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Research Article
 

Factors Regulating Composition and Abundance of Phytoplankton in El Dekhaila Harbor, South-Eastern Mediterranean Sea, Egypt



Hanan Mohamed Khairy and Samiha Mahmoud Gharib
 
ABSTRACT

Background and Objective: The abundance and distribution of phytoplankton in harbors exhibits spatial and temporal variation. Many biotic and abiotic factors interact to regulate spatial and temporal variations in phytoplankton. This study was carried out to determine which factors can regulate the abundance and community structure of phytoplankton in El Dekhaila Harbor, South-Eastern Mediterranean Sea, Egypt. Materials and Methods: Samples of phytoplankton were gathered during 2012 in El Dekhaila Harbour, for statistical tests three indices were utilized to get the estimation of species diversity (H’), species richness (d) and species evenness (J) and also Pearson’s Correlation Coefficient (r) was utilized for the simple relationship analysis with the variables. Results: Phytoplankton groups were essentially made out of Bacillariophyceae (84.58%) and also, have been determined as the richest group in species variety with 72 taxa. Summer had higher phytoplankton density, however autumn had higher richness and evenness. Skeletonema costatum, Pseudo-nitzschia seriata, Chaetoceros curvisetus and Prorocentrum spp. were frequently recorded. El Dekhaila Harbor suffered of high concentrations of both inorganic nitrogen and dissolved reactive phosphate. Conclusion: Zooplankton grazing activity on phytoplankton may assume a critical factor in regulating the phytoplankton community structure in El Dekhaila Harbor and not the concentrations of nutrient salts or their ratios.

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Hanan Mohamed Khairy and Samiha Mahmoud Gharib, 2017. Factors Regulating Composition and Abundance of Phytoplankton in El Dekhaila Harbor, South-Eastern Mediterranean Sea, Egypt. Asian Journal of Biological Sciences, 10: 27-37.

DOI: 10.3923/ajbs.2017.27.37

URL: https://scialert.net/abstract/?doi=ajbs.2017.27.37
 
Received: January 14, 2017; Accepted: February 28, 2017; Published: March 15, 2017

INTRODUCTION

Pollution in marine environments had turned out to be one of the troublesome problems and it has been developed wherever all through the system of the marine natural life. A large portion of the projects on the planet earth are settled in the water bodies of the countries, particularly, around significant commercial harbors because of simplicity of exchange, which adds to serious pollution.

Phytoplankton community structures are extremely complex qualitatively and quantitatively. Succession shifts in phytoplankton community structure are basically a result of changes in ecological factors (e.g., type, concentration and of nutrient salts limitation) as well as possibly moves in higher trophic levels (e.g., zooplankton)1-4. Recognizing the natural variables that manage phytoplankton community structure is essential for encouraging the elaboration of wide theories of pervasive ecological issues, for instance, eutrophication and harmful algal blooms5,6.

El-Mex Bay is located in the southeastern side of the Mediterranean at longitude 29°45' and 29°54' E and latitude 31°07' and 31°15' N, with an average depth around 10 m and surface area of around 19.4 km2. The bay is a very dynamic system and of extraordinary natural, since it receives a huge amount of agricultural, industrial and sewage wastes from the nearest Lake Mariut through El-Umoum Drain amounted 2.547×109 m3 y–17. The discharged water input impact on all the water life, particularly the phytoplankton community structure.

El Dekhaila Harbor is located on the western side of El-Mex Bay and is considered one of the most famous commercial harbor in Egypt. The harbor's water is subjected to multisource of wastewaters beginning from El-Mex Bay through El-Umoum Drain8,9, impacted to a great extent by anthropogenic components, which cause inhibition and promotion of the plankton development10,11.

Few studies have been done on the physical and chemical characteristics of El Dekhaila Harbor water8,12,13. Several studies of phytoplankton community structure have been done in El-Mex Bay and the adjacent waters14,15,11. Zaghloul et al.15 studied the phytoplankton in El Dekhaila Harbor and recorded Merismopedia punctata, Ankistrodesmus falcatus, Euglena granulate, Skeletonema costatum, Nitzschia microcephala and Protoperidinium minutum as the overwhelming species in the harbor during 1990-1991.

Ismael and Dorgham11 sdetected the overwhelming species among 107 taxa were Prorocentrum micans, Oscillatoria sp., Cerataulina smithii, Pseudonitzschia sigma, Spirulina sp., Gymnodinium minor, Coscinodiscus radiates, Euglena acus, Prorocentrum minimum, Protoperidinium curvipes, Chlorella c.f. sp., Cyclotella meneghiniana, Scrippsiella trochoidea, Prorocentrum triestinum, Cyclotella striata and Skeletonema costatum.

Ismael and Abdel-Aziz16 studied the relationship between phytoplankton and zooplankton in the harbor. Shaltout and Abd-El-Khalek13 studied the chemical properties, while Heneash17 investigated the zooplankton in the harbor during 2012, parallel to the present work and the collection of samples was at the same time and at same stations.

The objective of this study was to investigate the phytoplankton community structure, including the seasonal variation, species composition and cell densities and distribution. Ecological parameters controlling seasonal variety in the phytoplankton community are discussed, which will give knowledge into the relationships among physical, chemical and biological components in El Dekhaila Harbor.

