Effect of Seed Fortification with Pulse Sprout Extract on Crop Growth and Seed Yield in Rice Seeds
Rice is the staple food for over half of the Indian population, main emphasis
has to be laid on ways and means to fortify seeds organically for better seed
vigour. Seed fortification is one of the important seed invigouration treatment.
Germinated grains are good sources of ascorbic acid, riboflavin, choline, thiamine,
tocopherols and pantothenic acid which increases the nutritional quality. Extract
has prepared from the sprouted pulses. With these background, an experiment
was conducted during 2008 to study the effect of seed fortification with pulse
sprout extract on crop growth and seed yield in rice seeds. Field experiment
was conducted with the treatments include fortification of rice seeds with 2
and 3% of horse gram sprout extract and 4 and 5% of cowpea sprout extract and
dried back to original moisture content and untreated seeds served as control.
The observations made on crop growth and yield factors such as field emergence
(%), plant height (cm) at vegetative and maturity stages, dry matter production
(g plant-1), No. of tillers plant-1, No. of productive
tillers plant-1, chlorophyll content, panicle length (cm), No. of
seeds panicle-1, 1000 seed weight (g) and seed yield. Among these
treatments 2% horse gram sprout extract recorded the highest yield of 3951 kg
ha-1. The yield enhancement may be due to the presence of bioactive
substances in sprouted horse gram and cowpea extracts were found effective towards
yield maximization in rice seeds.
Received: April 04, 2013;
Accepted: May 13, 2013;
Published: September 09, 2013
Rice is the major staple food for more than two billion people in Asia and
one third of the calorific intake of nearly one billion people of Africa and
Latin America. The uninterrupted and disproportionate use of chemical fertilizers
over a longer period of time has resulted in deterioration of soil health and
reduced yield. To maintain long-term food production there is a need for sustainable
agricultural practices. This is one of the aims of organic farming and consumers
are prepared to pay higher prices for certified organic products. In many developing
countries agriculture is still largely based on low inputs, because farmers
cannot afford the high costs of chemical fertilizers and pesticides. For such
farmers, organic farming can provide a better economic alternative because the
advantages are two fold (1) the inputs are of lesser cost and (2) the produces
fetch higher price. Sprouting has often been proposed as a useful and easy process
for improving nutritional quality of legume seeds. Hence, it was hypothesized
that application of the nutrient extract from the sprouted pulses in the form
of seed fortification will enable better crop growth and productivity of rice.
Horse gram and cowpea are the low cost and easily available pulses in Tamil
Nadu. Seed fortification treatment mainly supplies nutrient to the seed to germinate
into vigorous seedlings. With these ideas, an attempt was made to explore the
effect of seed fortification with pulse sprout extract on crop growth, seed
yield and quality in rice seeds.
MATERIALS AND METHODS
Materials: The study was conducted in rice variety CO 43. Seeds of rice obtained from Paddy Breeding Station, Tamil Nadu Agricultural University, Coimbatore was used for the study. The seeds were cleaned before conducting the studies. Field trial was conducted at Wet lands, Tamil Nadu Agricultural University, Coimbatore during 2008. Horse gram and cowpea pulse sprout extract used for seed fortification.
Methods: Horse gram and cowpea seeds were soaked overnight and incubated in a wet cloth for 12 h to enable sprouting. A 100 g of sprouts were ground in a mixer-grinder by using ice cubes from 100 mL of water to prepare extracts of 100% concentration. The rice seeds var Co 43 were soaked in varied concentrations of both horse gram and cowpea sprout extracts for 12 h. Later, the seeds were dried back to original moisture content under shade. Untreated seeds were maintained as control. Field trial was conducted during January, `08 with three replications and a plot size of 2x8 m. The fortified rice var Co43 seeds were directed seed under wetland conditions. The field emergence was recorded and required thinning was done to maintain the spacing of 20x15 cm. The following observations were made on crop growth and yield factors such as field emergence (%), plant height (cm) at vegetative and maturity stages, dry matter production (g plant-1), No. of tillers plant-1, No. of productive tillers plant-1, chlorophyll content, Panicle length (cm), No. of seeds panicle-1, 1000 seed weight (g) and seed yield.
Statistical analysis: The data obtained from experiments were analyzed
by the F test for significance following the method Factorial Randomized
Block Design as described by Panse and Sukhatme (1985).
