Parasites of Three Indian Minor Carps of Rajshahi, Bangladesh
Gazi Mahabubul Alam,
The present study attempts to describe the parasitic infestations of three Indian minor carps (Labeo bata, Labeo gonius and Cirrhinus reba) collected from different fresh water bodies of Rajshahi district, Bangladesh during March 2007 to February 2008. A total of 480 host fishes were examined of which 370 fishes were infected by 4 protozoan (Trichodina sp., lchthyophthirius sp., Apiosoma sp. and Chilodonella sp.), 2 monogenean (Gyrodactylus sp. and Dactylogyrus sp.), 2 crustacean (Argulus sp. and Larnaea sp.), 1 digenean (Fellodistomum sp.) and 1 nematoda (Camallanus sp.) parasitic species. These parasites were isolated from body slime, gills and intestine of the infected fishes. Among the isolated parasites Fellodistomum sp. was found as the highest and Chilodonella sp. was found as the lowest in number. Our results indicate that infection and infestation rate of parasites varied with fish size and season and found to be high in the post-monsoon and winter periods (November-March), when fish are most susceptible to parasites.
Received: October 30, 2011;
Accepted: January 02, 2012;
Published: February 25, 2012
Parasitic diseases are playing a vital role by causing mortality or retard
growth rate of fish which lead to economic loss in the farm. Sometimes due to
heavy parasite infestation, particularly the ecto-parasites damage the fish
body or make wounds which facilitates portal of entry for secondary infection
and fish become unfit for human consumption (Hoffman, 1967).
However, five factors namely age, diet, abundance of fishes, independent number
of a parasite within the fish and season, directly influence the parasite fauna
of fishes (Kabata, 1985; Dogiel, 1964).
Srivastava (1975) stated that the characteristic of any
water body can influence and determine its parasitic fauna and when environmental
conditions, such as water, food and temperature become favourable for mass reproduction
of parasites, the disease may spread very quickly.
A majority of freshwater fishes carry heavy infection of parasites which cause
deterioration in the food value of fish and may even result in their mortality.
Besides these, there are a number of helminth parasites which are transmitted
to human beings only through fish. The study of helminth parasites, its frequency
and distribution in fishes, is very scant in Bangladesh. These parasites use
the fish for their shelter and food and thus destruct more or less each and
every organ resulting in pathogenic effects (Lilley et
al., 1992). Parasites interfere with the nutrition, metabolism and secretary
function of alimentary canal, damage nervous system and even upset the normal
reproduction of the hosts (Rahman et al., 1998a,
b). The distribution of helminths of the same host and
their incidence and intensity of infestation varies from one place to another.
One should know these variations in infestation and the taxonomic details of
these worms for proper management of the fish crop in ponds. The presence of
certain helminth parasites, especially larval trematode is known to eliminate
or reduce the reproduction in molluscs (Rahman and Jahan,
Crustacean is an important disease producing parasite of freshwater fishes.
Hoffman (1977) while describing the pathogenicity of
Argulus reported that it can kill a larval eel by a single sting which injects
a cytoplasmic toxin into the host. It is also reported that this parasite creates
ulceration and haemorrhagic changes to the host skin providing ready access
to secondary infections by other parasites, bacteria, fungi and viruses.
Recently, Hossain et al. (1994a, b),
Hafizuddin and Shahabuddin (1996), Akhter
et al. (1997), Rahman et al. (1998a,b),
Parween and Rahman (2000), Banu and
Khan (2004), Rahman and Parween (2001), Alam
et al. (2006), Akter et al. (2007),
Bhuiyan et al. (2007), Uddin
et al. (2012) and Afsharnasab et al. (2009)
have made some attempts to explore the fauna of helminth parasites of fresh
and brackish water fishes and shell fishes in Bangladesh and elsewhere. However,
research on parasitic infestation and infection of freshwater minor carp in
Bangladesh is scarce. Therefore, the present study was aimed to survey the parasites
of Indian minor carp, collected from different fresh water bodies of Rajshahi
MATERIALS AND METHODS
Sample collection: Samples were collected four times per month from
different fresh water bodies of Rajshahi district Bangladesh from March 2007
to February, 2008. A total of 480 fish comprising Indian minor carps; Labeo
bata (n = 180), Labeo gonius (n = 120) and Cirrhinus reba (n
= 180) were used in this study. The fishes were measured to the nearest 1 cm
(total length, TL) and weighed to the nearest 0.1 g (weight).