MATERIALS AND METHODS

El Dekhaila Harbor is a semi-enclosed basin built after 1986. It expects an essential role in the export and import of products, as minerals, metals, manures, salts and grains. It involves the western part of El-Mex Bay (Fig. 1) and has a surface area of about 12.5 km2 and a water depth ranging from 4-20 m with an average of 12.4 m.

Water samples were gathered four times per the year 2012 at nine stations with a Rüttner sampler. The chosen periods relating to winter (January), spring (April), summer (August) and autumn (November). Water samples were collected at a depth of 50 cm at the examined stations; the physicochemical parameters were measured and the results were published by Shaltout and Abd-El-Khalek13 and the zooplankton was also studied and published by Heneash17. The gathered water samples were quickly fixed with 4% formaldehyde for phytoplankton examination. Phytoplankton was counted utilizing 2 mL settling chambers with a Nikon TS 100 inverted microscope at 400X magnification utilizing Utermohl's technique18. Identification of phytoplankton species was carried following previous studies19-25.

A basic mercury thermometer was used to quantify water temperature. Saltiness was measured by a Beckman acceptance salinometer (Model RS-7B).

Statistical analysis: Three indices were used estimate species diversity (H )26, species richness (d)27 and species evenness (J)28. The Pearson's correlation coefficient (r) was utilized for the simple relationship analyses with the variables (N = 36) with SPSS 8.0 Statistical Package Program.

Fig. 1: Area of study and sampling sites

RESULTS

Hydrographic conditions: The assessed chemical parameters were already published by Shaltout and Abd-El-Khalek13. Surface water temperature in El Dekhaila Harbor changed from a minimum of 16.2°C in winter to a maximum of 28.7°C in summer. The surface salinity varied between 28.9 and 35.6 PSU with an average annual of 33.9±SD 1.8 PSU. The lowest values were recorded at station 6 (32.5±2.4) and the highest at station 3 (34.9±1.3). The seasonally average salinity demonstrated significantly lower values than those generally recorded in Egyptian coastal waters (>38 PSU).

Phytoplankton community structure and composition: From the analyzed data, an obvious change in phytoplankton community as to numerical abundance and species composition among stations and in the seasonal cycle. A total of 141 phytoplankton species were quantified through the examination of the 36 samples collected from nine stations in four seasons. Bacillariophyceae made up the highest number (34 genera, 72 species), yet there was a strikingly low number of Dinophyceae (14 genera, 43 species). Freshwater Chlorophyceae, Cyanophyceae and Euglenophyceae were represented by 14, 9 and 3 species, respectively. The most illustrative genera were Navicula (13 species) and Nitzschia (10 species).

Table 1: Taxonomic composition and proportional representation of the phytoplankton groups in El Dekheila Harbor during 2012

Table 2: Taxonomic composition of phytoplankton sampled during 2012 in El Dekhaila Harbor
G: No. of genera, S: No. of species

Bacillariophyceae and Dinophyceae were more abundant both qualitatively (81.56%) and quantitatively (90.66%) than the other taxonomic groups. They were obvious as the two most diverse groups with 51.06 and 30.50% of the total species number, respectively (Table 1).

While Bacillariophyceae was quantitatively the overwhelming division (84.58%), the total number of species on the analyzed stations showed more pronounced varieties at the spatial scale than the temporal one. A high diversity (132 and 131 species) was recorded at stations 1 and 9, while stations 3 and 4 sustained 125 and 126 species, respectively. Approximately comparable numbers of species (120-123 species) were recorded at stations 2, 5, 6 and 7. An obviously smaller number (119 species) was found at station 8. The numbers of phytoplankton species recorded in winter, spring, summer and autumn were 113, 134, 128 and 126, respectively (Table 2) and the maximum number of species in a single sample was 105, at station 1 during summer. Among the recorded species, 103 were perennial; 58 diatoms, 28 Dinophyceae, 5 Cyanophyceae, 9 Chlorophyceae and 3 Euglenophyceae.

Mean phytoplankton density was highest at station 3 (11852 cells mL–1) and least at station 7 (7359 cells mL–1), with an average annual density amounted 9323 cells mL–1. Bacillariophyceae overwhelmed over dinoflagellates at all the harbor examining stations. The bottom-dwelling forms (Pennales) made up 38 species, while the truly planktonic forms (centrales) made up 34 species. The most abundant pennate diatoms recorded were Pseudo-nitzschia seriata (Cleve) H. Peragallo, 1899 (16.17%), Asterionellopsis glacialis (Castracane) Round, 1990 (7.04%) and Pseudo-nitzschia delicatissima (Cleve) Heiden, 1928 (3.25%). The true planktonic diatoms recorded Skeletonema costatum (Greville) Cleve, 1873 (21.07%), Chaetoceros curvisetus Cleve, 1889 Cleve (7.21%), Leptocylindrus danicus Cleve, 1889 (6.67%), Thalassiosira rotula Meunier, 1910 (6.57%) and Cyclotella meneghiniana Kützing (6.36%) as their overwhelming representative during the study time. Prorocentrum micans Ehrenberg, 1834 (16.13%), Prorocentrum triestinum J. Schiller, 1918 (14.66%), Prorocentrum cordatum (Ostenfeld) J.D. Dodge, 1975 (11.90%), Prorocentrum minimum (Pavillard) J. Schiller, 1933 (10.94%) and Protoperidenium minutum (Kofoid) Jørgensen, 1912 (9.08%) were the most abundant heterotrophic dinoflagellate recorded. Chlorophyceans were represented chiefly by Scenedesmus communis E. Hegewald, 1977, Ankistrodesmus falcatus (Corda) Ralfs, 1848 and Chlorella marina Butcher R. W., 1952. Merismopedia Meyen, 1839 and Oscillatoria Vaucher ex Gomont, 1892 dominating the cyanobacterial population and euglenoids typically represent a minor extent of the community and was represented by the genus Euglena Ehrenberg, 1830.

