Effect of Remediation on Growth Parameters, Grain and Dry Matter Yield of Soybean (Glycine max) in Crude Oil Polluted Soils in Ogoni Land, South Eastern Nigeria
The effectiveness of organic and inorganic fertilizers (cow dung, poultry manure, NPK and municipal waste compost) in the remediation of crude oil polluted soil in Ogoni, Rivers state was assessed using soybean as a test crop. A simple factorial field experiment arranged into a randomized complete block design with four replicates and twenty-four experimental plot with six treatments was used. Plot treated with poultry manure recorded the highest value of emergence (79 and 74.62%) at both seasons followed by NPK (73 and 68.32%), Cow dung (69.37 and 67.92%), municipal waste compost (66.25 and 65.3%), respectively. The least emergence (56.50 and 60%) was observed in the control for both seasons. Soybean plants height were highest in plot treated with poultry manure 4 and 6 WAP (11.48 and 12.70 cm) and (24.70 and 19.18 cm) for both seasons. There was decreased in the following order NPK (10.98 and 11.58 cm) and (23.28 and 17.05 cm), cow dung (10.18 and 11.96 cm) and (20.88 and 17.10 cm), municipal waste compost (10.03 and 11.08 cm) and (18.58 and 16.88 cm), No pollution plot (9.80 and 10.65 cm) and (16.43 and 16.70 cm) and then the control (polluted with no amendment) (8.65 and 9.33 cm) and (14.85 and 14.50 cm). NPK had the largest leaf area (12.95 and 12.70 cm2) and (14.43 and 14.56 cm2) 4 and 6WAP for both seasons, followed by poultry manure (11.80 and 11.93 cm2) and (13.72 and 12.8 cm2), municipal waste compost (12.29 and 11.53 cm2) and (12.93 and 12.49 cm2), cow dung (11.45 and 11.13 cm2) and (13.06 and 12.51 cm2), respectively. Poultry manure gave the highest grain yield per hectare (2,800 and 3,875 kg ha-1) for both seasons followed by NPK (2,220 and 2,687 kg ha-1), municipal waste compost (2,000 and 2,190 kg ha-1) and no pollution (1,575 and 1,780 kg ha-1) for both season. NPK had the highest dry matter yield at both season (8,500 and 8,000 kg ha-1), respectively. This was followed by poultry manure (7,000 and 7,500 kg ha-1), cow dung (6,000 and 7,000 kg ha-1), municipal waste compost (5,500 and 4,500 kg ha-1) and no pollution (3,000 and 2,500 kg ha-1), respectively. The least dry matter yield was observed in the control (2,500 and 2,000 kg ha-1) for both seasons. There was improvement in the dry matter yield value of poultry manure (7,500 kg ha-1) and cow dung (7,000 kg ha-1) at late planting when compared to that of early planting. Since, there were no significant differences in leaf area between NPK, poultry manure and cow dung treatments, the results indicate poultry manure as a remediation material in crude oil polluted soils planted to soybean.
Received: November 10, 2011;
Accepted: January 16, 2012;
Published: February 23, 2012
Remediation practices are those treatments or technologies used to restore
a polluted or contaminated soil and water (Odu, 2006).
Remediation is very necessary in Ogoniland as a result of crude oil spillage
a regular occurrence in the area (Legbosi, 2005). Crude
oil spillage is a regular occurrence in the Niger Delta region of Nigeria where
over 80% of the crude oil is produced. Dublin-Green et
al. (1998) reported that from 1979-1997, the Nigeria petroleum industries
spilled 5334 barrels of oil into the Niger Delta.