Grouping of host fishes: In the laboratory, the host fishes were divided into three groups corresponded roughly to smaller (<10 cm TL), medium (10-20 cm TL) and larger (>20 cm TL) to observe whether any relationship exits between length of fish and parasitic infestation.
Collection of parasites: External parasites from body surface, fin and gill were removed by scrapping the slime with a sharp scalpel in a drop of water on a clean dry glass slide and spreaded it evenly. A cover slip was placed over the materials. The gill arches were removed and macerated on slides and examined under a compound microscope (BX51 microscope, Olympus, Japan). In case of monogeneans, the gill were removed into petridishes containing water and gently scrapped to dislodge monogeneans. The monogeneans were removed on to clear slides with a fine pipette through a drop of water and covered with cover slip.
For endoparasites, fishes were dissected out ventrally by a sharp scalpel to observe parasites inside buccal cavity, stomach and intestine. The whole gut was removed in a watch glass containing 0.9% physiological saline afterwards cleaned several times with tap water to free from any unwanted materials. Small worms were searched initially with the help of magnifying glass by scrapping out mucus.
Identification and preservation of parasites: Parasites were identified
according to the description of Yamaguti (1958, 1963),
Hafizuddin and Shahabuddin (1996), Agarwal
and Sharma (1988) and Bykhovskaya-Pavlovskaya et al.
(1962). After identification only metazoan parasites were preserved in AFA
(120 mL distilled water + 6 mL Ethyl Alcohol + 30 mL Formalin + 10 mL Glycerin
+ 0.5% neutral or methylene blue/Acetocarmine) solution for detailed study.
Estimation of parasites: Prevalence, abundance and mean density were
estimated through the following formula proposed by Margolis
et al. (1982) as:
Level of infestation: Level of infestation was measured as follows,
low: 1-5 parasites per slide, medium: 6-10 parasites per slide, high: More than
11 parasites per slide.
RESULTS AND DISCUSSION
A total of 8 ectoparasite species i.e., 4 protozoan (Trichodina sp., lchthyophthirius sp., Apiosoma sp. and Chilodonella sp.), 2 monogenean (Gyrodactylus sp., Dactylogyrus sp.) and 2 crustacean (Argulus sp. and Larnaea sp.) and 2 endoparasite species i.e., 1 digenean (Fellodistomum sp.) and 1 nematoda (Camallanus sp.) were identified from fish samples (Table 1).
Host wise prevalence (%), abundance and mean density of parasites: During the investigation leastwise infection, prevalence, abundance and mean density of protozoan and metazoan parasites were observed (Table 2). The seasonal monthly average prevalence of parasite in L. bata was highest in November (100.00%) and lowest in May and July (66.67%), abundance was highest in November (9.47) and lowest in July (4.27) and mean density was highest in May (11.50) and lowest in July (6.40) (Table 2).
|| Parasites in different organs of three Indian minor carp
|| Average prevalence (%), abundance and mean densities of parasites
in three Indian minor carp species
|+ = Low infection, ++ = Medium infection, +++ = High infection
The seasonal monthly average prevalence of parasite in L. gonius was highest in July (100.00%) and the lowest in November, January and February (60.00%), parasites abundance was highest in July (8.00) and lowest in November (3.50) and mean density was highest in March and October (9.50) and lowest in November (5.83) (Table 2).
The seasonal monthly average prevalence in C. reba was highest in May and October (93.33%) and the lowest in July, August, January and February (66.67%), abundance was highest in October (9.93) and lowest in November (4.27) and mean density was highest in September (12.55) and lowest in November (6.40) (Table 2).
