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Review Article
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Bio-Valorization Potential of Banana Peels (Musa sapientum): An Overview |
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Parveen Jamal,
Olorunnisola K. Saheed
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Zahangir Alam
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ABSTRACT
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Recent breakthrough in industrial biotechnology offer important economic opportunities for the utilization of agro-industrial residues such as Banana Peels (BP). BP as a complex biological material is an important by-product of several cottage and major hospitality industries. Chemically, it contains cellulose, hemicelluloses, lignin and simple sugars. Due to its availability and value, it is capable as an ideal substrate for microbial process for production of valued-added products. Several efforts have been made to produce protein enriched animal ration, industrial enzymes, citric acid and other industrially viable products. Pre-treatment operations have resulted in improved substrate utilization by microorganisms. Solid State Fermentation (SSF) and submerged state fermentation (SmF) are two promising techniques majorly employed for bioconversion of BP for its value addition. The article reviews recent developments in process and products developed for valorizing BP through biotechnological approach. Information on most recent applications and developments are carefully documented.
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Received: November 23, 2011;
Accepted: January 30, 2012;
Published: February 23, 2012
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INTRODUCTION
The current world economy is no doubt faced with unprecedented decline and
uncertainties and this is evident in how major economies of the world have followed
the downward trend in recent times. One of the major options capable of rescuing
the scenario is agriculture. Agro wastes constitute major byproducts of various
agricultural activities such as cultivation, harvesting and processing. Agro-wastes,
including Banana Peel (BP) have been on the increase due to improved cultivation
techniques and sophisticated processing conditions by allied industries that
generates thousands of tones of this solid agro-waste which is generally discarded
(Umoh, 1998; Milala et al.,
2009; Amarnath and Balakrishnan, 2007a). Currently,
BP constitute about 20-40% of waste generated in high banana planting regions
of Africa, the Americas, India and Southeast Asia (Umoh,
1998; Thomas et al., 2002). This high percentage
creates highly challenging situation in effective management of this solid waste.
Chemically, BP contained appreciable level of lingo-cellulosic materials and
other components such as carbohydrates, vitamins, bioactive compounds and minerals
(essential and non essential) thereby qualifying it for various bioconversion
processes (Johann et al., 2007; Dzomeku
et al., 2007). This highly sophisticated technological approach is
a viable option in effective management of BP waste since many countries are
opposed to landfill and other environmentally implicated solid waste management
approaches (Ravi et al., 2011). In recent times,
BP has been utilized for various industrial applications including bio-fuel
production, bio-sorbents, pulp and paper, cosmetics, energy related activities,
organic fertilizer, environmental clean up and biotechnology related processes
(Morton, 1987; Gunaseelan, 2004;
Bori et al., 2007). In the light of numerous
applications of this feedstock, this review will focus mainly on the biotechnological
applications of BP where it functions as the major complex carbon source. Biological
products emanating from BP include biogas, protein enriched animal feed (Rahman
and Huque, 2002), enzymes, bio-sorbents, bio-ethanol, citric acid and bioactive
compounds (Karthikeyan and Sivakumar, 2010; Aarti
and Anita, 2010; Ashraf et al., 2010; Ponnuswamy
et al., 2011).
One of the recent leaps in biotechnological application of BP is the production
of protein enriched animal feed. This was due mainly to increased generation
of the waste caused by improved cultivation practices, yield as well as the
recent drive for conversion of waste to wealth. However, full utilization of
the waste has been hampered due to recovery challenges facing submerged state
fermentation (SmF) and scale up problem of Solid State Bioconversion (SSB).
Nevertheless, the recent production of valuable enzymes from the waste may assist
in offsetting the costs associated with these bioconversion techniques thereby
making the whole process sustainable economically in future (Arotupin,
2007; Vijayaraghavan et al., 2011; Somda
et al., 2011b). Similarly, enzymatic saccharification of cellulose,
though uneconomical are increasingly being adopted for extraction of fruit juices,
starch and oil from agro sources. The ease of enzyme recovery from bio-converted
solid residues makes the system appropriate for nitrogen enrichment.
Several bioactive compounds and anti-nutritional factors which are present in BP have made its sole use for bioconversion very challenging. Many of these active compounds were capable of being used for treatment of various health issues effectively thereby making the supply of BP more stringent and challenging when needed for biotechnology related activities. However, this challenge seems to be on low side due to improved cultivation techniques.
