Pharmacokinetics of Levofloxacin Following Intravenous and Intramuscular Administration in Cattle Calves
Levofloxacin is a third generation fluoroquinolone with excellent tissue penetration and efficacy against respiratory and urogenital bacterial infections. In the present study, disposition kinetics of levofloxacin was studied in cattle calves following an intravenous (i.v.) and intramuscular (i.m.) administration at a dose rate of 10 mg kg-1. Blood was collected at predetermined time intervals and plasma was separated. Plasma concentrations of levofloxacin were determined using the HPLC assay method (R2 = 0.999). Plasma concentration versus time data was subjected to compartmental pharmacokinetic analysis using nonlinear iterative computer software PHARMKIT. Following intravenous and intramuscular administration of levofloxacin in cattle calves (10 mg kg-1), the plasma concentration versus time data of levofloxacin was best described by two-compartment open model and one-compartment open model with first order absorption rate constant with modest bioavailability values, respectively. The mean values for t½α and t½β were 0.05±0.01 h and 2.12±0.21 h, respectively for i.v. route. The values of AUC, Vdarea and ClB were found to be 29.32±1.19 μg mL-1 h, 1.05±0.10 L kg-1 and 0.34±0.01 L h-1 kg-1, respectively while the ratio of drug concentrations between the tissues and plasma (T/P) was 4.47±02.09. For i.m. route, the mean (±SE) values of t1/2Ka, t½β, AUC, MAT, MRT and F were found to be 0.51±0.09 h, 2.76±0.36 h, 18.43±2.15 μg mL-1 h, 1.85±0.46 h, 4.72±0.72 h and 62.65±5.99%, respectively. Based on the pharmacodynamic indices, the optimal dose of levofloxacin in cattle calves may be proposed as 10 mg kg-1 b.wt. repeated at 24 h interval preferably by intravenous route for treating common microbial infections of veterinary importance.
Received: February 23, 2012;
Accepted: May 21, 2012;
Published: July 10, 2012
Levofloxacin is an S-enantiomer (L-isomer) of ofloxacin with double the potency
of ofloxacin (Tanaka et al., 1993). Ofloxacin
and levofloxacin share basic similarity in their core structure but their physicochemical
properties, pharmacokinetic characteristics and microbial activities vary markedly.
Levofloxacin is well distributed to target body tissues and fluids in the respiratory
tract, skin, urine and prostate. In addition, its cellular uptake along with
poor central nervous system penetration makes it more favourable for use against
intracellular pathogens (Langtry and Lamb, 1998). Bacterial
infections are a common occurrence in cattle calves due to contaminated feed
and water (Luga et al., 2007). The clinical situation
is further aggravated due to emergence of resistance against the common antimicrobials
in such situation, Levofloxacin can be a promising therapeutic tool for several
respiratory and urogenital tract infections (Ram et al.,
2008; Kumar et al., 2009) in cattle calves
which comprise an important component of the Indian livestock population.
The pharmacokinetic profile of levofloxacin has already been studied in large
number of animal species including in cross bred calves (Dumka
and Srivastava, 2006), sheep (Patel et al., 2012a),
buffalo calves (Ram et al., 2008, 2010),
goat (Ram et al., 2008; Mishra
and Roy, 2007), cats (Albarellos et al., 2005),
stallions (Goudah et al., 2008), birds (Patel
et al., 2008a, b), rabbit (Destache
et al., 2001) and mice (Ender et al.,
2003). Thus optimization of levofloxacin dose is required to ensure that
the judicious use of levofloxacin in food producing species such as cattle calves
without causing any toxicities to the animal as well to avoid public health
hazards. Therefore, the present study was taken up with the objective to evaluate
the pharmacokinetic profile of levofloxacin in cattle calves following an intravenous
and intramuscular administration at a dose of 10 mg kg-1.
MATERIALS AND METHODS
Experimental design: The study was conducted over a period of 6 months (October, 2009-March, 2010) on six healthy female cattle calves aging 3-6 months (weighing 47-85 kg) quarantined at the Dairy Farm of University. The cattle calves were maintained following standard managemental practices and provided concentrate, green fodder and wheat straw along with ad libitum clean and fresh drinking water. The experimental protocol was approved by the Institutional Animal Ethics Committee (IAEC).
