Leaf anatomy of Iridaceae shows variations. Rudall (1994) gave a general description of leaf anatomy in Iridaceae and the importance of leaf anatomical characters for the systematic of the family is assessed by means of a cladistic analysis utilizing characters from morphology, anatomy and biochemistry. Although there is a high homoplasty among both morphological and anatomical characters, leaf anatomy provides some significant data for Iridaceae systematic, especially at the tribal level. There has not been any anatomical study on leaves of I. schachtii which is an endemic species.
MATERIALS AND METHODS
I. schachtii spreads between 1200-1700 m on open stony hills around Salt Lake, Central Anatolia, Turkey (Davis, 1984). Plant specimens were collected from Konya, Kulu on May 2004. Specimens were prepared as a herbarium material Fig. 1 and plant as a whole preserved in 70% ethanol solution.
Cross-sections and surface sections were taken by hand from individuals having violet and yellow flowers. Staining were made with safranin. Cross-sections were preserved in 10% glycerin. Mean epidermal and stoma cells numbers in mm2 of surface section were determined. Cell counts were made by using Leica DM-LS2 research microscope and photographs were taken with DFC-320 digital camera.
||Herbarium specimen of I. schachtii (violet and yellow
RESULTS AND DISCUSSION
Small and thin leaves are annual. Leaves are linear-lanceolate and show parallel
|| Epidermal and stoma cells
|| Papillae (arrow) and stoma are in cross section
|| Prismatic crystal (arrow)
Leaves are unifacial ensiform type. End walls of epidermal cells are straight
Small papillae are present on epidermis. Stomas are amaryllis type. Stoma appeared
embedded in the epidermis (Fig. 3). Prismatic crystals, running
longitudinal to the axis, were observed (Fig. 4).
|| Cross section of a leaf
|| Parenchyma cells with thick cell wall (arrows)
|| Parenchyma cells that have tannen like structures
According to Wu and Cutler (1985), diversity of shape and size of styloid
crystals are taxonomically important characteristics in Iris species.
It was determined that there is no difference in epidermal and stoma cells
between individuals with different flower color.
|| Sclerenchyma bundles at the leaf apex
|| Vascular bundle sheath
Mean number of epidermal and stoma cells in mm2 was found respectively
as; 193.95 and 99.05.
Photosynthetic region at mesophyll cells is continuous along the leaf axis. Mesophyll cells are getting larger awards the center and encircle the intercellular spaces (Fig. 5). Cell walls of parenchyma cells are relatively thick (Fig. 6). When stained, tannen like structures were observed in parenchyma cells (Fig. 7).
Bundles of sclerenchyma cells are at leaves margins and vascular bundles (Fig. 8). Vascular bundles are lined in rows. Xylem of vascular bundles is directed towards the leaf center. Vascular bundle sheath consists of outer layer of thin walled parenchyma cells and there is also a discontinuous inner layer of 2-3 sclerenchyma cells (Fig. 9).
The prismatic crystals observed in I. schachtii will be investigated in other organs of the plant and evaluated whether they have a taxonomical importance or not. This endemic plant with two different flower colors is a valuable ornamental plant and can provide economical income.