In Shannon-Wiener status, the maximum diversity recorded (H' = 3.387) was found in autumn at station 9, while the minimum (H' = 2.631) was recorded in summer at station 6. The general mean were 2.989±0.108 (winter), 3.064±0.141 (spring), 2.690±0.042 (summer), 3.174±0.107 (autumn). On the other hand, diversity and richness took after a similar pattern at most stations. High diversity was connected with high richness. Evenness demonstrated significant relation with diversity (r = 0.925, p<0.001) and insignificant with richness (r = 0.095, p = 0.582). The increase in richness was connected with increasing salinity (r = 0.526, p<0.05).

The correlation between phytoplankton density and diversity was strongly negative (r = - 0.650, p<0.001) and it is evident that low diversity implies that a stress increases with poor water quality, while the inverse is valid for high diversity results with favorable condition. Species evenness (J) ranged between 0.591 in summer (station 6) and 0.753 in autumn (station 9), with moderately higher values generally recorded during autumn.

Fig. 2(a-d):
Seasonal variations of phytoplankton abundance subdivided by algal groups and species diversity index of El Dekhaila Harbor from winter to autumn 2012 (a) Winter, (b) Spring, (c) Summer and (d) Autumn

On the other hand, diversity and evenness took after a similar pattern at most stations. Testing the diversity-equitability, diversity-species number and diversity richness relationship demonstrated that diversity was extensively uninfluenced by species number (r = 0.197, p = 0.249) and showed a strong significant relation with equitability (r = 0.925, p<0.001). As expected, diversity had a positive relationship with Margalef’s index (r = 0.447, p<0.05).

Seasonal variation of phytoplankton: The average phytoplankton density was 9323±1591 cells mL–1 and the most elevated values were registered in summer and the least values were in autumn. There was a high vacillation in cell density when the temporal distribution of phytoplankton groups was examined. The phytoplankton community comprised mainly of Bacillariophyceae (84.58%) and too much lower extent Dinophyceae (6.08%) (Fig. 2), which exhibited a pronounced increase in autumn (11.02%), while Chlorophyceae, Cyanophyceae and euglenoids collectively fluctuated between 9.9 and 11.3% of the total density.

During winter, the seasonal mean total phytoplankton cell abundance was 6888±1194 cells mL–1, with most significant cell density recorded in station 8, while the least density recorded in station 7. Bacillariophyceae (79.01-90.32%) was the overwhelming division at all the stations. The diatom Skeletonema costatum was a major component (15.33% of the total phytoplankton) followed by Thalassiosira rotula (9.35%), Leptocylindrus danicus (7.85%), Pseudo-nitzschia delicatissima (7.17%) and Chaetoceros curvisetus (6.95%) (Table 3). Dinophyceae exhibited a pronounced percentage at station 9 (17.63%), stations 5 and 8 (6.39%), while Chlorophyceae (2.57-7.63%) was the second group at stations 1, 2, 3, 6 and 7.

Table 3: Top 15 dominant phytoplankton species sampled during 2012 and their percentage to the total phytoplankton in El Dekhaila Harbor

The most significant contributor to the dinflagellates community structure was the genus Prorocentrum with three species. Scenedesmus communis and Ankistrodesmus falcatus were the most green algae observed. Cyanophyceae was rarely recorded, with more persistence of Merismopedia punctata Meyen, 1839 at station 1, while euglenoids achieved least numbers at most stations.

Spatial change of phytoplankton occurred in spring and fluctuated widely as to abundance and predominant species, with a mean achieved 11549±2547 cells mL–1. The total density fluctuated between 8689 cells/ml (station 7) and 15585 cells mL–1 (station 3). Diatoms achieved the most remarkable percentage (81.72-90.19%), while Dinophyceae dropped to 5.43%. The most abundant and frequent species to the total phytoplankton were Skeletonema costatum (15.25%), Pseudo-nitzschia seriata (10.30%), Leptocylindrus danicus (6.89%), Asterionellopsis glacialis (6.62%), Thalassiosira rotula (6.01%), Cyclotella meneghiniana Kützing, 1844 (5.68%) and Chaetoceros curvisetus (5.42%) (Table 3). Dinophyceae was second in importance (3.09-13.97%) at stations 4, 5, 6, 8 and 9, while Chlorophyceae (3.54-7.06%) at stations 1, 2, 3 and 7. The genera Protoperidenium and Prorocentrum from Dinophyceae and Scenedesmus from green algae were frequently noticed.

Phytoplankton flourishes during summer to achieve an average of 14014±2611 cells mL–1. The density ranged between 10518 cells mL–1 (station 9) and 18851 cells mL–1 (station 3). Bacillariophyceae (78.60-86.88%) dominated the harbor at all stations. Skeletonema costatum and Pseudo-nitzschia seriata were the leaders forming 23.99 and 22.26% of the total abundance (Table 3). Running with this summer, a lesser density of dinoflagellates (3.87-5.62%) showed up obviously at stations 3 and 8. Chlorophytes showed up (3.10-7.04%) at stations 1, 2 and 9. The genus Prorocentrum (4 species) and Scenedesmus communis were frequently observed. Cyanophyceans and euglenophyceans constituted all 6.89 % of the total phytoplankton.

