniae, Salmonella cibrum, Salmonella typhimurium, Pseudomonas sp., Proteus vulgarigus were obtained from Department of Microbiology, Adichunchanagiri Institute of Medical Sciences (AIMS), B.G. Nagara, Karnataka, India.

Agar well diffusion method: Antibacterial activity of various extracts of Sundakai coat was evaluated by the well diffusion method on nutrient agar medium (Forbes et al., 1990). This was confirmed by the inhibitory effect on bacterial growth as reflected by the inhibited zone compared to known antibiotics. The sterile nutrient agar medium (20 mL) in petridishes was uniformly smeared using sterile cotton swabs with test pure cultures of Escherichia coli, Vibrio cholerae, Streptococcus, Staphylococcus aureus, Bacillus subtilis, Klebsiella, Salmonella typhimurium, Salmonella cibrum, Proteus vulgarigus and Pseudomonas. The nutrient agar media was prepared by dissolving 0.3 beef extract, 0.3 yeast extract, 0.5 peptone, 0.5 NaCl and 1.5% agar in 1liter of distilled water. The wells of 5 mm diameter were made using sterile cork borer in each petriplates and the various extracts of Sundakai fruit coat were added, a blank well loaded without test compound was regarded as control. For each treatment 10 replicates were maintained. The plates were incubated at 37°C for 24 h and the resulting zone of inhibition was measured by comparing control and the standard antibiotic.

Determination of Minimum Inhibitory Concentration (MIC): The minimum inhibitory concentration of different extracts of Sundakai fruit coat were determined by serial dilution in the nutrient agar, with concentrations ranging from 5, 10, 20, 25, 50, 75 and 100 μg mL-1. The inoculum was prepared from fresh overnight broth culture in nutrient broth. Plates were incubated for 24 h at 37°C. The MIC was recorded as lowest extract concentration demonstrating no visible growth in the broth (Karou et al., 2005).

Proximate analysis: The proximate composition of different solvents extracts of Sundakai fruit coat were done such as, total protein (Bradford, 1976), total sugars (Dubois et al., 1956), total polyphenols (Kujala et al., 2000), total flavinoids (Woisky and Salatino, 1998), ascorbic acid (Sadasivam and Manickam, 1996) and α-tocopherol (Kivack and Mert, 2001) were determined according to the standard methods.

Statistical analysis: Statistical analysis was done in SPSS (Windows Version 10.0.1 Software Inc., New York) using a one-sided student’s t-test. All results refer to Mean±SD. p<0.05 was considered as statistically significant as comparing to relevant controls.


The fruits of Sundakai (Solanum torvum) have been reported to be widely used in traditional medicine as tonics for diarrhea, stomachache and for treating cough and pain. In the present study, we have observed that Sundakai fruit coat exhibit significant antibacterial activity against pure strains of pathogenic bacteria.

The results summarized in Table 1, indicate that among all the tested extracts, only the Water Extract (WE) and Ethanol Water Extract (EWE) exhibited effective antibacterial activity against E. coli, V. cholerae, S. aurenus, B. subtilis, S. cibrum, S. tryphimurium and Pseudomonas sp. Inhibition zone diameters between 20 and 26 mm). Water extract and Ethanol Water extract were as potent as standard antibiotics, chloramphenicol and streptomycin (Table 1). In general all the pure strains of microorganism were not susceptible to the hexane and acetone extract of Sundakai fruit coat.

Water extract ethanol water and ethanol extracts when tested in agar dilution assays for determining Minimum Inhibitory Concentration (MIC), it was observed that they all posses antibacterial growth activities with MIC values ranging from 9.6 to 19.2 μg mL-1. The tested extracts showed comparable MIC values with standard antibiotics which ranged from 11.7-20 μg mL-1 (Table 2). Thus the extracts were as potent as standard antibiotics in inhibiting the growth of bacterial strains.

Table 1: Antibacterial activity of various solvent or solvent mixture extracts of Sundakai fruit coat in comparison with commercial antibiotics
Image for - Antibacterial Activity of Different Extracts of Sundakai (Solanum torvum) Fruit Coat
The results are Mean±SD (n = 10). WE: Water extract, EWE: Ethanol water (1:1) extract, EE: Ethanol extract, HE: Hexane extract, AE: Acetone extract, G:Gentamycin, Cp: Chloramphenicol, Sm: Streptomycin

Table 2: Minimum inhibitory concentration (MIC) of solvent or solvent mixture extracts of Sundakai fruit coat in serial dilution method
Image for - Antibacterial Activity of Different Extracts of Sundakai (Solanum torvum) Fruit Coat
The results are Mean±SD (n = 10). WE: Water extract, EWE: Ethanol water (1:1) extract, EE: Ethanol extract, HE: Hexane extract, AE: Acetone extract, G:Gentamycin, Cp: Chloramphenicol, Sm: Streptomycin

Table 3: Proximate analysis of various solvent or solvent mixture extracts of Sundakai coat
Image for - Antibacterial Activity of Different Extracts of Sundakai (Solanum torvum) Fruit Coat
The results are Mean±SD (n = 10). Concentration expressed as mg/g. WE: Water extract, EWE: Ethanol water (1:1) extract, EE: Ethanol extract, HE: Hexane extract, AE: Acetone extract

