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Research Article

Biochemical Composition and Fatty Acid Profile of the Green Alga Ulva reticulata

Annian Shanmugam and Chendur Palpandi

The total lipid, protein, carbohydrate, water and fatty acid contents of seaweed U. reticulata belonging to the class of Chlorophyceae that had been collected from Vellar estuary, southeast coast of India. Water contents of the U. reticulata was found as 75.33%. Carbohydrate, protein and lipid contents have been estimated as 50.248, 8.484, 19.98 and 1.7% of the dry sample correspondingly. The analysis of fatty acids by gas chromatography revealed the presence of myristic acid, palmitic acid, heptadecenoic acid, oleic acid and linoleic acid. Among the fatty acids, palmitic acid was predominant in all the fatty acids studied.

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Annian Shanmugam and Chendur Palpandi, 2008. Biochemical Composition and Fatty Acid Profile of the Green Alga Ulva reticulata. Asian Journal of Biochemistry, 3: 26-31.

DOI: 10.3923/ajb.2008.26.31



Seaweeds have been used since ancient times as food, fodder and fertilizer and as source of medicinal drugs. Today seaweeds are the raw material for industrial production of agar, carrageenan and alginates (Barbara and Cremades, 1993), but their consumption as food in Asian countries are still going on (Mishra et al., 1993). They are nutritionally valuable as fresh or dried vegetables, or as ingredients in a wide variety of prepared foods (Robledo and Pelegrin, 1997). In particular, certain edible seaweeds contain significant quantities of lipids, protein, vitamins and minerals (Norziah and Ching, 2000; Sanchez-Machado et al., 2002; Wong and Cheung, 2000), although nutrient contents vary with species, geographical location, season and temperature (Dawes et al., 1993; Kaehler and Kennish, 1996).

The study of natural products and the chemical constituents occurring in algae is known as phycochemistry. Usually the natural products are the secondary metabolites. The metabolic behaviour of lipids is largely determined by the structure of the component fatty acids. Generally, fatty acids occurring in algal lipids are straight chain, even carbon number molecules and usually contain one or more double bonds. Much work has been done fatty acid composition of microalgae and seaweeds (Marolia et al., 1982; Takagi et al., 1985; Wood, 1988; Liekenjie, 1989). However, information on the fatty acids of seaweeds collected from Parangipettai coast is lacking. The objective of this study was to determine the biochemical composition and fatty acid profile of the green alga U. reticulata originating from Vellar estuary, Parangipettai coast.


Seaweed was collected from Vellar estuary, during low tide. Samples has been cleaned from epiphytes, epifauna, pebbles and other molluscan shells and washed several times in fresh water, blotted and weighed.

The fresh samples were dried at 60°C for 24 h (in hot air oven) and the dried powder was sieved to get fine powder. Total protein was estimated by following the method described by Raymont et al. (1964), total carbohydrate using phenol sulphuric method (Dubois et al., 1956) and total lipid was extracted by Folch et al. (1956) using chloroform: methanol mixture. The moisture content was estimated by subtracting the dry weight from the known wet weight of the sample. Protein, carbohydrate and lipid content of the species of U. reticulata were analyzed and expressed in percentage. An extraction procedure for fatty acid was followed as described by Bligh and Dyer (1959). Identification and quantification of fatty acids were done using Agilent Technologies 6890 N, Network GC system.


Lipids, proteins and carbohydrates are the most important biochemical components in algal biomass. Lipids are rich in -C = O- bonds, providing much more energy in oxidation processes than other biological compounds. Mainly owing to their high reduction levels, they constitute a convenient storage material for living organisms. In algae, the lipids are widely distributed, specially in several resistance stages (Miller, 1962).

Carbohydrate is the most important component for metabolism and it supplies the energy needed for respiration and other metabolic processes. In the present experiment the carbohydrate content of U. reticulata was found as 50.248% (Fig. 1). The findings of the present experiment coincide well with that of carbohydrate contents reported by the earlier studies like Reeta and Kulandaivelu (1999). They found that the carbohydrate contents were 48.4% of Gracilaria spp. in dry weight basis. Hossain et al. (2003) reported that the carbohydrate contents were 19.93 and 20.81% in sample 1 and 2 from Sargassum horneri. Reeta (1993) has studied the carbohydrate content varied from 6.65 in March to 15.18 in January in S. wightii. Haroon (2000) investigated the carbohydrate contents were 54.71±8.17% of DW in Enteromorpha spp. Sasikumar (2000) studied the two species of green algae Enteromorpha intestinalis and C. linum, the carbohydrate contents were 20.4 to 54.6%. The highest average total carbohydrate contents from U. rigida (Chlorophyta) was 63.04±29.15 kg-1 D.W (6.3%) (Dere et al., 2003). Whereas in the present investigation the carbohydrate contents was reported as 50.248% which is higher than that of S. wightii and lower than that of the others such as Enteromorpha spp., C. linum and U. rigida. Lipids and carbohydrate contents should obviously be very variable depending upon the state of nutrition of cells (Ricketts, 1966).

