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Research Article

In vitro Rapid Regeneration of Plantlets from Leaf Explant of Watermelon (Citrullus lanatus Thumb.)

R.S. Sultana , M.A. Bari , M.H. Rahman , M.M. Rahman , N.A. Siddique and N. Khatun

Plantlets regeneration were achieved in callus culture of leaf segments from five days old field grown seedlings of Citrulus lanatus Thumb. Callus induction and plant regeneration at various frequencies were observed using different concentrations and combinations of growth regulators. The highest percentage of callus induction was observed in MS medium supplemented with 2.5 mg L-1 2, 4-D. After transfer of this callus on MS medium supplemented with 1.0 mg L-1 BA+0.2 mg L-1 NAA induce adventitious shoots and developed into plant by further subculture in the same medium. NAA (0.1 mg L-1) was found effective in the production of root. Plantlets were acclimatized and subsequently transferred to the field. Survival of the plantlets under ex vitro condition was 80%.

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  How to cite this article:

R.S. Sultana , M.A. Bari , M.H. Rahman , M.M. Rahman , N.A. Siddique and N. Khatun , 2004. In vitro Rapid Regeneration of Plantlets from Leaf Explant of Watermelon (Citrullus lanatus Thumb.). Biotechnology, 3: 131-135.

DOI: 10.3923/biotech.2004.131.135


Ahad, A.R., M.H. Islam, K. kuzzaman and O.I. Joarder, 1994. Plant regeneration from immature and mature axis of watermelons. Plant Tissue Cult., 2: 39-44.

Begum, S., L. Hakim and M.A. Azam, 1995. Efficient regeneration of plants from leaf base derived callus in sugarcane. Plant Tissue Cult., 5: 1-5.

Bhattacharya, S. and S. Dasgupta, 2001. In vitro Regeneration of Bauhinia variegata Linn. Proceedings of the 4th International Conference Plant Tissue Culture, Nov. 1-3, Dhaka, pp:16-16.

Chee, P.P., 1990. High frequency of somatic embryogenesis and recovery of fertile cucumber plants. Hortic. Sci., 25: 792-793.

Dong, J.Z. and S.R. Jia, 1991. High efficiency plant regeneration from cotyledons of watermelon (Citrullus vulgaris). Plant Cell Rep., 9: 559-562.

Gambley, R.L. and W.A. Dodd, 1990. An in vitro technique for production of de novo of multiple shoots in cotyledon explants of cucumber (Cucumis sativus L.). Plant Cell Tissue Organ Cult., 20: 177-183.
Direct Link  |  

Gliozeris, S., A. Tamosiunas and L. Stuopyte, 2001. Effects of BAP and 2, 4-D on in vitro regeneration of some cultivars of Caladium hortulanum. Proceedings of the 4th International Conference Plant Tissue Culture, Nov. 1-3, Dhaka, pp: 26-26.

Halder, T. and V.N. Gadgil, 1982. Morphogenesis in some plant species of the family cucurbitaceae. Proceedings of the Symposia on Tissue Culture of Economically Important Plants, (STCEIP'82), Singapore, pp: 98-103.

Hoque, A., R. Islam and O.I. Joarder, 1995. In vitro plantlets differentiation in kakrol (Momordica dioica Roxb.). Plant Tissue Cult., 5: 119-124.

Islam, R., P.K. Sarkar, A.T.M. Naderuzzaman and O.I. Joarder, 1994. In vitro regeneration of plants from cotyledons of Momordica charantia L. Plant Tissue Cult., 4: 105-109.

Jelaska, S., 1974. Embryogenesis and organogenesis in pumpkin explants. Physiol. Plant., 31: 257-261.
CrossRef  |  

Jha, S. and T.B. Jha, 1989. Micropropagation of Caphaelis ipecacuanha. Plant Cell Rep., 8: 437-439.

Karim, M.Z., M.N. Amin, M.A. Hossain, S. Islam, F. Hossin and R. Alam, 2002. Micropropagation of two sugarcane (Saccharum officinarum) varieties from callus culture. J. Biol. Sci., 2: 682-685.
CrossRef  |  Direct Link  |  

Mathukumar, B., D.I. Arokiasasamy and S. John Britto, 2000. In vitro propagation of Datura metel L. from hypocotyls explants. Plant Tissue Cult., 10: 39-44.

Murashige, T. and F. Skoog, 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Planta., 15: 473-497.
CrossRef  |  Direct Link  |  

Nabi, S.A., M.M. Rashid, M. Al-Amin and M.G. Rasul, 2002. Organogenesis in teasle gourd (Momordica dioica Roxb.). Plant Tissue Cult., 12: 173-180.
Direct Link  |  

Rahman, S.M., M. Hossain, O.I. Joarder and R. Islam, 1991. Plant regeneration from cotyledons of an interspecific hybrid of cucurbita. Plant Tissue Cult., 1: 91-95.

Rahman, S.M., M. Hossain, R. Islam and O.I. Joarder, 1993. Plant regeneration from internode segments of Cucurbitaceae maxima Duch. Cucurbitaceae moschata Duch. Curr. Sci., 65: 562-564.

Vincent, K.A., M. Hariharan and K.M. Mathew, 1992. Embryogenesis and plantlet formation in tissue culture of Kaempferia galangal L. Phytomorphology, 42: 253-256.
Direct Link  |  

Wakhlu, A.K. and K.S. Barna, 1989. Callus initiation, growth and plant regeneration in Plantago ovata Forsk. cv. Gl-2. Plant Cell, Tissue Cell Organ Cult., 17: 235-241.

Wehner, T.C. and R.D. Locy, 1981. In vitro adventitious shoot and root formation of cultivars and lines of Cucumis sativa L. HortScience, 16: 759-760.

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