As mentioned earlier, the scope of this paper will center on the biotechnology
utilization of BP which covers its use for bio-ethanol production, animal feed
protein enrichment and other industrially important products. Various lingo-cellulosic
materials such as sugarcane bagasse, paper and pulp, cassava waste, saw dust
etc have been used in recent times to produce bio-fuels (Pandey
et al., 2000; Akinyele et al., 2011;
Amarnath and Balakrishnan, 2007b). To this end Brazil
and the USA are the two major ethanol producers accounting for over 62% of the
world production (Kim and Dale, 2004). In Brazil for
example, commercial scale fuel ethanol is mainly based on sucrose from sugarcane
while in the USA corn starch is the main feedstock adopted for bio-ethanol production
(Nibedita et al., 2011). These methodologies
are undesirable since large amount of human food are used thereby causing staple
food shortages amidst global hunger and famine. This makes Agro-wastes such
as BP the most reliable source of feed stocks.
In Malaysia, the major agro-waste being generated is Palm Oil Mill Effluent
(POME) and such remained the most researched byproducts from industrial activities.
Several value added products are being produced from it but effective management
remained daunting due to efficacy and efficiency of conversion. However, BP
as one of highly cultivated fruit in Malaysia (Ratule et
al., 2007) appears to generate solid wastes which may be managed efficiently
with the potential in producing bio-products by biotechnology approach, hence
offsetting huge costs associated with POME management and its associated environmental
implications. This study therefore aims at analyzing the biotechnological potentials
of BP in bio-ethanol, animal feed protein enrichment, enzyme, bio-methane production
and other industrially important products.
Raw material: Banana is a tropical fruit grown in over 122 countries
worldwide. Until 2004, the cultivated area of 3.8 million hectares and a total
production of 56.4 million metric tones of the fruit were produced ranking it
fourth behind rice, corn and milk (John and Marchal, 1995;
Chai et al., 2004; Arumugam
and Manikandan, 2011). In Malaysia however, banana is the second most widely
cultivated fruit and occupies 27,500 ha out of 250,000 ha dedicated for fruit
cultivation thereby constituting 11.0% of the total fruit area in the country.
A total of 530,000 metric tones of the fruit were produced through small farm
holders and large scale farmers (Chai et al., 2004;
Husain and William, 2010). The top ten world producers
of banana are presented in Table 1. The distributions showed
that Asian countries (India, Philippines, China, Indonesia and Thailand) had
more productions while Latin America countries (Ecuador, Brazil, Columbia, Costa
Rica, Mexico) were seconding. Banana wastes (peel) contained important constituents
(Table 2), these qualifies it for several biotechnological
processes involving the use of chemical and biological systems capable of transforming
lignocellulosic materials to value added products. Carbohydrate composition
of banana peels are presented in Table 3.
Agro-residues are important raw materials used for animal feeding, domestic
heat generation, compost and firing of boilers. Valuable portions of BP contained
promising constituents based on varieties and geographic divide and climatic
conditions in the producing regions of the world (Kim and
Dale, 2004). Annually, large amount of agro related wastes are disposed
of indiscriminately into the water bodies, left to rotten constituting environmental
problems and spread of diseases. Similarly large amount of vegetable wastes
are recorded in developing countries due to poor handling and inefficient transportation.
In India for example, the estimated fruit and vegetable production was 150 million
tones and the total waste generated was 50 million tones which amount to one
third of annual production. Out of this high value, about 6.5 metric tones is
BP (FAO, 2009). Common practices in high banana producing
countries is to leave the peels to decay as compost on farm lands or uncontrolled
dumping in landfills where it rotten out without meaningful reuse (Arumugam
and Manikandan, 2011). Several countries have banned open air dumping of
solid waste and this policy is a common practice in many countries in Europe,
Americas and Asia countries (Nibedita et al., 2011).
Table 1: |
Top ten banana producing nations |
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Source: Food and Agricultural Organization of the United Nations
(FAO, 2009) |
Table 2: |
Minerals and nutritional composition of Musa sapientum
peel |
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Trp: Tryptophan, Val: Valine, Met: Methionine, Ile: Isoleucine,
Leu: Leucine, Thr: Threonine, Cys: Cysteine, Tyr: Tyrosine, Phe: Phenylalanine,
His: Histidine, Lys: Lysine |
Banana peels contains several important micro and macronutrients as presented
in Table 2. The composition of almost all essential amino
acid (leucine, valine phenylalanine and threonine) at levels above Food and
Agriculture Organization (FAO) standard as well as crude fat and polyunsaturated
fatty acids (particularly linoleic and α-linolenic acid) makes it an important
basal material included in animal feed.