The standard curve was prepared using pure technical grade levofloxacin from Sigma-Aldrich while injectable formulation of levofloxacin (Meriflox®; Wockhardt Ltd., Mumbai) was used for animal study. Levofloxacin was injected intravenously (i.v.) by jugular venepuncture and intramuscularly on the lateral aspect of neck and blood samples were collected into heparinised tubes by contralateral venepuncture at predetermined time intervals i.e., 0, 2.5, 5, 10, 15, 30, 45 min, 1, 1.5, 2, 3, 4, 6, 8, 12, 18 and 24 h post drug administration. The blood samples were centrifuged to separate plasma which were stored at -20°C until further assay.
Analytical procedure: Extraction of levofloxacin from plasma samples
was carried out as per the modified method of Nielsen and
Gyrd-Hansen (1997). The plasma (0.5 mL) was deproteinized by adding of 100
μL perchloric acid (0.6 M) into 0.5 mL plasma, vortexed for 1 min at high
speed and then centrifuged at 10,000 xg for 5 min. The supernatant was filtered
through millipore 0.22 μm filter and injected into HPLC system.
For determination of levofloxacin levels in plasma, an isocratic mobile phase
of acetonitrile, water, phosphoric acid and triethylamine (16:83:0.6:0.45, by
volume) with pH adjusted to 2.34 at a flow rate of 0.6 mL min-1 was
used to elute levofloxacin from C18-reverse phase column (4x125 mm;
particle size 5 μm, Waters XBridgeTM) which was detected using
a Waters 2996 photodiode array detector with software Empower at 294 nm (Gao
et al., 2007).
Peak areas were used for quantification of the compounds. The calibration curves prepared by spiking external standards in the pooled drug-free calf plasma were linear in the range of 0.02 to 3.2 μg mL-1 for levofloxacin with coefficient of variation (R2) of 0.98. Recovery was 90%. The lowest limit of detection and quantification were 0.01 and 0.02 μg mL-1, respectively. The intra and inter- assay coefficients of variation were 3.8 and 4.25, respectively.
Pharmacokinetic analysis: The plasma concentration vs. time data of
levofloxacin was analyzed with the help of a non-linear iterative curve fitting
computer programme (PHARMKIT) and other parameters were determined using the
equations described by Baggot (1977) and Gilbadi
and Perrier (1982). The best compartment model was selected on the basis
of positive and negative residuals and Akaike Information criterion. The dosage
regimens of levofloxacin were based on the pharmacokinetic and pharmacodynamic
indices of antimicrobials (Dudley, 1991; Walker,
Following intravenous administration, the mean plasma concentration of levofloxacin was 24.00±3.67 μg mL-1 at 0.04 h which initially declined to 9.65±0.60 μg mL-1 at 0.25 h and thereafter gradually to 2.40±0.13 μg mL-1 at 4 h (Fig. 1). Levofloxacin could be detected in plasma for up to 18 h after drug administration. An appreciable and clinically effective concentration of levofloxacin (1 μg mL-1) in plasma could be detected within at 0.04 h and the peak plasma concentration of levofloxacin was observed at 1 h following i.m. administration (Fig. 1). Levofloxacin could be detected in plasma up to 18 h after drug administration.
The intravenous and intramuscular plasma concentration-time data could be best
fitted to a two-compartment open model and one compartment open model, respectively
and were adequately described by the following biexponential equation:
CP = Ae-αt + Be-βt
for i.v. route
Cp = Be-βt - A'e-kat for i.m. route
where, Cp is the plasma concentration of levofloxacin at time t, A and B are the zero time plasma drug concentration intercepts of the biphasic disposition curve and α and β are first order rate constant related to distribution (or absorption) and elimination phases, respectively and e is base of the natural logarithm. The resulting pharmacokinetic parameters and pharmacodynamic-pharmacokinetic indices have been mentioned in Table 1 and 2.