The most minimal phytoplankton abundance was seen in autumn (4841±1002 cells mL–1). The percentage of diatoms decreased (79.07%), while the percentage of the dinoflagellates increased (11.02%). Chaetoceros curvisetus (11.68%), Skeletonema costatum (9.62%), Pseudo-nitzschia seriata (9.55%) and Rhizosolenia fragilissima Bergon, 1903 (9.08%) co-dominating the phytoplankton community (Table 3). With the exception of station 1, dinoflagellates were the second most abundant group making up 7.14-27.83% of the total phytoplankton. The genera Prorocentrum was the overwhelming. Chlorophyceae formed 8.66% of the total phytoplankton at station 1, in which Ankistrodesmus falcatus and Scenedesmus communis were frequently recorded. The development of euglenoids achieved the greatest at stations 1 and 2 (7.71 and 6.22%, respectively). Cyanophyceae significantly observed at station 8 (2.21% of the total phytoplankton). The percentage composition of Chlorophyceae at station 1 was extensively higher than different stations and generally second in important during the year round.

Phytoplankton community in relation to physico-chemical characteristics: Spearman rank correlation analyses were performed on environmental parameters and phytoplankton groups in order to research significant relationships. Salinity variations in El Dekhaila Harbor (28.89-36.46 PSU) impact on behavior characters of the other bio-physicochemical variations because of the terrestrial releases originating from Umoum Drain loaded by domestic, farming and mechanical waste water and furthermore by global passing exercises by vessels. For example, correlations between salinity variations and other physicochemical parameters demonstrated significant correlations, as dissolved oxygen (r = -0.356, p<0.05), dissolved phosphate (- 0.445, p<0.05) and pH (r = 0.343, p<0.05).

Temperature was correlated with phytoplankton abundance (r = 0.491, p<0.05) and comparably with diatoms (r = 0.447, p<0.05), Cyanophyceae (r = 0.598, p<0.001), Chlorophyceae (r = 0.450, p<0.05) and Euglenophyceae (r = 0.763, p<0.001), yet no significant correlations were found between the biological variables and water salinity. Positive correlations were found between pH and phytoplankton abundance (r = 0.500, p<0.05) and similarly with diatoms (r = 0.458, p<0.05), Cyanophyceae (r = 0.712, p<0.001), Chlorophyceae (r = 0.429, p<0.05) and Euglenophyceae (r = 0.671, p<0.001). Negative correlations were found between phytoplankton abundance and nitrate (r = - 0.783, p<0.001), nitrite (r = -0.701, p<0.001) and ammonia (r = - 0.391, p<0.05). Phytoplankton biomass (Chlorophyll-a) was strongly correlated with phosphate (r = 0.777, p<0.001) and silicate (r = 0.519, p<0.05) and negatively correlated with ammonia (r = - 0.412, p<0.05). Nitrate and nitrite were correlated with diatoms (r = - 0.782 and r = - 0.701 at p<0.001, respectively), Cyanophyceae (r = - 0.656 and r = - 0.568 at p<0.001, respectively), Chlorophyceae (r = - 0.538 and r = - 0.400 at p<0.05, respectively) and Euglenophyceae (r = - 0.635, p<0.001) and (r = - 0.487, p<0.05), respectively. None of the other correlations between dinophyceae and environmental variables were statistically significant (p> 0.05).

Among the predominant phytoplankton species, Skeletonema costatum and Pseudo-nitzschia seriata demonstrated significant positive correlations with temperature (r = 0.604; r = 0.771, respectively) and pH values (r = 0.667, r = 0.793, respectively) at p<0.001.

DISCUSSION

El Dekhaila Harbor is characterized by high nitrogen and phosphorus concentrations8,29,12. The results of chemical parameters after Shaltout and Abd-El-Khalek13, demonstrated that the annual averages nitrate and ammonia concentrations were 6.67 and 13.22 μM, respectively, these values are higher than the levels of eutrophication (4 and 2 μM) proposed by Franco30. Reactive phosphate averaged 5.93 μM, this value is pronouncedly more noteworthy than the eutrophication limit (0.3 μM) that appointed by Marchetti31 and Stirn32. The concentration of reactive silicate increased from 14 μM12 during 1998-99 to achieve 34.72 μM13. Then again, Chlorophyll-a demonstrated a pronounced diminish from an average of 107.5 μg L–112 to a mean of 60.83 μg L–113.

The phytoplankton community in El Dekhaila Harbor was mainly made out of diatoms throughout the year. The pioneer of diatoms could be due to their ability to adjust quickly to the changing in the environmental conditions33. The prevalence of diatoms over different groups is similar to observations in many other eutrophic environments in Egypt, for example, Eastern Harbor and Kayet Bey34-36 and in Rosetta and Abu-Qir37.