Ogoniland is located in the south-eastern part of the lower Niger Delta region
and is endowed with abundant natural resources including oil and gas. Ogoniland
thus, plays host to five flow stations and 96 oil wells (Vikoo,
2003). Despite the suspension of oil exploration and exploitation activities
in Ogoniland since the year 1993, the area is yet to be spared from oil spillage
due to the presence of oil pipeline cris-crossing the entire Ogoni landscape
and some of which are trans-Niger tank lines that transport oil to shipment
terminal in Bonny Island. No other post-spill remediation responses have been
embarked on beyond extinguishing the fire and plastering of the leaking pipes
(Hart, 1997). There is need for remediation of the coastal
plain sands of Ogoniland using less expensive and easily affordable remediation
material such as cow dung, poultry manure, municipal waste compost with soybean
as a test crop. Some research workers have investigated the effect of crude
oil pollution on soils and crop growth (Rowell, 1977; Kinako,
1981; Amakiri and Onofeghara, 1983; Amadi
et al., 1992; Ebie, 2006). Those researches
were carried out in the temperate region, Sambriemo-warri Deltaic plain geomorphic
zone and the Meander belt or Rivers and Bayelsa State (Snowden
and Ikweozor, 1987). Ayolagha et al. (1994)
also carried out research on remediation of crude oil polluted soils using municipal
waste compost for soybean production in Niger Delta, but this experiment was
carried out in a green house which was a limiting factor because, the grains
and dry matter yield of soybean after harvest under natural conditions which
is important to farmers was not assessed.
Soybean as a leguminous plant has a symbiotic relationship with microorganisms
within the soil especially the symbiotic nitrogen fixing bacteria which promote
plant growth and development (Amakiri, 2000).
Soybean has been recognized as a leading tropical grain legume which provide
cheap and balanced diet (Ogundipe et al., 1989)
with proximate analysis of 40% protein, 32% carbohydrate, 20% edible oil, 5%
mineral and 3% fibre.
Application of N to soybean in the tropic has given variable result depending
on such factors as rhizobial strains (Harder-Son et al.,
1987). But no information has been said on the effect of crude oil polluted
soil on soybean in Ogoniland (coastal plain sand) under natural field conditions.
The objective of this research was to investigate the effect of remediation
materials on growth parameters of soybean planted to crude oil polluted soils
of the coastal plain sands of Ogoniland, Rivers State.
MATERIALS AND METHODS
The experiment was conducted on a farmers field in Baa-Lueku in Nyokhana District of Khana local Government Area of Rivers State. It could be located at the following georgraphical co-ordenate, (44051N and 7°22 53. 7E and 4°43 19.5N and 70 27°9.8E).
The soil of the study area is the well-drained costal plain sand deposit (Ultisol). The experimental site has been under fallow after some years of intensive cultivation of maize, okra, yam, cocoyam, cassava, pumpkin and garden egg without history of soybean cultivation.
The rainfall distribution of the study area ranges from 2000-3000 mm per annum in a bimodal form with average temperature of about 28-35°C depending on the season of the year.
The experiment involves six treatments with four replicates. These gave a total
of twenty four plots in randomized complete block design. Each plot of 2x2 m
sizes was polluted with 2.4 L of fresh Bonny light crude oil with specific gravity
of 0.835 which is equivalent to one percent pollution (Elf,
2000). The no pollution and control plots were not polluted. The crude oil
was measured into watering can spread on each plot and allow to fallow for two
weeks before application of remediation materials. The remediation materials
used were cow dung, poultry manure, NPK and municipal waste compost. The rate
of application were cow dung 10,000 kg ha-1, poultry manure 10,000
kg ha-1, NPK 250 kg ha-1 and municipal waste compost 10,000
kg ha-1 in that order.
Two weeks after crude oil pollution of each plot, remediation materials were carefully applied except the control and no pollution plots. The remediation materials were tilled into the soil after being spread and left fallow for another two weeks before planting. After four weeks of planting, the second round of remediation materials were applied in the plots using the ring method except the control and no pollution plots.
Percentage emergence 7 days after planting (7DAP): Percentage emergence of soybean plant was taken 7 days after planting (7DAP). Emergence counts was determined by the formula:
Plant height 4 and 6 weeks after planting (4 and 6 WAP): Plant height per plot was taken at 4 and 6 weeks after planting. The height of soybean plants per plot was measured using measuring tape from the base of the plant to the collar of the last leaf in the plant.
Leaf area of soybean plant (Glycine max) 4 and 6 weeks after planting:
The leaf area of soybean plant 4 and 6 weeks after planting was determined using
the punch techniques described by Osodeke and Ojenije (2003).