Table 3: |
Carbohydrate composition of banana peels |
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Upon ripening, several degradative reactions caused by endogenous enzymes
are believed to affect starch and hemicelluloses composition of the peels and
this explains its elevated sugar content (Thomas et al.,
2007; Debabandya et al., 2010). This chemical
conversion process makes biodegradation of BP easy when needed for other biotechnological
use. Moreover, pectin quality of the peels is promising having contained important
simple sugars (glucose, rhamnose, arabinose and xylose) at appreciable levels
(Thomas et al., 2002).
Lignocelluloses are a renewable organic material contained by all plants (including
BP). They consist of three important components: cellulose, hemicelluloses and
lignin. Certain agro-residues may contain trace amounts of ash, proteins and
pectin (Dashtban et al., 2009). Cellulose on
the other hand is a linear biopolymer of anhydroglucopyranose molecules, connected
by β-1, 4 glycosidic bonds. The coupling of adjacent cellulose chains by
hydrogen bonds, hydrophobic interactions and van Waals forces leads to
a parallel alignment of crystal-line structures known as micro fibril (Zhang
et al., 2006). Moreover, hemicelluloses, are heterogeneous polymers
of five carbon, six carbon and sugar acids. Composition of hemicelluloses varies
in nature depending on plant source (Saha, 2000, 2003).
Lignin is a heterogeneous polymer in lignocellulosic materials and generally
contains three aromatic alcohols (coniferyl alcohol, sinapyl and p-coumaryl).
Lignin acts as a barrier for enzymes actions due to its linkage to both hemicelluloses
and cellulose preventing lignocellulolytic enzymes from interior structure of
lignocelluloses. Hence it is the most difficult component of lignocellulosic
material to degrade (Sanchez, 2009; Himmel
et al., 2007).
Pretreatment: One of the most important activities performed in bioconversion
of lignocellulosic materials is pretreatment. This operation is necessary in
order to break strong C-C bonds that exist between the natural components. Therefore,
fulfilling the purpose of all treatment methods which is to disintegrate complex
biopolymers which will eventually expose them to chemical and biological attacks.
The pretreatment is done to break lignocellulosic matrix which reduce the degree
of crystallinity of the cellulose and increase the fraction of amorphous cellulose
suitable for enzymatic and microbial attack (Sanchez and
Cardona, 2008). Similarly, pretreatment processes assist in positive changes
in the macro, micro and subatomic structures of BP thereby freeing up simple
and compound sugars which are more accessible for effective bioconversion. For
a pretreatment process to be effective there must be: (1) formation of monomeric
sugars directly or subsequently by hydrolysis, (2) avoidance and/or degradation
of fermentable sugars, (3) limits formation of inhibitory products, (4) reduction
in energy demands and (5) economical (Nibedita et al.,
2011). The four fundamental pretreatment methods usually adopted for BP
are: physical, chemical, physicochemical and biological. In other instances
more than one pretreatment technique may be employed depending upon the kind
of end products targeted microorganisms involved.
Chemical pretreatment is the most adopted technique for preparing BP for biotechnological
processes. The sequential use of acid and alkaline (NaOH and HCl) have been
reported to give satisfactory results in producing citric acid, single cell
protein and enzymes (Ponnuswamy et al., 2011;
Karthikeyan and Sivakumar, 2010; Essien
et al., 2005). Essien et al. (2005)
also included heat processing by subjecting the substrate to heating at 121°C
for 10 min claiming the substrate perform better as compared to the agar type
which was heated at same temperature for 15 min. Similarly, chemical pretreatment
(NaOH and HCl) was done using swelling agent by refluxing ground peels at 121°C
for 60 min and substrate washed thoroughly before drying (Yabaya
and Ado, 2008).
Hydrothermal pretreatment was adopted by Oberio et al.