Bacillariophyceae consisted of centric (34 species) and pennate (38 species) forms. The dominance of pennate over the centric seems, by all accounts, to be a typical component in various estuaries38. Pennate forms of diatoms observed in this work were mostly represented by Navicula and Nitzschia. The centric forms of Bacillariophyta in El Dekhaila Harbor were generally represented by Skeletonema costatum and too much lesser degree Leptocylindrus danicus. The last species has not been archived before from El Dekhaila Harbor15,11.

Species of dinoflagellates were often recognized, specifically; Prorocentrum micans, Prorocentrum triestenium, Prorocentrum cordata, Prorocentrum minimum and Protoperidenium minutum. These species are known globally as harmful algal bloom-forming genera and are known to produce toxins39.

Phytoplankton community structure in El Dekhaila Harbor was seen to be controlled by 7 diatoms which constituted collectively >60% of the total phytoplankton density; Skeletonema costatum (17.82%), Pseudo-nitzschia seriata (13.68%), Chaetoceros curvisetus (6.10%), Asterionellopsis glacialis (5.95%), Leptocylindrus danicus (5.64%), Thalassiosira rotula (5.55%) and Cyclotella meneghiniana (5.38%). These species as was showed up in eutrophicated environments, for example, in Eastern Mediterranean40,41. Of these, the two first species are neritic, cosmopolitan and consider as an indicator of eutrophication42 and known as toxin production43.

Positive correlation was seen between phytoplankton density and both of water temperature (r = 0.497, p<0.05) and pH values (r = 0.506, p<0.05). Furthermore, cell densities of Bacillariophyceae, Chlorophyceae, Euglenophyceae and Cyanophyceae were correlated with pH and water temperature. Ismael and Dorgham11 demonstrated that salinity is the most important factor controlling the phytoplankton succession in El Dekhaila Harbor.

A negative correlation was seen amongst diatoms and each of NO3 (r = -0.783, p<0.05), NO2 (r = -0.701, p<0.001) and ammonia (r = -0.391, p<0.05), which means that diatoms can assimilate nitrogen in the form of nitrate, nitrite and to less extent ammonium.

Among the nutrients, silicate did not impact on phytoplankton multiplication as it exhibited no significant relation between silicate and phytoplankton abundance (r = 0.007, p = 0.969) and Chl-a (r = 0.519, p<0.001). This correlation implies an excess of silicate present in the system. A comparative observation was clear with PO4, in which it exhibited no correlation with phytoplankton abundance (r = 0.129, p = 0.452), but showed strong correlation with Chl-a (r = 0.777, p<0.001). This indicated that PO4 concentrations play a critical part in governing the instantaneous rates of chlorophyll a and not of phytoplankton density.

Phytoplankton abundance weakly correlated with chlorophyll a (r = 0.452, p<0.05), this might be because of the presence of large numbers of picoplankton or nanoplankton cells which unfortunately were not checked during the present study. The essential portion of the phytoplankton was connected to the presence of diatoms, which were positively correlated with chlorophyll a (r = 0.491, p<0.05) and with dinoflagellates was not significant (r = 0.059, p = 0.733) or other taxonomic groups, so chlorophyll a was just controlled by diatoms.

Chiaudiani and Vighi44 showed when N:P lies in the 4.5-6 range, the assimilation of the two elements by marine algae is about ideal, however at a ratio of >6, phosphorus transforms into the limiting element and when N:P <4.5, nitrogen is the limiting variable. El Dekhaila Harbor was characterized by low N:P ratio during winter and spring and high ratio in summer and autumn. The average values of N:P in winter, spring, summer and autumn were 1.94, 1.08, 44.60 and 208.94, respectively. Diatoms can easily become dominant when developing in non-limiting conditions5, though when nutrients are limited, diatoms are more easily influenced than dinoflagellates, particularly under phosphorus limitation45. However, there is no evidence of nitrogen or phosphorus limitation in El Dekhaila Harbor; indeed, phytoplankton density reflected high phytoplankton abundance all the year round. Moreover, the minimal phytoplankton density in autumn (average 4841±1002 cells mL–1) coincided with the maximum N:P ratios (22-364). It subsequently appears that a variable N:P ratio is not a sign of nitrogen or phosphorus limitation or the species composition of the phytoplankton community. Extreme fluctuation of N:P is common along the Mediterranean coast of Egypt, especially in zones presented to land-based runoff46,47,15,10.

Egge45 considered that when silicate concentration in the system was >2 μmol L–1, diatoms could overwhelm the phytoplankton community if other different nutrients were sufficient. In the 4 cruises, the average silicate concentrations were clearly higher than this value and fluctuating between 10 and 78 μM, this implies that silicate was sufficient and couldn't be limiting factor in El Dekhaila Harbor, thus phytoplankton abundance versus Si:P ratios demonstrated no prominent relationship (r = -0.320, p = 0.057). Roberts et al.48 suggested that diatom development can be restricted by dissolved silica when the N:Si ratio is over 1:1, while Guo et al.49 recommended that N:P ratio, however evidently not Si:N, is argued to be a significant factor in controlling phytoplankton community structure. The mean N:Si ratios were 0.64 in winter; 0.29 in spring; 0.65 in summer, however, the ratio increased to achieve 1.76 in autumn. The ratios in the first three seasons promoted diatom development (6×106-12×106 cells L–1) but during autumn, the DIN:DSi ratios were over 1 (1.76) which makes this period less favorable for diatom development, thus lowest diatom density was recorded during autumn (4×106 cells L–1).