Wherever, necessary, the percent values were transformed to angular (Arc-sine)
values before analysis. The Critical Differences (CD) were calculated at 5%
probability level. The data were tested for statistical significance.
According to Table 1 among the fortification treatments evaluated,
control recorded the lowest field emergence (61.0%). Among the treatments, horse
gram 2% recorded the highest emergence (80.7) which was followed by 3% (78.7)
and it was on par with cowpea 4% (78.7%). No significant difference was observed
for the field emergence in the interaction between the treatmentxconcentration.
Significant differences were found in plant height due to seed fortification
treatments. Among the different treatments, the plant height was maximum recorded
in horse gram 2% in both vegetative (83.1 cm) and maturity stages (101.1 cm),
it was followed by cowpea 4% (82.0 cm) and horse gram 3% (80.2 cm) at vegetative
stage. At maturity stage, the maximum of 100 cm was recorded by horse gram 3%
and cowpea 4%. The minimum plant height was recorded by control and at both
vegetative (77.6 cm) and maturity stages (91.7 cm), respectively. The statistical
analysis revealed that significant differences were found in dry matter production
due to seed fortification treatments. Control recorded the minimum dry matter
production of 9.05 g at vegetative and 27.1 g at maturity stages, respectively.
Among the different treatments, the maximum dry matter production was recorded
by horse gram 2% extract in both vegetative (12.8 g) and maturity stages (38.4
g) followed by horse gram 3% extracts 10.6 g at vegetative stage.
|| Effect of seed fortification with pulse sprouts extract on
growth attributes of rice variety Co 43
|Figures in Parenthesis are arcsine values
|| Effect of seed fortification with pulse sprout extracts on
productive attributes of rice variety Co 43
In maturity stage, it was followed by 4% cowpea extract as 32.6 g. The datas
of accoding to Table 2 No. of tillers per plant significantly
differed due to fortification treatments. Control recorded the minimum number
of tillers per plant at both vegetative (16.2) as well as maturity stages (17.5).
Among the different treatments, the maximum number of tillers registered by
horse gram 2% extract at both vegetative (19.2) and maturity stages (20.9)and
it was followed by cowpea extract 4% at vegetative (18.6) and at maturity (20.8)
stages. Significant difference was observed in number of productive tillers
dueto fortification treatments. Control recorded the minimum number of productive
tillers plant at both vegetative as well as maturity stages as 11.1 and 15.4,
|| Effect of seed fortification with pulse sprout extracts on
yield attributes of rice variety Co 43
Among the different treatments, the maximum number of productive tillers registered
by horse gram 2% extract at both vegetative (12.4) and maturity stages (19.7)
and it was followed by cowpea extract 4% which recorded 12.1 at vegetative and
18.6 at maturity stages. Total chlorophyll content was significantly influenced
by seed fortification treatment. Among the treatments, horse gram 2% recorded
the highest total chlorophyll content of 39.2 and 28.0 at vegetative and maturity
stage, respectively. The lowest chlorophyll content was recorded by control
(35.8) and (25.8) at both vegetative and maturity stages, respectively. Among
the concentration no significant difference was observed between the concentrations
and the interaction of treatmentxconcentration for chlorophyll content. The
seed fortification treatments varied significantly from each other. According
to Table 3 among the treatments, horse gram 2% recorded the
maximum panicle length (22.4 cm) which was on par with cowpea 4% (22.3 cm).
They were followed by horse gram 3% (21.8 cm) and cowpea 4% (21.6 cm). The minimum
panicle length was observed in control recorded (20.5 cm). Significant variations
were observed in number of seeds per panicle due to the seed fortification treatment.