Five leaves were taken from the total leaves per plot and punched. The punched
leaves were weighted. The fresh weight of the total leaves per plot was also
taken for analysis to relate weight with length using the formula:
Grain and dry matter yield 12 weeks after planting: Twelve week after planting the grain yield was assessed by weighing the dry grains in kg ha-1. The dry matter yield was assessed by oven drying the whole plant at 80°C for 48 h to a moisture content of 14% and determining the dry weight using top weighing balance.
All data from the field were analyzed using statistical procedure for the analysis
of variance (ANOVA) (Steel and Torrie, 1960). The new
Duncan (1955) multiple range test (NDMRT) and SAS
(1999) was used to compare the mean and to analyze the Least Significant
RESULTS AND DISCUSSION
Effect of remediation materials on emergence of soybean plant seven (7)
days after planting (7DAP): The number of soybean plant that emerged at
7 DAP is presented in Table 1. Soybean emergence was highest
in crude oil polluted soil treated with poultry manure (79 and 74%) and decreased
in the following order NPK (73 and 68%), Cow dung (69 and 67%), municipal waste
compost (66 and 65%) and no pollution (65 and 64%). The control pollution with
no amendment was (56 and 60%). Low seedling emergence in the control was because
crude oil soaked some of the seed and damaged them. This is in support of the
findings of Kalio (2003) that for a seed to germinate
effectively, it must be viable or alive. The soaked cotyledons or embryo by
crude oil leads to poor germination.
This also support the finding of Horne (1978) which stated
that heavy crude oil pollution lead to poor germination due to poor soil aeration.
He pointed out that this increase soil temperature leading to poor seedling
The highest seedling emergence in plot treated with poultry manure and NPK
was as a result of the fact that they are easily dissolvable and absorbable
(Awodun, 2007). They added that this promote microbial
degradation of hydrocarbon because poultry had a lot of micro organisms and
at the same time help to bind the soil particles to improve the soil structure
thereby improving the soil water and temperature regimes. Early planting had
highest seedling emergence than late planting to support the finding of Ebie
(2006) that high rainfall at late planting season affect the rate of seed
germination. However, the mean value for both seasons were not significantly
different from one another at p<0.05.
Effect of remediation on soybean plant height 4 and 6 weeks after planting:
The result of remediation on soybean plant height is presented in Fig.
1. Result showed that poultry manure has significant veridical growth measurement
across the different seasons in conformity with the report of Alasiri
and Ogunkeyede (1999) and Awodun (2007) which stated
that poultry manure is easily dissolvable and absorbable as such soybean quickly
absorbed the nutrient leading to fast growth rate. Poultry manure gave plant
height of (11.48 and 12.70 cm) 4 weeks after planting at early and late planting
and (24.70 and 19.18 cm) 6 weeks after planting at early and late planting respectively.
This was followed by NPK (10.98 and 11.58 cm) 4 WAP at both early and late planting
and (23.28 and 17.05 cm) 6 WAP for both early and late planting; followed by
cow dung, municipal waste compost and no pollution in that order. The least
vertical growth measurement was recorded in the control plot.
|| Effect of remediation materials on emergence of soybean plant
||Effects of remediation on soybeans plant height. EP: Early
planting, LP: Late planting, WAP: Weeks after planting, NPK: Nitrogen, phosphorus
and potassium fertilizer, MWC: Municipal waste compost
||Effects of remediation on soybeans leaf area, EP: Early planting,
LP: Late planting, WAP: Weeks after planting, NPK: Nitrogen, phosphorus
and potassium fertilizer, MWC: Municipal waste compost
Plants were higher were 6 weeks than at 4 weeks after planting due to climatic
advantages. The higher plants observed in plots treated with poultry manures
and NPK was because they were in simple dissolvable and absorbable form, that
promotes easy plant uptake for their growth (Awodun, 2007).
He reported that poultry manure contain a lot of microorganism which help to
degrade the crude oil and enhance plant growth.