(2011) to produce bio-ethanol from BP. this method, generally entails size
reduction of BP, conditioning followed by continuous mixing with superheated
water (180-200°C) for 15 min in order to destroy protecting lignin structure
and make cellulose available for enzymes and microbial attack. Through this
method, 28.2 g L-1 and 2.3 g L-1 h-1 ethanol
concentration and productivity were recorded respectively as the highest values
reported in recent times. Xue et al. (2011) carried
out dilute acid pretreatment (1% (v/v) dilute sulfuric acid at 120°C for
20 min) on powdered BP to produce glutathione. A high Dry Cell Weight of 7.68
g L-1 and glutathione yield of 111.33 mg L-1 was reported
with the initial sugar concentration of 20 g L-1 in the hydrolyzate
of banana peels. Arumugam and Manikandan (2011) compared
acid treatment with liquid hot water pretreatment. Dilute acid pretreatment
significantly increased the sugar release by nearly 20% over the liquid hot
water. Same trend was reported by Naresh et al. (2005) for release of
fermentable sugars from BP and Kinnow waste. According to Aden
et al. (2002) the main advantage of dilute acid pretreatment related
to other pretreatment methods is the higher recovery of sugars derived from
hemicellulose. The dilute acid pretreatment has the advantage of not only solubilizing
hemicelluloses but also converting solubilized hemicelluloses to fermentable
sugars and thus, eliminates or reduces the need for use of hemicellulase enzymes
mixtures (Nigam, 2002).
Bioprocess techniques: Biotechnological processes involving cultivation of microbes on BP can be generally classified into two namely: Submerge state fermentation (SmF) and Solid State Fermentation (SSF). Substrates (BP) in SmF can be used as sole carbon source, co-substrate, part of mixed fruit peels or as hydrolysates form of BP. Solid state on the other hand can be such that BP is used as complex carbon source or as co-substrates (as inert solid support). Similarly, SSF can involve monoculture or co-culture of more than one microbial strain. The uses of the substrate mostly depend on the type, yield and economies surrounding the whole processes and product.
Application of BP in SmF: Numerous biotechnological products have been
produced from BP and these include cellullolytic and non cellulolytic enzymes,
bio-ethanol, Single Cell Protein (SCP), biogas etc. (Yabaya
and Ado, 2008; Essien et al., 2005; Ravi
et al., 2011; Enwefa, 1991; Nirmala
et al., 1996; Xue et al., 2011). Table
4 shows some examples of bio-products from BP in SmF.
Single Cell Protein (SCP) or bio-protein remained one of the products of interest
made from BP. Several liquid state efficient microorganisms (yeast, bacteria
and filamentous fungi) have been employed. They included lignolytic and non-lignolytic
fungi, highly flocculent, thermo-and osmo-tolerant yeast strains which produce
several hydrolytic enzymes capable of degrading the celluloses, lignin and hemicelluloses
of the BP after pretreatment operations.
Table 4: |
Bio-products produced from BP by SmF |
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Essien et al. (2005) carried out fermentation
on BP by preparing Banana Peel Agar (BPA) comprising Banana peel extract, Agar
powder and Distilled water (200 g, 15 g, 1000 mL) at this order respectively.
The control was Malt Extract Agar (MEA). Biomass growth on both MEA and BPA
was insignificant; however, MEA shows promising potential of BP in supporting
protein enriched biomass for cattle feed protein enrichment. Ravi
et al. (2011) produced bio-protein from BP using Trichoderma harzianum
studying the effect of different carbon and nitrogen sources. Sucrose gave the
best result in biomass yield (961.57 mg) and protein synthesis (0.73 g L-1)
while sodium nitrate was the best nitrogen source with 970.53 mg of biomass
and 0.78 g L-1 protein production. Similarly, the cultivation of
Saccharomyces uvarum on BP at different concentration (1.25-10% w/v)
with ammonium sulphate as nitrogen source produced impressive biomass concentration,
crude protein and cell protein (4.98, 0.89 and 2.90 g L-1), respectively.
High osmotic and sugar effects were opined to affect biomass yield at higher
BP concentration (Enwefa, 1991). It is worthy of mention
that BP is capable of supporting microbial growth with little supplementation.
The hydrolysis of BP chemically breakdown the hemicelluloses yielding monomeric
sugars (xylose, glucose, mannose, arabinose, galactose) and its pentosan component
contains mainly D-xylose and arabinose. BP hydrolysate however, may also contain
toxic substances (depending upon the type of hydrolysis) which may cause lyses
effects on fermentative and non-fermentative micro-organisms. Acidic and alkaline
treatment as well as other pre-treatment techniques could be adopted to overcome
these inhibitory effects (Ogier et al., 1999;
Somda et al., 2011a).