Other than the factors that controlling phytoplankton abundance discussed above, grazing can be of great importance in species succession if there is specific grazing on one particular phytoplankton group. To discuss this relation in El Dekhaila Harbor, multiple regressions between the total zooplankton groups, that studied by Heneash17 and the predominant phytoplankton species and furthermore between selected zooplankton species and the dominant species of phytoplankton were performed using SPSS Programme.

Copepods as the predominant group in El Dekhaila Harbor were significantly and negatively correlated with diatoms as a phytoplankton group (r = -0.502, p<0.05) and with the prevailing species; Skeletonema costatum (r = -0.583, p<0.001); Pseudo-nitzschia seriata (r = -0.509, p<0.05); Thalassiosira rotula (r = -0.466, p<0.05). Additionally, protozoa as a group was negatively correlated with diatoms (r = -0.437, p<0.05) and some of the predominant species; Skeletonema costatum (r = -0.423, p<0.05), Asterionellopsis glacialis (r = -0.464, p<0.05), yet no relation with Leptocylindrus danicus and Thalassiosira rotula. It shows up from the correlations that dinoflagellate as a group or dinoflagellate as a species are unsuitable as a prey for zooplankton; a similar observation was recorded by Ismael and Abdel-Aziz16, thus no relationship was found amongst dinoflagellates and zooplankton or any group and zooplankton species. The results showed a significant negative correlation between copepod Oithona nana and diatoms as a group (r = -0.576 , p<0.001) and with the diatom species as Skeletonema costatum (r = -0.600, p<0.001) and other diatom species at p<0.05 as Pseudo-nitzschia seriata (r = -0.564); Asterionellopsis glacialis (r = -0.488); Leptocylindrus danicus (r = -0.416); Thalassiosira rotula (r = -0.432). Guergues50, examined the gut content of Acanthocyclops americanus and found that it contain mainly the chlorophyte species and concluded also that feeding in A. americanus is not aimless but rather selective.

In spite of the fact that tintinnids represent the second important component among zooplankton, they showed a negative correlation with diatoms (r = -0.487) and other diatom species as Asterionellopsis glacialis (r = -0.501); Skeletonema costatum (r = -0.484) at p<0.05 and stay away from other diatoms species. Smetacek51 presumed that in the pelagic environment the ciliate diet is restricted to nanoplankton and bacterioplankton.

This study confirms the importance of studying the relationship between phytoplankton and zooplankton in any aquatic system, rather than studying the physical and chemical parameters.

CONCLUSION

The results demonstrated that, in El Dekhaila Harbor, the grazing by zooplankton play a leading role in controlling phytoplankton community structure. Copepods as the predominant group prefer diatoms, but dinoflagellates are unsuitable as a prey. The nutrient salts concentrations have a weak impact on phytoplankton and so, the latter being limited rather by zooplankton grazing than by nutrient availability.

SIGNIFICANCE STATEMENTS

This study discovers the possible effect of zooplankton grazing activity on phytoplankton composition and distribution in a harbor suffered with high nutrient salts concentrations. Other combinations that may be also effect on phytoplankton density as water temperature and pH values, yet no significant correlations were found between phytoplankton variables and the concentrations of nutrient salts or their ratios. This study will help the researcher to discuss the role of zooplankton grazing activity on the phytoplankton abundance and community structure in any aquatic system as well as the other abiotic factors.

ACKNOWLEDGMENTS

The authors are thankful to National Institute of Oceanography and Fisheries, Marine Environment Division, Egypt for providing necessary facilities.

REFERENCES
Abdalla, R.R., F.A. Zaghloul, Y.A. Hassan and H.M. Moustafa, 1995. Some water quality characteristics of El-Dekhila Harbour of Alexandria, Egypt. Bull. Nat. Inst. Oceanogr. Fish., 21: 85-102.

Abdel-Aziz, N.E., 2001. Zooplankton in beach waters of the Southeastern Abu Qir Bay. J. Egypt. Acad. Soc. Environ. Dev., 2: 31-53.

Abdel-Halim, A.M. and H.M. Khairy, 2007. Potential impact of some abiotic parameters on a phytoplankton community in a confined bay of the Eastern Mediterranean sea: Eastern harbour of Alexandria, Egypt. Mediterr. Mar. Sci., 8: 49-64.
CrossRef  |  Direct Link  |  

Al-Kandari, M., F. Al-Yamani and K. Al-Rifaie, 2009. Marine Phytoplankton Atlas of Kuwait's Waters. Kuwait Institute for Scientific Research, Kuwait, ISBN-13: 9789990641240, Pages: 354.

Bates, S.S., M.F. Hiltz and C. Leger, 1999. Domoic acid toxicity of large new cells of Pseudo-nitzschia multiseries resulting from sexual reproduction. Proceedings of the 6th Canadian Workshop on Harmful Marine Algae, February 1999, St. Andrews, NB., Canada, pp: 21-26.

Chiaudiani, G. and M. Vighi, 1978. Metodologia standard di saggio algale per lo studio della contaminazione delle acque marine. Quaderni IRSA No. 39, Consiglio Nazionale delle Ricerche, Italy.

Cupp, E.E., 1943. Marine plankton diatoms of the West coast of North America. Bull. Scripps Inst. Oceanogr., 5: 1-238.

Dodge, J.D., 1982. Marine Dinoflagellates of the British Isles. Her Majesty's Stationery Office, London, UK., ISBN-13: 9780112411963, Pages: 303.