At late planting 4 weeks after planting, the growth rate of soybean in no pollution
plot improved to (10.65 cm) because non application of remediation material
increased the rate of nodulation to enhance Nitrogen fixation in conformity
with the findings of other researchers (Amakiri, 2000;
Singh and Saxena, 1977; Mansimba
and Mondibaye, 1996). The increase in root nodules could also be caused
by their ability to adopt to the environmental stress created by the non-application
of remediation materials. This is in line with the observation of Giller
and Wilson (1993) and Chinke et al. (2000)
which stated that the Rhizobia species have the ability to adapt to the environmental
Effect of remediation on leaf area of soybean plant: The results of
the effect of remediation on crude oil polluted soils as indicated by leaf area
of soybean are represented in Fig. 2.
||Effect of Remediation on soybean grain yield per hectare.
EP = Early Planting, LP = Late Planting, NPK = Nitrogen, phosphorus and
potassium fertilize, Mwc = Municipal waste compost
The result showed that leaf area generally was highest in NPK Plot followed
by that of poultry manure because their nutrients were probably released faster
and absorbed by plant (Awodun, 2007). The general trend
in a decreasing order is as followed, NPK, Poultry manure, municipal waste compost,
cow dung, no pollution and control plot both in early and late plantings at
4 and 6 weeks after planting. The leaf area at 4 WAP ranged from 9.24 o 12.96
cm2 for NPK during early planting 8.92 cm2 for control
to 12.70 cm2 for NPK at late planting. The leaf area at 6 WAP ranged
from 10.48 cm2 for control to 14.43 cm2 for NPK at early
planting and 10.69 cm2 for no pollution plot to 14.56 cm2
for NPK at late planting, respectively.
Effect of remediation on soybean grain yield in kilogram per hectare (kg ha-1): The result on soybean grain yield for hectare is presented in Fig. 3. The results showed that at early planting poultry manure had the highest grain yield per hectare (2,800 kg ha-1). This was followed by NPK fertilizer (2,220 kg ha-1), municipal waste compost (2,000 kg ha-1), cow dung (1,631 kg ha-1) and No pollution (1,575 kg ha-1), respectively.
The lowest grain yield per hectare (1,044 kg ha-1) was observed in the control. The easily dissolvable and absorbable form of poultry manure and NPK which promotes other growth parameters also enhanced increase in yield at early planting. During late planting, poultry manure also had the highest grain yield of (3,875 kg ha-1) followed by NPK (2,687 kg ha-1) and municipal waste compost (2,190 kg ha-1). Cow dung and no pollution plots had similar grain yield of (2,000 and 1,780 kg ha-1), respectively.
However, there were significantly different from one another at p<0.05. The control had the lowest grain yield of 1,500 kg ha-1. The lowest grain yield observed in the control plot was as a result of the non application of remediation materials.
This agrees with Raymond et al. (1976) that
crude oil has negative effect on the soil because of the carbon content. Increase
soil organic carbon is detrimental to plant growth and development. It was also
observed that the highest grain yield per hectare for poultry manure at late
planting was as result of it ability to bind soil particles to form a good soil
structure to retain moisture at late planting.
When compare with the findings of Ebie (2006) on maize
grain yield, it was discover that poultry manure had (2,039 and 2,109 kg ha-1)
at early and late planting seasons, respectively followed by cow dung (510 and
1,615 kg ha-1) and NPK (1,500 kg ha-1).
||Effects of Remediation on soybean Dry matter yield in kilogram
per hectare (kg ha-1). EP = Early Planting, LP = Late Planting,
NPK = Nitrogen, phosphorus and potassium fertilizer, Mwc = municipal waste
This showed that soybean had better grain yield in crude oil polluted soils amended with similar remediation materials than maize.
It was also observed that Ebie (2006) worked on maize
in the Meander Belt geomorphic regions of Bayelsa State, while Peter
(2009) worked on soybean planted to crude oil polluted soil in coastal plain
sand of Ogoni all in Niger Delta.
Effect of remediation on soybean dry matter in kilogram per hectare (kg
ha-1): The effect of the various remediation materials on soybean
dry matter is shown in Fig. 4. It showed that soybean plant
treated with NPK had the highest dry matter yield of (8,500 and 8,000 kg ha-1)
at both early and late planting seasons and NPK is in a form that is easily
dissolvable and absorbable, thereby enhance quick up take for proper growth
and development in plant. Poultry manure had (7,000 and 7,500 kg ha-1)
followed by cow dung (6,000 and 7,000 kg ha-1) and municipal waste
compost (5,500 and 4,500 kg ha-1) at early and late planting, respectively.
