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Ethanolic Leaf Extract of Ocimum gratissimum Abrogates Methotrexate-induced Liver Injury in Albino Rats



Elias Adikwu, Bonsome Bokolo and Othuke Bensandy Odeghe
 
ABSTRACT

Background and Objective: Limited treatment options made hepatotoxic effect of methotrexate (MTX) a serious health challenge. In folklore, Ocimum gratissimum is used for the treatment of liver disease. The current study assessed the hepatoprotective effect of the ethanolic leaf extract of Ocimum gratissimum (EEOG) against MTX-intoxicated albino rats. Materials and Methods: Forty eight healthy adult albino rats were randomized into 8 groups of 6 rats per group. Rats were pretreated orally with 50, 100 and 200 mg kg–1 of EEOG daily for 5 days and 20 mg kg–1 of MTX intraperitoneal on the 5th day. On the 6th day, rats were weighed, sacrificed and blood was collected. Serum was extracted and evaluated for liver function indices. Liver was excised and assessed for biochemical parameters and pathology. Results: The MTX-treated rats showed significant (p<0.001) alterations in superoxide dismutase, glutathione, catalase, malondialdehyde, glutathione peroxidase, aminotransferase, alanine aminotransferase, alkaline phosphatase, total bilirubin, conjugated bilirubin, lactate dehydrogenase and glutamyl transferase levels when compared to control. The liver of MTX-treated rat showed hepatocyte necrosis. However, the above alterations were mitigated significantly (p<0.001) and in a dose dependent manner in EEOG pretreated rats. Conclusion: Methotrexate-induced hepatotoxicity in albino rats was abrogated by the ethanolic leaf extract of Ocimum gratissimum.

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Received: December 12, 2018; Accepted: March 28, 2019; Published: August 10, 2019

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