In order to ascertain the components responsible for antibacterial activity, proximate analysis of various solvent extracts of Sundakai fruit coat was carried out. In Table 3, it appeared that Water Extract (WE) and Ethanol Water Extract (EWE) (1:1) of coat had the highest content of polyphenols and flavonoids, in comparison to that of acetone and hexane extract. Considerable amount of α-tocopherol was present in all the extracts where as protein, sugars and ascorbic acid was in negligible amounts. It was interesting to observe that there is a correlation between the high amount of polyphenols and flavonoids content and effective antibacterial activities of Water extract and Ethanol water extract. Therefore it can be concluded that the antibacterial activity may be due to the presence of high concentration of polyphenols and flavonoids of the inhibiting extract.

Polyphenols and flavonoids are reported to be important antimicrobial components (Chung et al., 1998; Karou et al., 2005). There are also enough documented date, which suggests that there is a positive correlation between total phenolic content and antimicrobial activity (Shan et al., 2007; Wu et al., 2006; Kudo et al., 2004). Flavonoids isolated from the leaves of Pluchea carolinensis has shown antibacterial activity (Cordova et al., 2006). The flavonoids like kaempferol, kaempferol-3-O-galactoside, rutin (quercetin-3-Orutinosid), genistin (genistein-7-O-glucoside) and orientin (luteolin-8-C-glucoside) were isolated from methanolic extract of Linum capitatum Flower are also known to have antibacterial activity (Ilic et al., 2004). The antibacterial activity of flavonoids and polyphenolic compounds might be due to their ability to complex with bacterial cell wall and therefore, inhibiting the microbial growth.

In conclusion, we have shown that extracts of Sundakai fruit coat exhibit significant antibacterial activity against pathogenic bacteria when compared to standard antibiotics. The inhibited extracts showed high polyphenols and flavonoids content. Therefore this Sundakai coat may be yet another source of natural antibiotic. This study rein firms the ethanomedicinal property of S. torvum.


The authors acknowledge the Adichunchanagiri Mahasamstana Mutt and Shikshana Trust for providing facilities in the Adichunchanagiri Biotechnology and Cancer Research Institute (ABCRI) for carrying out this study. We acknowledge microbiology department, Adichunchanagiri Institute of Medical Sciences (AIMS), B.G. Nagara, Karnataka, India for providing bacterial strains.


1:  Ahmad, I., Z. Mehmood and F. Mohammad, 1998. Screening of some Indian medicinal plants for their antimicrobial properties. J. Ethnopharmacol., 62: 183-193.
CrossRef  |  PubMed  |  Direct Link  |  

2:  Ambasta, S.P., 1992. The Useful Plants of India. CSIR., New Delhi, India

3:  Sunilson, J.A.J., R. Suraj, G. Rejitha, K. Anandarajagopal, A.V.A.G. Kumari and P. Promwichit, 2009. In vitro antimicrobial evaluation of Zingiber officinale, Curcuma longa and Alpinia galanga extracts as natural food preservatives. Am. J. Food Technol., 4: 192-200.
CrossRef  |  Direct Link  |  

4:  Arthan, D., J. Svasti, P. Kittakoop, D. Pittayakhachonwut, M. Tanticharoen and Y. Thebtaranonth, 2002. Antiviral iosflavonoid sulfate and steroidal glycosides from the fruits of Solanum torvum. Phytochemistrym, 59: 459-463.
CrossRef  |  

5:  Bonjar, G.H.S., 2004. New approaches in screening for antibacterials in plants. Asian J. Plant Sci., 3: 55-60.
CrossRef  |  Direct Link  |  

6:  Bradford, M.M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72: 248-254.
CrossRef  |  PubMed  |  Direct Link  |  

7:  Chung, K.T., T.Y. Wong, C.I. Wei, Y.W. Huang and Y. Lin, 1998. Tannins and human health: A review. Crit. Rev. Food Sci. Nutr., 38: 421-464.
CrossRef  |  PubMed  |  Direct Link  |  

8:  Davies, J., 1994. Inactivation of antibiotics and the dissemination of resistance genes. Science, 264: 375-382.
CrossRef  |  Direct Link  |  

9:  Daziel, J.M., 1937. The Useful Plants Tropical Africa. Crown Agents, London

10:  Dopke, W., C. Nogueiras and U. Hess, 1975. The steroid-alkaloid and sapogenin content of Solanum torvum. Pharmazie, 30: 755-755.
PubMed  |  

11:  DuBois, M., K.A. Gilles, J.K. Hamilton, P.A. Rebers and F. Smith, 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem., 28: 350-356.
CrossRef  |  Direct Link  |  

12:  Forbes, B.A., D.F. Sahm, A.S. Weissfeld and E.A. Trevino, 1990. Methods for Testing Antimicrobial Effectiveness. In: Bailey Scott's Diagnostic Microbiology, Baron, E.J., L.R. Peterson and S.M. Finegold (Eds.). Mosby Co., St Louis, Missouri, MI., pp: 171-194