Dorgham, M.M., M.I. El-Samra and T.H. Moustafa, 1987. Phytoplankton in an area of multi-polluting factors West of Alexandria, Egypt. Qatar Univ. Sci. Bull., 7: 393-419.
Direct Link  |  

Egge, J.K., 1998. Are diatoms poor competitors at low phosphate concentrations? J. Mar. Syst., 16: 191-198.
CrossRef  |  Direct Link  |  

Fahmy, M.A., F.T. Tayel and M.M. Sheriadah, 1997. Spatial and seasonal variations of dissolved trace metals in two contaminated basins of the coastal Mediterranean Sea, Alexandria, Egypt. Bull. Fac. Sci. Alexandria Univ., 37: 187-198.

Fahmy, M.A., N.E. Abdel-Aziz and M.M. Dorgham, 2004. Water quality observations in the Dekhaila Harbour, Alexandria, Egypt. Arab Gulf J. Scient. Res., 22: 206-216.
Direct Link  |  

Fernandes, L.F. and F.P. Brandini, 2004. Diatom associations in shelf waters off Parana State, Southern Brazil: Annual variation in relation to environmental factors. Braz. J. Oceanogr., 52: 19-34.
Direct Link  |  

Franco, P., 1983. Fattori influent sulla productivita primaria della adriatico settentrionale. Proceedings of the International Conference on Problems of the Adriatic Sea, September 26-27, 1983, Trieste, Italy -.

Gharib, S.M., 1998. Phytoplankton community structure in Mex Bay, Alexandria, Egypt. Egypt. J. Aquat. Biol. Fish., 2: 81-104.

Gowda, G., T.R.C. Gupta, K.M. Rajesh, H. Gowda, C. Lingadhal and A.M. Ramesh, 2001. Seasonal distribution of phytoplankton in Nethravathi estuary, Mangalore. J. Mar. Biol. Ass. India, 43: 31-40.

Guergues, S.K., 1979. Ecological study of zooplankton and distribution of macro-fauna in Lake Manzalah. Ph.D. Thesis, Faculty of Science, Alexandria University, Egypt.

Guo, S., Y. Feng, L. Wang, M. Dai, Z. Liu, Y. Bai and J. Sun, 2014. Seasonal variation in the phytoplankton community of a continental-shelf sea: The East China Sea. Mar. Ecol. Progr. Ser., 516: 103-126.
CrossRef  |  Direct Link  |  

Halim, Y., S.A. Morcos, S. Rizkalla and M.K. El-Sayed, 1995. The impact of the Nile and the Suez Canal on the living marine resources of the Egyptian Mediterranean waters (1958-1986). In: Effects of Riverine Inputs on Coastal Ecosystems and Fisheries Resources, FAO Fisheries Technical Paper No. 349, Rome, Italy, pp: 19-57.

Hallegraeff, G.M., M.A. McCausland and R.K. Brown, 1995. Early warning of toxic dinoflagellate blooms of Gymnodinium catenatum in Southern Tasmanian waters. J. Plankton Res., 17: 1163-1176.
CrossRef  |  Direct Link  |  

Hendey, N.I., 1964. An introductory account of the smaller algae of British coastal waters. Part V: Bacillariophyceae (diatoms). Minist. Agric. Fish. Food, Fish Invest., London, UK., pp: 1-317.

Heneash, A.M.M., 2015. Zooplankton composition and distribution in a stressed environment (El Dekhaila Harbour), South-Eastern Mediterranean Sea, Egypt. Int. J. Adv. Res. Biol. Sci., 2: 39-51.
Direct Link  |  

Ismael, A.A. and M.M. Dorgham, 2003. Ecological indices as a tool for assessing pollution in El-Dekhaila Harbour (Alexandria, Egypt). Oceanologia, 45: 121-131.
Direct Link  |  

Ismael, A.A. and N.E. Abdel-Aziz, 2003. Selective grazing on phytoplankton in El-Dekhaila Harbour (Alexandria). Mar. Life, 13: 21-30.
Direct Link  |  

Khairy, H.M., N.R. Hussein, H.M. Faragallah and M.M. Dorgham, 2014. The phytoplankton communities in two eutrophic areas of the Egyptian mediterranean coast. Rev. Biol. Marinay Oceanografia Agosto, 49: 267-277.

Labib, W., 2002. Phytoplankton variability in the Eastern Harbour (Alexandria, Egypt). Egypt. J. Aquat. Biol. Fish., 6: 75-102.

Lewitus, A.J., E.T. Koepfler and J.T. Morris, 1998. Seasonal variation in the regulation of phytoplankton by nitrogen and grazing in a salt-smarsh estuary. Limnol. Oceanogr., 43: 636-646.
CrossRef  |  Direct Link  |  

Mafra, Jr. L.L., L.F. Fernandes and L.A.O. Proenca, 2006. Harmful algae and toxis in Paranagua Bay, Brazil: Bases for monitoring. Braz. J. Oceanogr., 54: 107-121.
CrossRef  |  Direct Link  |  

Marchetti, R., 1984. Quadro analitico complessivo del risultati delle indagini condotte negli anni 1977-1980. II. Problema dell'eutrofizzazione delle acque costiere dell'Emilia Romagna: Situazione ipotesi di intervento, Regione Emilia Romagna, Bologna. Assessorato Ambiente e Difesa del Suolo-Servizio Tutela e Risanamento Ambientale, Emilia-Romagna, Italy, pp: 1-308.