Fig. 1: Biochemical composition of green alga U. reticulata

Protein are having crucial functions in all the biological processes. Their activities can be described by enzymatic catalysis, transport and storage, mechanical sustentation, growth and cellular differentiation control (Stryer, 1988). In the present study the protein content was estimated as 19.98% in U. reticulata (Fig. 1). The findings of the present experiment coincide well with the protein contents reported by Sasikumar (2000) who has reported the varying protein content between 5.2 and 18.6% in Enteromorpha intestinalis and C. linum. Dere et al. (2003) observed the maximum protein content (0.94 to 31.03%) in some of the Rhodophyta and some green seaweeds belonging to the genus Ulva, the content was ranging between 2.9 and 28.1%. Burtin (2003) investigated the higher protein contents in green and red seaweeds (on an average of 10-30% of the dry weight). Hossain et al. (2003) reported the protein content of 22.38 and 21.96% of dry sample 1 and 2 from S. hornei. Roslin (2003) calculated the protein content in Chlorophyceae and found varying varied from 1.5 to 24.8%. In Phaeophyceae, the maximum protein content was shown by S. ilicifolium (28.2%) and S. wightii (28.2%). In the present study the protein contents were higher than that of the earlier studies like Haroon (2000) i.e., the protein content of Enteromorpha spp. showed the minimum of 9.42±4.62% and maximum of 20.60±5.00% on dry weight basis.

In general the total lipid content was always found less than 4% (Herbreteau et al., 1997) in almost all the seaweeds studied so far. Falling in the same line the lipid content in U. reticulata was also reported as 1.7% (Fig. 1). This general trend is further supported by the findings of Reeta (1993) in S. wightii (0.159 to 1.551%), Reeta and Kulandaivelu (1994) in Gracilaria spp. (0.78 to 3.97%) and Sasikumar (2000) in Enteromorpha intestinalis and C. linum (1.2 to 8.6%). Hossain et al. (2003) also reported 1.38 and 1.96% of Glycolipid in samples 1 and 2 of S. horneri.

Water is biologically significant as an essential metabolite i.e., it participates in the chemical reactions of metabolism. In particular it is used as a source of hydrogen ion in photosynthesis and is used in hydrolysis reactions. In the present study the moisture contents were 75.33% in U. reticulata (Fig. 1). This result is in conformity with the observation of Hossain et al. (2003) where the moisture contents were found as 86.94% in sample -1 and 87.0% in sample-2 from S. horneri.

Lipid represent only 1-5% of algal dry matter and show an interesting polyunsaturated fatty acid composition particularly omega 3 and omega 6 acids which play a role in the prevention of cardio-vascular diseases, osteoarthritis and diabetes. The green algae show interesting levels of alpha linolenic acid (ω3 C 18:3) (Burtin, 2003).

Although seaweeds are not a conventional source of energy (their total lipid content is low), their polyunsaturated fatty acids contents are high when compared to terrestrial vegetable (Darcy-Vrillon, 1993). In the present study saturated fatty acids were dominating (70.01%) the other groups of fatty acids among the total fatty acids and the individual contribution of 16:0 and 14:0 was found as 50.76% and 11.77%, respectively (Table 1). These results are in conforming with the previous reports (Venkatesalu et al., 2003a, b, 2004; Vasanthi et al., 2003). The highest relative percentage of palmitic acid (85.35%) was recorded in Gellidium micropterum (Venkatesalu et al., 2004). The single most abundant fatty acid C16:0 (which in Porphyra spp. accounted for 63.19% of all fatty acid) and (Sanchez-Machado et al., 2004). Palmitic acid was reported as a major fatty acid common to Ergrezia menziesii, Chondracanthus canaliculatus and Ulva lobata (Nelson et al., 2002). The highest relative percentage of myristic acid (7.86) was recorded in Acanthophora spicifera and the highest relative percentage of pantedecanoic acid (1.29) was recorded in Padina tetrastromatica (Venkatesalu et al. 2004). In the present study the myristic acid was recorded in 11.77% and pantedecanoic acid was not recorded.

Table 1: Fatty acid profile (% of total fatty acids) from U. reticulate

The monounsaturated fatty acids (MUFA) were the next most common fatty acids (12.07%) with the higher levels of mostly 18:1ω7c (6.77%). The findings of the present experiment coincide well with the earlier study by Dawczynski (2006) the concentrations of oleic acid were at high levels in Porphyra spp. From China and Laminaria spp. and accounted for more than 20% of total FAME but such a high content was only found in single samples. Furthermore, seaweed varieties tested has low levels of palmitoleic acid (16:1n-7) and the concentrations of this FA ranged from 0.1 to 3.6% of total FAME.

The polyunsaturated fatty acids occupying the third position by contributing 7.96% of total fatty acids that are mainly represented by 18:2ω6c (4.54%). In the previous study by Hossian et al. (2003) reported that the saturated fatty acids 16:0 and 22:0 and monounsaturated fatty acid 18:1 were the major fatty acids for simple lipids.

In India, development of novel foods such as functional foods could be a new possibility for the use of this alga U. reticulata, especially for the protein-rich species, in human nutrition. In general, the lipid contents of all edible seaweeds were low and high levels of polyunsaturated fatty acids of the omega-3 and omega-6 families. This investigation of edible macroalga U. reticulata verified the presence of several health-promoting and beneficial nutrients, such as EAAs, important FAs and highly proteins and low levels of lipids. Further experimentation should be conducted with the samples of all seasons through a year for getting biochemical variation in a year.


Authors are thankful to the Director, CAS in Marine Biology and authorities of Annamalai University for providing with necessary facilities. The authors are also thankful to the Ministry of Environment and Forests, New Delhi for the financial assistance.

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