||Semilogarithmic plot of plasma concentrations of levofloxacin
vs. time data following a single intravenous and intramuscular administration
of levofloxacin (10 mg kg-1) in goats, each point represents
the Mean±SE of 6 animals
||Pharmacokinetic parameters (Mean±SE) of a single intravenous
and intramuscular administration of levofloxacin (10 mg kg-1)
in cattle calves
|| Calculation of pharmacodynamic-pharmacokinetic indices for
dosage regimen computation
In the present study, the pharmacokinetic profile of levofloxacin following
its intravenous administration in cattle calves was in perfect accordance with
the two compartment disposition model. Two compartment pharmacokinetic behavior
of levofloxacin has already been reported in cross bred calves (Dumka
and Srivastava, 2006), sheep (Patel et al., 2012a),
buffalo calves (Ram et al., 2008, 2010),
goat (Ram et al., 2008; Mishra
and Roy, 2007), cats (Albarellos et al., 2005),
stallions (Goudah et al., 2008), birds (Patel
et al., 2008a, b), guinea pigs (Edelstein
et al., 1996), rabbit (Destache et al.,
2001) and mice (Ender et al., 2003).
A higher value of distribution rate constant (α) 20.20±6.22 h-1
indicated rapid distribution of levofloxacin in cattle calves. However, longer
distribution half lives have been reported in male camels (0.26±0.21
h), lactating goats (0.31±0.11 h) and stallion (0.21±0.13 h) (Goudah
and Abo-El-Sooud, 2009; Goudah et al., 2008;
Goudah, 2009). The results indicated that the distribution
of levofloxacin was faster in calves in comparison to camels, goats and stallion.
The differences may be attributed to the physiological variations among the
species including the variations in plasma protein binding of the drug. Variations
in the plasma microenvironment can significantly alter the bound fraction of
levofloxacin and thus result in major kinetic alterations (Sheikh
et al., 2001). Following single intramuscular administration at the
dose rate of 10 mg kg-1 b.wt. the absorption half-life (t1/2Ka)
following i.m. administration in calves was quite comparable to that reported
in cattle calves (0.35±0.03 h; Dumka and Srivastava,
2006), camels (0.43±0.23 h; Goudah, 2009)
and lactating goats (0.54±0.10 h; Goudah and Abo-El-Sooud,
2009); thus suggesting insignificant species variation in absorption kinetics.
The biological half life (t1/2β) of levofloxacin in cattle
calves following i.v. administration suggest rapid elimination of levofloxacin
from cattle calves as comparatively higher biological half life values have
been reported in camel and lactating goats (Goudah, 2009;
Goudah and Abo-El-Sooud, 2009). Elimination half-life
(t1/2β) of levofloxacin after i.v. and i.m. administration in
calves was almost comparable to that of 2.15±0.07 h in febrile sheep
(Patel et al., 2012b) and 3.05±0.17 h
in cross bred calves (Kumar et al., 2009), 3.27±0.31
h in buffalo calves (Ram et al., 2008), 3.47±0.86
h in camel (Goudah, 2009) and 2.94±0.78 h in
stallions (Goudah et al., 2008).
The AUC value of levofloxacin in cattle calves was similar to earlier reported
values in goats (Mishra and Roy, 2007), stallion (Goudah
et al., 2008). However, much lower value of AUC have been observed
in layer and broiler birds (11.07-11.33 μg h mL-1) after i.v.
administration (Patel et al., 2008a, b).
The AUC values for i.m. route were comparable to that of 21.31±1.24 μg
mL-1 h in lactating goats (Goudah and Abo-El-Sooud,
2009). Lower values have been observed in cross bred calves (7.66±0.72
μg mL-1 h) (Dumka and Srivastava, 2006),
buffalo calves (8.81±0.37 μg mL-1 h) (Ram
et al., 2008) and camels (13.63±3.11 μg mL-1
h) (Goudah, 2009).
