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Research Article
 

Genetic Impact of Dwarfing Genes (Rht1 and Rht2) for Improving Grain Yield in Wheat



Mahboob Ali Sial, M. Afzal Arain , M. Aslam Javed and K. D. Jamali
 
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ABSTRACT

Dwarfing genes (Rht1 and Rht2) have been extensively used for enhancing the grain yield in bread wheat. Ten semi-dwarf wheat genotypes having either Rht1 or Rht2 dwarfing genes were evaluated at Nuclear Institute of Agriculture, Tandojam, for yield and yield components. Significant differences in grain yield and yield components were observed in genotypes under study showing effects of dwarfing genes. Genotype SI88123 (Rht1) had medium plant height (95.25 cm), higher number of tillers/plant (6.90), and the highest grain yield/plant (8.10 g). Sarsabz (Rht1) with higher plant height (101.85 cm), reduced number of tillers per plant (3.55) and fertile spikes/plant had higher grain yield per spike (2.56 g). The short statured genotype SI8878 possessed significantly the highest number of grains/spike and considerably good yield/plant. Number of productive spikes established very high correlation with grain yield per plant in all the genotypes. The intensity of relationship varied from genotype to genotype.

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Mahboob Ali Sial, M. Afzal Arain , M. Aslam Javed and K. D. Jamali , 2002. Genetic Impact of Dwarfing Genes (Rht1 and Rht2) for Improving Grain Yield in Wheat. Asian Journal of Plant Sciences, 1: 254-256.

DOI: 10.3923/ajps.2002.254.256

URL: https://scialert.net/abstract/?doi=ajps.2002.254.256

Introduction

The semi-dwarfing or reduced height genes Rht1 (Rht-B1b) and Rht2 (Rht-D1b) have been extensively exploited for developing high yielding varieties with reduced plant height and lodging resistance (Gale and Youssefian, 1985; Rebetzke and Richards, 2000). These genes reduced plant height by decreasing the sensitivity of reproductive and somatic tissues to endogenous gibberellin. The reduced cell elongation results in a decreased internode length, shorter plant height, shorter coleoptile length and smaller seedling leaf area (Keyes et al., 1989; Hoogendoorn et al., 1990; Beharev et al., 1998; Worland et al., 1998). The ‘DARUMA’ wheat grown in Japan around 1900 is considered to be a semi-dwarf type having short genes (Rht1 and Rht2 ) served as the framework for the "Green Revolution" in early sixties. The Norin 10 was then introduced in 1946 and used to develop Norin 10/Brevor 14 by Vogel et al. (1956) and was subsequently used by Borlaug (1968) and many others as a primary source of Rht1 and Rht2 genes.

Earlier wheat breeders believed that the taller cultivars have higher yield potential as compared to short statured ones as they possess higher biomass and have a larger source to contribute to the final sink of grain yield (Briggle and Vogel, 1968). However, with the induction of semi-dwarfing genes into tall wheat varieties, the theories have been changed. The semi-dwarfing genes Rht1 and Rht2 have been known to modify the plant ideotype by changing its cell size and number, root weight, coleoptile length, leaf size, harvest index, grain yield, yield components, protein content, disease reaction and gibberellic acid (GA) insensitivity (Gale and Youssefian, 1985; Loskutova, 1998). There are twenty major Rht dwarfing wheat genes. Of these, 16 are GA-sensitive, in contrast to Rht1 and Rht2 (Konzak, 1987).

This study is aimed to see the genetic impact of dwarfing genes (Rht1 and Rht2 ) for improving grain yield in wheat genotypes developed at Nuclear Institute of Agriculture (NIA), Tandojam.

Materials and Methods

Ten semi-dwarf spring wheat (Triticum aestivum L.) genotypes, namely WRS01, SI8878, SI8887, SI88123, SI88126, SI88155, SI88171, SI88231, Soghat 90 and Sarsabz were seeded at experimental farm, Nuclear Institute of Agriculture, Tandojam in the fall of 1993-94. A Randomized Complete Block Design (RCBD) was used and each plot consisted six rows 5.0 m in length and 30 cm distance between rows. Twenty plants at random, from 4 central rows were selected for agronomic studies. Traits measured on each plot were:

(I) plant height (cm); (ii) number of tillers per plant; (iii) number of productive spikes per plant; (iv) number of spikelets per spike; (v) number of grains per spike; (vi) number of grains per spikelet, calculated by dividing the number of seeds/spike by the number of spikelets/spike; (vii) grain yield per spike (g) and (viii) grain yield per plant (g). The parentage/pedigree of the genotypes is given in Table 1.

Table 1: Parentage/ pedigree and possible carrier of semi-dwarf genes
Image for - Genetic Impact of Dwarfing Genes (Rht1 and Rht2) for Improving Grain Yield in Wheat
Source: Singh et al. (1989)

Data were statistically analyzed and means were compared using Duncan’s Multiple Range Test (Gomez and Gomez, 1983). Phenotypic correlation coefficients were worked out between grain yield and other traits in each genotype following Snedecor, (1956).