Comparative infestations of Prevalence mean density and Abundance of host
fishes in different length groups
Prevalence: In smaller length group, the highest prevalence value
(82.35%) was found in C. reba and lowest prevalence value (72.73%)
was recorded in L. bata. In medium length group, the highest prevalence
value (97.78%) was found in L. bata and lowest prevalence value (61.25%)
was recorded in C. reba. But in larger length group, the highest prevalence
value (87.50%) was found in C. reba and lowest prevalence value (60.00%)
was recorded in L. bata (Table 3). Golder
et al. (1987) reported that fishes of medium length group were more
infested which is similar with the result obtained in the present study. The
establishment of a host parasite system requires considering factors namely
age and seasons as these factors are basically related with the physiological
and ecological conditions of the host and parasites (Kennedy,
Abundance: In smaller length group, the highest abundance (9.47) was
found in L. bata and lowest abundance (7.38) was recorded in C. reba.
In medium length group, the highest abundance (8.48) was found in L. bata
and lowest abundance (6.06) was recorded in L. gonius.
|| Comparative infestations of prevalence mean density and abundance
of three Indian minor carp species
But in larger length group, the highest abundance (8.28) was found in C.
reba and lowest abundance (1.31) was recorded in L. gonius (Table
3). Gussev (1976) concluded that prevalence and
mean density of infestation generally increased with host size up to a point
and then declined. Yeasmin (2004) noted that the intermediate
length groups of Clarias batrachus and Colisa fasciatus were more
infested than smaller and larger length groups.
Mean density: In smaller length group, the highest mean density (13.03) was found in L. bata and lowest mean density (8.96) was recorded in C. reba. In medium length group, the highest mean density (12.69) was found in C. reba and lowest mean density (8.08) was recorded in L. gonius. But in larger length group, the highest mean density (9.46) was found in C. reba and lowest mean density (2.10) was recorded in L. gonius (Table 3).
During the study period, the variation in species composition, prevalence,
abundance, intensity and infection level of the study of host specimens might
be due to host specificity, temperature, metabolic activity and suppression
of natural immune system of fish. The association of parasites fauna in the
host fishes in the present study are supported by the findings of Akhter
et al. (1997), Chandra et al. (1997),
Hossain et al. (2000), Rahman
(2000), Rahman and Parween (2001). So, it was observed
that among the different size groups of fishes, the prevalence was highest in
medium length groups, abundance and mean density was highest in smaller length
groups as reported by Golder et al. (1987).
The collected parasites were Chilodonella sp., Trichodina sp.,
lchthyophthirius sp., Apiosoma sp., Gyrodactylus sp., Dactylogyrus
sp., Argulus sp., Larnaea sp., Fellodistomum sp. and Camallanus
sp. Whitish to yellowish cysts in the skin and gill, loss of mucus, slight haemorrhage
at the base of dorsal, pectoral and caudal fin were observed in case of heavily
infected fish. Subasinghe (1992) studied hatchery diseases
of freshwater fish in Sri Lanka and reported heavy mortality in major carp fry
and fingerlings due to ciliate ectoparasite like Trichodina sp., lchthyophthirius
sp., Chilodonella sp. and fluke like Dactylogyrus sp. during
nursery operation. Moreover, Mohan (1999) reported that
ectoparasites, protozoans, monogenetic trematodes, fish lice, anchor worm, endoparasitic
protozoans are some of the very important pathogens that have had significant
impact on the yield in carp hatcheries and seed production centres in India.
Perhaps parasite acts either as a pathogen or vector for diseases (Roberts
et al., 1986). Although, Reungprach et al.
(1983) found no direct relationship of ectoparasite with the occurrences
of dermal lesion.
This study describes the parasitic infestations of three Indian minor carps from different fresh water bodies of Rajshahi district, Bangladesh and indicates that infection and infestation rate of parasites varied with fish size and seasonality. This could be due to stocking density, water depth, temperature along with other physico-chemical parameters and management practices maintained. Nevertheless, more in depth research is needed to be carried out for studying parasites as well as diseases of this three minor carp species and other carp fishes to depict precise and full information of carp fish diseases in Bangladesh.
Heartiest thanks to our dedicated laboratory technicians, University of Rajshahi Bangladesh for their support during the study. Thanks to University Kebangsaan Malaysia for the financial support through Young Researcher incentive grant No. GGPM-2011-057. We would also like to thank the anonymous reviewers for their very useful comments.
