Aghighi S
Bahonar University University of Kerman, Iran
G.H. Shahidi Bonjar
Bahonar University University of Kerman, Iran
I. Saadoun
Jordan University of Science and Technology, Jordan
ABSTRACT
This is the first report on antifungal activity of a new strain of Streptomyces plicatus (strain101) against four Iranian phytopathogenic isolates of Verticillium dahliae. In the recent decades, biological control of plant diseases has received more attention mainly as a response to public concern about the use of hazardous chemicals in the environment. Soil Actinomycetes particularly Streptomyces spp. enhance soil fertility and have antagonistic activity against wide range of soil-borne plant pathogens. In search for metabolites of soil Actinomycetes having antifungal activity against four isolates of the cosmopolitan pathogen, Verticillium dahliae Klebahn, 110 isolates were screened. Among all, strain101 that was identified as a new strain of S. plicatus, showed high level of activity in Agar disk and Well-diffusion methods. S. plicatus was propagated in submerged cultures and active crude was prepared upon which some biological characterizations performed. The active metabolite (s) is polar, soluble in H2O and methanol but insoluble in chloroform, dichloromethane or hexane. Antifungal activity composed of two types, mycelial inhibition, inhibition of microsclerotia and melanin production. Antifungal gene from S. plicatus Strain 101 may be a useful candidate for genetic engineering of agriculturally important crop plants for increased tolerance against V. dahliae.
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How to cite this article
Aghighi S, G.H. Shahidi Bonjar and I. Saadoun, 2004. First Report of Antifungal Properties of a New Strain of Streptomyces plicatus
(Strain101) Against Four Iranian Phytopathogenic Isolates of Verticillium
dahliae, A New Horizon in Biocontrol Agents. Biotechnology, 3: 90-97.
DOI: 10.3923/biotech.2004.90.97
URL: https://scialert.net/abstract/?doi=biotech.2004.90.97
DOI: 10.3923/biotech.2004.90.97
URL: https://scialert.net/abstract/?doi=biotech.2004.90.97
REFERENCES
- Rowe, R.C. and D.L. Powelson, 2002. Potato early dying: Management challenges in a changing production environment. Plant Dis., 86: 1184-1193.
CrossRefDirect Link - Lopez-Escudero, F.J. and M.A. Blanco-Lopez, 2000. Control of Verticillium Wilt by Soil Solarization in Established Olive Orchards in Andalucia (Southern Spain). In: Advances in Verticillium Research and Disease Management, Tjamos, E.C., R.C. Rowe, J.B. Heale and D.R. Fravel (Eds.). The APS Press, St. Paul, Minnesota, USA., pp: 332-335.
- Tjamos, E.C., D.A. Biris and E.J. Paplomatas, 1991. Recovery of olive trees with verticillium wilt after individual application of soil solarization in established olive orchards. Plant Dis., 75: 557-562.
Direct Link - Berg, G., C. Knaape, G. Ballin and D. Seidel, 1994. Biological control of Verticillium dahliae kleb. by natural occurring rhizosphere bacteria. Arch. Phytopathol. Pflanz., 29: 249-262.
CrossRef - Lee, J.Y. and B.K. Hwang, 2002. Diversity of antifungal actinomycetes in various vegetative soils of Korea. Can. J. Microbiol., 48: 407-417.
Direct Link - Kong, L.D., D.D. Tzeng and C.H. Yang, 2001. Generation of PCR-based DNA fragments for specific detection of Streptomyces saraceticus N45. Proc. Natl. Sci. Council Roc., 25: 119-127.
Direct Link - Valois, D., K. Fayad, T. Barasubiye, M. Garon, C. Dery, R. Brzezinski and C. Beaulieu, 1996. Glucanolytic actinomycetes antagonistic to Phytophthora fragariae var. rubi, the causal agent of raspberry root rot. Applied Environ Microbiol., 62: 1630-1635.
Direct Link - Merriman, P.R., R.D. Price, J.F. Kollmorgen, T. Piggott and E.H. Ridge, 1974. Effect of seed inoculation with Bacillus subtilis and Streptomyces griseus on the growth of cereals and carrots. Aust. J. Agric. Res., 25: 219-226.
CrossRefDirect Link - Brown, M.E., 1974. Seed and root bacterization. Annu. Rev. Phytopathol., 12: 181-197.
CrossRefDirect Link - Saadoun, I., F. Al-Momani, H. Malkawi and M.J. Mohammad, 1999. Isolation, identification and analysis of antibacterial activity of soil streptomycetes isolates from North Jordan. Microbios, 100: 41-46.
Direct Link - Saadoun, I. and R. Gharaibeh, 2002. The Streptomycetes flora of Jordan and its potential as a source of antibiotics active against antibiotic resistant Gram negative bacteria. World J. Microbiol. Biotechnol., 18: 465-470.
Direct Link - El-Tarabily, K.A., M.H. Soliman, A.H. Nassar, H.A. Al-Hassani, K. Sivasithamparam, F. McKenna and G.E. Hardy, 2000. Biological control of Sclerotinia minor using a chitinolytic bacterium and actinomycetes. Plant Pathol., 49: 573-583.
Direct Link - Shirling, E.B. and D. Gottlieb, 1966. Methods for characterization of Streptomyces species. Int. J. Syst. Evol. Microbiol., 16: 313-340.
CrossRefDirect Link - Prauser, H., 1964. Aptness and application of colour codes for exact description of colours of Streptomyces. Z. Allg. Mikrobiol., 4: 95-98.
PubMed - Bonjar, G.H.S., 2004. New approaches in screening for antibacterials in plants. Asian J. Plant Sci., 3: 55-60.
CrossRefDirect Link - Moreno, A.B., A.M. Pozo, M. Borja and B.S. Segundo, 2003. Activity of antifungal protein from Aspergillus giganteus against Botrytis cinerea. Phytopathology, 93: 1344-1352.
Direct Link - Brogue, K., I. Chet, M. Holliday, R. Cressman and P. Biddle et al., 1991. Transgenic plants with enhanced resistance to the fungal pathogen Rhizoctonia solani. Science, 254: 1194-1197.
CrossRefDirect Link - Selitrennikoff, C.P., 2001. Antifungal proteins. Applied Environ. Microbiol., 67: 2883-2894.
CrossRefPubMedDirect Link