13:  Iida, Y., Y. Yanai, M. Ono, T. Ikeda and T. Nohara, 2005. Three unusual 22-_-O-23-Hydroxy-(5_)-spirostanol Glycosides from the Fruits of Solanum torvum. Chem. Pharma. Bull., 53: 1122-1125.
Direct Link  |  

14:  Karou, D., H.M. Dicko, J. Simpore and S.A. Traore, 2005. Antioxidant and antibacterial activities of polyphenols from ethanomedicinal plants from Burkina Faso. Afr. J. Biotechnol., 4: 823-828.
Direct Link  |  

15:  Kivack, B. and T. Mert, 2001. Quantitative determination of α-tocopherol in Arbutus unedo by TLC-densitometry and colorimetry. Fitoterapia, 72: 656-661.
CrossRef  |  Direct Link  |  

16:  Hara-Kudo, Y., A. Kobayashi, Y. Sugita-Konishi and K. Kondo, 2004. Antibacterial activity of plants used in cooking for aroma and taste. J. Food Prot., 67: 2820-2824.
PubMed  |  

17:  Kujala, T.S., J.M. Loponen, K.D. Klika and K. Pihlaja, 2000. Phenolics and betacyanins in red beetroot (Beta vulgaris) root: Distribution and effect of cold storage on the content of total phenolics and three individual compounds. J. Agric. Food Chem., 48: 5338-5342.
CrossRef  |  Direct Link  |  

18:  Lu, Y., J. Luo, X. Huang and L. Kong, 2009. Four new steroidal glycosides from Solanum torvum and their cytotoxic activities. Steroids, 74: 95-101.
CrossRef  |  PubMed  |  

19:  Nguelefack, T.B., C.B. Feumebo, G. Ateufack, P. Watcho and S. Tatsimo et al., 2008. Anti-ulcerogenic properties of the aqueous and methanol extracts from the leaves of Solanum torvum Swartz (Solanaceae) in rats. J. Ethnopharmacol., 119: 135-140.
CrossRef  |  PubMed  |  Direct Link  |  

20:  Otshudi, A.L., A. Vercruysse and A. Foriers, 2000. Contribution to the ethnobotanical, phytochemical and pharmacological studies of traditionally used medicinal plants in the treatment of dysentery and diarrhoea in Lomela area, Democratic Republic of Congo (DRC). J. Ethnopharmacol., 71: 411-423.
CrossRef  |  Direct Link  |  

21:  Sadasivam, S. and A. Manickam, 1996. Biochemical Methods. 2nd Edn., New Age International (P) Ltd. Publishers, New Delhi, India

22:  Service, R.F., 1995. Antibiotics that resist resistance. Science, 270: 724-727.
CrossRef  |  PubMed  |  

23:  Shan, B., Y.Z. Cai, J.D. Brooks and H. Corke, 2007. The in vitro antibacterial activity of dietary spice and medicinal herb extracts. Int. J. Food Microbiol., 117: 112-119.
CrossRef  |  Direct Link  |  

24:  Ilic, S.B., S.S. Konstantinovic and Z.B. Todorovic, 2004. Antimicrobial activity of bioactive component from flower of Linum capitatum kit. Physics Chem. Technol., 3: 73-77.
Direct Link  |  

25:  Valsaraj, R., P. Pushpangadan, U.W. Smitt, A. Adsersen and U. Nyman, 1997. Antimicrobial screening of selected medicinal plants from India. J. Ethnopharmacol., 58: 75-83.
CrossRef  |  Direct Link  |  

26:  Watt, J.M. and M.G. Breyer-Brandwijk, 1962. The Medicinal and Poisonous Plants of Southern and Eastern Africa. 2nd Edn., E and S Liningstone Ltd., London, UK., Pages: 1457

27:  WHO., 1996. Resistance to antimicrobial agents. Bull. World Health Org., 71: 335-336.

28:  Wiart, C., S. Mogana, S. Khalifah, M. Mahan and S. Ismail et al., 2004. Antimicrobial screening of plants usd for traditional medicine in the state of perak, Peninsular Malaysia. Fitoterapia, 75: 68-73.
CrossRef  |  

29:  Cordova, W.H.P., L.G. Mesa, A.L.P. Hill, C.N. Lima, G.D. Lamas, M.O. Suarez and R.S. Dominguez, 2006. Antimicrobial activity of crude extracts and flavinoids from leaves of Pluchea carolinensis (jacq.) g. don. Pharmacologyonline, 3: 757-761.
Direct Link  |  

30:  Woisky, R.G. and A. Salatino, 1998. Analysis of propolis: Some parameters and procedures for chemical quality control. J. Apicult. Res., 37: 99-105.
Direct Link  |  

31:  Wu, C., F. Chen, X. Wang, H.J. Kim, G.Q. He, V. Haley-Zitlin and G. Huang, 2006. Antioxidant constituents in feverfew (Tanacetum parthenium) extract and their chromatographic quantification. Food Chem., 96: 220-227.
CrossRef  |  

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