Margalef, R., 1978. Life-forms of phytoplankton as survival alternatives in an unstable environment. Oceanologica Acta, 1: 493-509.
Direct Link  |  

Nessim, R.B. and A.B. Tadros, 1986. Distribution of nutrient salts in the water and porewater of the Western Harbour of Alexandria, Egypt. Bull. Natl. Inst. Oceanogr. Fish., 12: 165-174.

Nessim, R.B. and F.A. Zaghloul, 1991. Nutrients and chlorophyll in the polluted Kait Bey region, Alexandria. Proceedings of the International Symposium on Marine Chemistry in the Arab Region, April 1991, Suez, Egypt, pp: 71-80.

Opute, F.I., 2000. Contribution to the knowledge of algae of Nigeria. I. Desmids from the Warri/Forcados estuaries. Part II. The elongate baculiform desmids. J. Limnol., 59: 131-155.

Opute, F.I., 2003. Contribution to the knowledge of algae of Nigeria Chlorophyceae from the Warri/Forcados estuaries. Part 1. The orders Volvocales and Chloroccocales. Benin Sci. Dig., 1: 31-52.

Pednekar, S.M., V. Kerkar and S.G.P. Matondkar, 2014. Spatiotemporal distribution in phytoplankton community with distinct salinity regimes along the Mandovi Estuary, Goa, India. Turk. J. Bot., 38: 800-818.
CrossRef  |  Direct Link  |  

Pielou, E.C., 1975. Ecological Diversity. John Wiley Sons, New York, USA., ISBN-13: 9780471689256, Pages: 165.

Prescott, G.W., 1975. Algae of the Western Great Lakes Area. Michigan State University Press, Michigan, USA., ISBN-13: 9780697045522, Pages: 998.

Rabouille, C., F.T. Mackenzie and L.M. Ver, 2001. Influence of the human perturbation on carbon, nitrogen and oxygen biogeochemical cycles in the global coastal ocean. Geochimica Cosmochimica Acta, 65: 3615-3641.
CrossRef  |  Direct Link  |  

Riegman, R., I.A. Flameling and A.A.M. Noordeloos, 1998. Size-fractionated uptake of ammonium, nitrate and urea and phytoplankton growth in the North Sea during spring 1994. Mar. Ecol. Progr. Ser., 173: 85-94.
CrossRef  |  Direct Link  |  

Roberts, E.C., K. Davidson and L.C. Gilpin, 2003. Response of temperate microplankton communities to N:Si ratio perturbation. J. Plankton Res., 25: 1485-1495.
CrossRef  |  Direct Link  |  

Shaltout, N.A. and D.E. Abd-El-Khalek, 2014. Assessment of seawater quality of El-Dekhaila Harbor, Alexandria, Egypt. Asian J. Adv. Basic Sci., 3: 206-216.
Direct Link  |  

Shams El Din, N.G., E.M. Abo El Khair and M.M. Dorgham, 2014. Phytoplankton community in the Egyptian Mediterranean coastal waters. Indian J. Geo-Mar. Sci., 43: 1-9.

Shannon, C.E. and W. Wiener, 1963. The Mathematical Theory of Communications. University of Illinois Press, Urbana, ISBN 0-252-72548-4, Pages: 117.

Smayda, T.J., 1965. A quantitative analysis of the phytoplankton of the Gulf of Panama. II-on the relationship between C14 assimilation and the diatom standing crop. Inter-Am. Trop. Tuna Commn. Bull., 9: 467-531.
Direct Link  |  

Smayda, T.J., 1997. Harmful blooms: Their ecophysiology and general relevance to phytoplankton blooms in the sea. Limnol. Oceanogr., 42: 1137-1153.
Direct Link  |  

Smetacek, V., 1981. The annual cycle of Protozooplankton in the Kiel bight. Mur. Biol., 63: 1-11.
CrossRef  |  Direct Link  |  

Stirn, J., 1988. Eutrophication in the Mediterranean Sea. Proceeding of the Scientific Workshop on Eutrophication in the Mediterranean Sea: Receiving Capacity and Monitoring of Long-Term Effects, March 2-8, 1987, Bologna, Italy, pp: 161-187.

Tayel, F.T.R., M.A. Fahmy and M.M.A. Sheriadah, 1996. Studies on the physico-chemical characteristics of Mex Bay and New Dekhaila Harbour waters of Alexandria, Egypt. Bull. Natl. Inst. Oceanogr. Fish., 22: 1-19.

Turkoglu, M., 2005. Succession of picoplankton (Coccoid Cyanobacteria) in the Southern black sea (Sinop Bay, Turkey). Pak. J. Biol. Sci., 8: 1318-1326.
CrossRef  |  Direct Link  |  

Utermohl, H., 1958. Zur vervollkommnung der quantitativen phytoplankton-methodik. [On improvements in quantitative phytoplankton methods]. Mitteilungen: Internationale Vereinigung Theoretische Angewandte Limnologie, 9: 1-38.
Direct Link  |  

Zaghloul, F., R.. Abdalla, H.M. Mustafa and A. Badr, 1995. Phytoplankton community structure in El-Dekhaila harbor of Alexandria, Egypt. Bull. Nat. Inst. Oceangr. Fish, 21: 103-123.

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