Subscribe Now Subscribe Today
Research Article

Effectiveness of RAPD Marker in Generating Polymorphism in Different Chicken Population

M.B.R. Mollah , M.S. Alam , F.B. Islam and M.A. Ali
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

A study was conducted to know the effectiveness of Randomly Amplified Polymorphic DNA (RAPD) marker in generating polymorphism in different chicken populations. Four of the twenty random primer screened yielded distinct RAPD profiles. Among 39 fragments amplified from these 4 primers, 25 of them showed polymorphism. The average number of amplified bands per primer ranged from 9 to 11. The study revealed that RAPD markers were effective in detecting polymorphism in different chicken breeds. However, comparatively large numbers of random primers were required to detect satisfactory polymorphism in different chicken populations.

Related Articles in ASCI
Similar Articles in this Journal
Search in Google Scholar
View Citation
Report Citation

  How to cite this article:

M.B.R. Mollah , M.S. Alam , F.B. Islam and M.A. Ali , 2005. Effectiveness of RAPD Marker in Generating Polymorphism in Different Chicken Population. Biotechnology, 4: 73-75.

DOI: 10.3923/biotech.2005.73.75


1:  Dodgson, J.B., H.H. Cheng and R. Okimoto, 1997. DNA marker technology: A revolution in animal genetics. Poult. Sci., 76: 1108-1114.
PubMed  |  

2:  Crooijmans, R.P., A.F. Groen, A.J. van Kampen, S. van der Beek, J.J. van der Poel and M.A. Groenen, 1996. Microsatellite polymorphism in commercial broiler and layer lines estimated using pooled blood samples. Poult. Sci., 75: 904-909.
PubMed  |  

3:  Takahasi, H., K. Nirasawa, M. Nagamine, M. Tsudzuki and Y. Yamamoto, 1998. Genetic relationships among Japanese native breeds of chicken based on microsatellite DNA polymorphism. J. Heredity, 89: 543-546.

4:  Singh, R.V. and D. Sharma, 2002. Within- and between-strain genetic variability in White Leghorn population detected through RAPD markers. Br. Poult. Sci., 43: 33-37.
CrossRef  |  PubMed  |  Direct Link  |  

5:  Welsh, J. and M. McClelland, 1990. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res., 18: 7213-7218.
CrossRef  |  PubMed  |  Direct Link  |  

6:  Smith, E.J., C.P. Jones, J. Bartlett and K.E. Nestor, 1996. Use of Randomly Amplified Polymorphic DNA markers for the genetic analysis of relatedness and diversity in chickens and turkeys. Poult. Sci., 75: 579-584.
PubMed  |  Direct Link  |  

7:  Zhang, X., G.R. McDaniel and J.J. Giambrone, 1995. Random amplified polymorphic DNA comparisons among broiler lines selected for incidence of tibial dyschondroplasia. Poult. Sci., 74: 1253-1258.

8:  Zhang, X., F.C. Leung, D.K.O. Chen, Y. Chen and C. Wu, 2002. Comparative analysis of allozyme, random amplified polymorphic DNA and micro satellite polymorphism on Chinese native chickens. Poult. Sci., 81: 1093-1098.
PubMed  |  Direct Link  |  

9:  Alam, M.S., A. Popplewell and N. Maclean, 1996. Germ line transmission and expression of alacZ containing transgene in tilapia (Oreochromis niloticus). Transgenic Res., 5: 87-95.
CrossRef  |  Direct Link  |  

10:  Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski and S.V. Tingey, 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res., 18: 6531-6535.
CrossRef  |  PubMed  |  Direct Link  |  

11:  Sharma, D. and R.V. Singh, 2001. Randomly amplified polymorphic DNA polymorphism in Rhodes Island Red chicken. Ind. J. Poult. Sci., 36: 1-4.

12:  Sharma, D., K.B.C.A. Rao, H.P. Singh and S.M. Totey, 1998. Randomly Amplified Polymorphic DNA (RAPD) for evaluating genetic relationships among varieties of guinea fowl. Genet. Anal.: Biomol. Eng., 14: 125-128.
CrossRef  |  PubMed  |  Direct Link  |  

13:  Shivaraman, G.K., D. Sharma, H.W. Haunshi and H.W. Sharma, 2001. Randomly amplified polymorphic DNA polymorphism among native and exotic chicken breeds. Ind. J. Poult. Sci., 36: 141-146.
Direct Link  |  

14:  Wei, R., M.R. Dentine and J.J. Bitgood, 1997. Random amplified polymorphic DNA markers in crosses between inbred lines of Rhode Island Red and White Leghorn chickens. Anim. Genet., 28: 291-294.

15:  Hoelzel, A.R., 1992. Molecular Genetic Analysis of Populations A Practical Approach. Oxford University Press, New York, pp: 50-58.

©  2021 Science Alert. All Rights Reserved