Enzyme production from agro-wastes has been widely researched with promising
results signaling their possibility of taking over from food based (e.g., use
of corn to produce amylase) enzyme production which has been advocated against
in many quarters. Bhat et al. (2010a) conducted
two intensive researches producing α-amylase by using strains of Bacillus
subtilis (MTCC 441). In one of the research, BP was used as sole carbon
source after screening studies was conducted among various carbon and nitrogen
sources by Central Composite Design (CCD). Further optimization study conducted
by Artificial Neural Network (ANN) showed close relationship between predicted
(1398.00 U mL-1) and experimental values (1545.09 U mL-1)
(Bhat et al. (2010a). In the second investigation
on Bacillus subtilis by same author, BP and corn pitch were utilized
as substrates for α-amylase production. Enzyme production was 1799 U mL-1
for BP and 671 U mL-1 for corn pith. Further increase in enzyme activity
was recorded after optimization studies by Taguchi method where BP gave 1580
and 530.32 U mL-1 activity was recorded for corn pith (Bhat
et al., 2010b).
Intensive research on BP hydrolysate is still at infancy but few researches
conducted showed its potential for bio-ethanol and glutathione production.
Arumugam and Manikandan (2011) conducted intensive investigation
on BP hydrolysate for bio-ethanol production adopting dilute acid pretreatment
and saccharification techniques. Dilute sulphuric acid was used for the former
and α-amylase and gluco-amylase enzymes from Bacillus subtilis and
Aspergillus niger were used for the latter. These pre-treatments released
enough sugar for bio-ethanol production yielding 8.66% w/w when liquid hot water
pre-treatment was combined with enzyme saccharification, whereas 13.84% w/w
was recorded for combined dilute acid pre-treatment and enzyme saccharification.
Similarly, Xue et al. (2011) conducted intensive
study on BP for glutathione production by Candida utilis (SZU 01-01).
In his findings, 85.69 mg L-1 of enzyme activity were recorded at
initial stage while upon full media optimization, the production increased to
154.32 mg L-1.
Application of BP in solid state fermentation: Solid State Fermentation
(SSF) is an excellent and efficient biotechnological approach capable of producing
important industrial products from agro-wastes which were previously causing
environmental concerns. In recent times, there has been a resurgent interest
in the adoption of SSF as a more robust approach in producing second generation
bio-products from agricultural and non-agricultural waste products due to its
many advantages over SmF (Ghildyal et al., 1985;
Ashok, 1991). Several SSF investigations have been conducted
regarding BP where it was utilized as main carbon source while in other applications;
it was used as co-substrates to augment fermentation systems for efficient bio-product
synthesis (Johann et al., 2006, 2007;
Aarti and Anita, 2010; Arumugam
and Manikandan, 2011; Ponnuswamy et al., 2011;
Omojasola and Jilani, 2009; Brooks,
2008).
Enzymes production from BP has been of interest to most researchers due mainly
to its monomeric (simple) sugar content which are easily metabolizable by filamentous
fungi, yeast and bacterial. Α-amylase production potential of BP by various
strains of Bacillus subtilis has been of interest to most investigators.
In order to produce α-amylase from BP, Noreen et al.
(2002) used yeast extract, corn steep liquor, Sodium Dodecyl Sulphate (SDS)
and Tween-80 as supplements and enhancers respectively. An insignificant concentration
of 0.1% yeast extract, 1% corn steep liquor, 0.15% SOS and 0.1% Tween-80 at
pH 7 gave maximum enzyme yield of 10.25 U mL-1. Upon characterization
of the enzyme, maximum activity (12.18 U mL-1) was recorded at 60°C
and pH 6 with 3 hours stability under these conditions.
Ponnuswamy et al. (2011) also produced same
enzyme from Penicillium sp. Utilizing BP as the substrate without any
enrichment with mineral salts or other carbon sources throughout the investigation.
His results showed that more enzymes were produced at SSF when compares to SmF
productivity. However, the optimum enzyme activity was at pH 7 and 50°C
being optimum temperature with 13% loss of activity after 30 min. In the case
of Shaista et al. (2003) investigation on α-amylase,
an effect of nitrogen (peptone) supplementation was tested. Results obtained
showed 0.2% nitrogen augmentation for optimum enzyme activity (9.06 IU mL-1
min-1) for 24 h at pH 7 and 35°C as optimum conditions. Johann
et al. (2006) carried out investigations on laccase synthesis using
BP as sole substrate adopting white rot fungus. A maximum activity of 1570 U
L-1 was recorded signaling the potential of the substrate in supporting
extracellular production of the enzyme. To test for the degrading capacity of
the enzyme, in vitro decolouration of two structurally different dyes such as
the anthraquinonic dye Remazol Brilliant Blue R (RBBR) and the triphenylmethane
dye Methyl Green (MG) was carried out. RBBR was decolourised about 57% in 4
h, whereas MG presented a lower decolouration rate of 40.9% in 4 h. The researchers
then opined that RBBR decolouration was considerably higher than that obtainable
from commercial laccase (23.2% in 4 h), however, MG decolouration of 46% in
4 h was not so different from its commercial counterpart. Further more, the
SEM microphotographs of BP with and without fungus showed effective adherence
of the fungi to the solid support. The investigators however concluded that
hydrophobicity and surface charge are most important characteristics that influence
adhesive behavior of filamentous fungi to the solid support.