The value of Mean Residence Time (MRT) of levofloxacin in cattle calves was
2.87±0.31 h and was much shorter compared to the MRT values in lactating
and nonlactating goats (Ram et al., 2008; Goudah
and Abo-El-Sooud, 2009; Mishra and Roy, 2007) and
camels (Goudah, 2009). The mean value of apparent volume
of distribution (Vdarea) was comparable to the volume of distribution
in febrile sheep (1.30±0.12 L kg-1) (Patel
et al., 2012b), cats (1.75±0.42 L kg-1) (Albarellos
et al., 2005), stallion (0.81±0.26 L kg-1) (Goudah
et al., 2008) but lower compared to that in layer birds (4.02±0.08
L kg-1) (Patel et al., 2008b). Based
on the results of the present study, it may be inferred that levofloxacin is
widely distributed into body tissues and fluids of cattle calves as is true
for fluoroquinolones in general in different species of animals (Brown,
1996; McKellar, 1996).
The ratio of transfer rate constant of the drug from the central to the peripheral compartment and vice versa (3.98±1.79) indicated a faster rate of drug transfer from the central to the peripheral compartment than redistribution from the peripheral to the central compartment. The fraction of drug in central compartment (fc) was 0.28±0.06 and the calculated value of T/P was (4.47±2.09). This was expected as the K12/K21 ratio was more than 1. Levofloxacin, as with several fluoroquinolones, is amphoteric because of the presence of a carboxylic acid and basic amine functional groups. Passive diffusion across biological membranes is a function of lipophilicity relative to the pKa values of the two ionizable moieties. These observations suggest that apart from wide distribution of drug from the central compartment, it is retained in peripheral tissues, a desirable feature for the treatment of deep seated infections.
Clearance of a drug indicates the volume of biological fluid (plasma/blood)
from which the drug has been removed per unit of time (L min-1 kg-1)
to account for its elimination. Total body clearance (ClB) is a measurement
of the ability of the body to eliminate drug and represent the sum of different
clearance processes of the drug from the body e.g., hepatic biotransformation
and renal excretion etc. Levofloxacin undergoes active renal tubular secretion
(Waheed et al., 2002). In the present study,
the clearance was comparable to earlier reports in sheep (Patel
et al., 2012a, b) but were much higher as
compared to human volunteers (Iqbal et al., 2000;
Waheed et al., 2002).
Bioavailability (F) is one of the important determinants of drug therapeutic
efficacy. In the present study, the overall bioavailability was 62.55±
5.99%. Lower bioavailability values have been reported to be in calves (44.3-56.6%)
in earlier studies (Dumka and Srivastava, 2006; Ram
et al., 2010). Much higher nonvascular bioavailability has been reported
in camels and sheep (90-94% approximately) (Goudah, 2009;
Patel et al., 2012a, b).
It has been established that for concentration-dependent fluoroquinolones,
the AUC/MIC ratio is the most important predictor of efficacy with a clinical
cure rate greater than 80% when this ratio is higher than 100-125 (Lode
et al., 1998). A second predictor of efficacy for concentration-dependent
antibiotics is the Cmax/MIC ratio, considering that values>10
lead to better clinical results (Toutain et al.,
2002). It has also been now accepted that high Cmax/MIC values
are necessary in order to avoid the emergence of bacterial resistance (Walker,
2000). The critical breakpoints that determine the efficacy of fluoroquinolones
are suggested as Cmax/MIC>0 and AUC/MIC>125 (Walker,
2000; Toutain et al., 2002). The MIC of levofloxacin
has not yet been determined for bacteria isolated from cattle calves. To cover
most of the susceptible organism, in the cattle population, the MIC90 of
0.06 to 0.12 μg mL-1 of levofloxacin has been taken into consideration
(Marshall and Jones, 1993). Based on the pharmacokinetic
data generated in this study and taking the extremes of MIC90, a
dosage of 10 mg kg-1 levofloxacin i.v. in cattle calves would result
in AUC/MIC and Cmax/MIC ratios in Table 2. Based
on the calculated Cmax/MIC and AUC/MIC, levofloxacin dose of 10 mg
kg-1 once daily can effectively be used by intravenous route of administration.
On the basis of the present study, a dose of 10 mg kg-1 of levofloxacin once daily intravenously or intramuscularly may be recommended for the effective eradication of bacterial pathogens in cattle calves.
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