These findings were compare with Ebie (2006) re on maize
dry matter and it was observed that poultry manure gave the highest dry matter
of (13,187 and 12,969 kg ha-1) followed by cow-dung (11,145 and 10,052
kg ha-1) and NPK (8,229 and 7,239. 5 kg ha-1) for early
and late seasons, respectively.
The higher dry matter in maize plants than soybean may be as a result of the
structural or morphological differences in both plants (maize and soybean).
It was also observed that poultry manure gave the highest dry matter yield in
maize (Ebie, 2006). While NPK gave the highest dry matter
yield in soybean across seasons. The dry matter of soybean treated with poultry
manure improved to 7,500 kg ha-1 at late planting due to climatic
advantage and it ability to bind soil particles to form a good soil structure.
The higher moisture retentive capacity of poultry manure might have also contributed
to the improved dry matter yield at late planting irrespective of the treatments
dry matter yield for both seasons (early and late planting).
The various remediation materials used for this study improved the different growth parameters of soybean such as seeding emergence 7 DAP, plant height and leaf area 4 and 6 week after planting at both early and late planting. Generally, the highest performance in term of percentage emergence and plant height was recorded in plot treated with poultry manure, followed by NPK, Cow dung and municipal waste compost. NPK had the highest leaf area in both seasons the least growth parameters were recorded in the No pollution and controls. These show that the remediation materials have positive effect on crude oil polluted soil planted to soybean in Ogoniland, Rivers State.
I am thankful to Chief Hon. Dumnamene Dekor (Mene Yereba 1) of Bua Bangha and Deputy speaker Rivers State House of Assembly for funding this study.
I am also grateful to David Nwisuator for the analytical assistance provided during the course of the study.
Alasiri, K.O. and S.A. Ogunkeyede, 1999. Effect of different levels of poultry manure on seed yield of okra. Soil Sci. Soc., 25: 102-102.
Amadi, A., A.A. Dickson and G.O. Maater, 1992. Remediation of polluted Soil: Effect of organic and inorganic nutrient on soil supplements on the performance of maize water. Air Soil Pollut., 65: 59-76.
Amakiri, J.O. and F.A. Onofeghara, 1983. Effect of crude oil pollution on the growth of Zea mays, Abelmoschus esculentus and Capsicum frutescens. Oil Petrochem. Pollut., 1: 199-205.
Amakiri, M.A., 2000. Microbes moving the world forward in the new millennium. Rivers State University of Science and Technology, Nkpolu-Oroworukwu, Port Harcourt, November 29, 2000.
Awodun, M.A., 2007. Effect of poultry manure on the growth, yield and nutrient content of fluted pumpkin (Telfaria occidentalis Hook F). Asian J. Agric. Res., 1: 67-73.
CrossRef | Direct Link |
Ayolagha, G.A., F.S. Kio-jack and N.O. Isirimah, 1994. Remediation ofcrude oil polluted soils using municipal waste compost for soybean production in niger delta. Proceedings of the 23rd Annual Conference of Soil Science Society of Nigeria, (SSSN'94), Congregation Hall, City Campus -.
Chinke, N.M., E.N. Iuafor, O.N. Sanginga, and V.O. Chude, 2000. Influence of phosphorus and molybdenum on the infection of cowpea by striga. Nigeria J. Soil Sci., 12: 58-66.
Dublin-Green, W.F. Nwankwo, J.N. and D.O. Irechukwu, 1998. Effective regulation and management of HSE Issues in the petroleum History. Proceedings of the SPE International Conference on Health Safety and Environment in Oil and Gas Exploration and Production, June 7-10, 1998, Caracas, Venezuara -.
Duncan, D.B., 1955. Multiple range and multiple F tests. Biometrics, 11: 1-42.