Citric acid as one of the industrially important product has been produced
on different substrates by different methods with varied in production and product
performance (Sauer et al., 2008). Several agricultural
residues such as grape pomace, apple pomace, banana waste, sugar beet, okara,
jack fruit carpel and kiwi fruit peel have found their utilization via bioconversion
processes (Angumeenal and Venkappayya, 2005; Hang
and Woodams, 1995; Khare et al., 1995; Vandenberghe
et al., 2000; Sassi et al., 1991;
Shojaosadati and Babeipour, 2002).
Based on the success of other workers, Karthikeyan and
Sivakumar (2010) then conducted an intensive research to investigate citric
acid potentials of BP. Table 5 presented Bio-products produced
from BP by SSF. Important parameters that affect citric acid production such
as moisture content, temperature, pH, inoculum level and incubation time were
investigated. Optimum temperature was 28°C while the moisture content was
70%, initial pH 3, 108 spores mL-1 and 72 h incubation
were other optimum conditions suitable for maximum (180 g kg-1) citric
acid production by Aspergillus niger.
Microbial strains mostly cultivated on BP: In recent years, large numbers of micro-organisms which include bacteria, yeasts and fungi have been cultivated on BP. However, none of these strains has enjoyed universal adoption among investigators. Yeast cells are wide adopted for bio-ethanol; filamentous fungi are preferred for protein enriched animal feed while bacteria cells were commonly used for enzyme production. A list of different micro-organisms cultivated on the BP for varying purpose by different researchers is given in Table 6.
Table 5: |
Bio-products produced from BP by SSF |
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Table 6: |
Microbial cells cultivated on BP |
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Other biotechnologically derived products from BP: Nirmala
et al. (1996) carried out an investigation on the potentiality of
using BP as a substrate for biogas production. In the research, narrow-mouth
aspirator bottles were used as digesters capable of maintaining temperature
at 37°C at laboratory level for the whole conversion period. Each digester
composed of 1.6-1.8 L of active cattle dung slurry (10% w/v) which was added
as inoculum (starter cutlture) to each digester. Upon achieving 40% methane,
cattle dung was gradually replaced by chopped BP, BP powder or pineapple waste.
Biogas produced in the digester was measured by downward displacement of saline
water. Results obtained showed a decrease in methane production after 20 days
while the production was stable between 29 and 40 days of bio-methanation. In
comparison, chopped BP produced more biogas among all the treatments but biogas
production from other substrates was more stable over same period of conversion.
CONCLUSION Conclusively, the utilization of BP in biotechnological process has cut across wide range of products which have potential for industrial application and commercialization. However, the economic viability depends solely on efficient use of the agro-waste. The use of raw BP may have been successful as animal feed but this has not been achieved yet with bio-conversion with improved protein content. The seasonal harvesting of banana plant remained an impediment to constant availability of the feedstock for bio-ethanol production thereby damping its viability for industrial production of the renewable bio-fuel. In addition, the present environmental legislation in most developing countries is not efficient for effective collection of BP residue from major producers. If such situation prevails for long it may remain a lifetime challenge for commercialization of several bio-products from the waste. Current bio-processing techniques look incapable to produce commercial values of secondary products from BP due to environmental implications of effluents that may be generated due to chemical pretreatments of wastes before conversion. Hence, more environmental friendly pre-treatment technologies capable of breaking down all the complex components of BP without production of inhibitory compounds are needed. Several available lignin degrading and edible bacidiomycetes have not been fully explored for their potentials in producing protein enriched animal feed from BP. Therefore, more research is needed in this area to ascertain their potentiality for commercialization. In addition, cultivation of edible mushroom on pretreated BP should be focused to further reduce overdependence on animal protein.
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