1: Afsharnasab, M., R. Mortezaei, V. Yegane and B. Kazemi, 2009. Gross sign, histopathology and polymerase chain reaction observations of white spot syndrome virus in shrimp specific pathogen free Litopeneaus vannamei in Iran. Asian J. Anim. Vet. Adv., 4: 297-305.
CrossRef | Direct Link |
2: Agarwal, N. and R. Sharma, 1988. A new species of the monogenean genus Heteromazocraes marnaevi, 1981, from a freshwater syprinid Secaricula gora (Ham.) from the Lucknow district, India. Syst. Parassitol., 11: 59-64.
3: Akhter, M., J. D'Silva and A. Khatun, 1997. Helminth parasites of Anabas testudineus (Bloch.) in Bangladesh. Bangladesh J. Zool., 25: 135-138.
4: Akter, M.A., M.D. Hossain and M.R. Rahman, 2007. Parasitic disease of exotic carp in Bangladesh. J. Agric. Rural Dev., 5: 127-134.
CrossRef | Direct Link |
5: Alam, M.M., M.R. Rahman, K.A. Huq and S. Parween, 2006. Intensity of helminth parasitic infestation in three freshwater fish species of Rajshahl. South Asian J. Agric., 1: 1-3.
6: Banu, A.N.H. and M.H. Khan, 2004. Water quality, stocking density and parasites of freshwater fish in four selected areas of Bangladesh. Pak. J. Biol. Sci., 7: 436-440.
CrossRef | Direct Link |
7: Bhuiyan, A.S., S. Akther and G.M. Musa, 2007. Occurance of parasites in Labeo rohita (Hamilton) from Rajshahi. Univ. J. Zool. Rajshahi Univ., 26: 31-34.
8: Bykhovskaya-Pavlovskaya, I.E., A.V. Gussev, M.N. Dublinina, N.A. Izyunova and T.S. Smirnova et al., 1962. Key to Parasite of Freshawater of the USSR. Acad. Sci. USSR. Zool Inst., USSR., pp: 239-445.
9: Chandra, K.J., K.Z. Islam and R. Wooten, 1997. Some aspects of association and development of Lytocestus indicus in catfish Clarias batrachus. Bangladesh J. Fish. Res., 1: 31-38.
10: Dogiel, V.A., 1964. General Parasitology. Oliver and Boyd, Edinburgh, UK., Pages: 516.
11: Golder, M.I., K.J. Chndra and A.K.A. Rahman, 1987. Helminth parasitism in Nandus nandus (Hamilton). Bangladesh J. Fish., 10: 11-12.
12: Gussev, A.V., 1976. New species of monogenidae from fishes of Cylon. Ind. J. Helm., 16: 53-93.
13: Hafizuddin, A.K.M. and M. Shahabuddin, 1996. Parasitic monogeneans from some freshwater fishes of Comilla, Bangladesh. Chittagong Univ. Stud. Sci., 20: 113-126.
14: Hoffman, G.L., 1977. Argulus: A Branchuiran parasite of freshwater fishes. Fish Disease Leaflet, US Fish and Wildlife Service, USA.
15: Hoffman, G.L., 1967. Lesions due to Internal Helminths of Freshwater Fishes. In: The Pathology of Fishes, Ribelin, W.E. and G. Higaki (Eds.). The University of Wisconsin Press, Madison, Wisconsin, USA., pp: 151-186.
16: Hossain, M.A., A.N.H. Banu and M.H. Khan, 1994. Prevalence of ectoparasites in carp nursery operations of greater Mymensingh. Prog. Agric., 5: 39-44.
17: Hossain, M.A., A.N.H. Banu, M.H. Khan and N. Sultana, 1994. Bacterial microflora isolated from carp and catfish fry and their sensitivity to some antibiotics. Bangladesh J. Microbiol., 11: 95-101.
18: Hossain, M.M., K.J. Chandra and S.K. Mohanta, 2000. Monogenetic trematodes from Puntius stigma of Mymensingh, Bangladesh. Rev. Parasitol., 17: 217-224.
19: Kabata, Z., 1985. Parasites and Disease of Fish Cultured in the Tropics. Taylore and Francis Ltd., London, UK., ISBN-13:9780850662856, Pages: 318.