CrossRef | Direct Link |
Ebie S.J., 2006. Remediation of crude oil polluted soil using organic and inorganic fertilizers for maize product in Yenagoa, Bayelsa State. M.Sc. Thesis, Department of Crop/Soil Science Faculty of Agriculture, Rivers State University of Science and Technology Port Harcourt, Nigeria.
Elf, 2000. Percentage pollution derivation form crude oil, Obagi Elf operation area OML. Institute of Pollution Studies, Rivers State University and Science and Technology, Port Harcourt.
Giller, K.E. and K.J. Wilson, 1993. Nitrogen Fixation in Tropical Cropping Systems. CAB International, Wallingford, USA., ISBN: 9780851988429, Pages: 313.
Harder-Son, G., S.K.A. Danson and F. Zapata, 1987. Biological Nitrogen Fixation in Field Crops. In: Hand Book of Plant Science in Agriculture, Christie, B.R. (Ed.). CRC Press Inc., Boca Raton, USA., pp: 165-192.
Hart, S.A., 1997. Effect of crude oil pollution on the niger delta. M.Sc. Thesis, Department of Zoology, University of Port Harcourt, Nigeria.
Horne, R.A., 1978. The Chemistry of our Environment. John Wiley and Sons, New York,USA., ISBN-9: 9780471409441, Pages: 869.
Kalio, D., 2003. The toxicity impact of oil spills on biodiversity. Proceeding of the International Seminar on The Petroleum Industry and the Nigeria Environment, (PINE'2003), Nigeria, pp: 171-174.
Kinako, P.D.S., 1981. Short-term effects of oil pollution on species numbers and productivity of a simple terrestrial ecosystem. Environ. Pollut. Series A: Ecol. Biol., 26: 87-91.
CrossRef | Direct Link |
Legbosi, S.P., 2005. The Impact of Oil Spills on Biological Diversity. Sarobery Publisher, Port Harcourt, Nigeria, pp: 8-26.
Mansimba, G.R. and R. Mondibaye, 1996. Effect of inoculation and n-fertilization on soybean grown on congo soil. Biol. Agric. Horticult., 13: 197-204.
Odu, C.T.I., 2006. Environmental degradation, restoration of pollution sites: Desert and desertification modified to environment state government. Ministry of Environment, Port Harcourt Rivers State, Nigeria.
Ogundipe, H.O., K.E. Dashell and S.M. Osho, 1989. Soymilk yield and quality as affected by soybean varieties and processing technique. Tropical Legumes Bulletin No. 38
Osodeke, V.E. and S.O. Ojenije, 2003. Nitrogen requirement of some promiscuous soybean cultivars in acid soil of the forest zone of South Eeatern Nigeria. Nigeria J. Soil Sci., 12: 2-5.
Peter, K.D., 2009. Remediation of crude oil polluted soils using organic manures and NPK fertilizer for soybean production on ogoni sands, Rivers State. M.Sc. Thesis, Department of Crop/Soil Science Rivers State University of Science and Technology Port Harcourt, Nigeria.
Raymond, R.L., J.O. Audson and V.W. Jamison, 1976. Oil degradation in soil. Applied Environ. Microbiol., 31: 522-535.
PubMed | Direct Link |
Rowell, M.J., 1977. The Effect of Crude Oil Spills on Soils. A Review of Literature. In: The Reclamation of Agricultural Soils after Oil Spills, Toogood, J.A. (Ed.). University of Alberta, Edmonton, pp: 1-33.
SAS, 1999. SAS/STAT User's Guide. 4th Edn, Vol. 2, Version 6, SAS, Cary, New Jersey, USA.
Singh, B.B. and M.C. Saxena, 1977. Phosphorus fertilization of soybean. Indian J. Agric. Sci., 43: 925-929.
Snowden, R.J. and I.K.E. Ikweozor, 1987. The impact of minor oil spillage in the estuarine Niger delta. Marine Pollut. Bull., 18: 595-599.
CrossRef | Direct Link |
Steel, R.G.D. and J.H. Torrie, 1960. Principle and Procedure of Statistic. MGGraw-Hill Book Co. Inc., New York, Toronto, London Pages: 312.
Vikoo, B., 2003. The Mogho people and the development of modern Ogoni nation. Spring Field, Owerri, Nigeria Paper 1