Results and Discussion

The analysis of variance showed significant (P≤ 0.01) mean squares for genotypes for all the characters, suggesting genetical differences among genotypes. The genotype SI8878 was the shortest (80.15cm) among all in plant height and had significantly the highest number of spikelets and grains/spike, which resulted in ultimate higher grain yield/plant (Table 2). This may not be true in every case as Pinthus and Levy (1983) reported that the presence of Rht1 and Rht2 genes caused no increase in grains/spike and the tall genotypes had the highest mean number of grains/spike. However, short statured wheat varieties are considered as a breeding prerequisite in many of the wheat growing countries of the world (Schillinger et al., 1998). Genotypes SI88123 and SI88126 both having Rht1 gene and plant height around 95 cm produced significantly more and fertile tillers per plant leading to higher grain yield/plant (8.10 and 6.78g, respectively) than other entries. These results suggested that increasing plant height up to certain extent may produce higher grain yield and support the idea of "tall dwarf" plant height model suggested by Gale and Law (1977). Busch and Rauch (1993) in their study on agronomic performance of tall versus short semi-dwarf lines did not found sufficient evidence to support the application of the "tall-dwarf" model in their hard red spring wheat germplasm. Culm length is controlled by polygenes as well as by the major Rht genes. Positive genotypic relationships between culm length and grain yield within the different Rht genes have been often reported (Pinthus, 1987). Interaction effects of the Rht genes and polygenes on culm length and grain yield of spring wheat were also illustrated by Beharev et al. (1988).

Table 2: Mean differences in eight agronomic traits of different wheat genotypes.
Image for - Genetic Impact of Dwarfing Genes (Rht1 and Rht2) for Improving Grain Yield in Wheat
Means followed by same letters do not differ significantly at 5 percent level.

Table 3: Correlation coefficient (r ) of seven different agronomic traits with grain yield/plant (g) of wheat genotypes.
Image for - Genetic Impact of Dwarfing Genes (Rht1 and Rht2) for Improving Grain Yield in Wheat
*,**,*** denote correlation coefficient values significant at 5, 1 and 0.1% levels, respectively.

They found no beneficial effect on grain yield attributable to the Rht alleles per se which indicate the possible advantage of selecting for tall polygenes within the semi-dwarf group. Sarsabz had the maximum plant height (101.85 cm) and minimum number of spikes, but significantly higher main spike yield (2.56 g) than other entries. Because Sarsabz headed earlier and had long grain filling duration, there was more conversion of source into sink, hence produced more heavier seeds as compared to other genotypes. SI8887 with comparable plant height to Sarsabz had significantly the highest number of spikelets per spike. Two genotypes WRS01 and SI88171 were inferior to all for most of the traits. The local check variety Soghat 90 (Rht2) with medium plant height (94.4 cm), gave the highest main spike yield (2.33 g) followed by Sarsabz (2.56 g). Sarsabz, SI8887 and SI88155 having statistically similar plant height were significantly taller than the remaining genotypes. These results agreed with Gale and Youssefian, (1985), Richards (1992), Beharev et al. (1998).

Genotype SI88123 had the highest plant yield which was mainly contributed by the higher number of spikes/plant. An increased number of spikes per unit area often results in high grain yield (Bingham, 1972; Villareal et al., 1992). Grain yield of wheat has been continuously increasing by genetic improvements and increased agronomic inputs. Selection for reduced plant height has resulted in lodging resistance cum better utilization of chemical fertilizers. Semi-dwarf spring cultivars having lodging resistance also do not require vernalization (Busch and Rauch, 1993).

Phenotypic correlations between plant yield (y) and other yield components (x) were computed in each genotype. In general, all the genotypes had similar trend, however, the intensity of this relationship varied (Table 3). The significant positive correlations between plant height and yield were observed in five genotypes viz.; SI88123, SI88231, SI88171, Soghat 90 and Sarsabz. Number of tillers/plant and number of productive spike/plant showed very strong correlations with grain yield in all the genotypes. Three genotypes SI8887, SI88171 and Soghat 90 showed significantly positive correlations for number of grains per spike and grain yield. Main spike yield exhibited positive correlation with plant yield; however, it was significant only in genotypes, viz. WRS01, SI8887, SI88155, SI88171 and Soghat 90. The yield is a polygenic phenomenon and depends on its associated components. Spikes per unit area is one of the important yield components and is considered as the pleiotropic effect of Norin 10 genes (Gale and Youssefian, 1984; Kertesz et al., 1991). Highly significant positive correlation between plant height and grain yield per plant in genotype SI88123, established higher grain yield than the remaining genotypes. All the measured traits in SI88171 and Soghat 90 had positive correlations with yield per plant showing visible effects of semi-dwarf genes on yield. The Norin 10 dwarfing genes, Rht1 (Rht-B1b) and Rht2 (Rht-D1b), reduced plant height and increase grain yield by altering the proportion of dry matter allocated to the grain (harvest index), and by reducing plant lodging, especially with increasing environmental yield potential (Waddington et al., 1986; Slafer et al., 1994; Rebetzke and Richards, 1999).

The ‘Norin 10’ based semi-dwarf wheats have been extensively used in the development of high yielding cultivars at global level. The results showed that number of tillers per plant possessed very strong positive correlation with grain yield per plant, while the extent of correlation between plant height and grain yield varied among the genotypes. The maximum grain yield was produced by the genotypes SI88123 and Soghat 90, which also exhibited significantly positive correlation between plant height and grain yield. The presence of dwarfing genes prevent excessive plant height, while minor gene variation for height could be exploited to select for taller, high yielding semi-dwarfs. Plant height being more heritable than yield, selection for taller plants in a given semi-dwarf population may serve as a useful selection criteria in early generations where huge segregating population is involved.

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