Among the treatments horse gram 2% recorded the maximum number of seeds 163.2,
it was on par with cowpea 4% (162.7) followed by horse gram 3% (125.2) and cowpea
5% (123.4). They were on par with each other. The minimum number of seeds panicle-1
was observed in control recorded (87.3). Significant differences were observed
among the treatments. The highest value was recorded by horse gram 2% (19.6
g) which was on par with cowpea 4% (19.5 g), it was followed by horse gram 3%
(18.6 g). The minimum value recorded by control (16.7 g). A significant variation
in single plant yield was observed due to seed fortification treatment. Among
the treatments, horse gram 2% recorded the maximum seed yield (12.2 g), followed
by cowpea 4% (11.3 g). The lowest seed yield recorded by control (10.4 g). Significant
results were obtained for seed yield sq.m-1. Among the treatments,
horse gram 2% recorded the highest seed yield (395 g), followed by cowpea 4%
(370 g). The lowest seed yield recorded by control (342 g). Significant difference
was found in seed yield plot-1. Among the treatments, horse gram
2% recorded the maximum seed yield (6.32 kg). The minimum seed yield recorded
by control (5.48 kg). Highly significant variation was observed in seed yield
ha-1. Among the treatments, horse gram 2% recorded the highest seed
yield (3951 kg) while the lowest seed yield recorded by control (3426 kg).
The results revealed that irrespective of the pulse sprout all the treatments
recorded better performance compared to control. The control seed recorded the
lowest field emergence of 61 while horse gram 2, 3% and cowpea 4 and 5% pulse
sprout extract 81, 79, 76 and 79, respectively. This may be due to nutritional
quality increased in pulse sprouts which is used for seed fortification. Augustin
and Klein (1989) reported that the content of phosphorous, potassium, zinc
and copper increased significantly as result of germination in various legumes.
Similar results was reported by Martin-Cabrejas et al.
(2007) the remarkable increase in sodium content which increases the nutritional
qualities of the sprouted legumes.
Seed fortification with micronutrients viz., manganese sulphate, ferrous sulphate,
zinc sulphate, ammonium chloride, ammonium molybdate, potassium chloride, potassium
dihydrogen phosphate, magnesium sulphate, borax and ammonium sulphate in three
concentrations viz., 0.5, 0.75 and 1.0% were proved to be better than control
in terms of speed of germination, germination percent, root length (cm), shoot
length (cm), dry matter production (g seedlings-10) as well as vigour
index (Marimuthu, 2007). Grzywnowicz-Gazda
(1982) reported that soaking of spring barley seeds in B, Mn, Mo, Zn, Fe,
Mg either individually or in mixture for 24 h increased the trace element concentration
in the grain. The seed treatment was also found to increase the germination
capacity, growth and dry matter production of barley seedlings when compared
with untreated control. Nitrogen containing compound might have stimulated the
germination by increasing the seed cytokinin content, occurring naturally in
seeds which interacted with growth inhibitors and enhance the metabolic process,
leading to higher germination (Khan, 1980). FaShui
et al. (1996) noticed the increased germination rate, seed vigour,
seedling fresh weight, seedling height and root length when maize seeds were
soaked in solution of 0.3% CaCl2, 0.1 per cent ZnSO4 either
individually or in combination.
Seed fortification with molybdenum as a Sodium molybdate, Zinc sulphate and
Manganese sulphate @ 0.1, 0.2 and 0.2%, respectively, recorded maximum seed
physiological quality attributes in laboratory (Natesan,
2006). The sulphur in zinc sulphate given as fortification was found to
increase the level of vitamins, biotins and thiamins and its coenzymes (Srimathi
and Malarkodi, 2000). The overall performance of the treatments underscore
that manganese sulphate (1.0%), ferrous sulphate (0.75%) and ammonium molybdate
(0.75%) can well serve as seed fortification agent to increase the seed vigour
of rice seeds.
During seed fortification, the first phase of germination ends with completion
of imbibition process and hence, the time taken from sowing to emergence is
much reduced reported by Hegarty (1970). Fortification
of seed increased the field germination of corn by promoting embryo growth (Zubenko,
1959). The improvement in field emergence due to fortification could also
be ascribed to activation of cells which results in the enhancement of mitochondrial
activity leading to the formation of more high energy compounds and vital biomolecules
which were made available during the early phase of germination observed by
Dharmalingam et al. (1988). These initial changes
culminate in enlargement of the latent embryo. The probable reason for higher
germination in fortified treatment could be due to greater hydration of colloids,
higher viscosity and elasticity of protoplasm, increase in bound water content,
lower water deficit, more efficient root system (May et
al., 1962) and increased metabolic activity (Joseph
and Nair, 1989). It might also be due to enhanced metabolic activity resulted
in early germination as stated by Joseph and Nair (1991).
Studies. Thus it is obvious that, the presence of bioacitve substances in sprouted
horse gram and cowpea extracts viz., amino acid, vitamins and minerals could
have resulted in fortification of rice seeds as corroborated by earlier reports.