20: Kennedy, C.R., 1975. The natural history of Slapton Bay Natural Reservior.VIII: The parasite of fish with special reference to their use as source of information about the aquatic community. Fiel. Stud., 4: 177-189.
21: Lilley, J.H., M.J. Phillips and K.A. Tonguthai, 1992. A review of epizootic ulcerative syndrome (EUS) in Asia. Aquatic Animal Health Research Institute and Network of Aquatic Centres in Asia-Pacific, Bangkok, Thailand, pp: 73.
22: Margolis, L., G.W. Esch, J.C. Holmes, A.M. Kuris and G.A. Shad, 1982. The use of ecological terms in parasitology (Report of an ad hoc committee of the American Society of Parasitologists). J. Parasitol., 68: 131-133.
CrossRef | Direct Link |
23: Mohan, C.V., 1999. Social and economic impacts of aquatic animal health problems in aquaculture in India. Proceedings of the Aquatic Animal Health Care in Rural Aquaculture, September 27-30, 1999, Dhaka, Bangladesh, pp: 50-.
24: Parween, S. and M.R. Rahman, 2000. Distribution of helminth parasites in different organs and their monthly rate of infection in three freshwater fishes of Rajshahl. Univ. J. Zool. Rajshahi Univ., 19: 67-72.
25: Rahman, M.R., 2000. Freshwater gastropods in relation to helminth parasites of Bangladesh. Ph.D. Thesis, University of Rajshahi, Bangladesh.
26: Rahman, M.R. and M.S. Jahan, 2005. Larval trematode parasites of freshwater gastropods. Bangladesh J. Zool., 33: 13-34.
27: Rahman, M.R. and S. Parween, 2001. Parasitic infestation in relation to length of Heteropneustes fossilis, Channa punctatus and Colisa fasciatus. Univ. J. Zool. Rajshahi Univ., 20: 53-56.
28: Rahman, M.R., M.A. Prvez, M.S. Jahan and M.M. Sarker, 1998. Histopathology of Beffamya bengalensis (Lamarck) by larval helminth. Univ. J. Zool. Rajshahi Univ., 17: 19-27.
29: Rahman, M.R., S, Parween and H. Ara, 1998. A brief report on two helminth endoparasites from Mastacembelus armatus (Lacepede). Univ. J. Zool. Rajshahi Univ., 17: 75-77.
30: Reungprach, H., S. Boonyaratpalin, J. Supamatya, L. Kesomchandra, W. Polsheivin and J. Sadvakdee, 1983. Special report of the fish disease outbreak committee in Thailand. Ministry of Agriculture and Cooperative, Thailand, pp: 64.
31: Roberts, R.J., D.J. Macintosh, K. Tonguthai, S. Boonyaratpalin, N. Tayaputch, M.J. Phillips and S.D. Millar, 1986. Field and laboratory investigation into ulcerative fish diseases in the Asia-Pacific region. Tech. Report of FAO Project TCPIRAS14508. Bangkok, Thailand, Pages: 214.
32: Subasinghe, R.P., 1992. Hatchery Diseases of Freshwater Fish in Sri Lanka. In: Diseases in Asian Aquaculture, Shariff, M., R.P. Subasinghe and J.R. Arthur (Eds.). Asian Fisheries Society, Philippines.
33: Uddin, M.J., N.B. Kamsol, Z. Yasin and A.T. Shau- Hwai, 2012. Parasites of slipper-cupped oyster Crassostrea iredalei from pulau betong, west coast of penang, Malaysia. Asian J. Anim. Vet. Adv., 71: 173-179.
CrossRef | Direct Link |
34: Yamaguti, S., 1958. Systema Helminthum. Volume I. The Digenetic Trematodes of Vertebrates. Part I and II. Interscience Publisher Inc., New York, Pages: 175.
35: Yamaguti, S., 1963. Systema Helminthum Monogenea and Aspidocotylea. Vol. 4, Intersciences Publishers, New York, London, Pages: 699.
36: Yeasmin, M.M., 2004. Studies on disease carp at Rajshahi region. Bangladesh. J. Zool., 26: 135-140.
37: Srivastava, C.B., 1975. Fish pathological studies in India: A brief review. Dr. B.S. Chauhan Commemoration, pp: 649-358.