The seed fortification treatments influenced the plant height both at vegetative and maturity stages. The maximum plant height was recorded by horse gram 2% which recorded 83.1 and 101.8 cm in vegetative and maturity stages which was followed by 5% cowpea extract which recorded 82 and 100 cm, respectively. Similarly the number of tillers and productive tillers recorded at both the vegetative and maturity stages ascertained that seed treatment with both horse gram and cowpea had augmenter effects on the growth and productivity of rice plants. The increment recorded was maximum with horse gram 2% extract which recorded 12 and 28% over control at vegetative and maturity stages, respectively.
The chlorophyll content of the plants grown out of fortified seeds were estimated both at vegetative and maturity stages. The seed fortification with 2% horse gram extract was found to boost the chlorophyll content of the plants to the highest level at both vegetative and maturity stages. The increase recorded over control was 9 and 8% over control, respectively. It was closely followed by cowpea 5% extract which could improve the chlorophyll content up to 8% over control in both stages.
The effect of the seed fortification treatments with pulse sprout extracts were found to be profound on the yield attributing factors viz., panicle length (cm), number of seeds per panicle and 1000 seed weight. The control plants could record the lowest values for panicle length (20.5 cm), number of seeds per panicle (87.3) as well as 1000 seed weight. (16.7 g). from these values, the seed fortification with horse gram 3% extract could improve the values to 22.4 cm, 163.2 and 19.6 g, it was followed by 5% cowpea extract which recorded 22.3, 162.7 and 19.5 g which were at par with each other.
The improvement with yield attributing factors that were enabled by seed fortification with pulse sprout extract obviously resulted in higher seed yield. The seed yield were analysed at single plant and per square meter and computed to per plot and per hectare. The control seeds recorded the lowest levels of 10.4, 342 g, 5.48 and 3426 kg at plant -1, square meter-1, plot-1 and hactare-1. The horse gram 2% extract recorded 12.2, 395, 6.32 and 395, respectively. The improvement was upto 17, 16, 15 and 15% over control, respectively. Similarly, cowpea 5% extract could record 9, 8, 8 and 8% over control.
The seed vigour extended due to fortification of rice seeds with pulse sprout
extracts had resulted in better plant growth as reflected in plant height, increased
chlorophyll content and higher number of productive tillers. Besides the growth
factors the yield attributing factors viz., panicle length (cm), number of seeds
per panicle and 1000 seed weight were also found to improved. This could have
been due to higher photosynthetic rate enable by the high chlorophyll content
recorded in the plants raised from the pulse sprout extract fortified seed.
The seed yield increase in finger millet was attributed to maintenance of high
leaf area index and chlorophyll stability index and less proline content by
Kalarani et al. (2001). Seed yield increase in
rice was supported by the favorable high value for the physiological parameters
like germination, field emergence, vigour index, total dry matter production,
chlorophyll stability index and proline content at different stages of crop
growth (Thandapani and Subarayalu, 1986). This might
be due to the combined effect of fortification and coating which might have
enhanced the nutrient uptake and thereby increased the dry matter production.
The increase in dry matter production due to the above said treatment in the
present study is in accordance with the report of Periyathambi
and Palaniyappan (1981) and Selvaraju (1992) in sorghum
and Rangasamy et al. (1993) in agricultural crops.
The field experiment was laid with rice seeds fortified with cloth bag squeezed extract of horse gram @ 2 and 3% and cowpea 4 and 5% where dry seeds served as control. The maximum plant height was recorded by horse gram 2% which recorded 83.1 and 101.8 cm in vegetative and maturity stages which was followed by 5% cowpea extract which recorded 82 and 100 cm, respectively. Similarly the number of tillers and productive tillers recorded at both the vegetative and maturity stages ascertained that seed treatment with both horse gram and cowpea had augmenter effects on the growth and productivity of rice plants. The seed fortification with 2% horse gram extract was found to boost the chlorophyll content of the plants to the highest level at both vegetative and maturity stages. The control seeds recorded the lowest seed yield levels of 10.4, 342 g and 3426 kg at plant -1, square meter-1and hactare-1, respectively while the horse gram 2% extract recorded 12.2, 395, 6.32 and 395, respectively.
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