First Report of Tracheliastes polycolpus (Copepoda: Lernaeopodidae) and Piscicola geometra L. 1761 (Annelida-hirudinea) on Capoeta umbla at Murat River, Turkey
This study investigated the presence of lernaeopodid and parasitic annelids on Capoeta umbla from Murat River. The host fish was found to be infested with the ectoparasite Tracheliastes polycolpus (Copepoda: Lernaeopodidae) and Piscicola geometra (Hirudinida: Pontobdellinae). Parasites were isolated from the fins of C. umbla that the most common fish species of Murat River. The present report constitutes the first diagnosis of T. polycolpus in Turkey and P. geometra known from different freshwater sources in Turkey. The morphological structure of two parasites were measured and photographed.
Received: March 16, 2011;
Accepted: June 25, 2011;
Published: August 08, 2011
The Turkish parasitological literature contains very scant data on Capoeta
umbla parasites. The most recent survey of C. capoeta umbla parasites
in Turkey was carried out by Aksoy et al. (2006)
and Saglam (1992) T. polycolpus on Capoeta
trutta in Keban Dam Lake.
T. polycolpus and P. geometra were reported in several countries
affecting freshwater fishes. Piscicola geometra was also previously reported
from nine host species in Turkey, Rutilus rutilus, Blicca bjoerkna,
Esox lucius, Tinca tinca (Ozturk, 2002; Karatoy,
2004; Akbeniz and Soylu, 2008), Barbus rajanorum
mystaceus (Saglam, 1992), Abramis brama, Scardinius
erythrophthalmus (Karatoy, 2004), Cyprinus carpio
(Oktener et al., 2007) and C. gibelio
(Arslan and Emiroglu, 2011).
T. polycolpus was reported from Iran, on Capoeta trutta at Vahdat
Res, Tigris region Barzegar and Jalali (2009) and on
Capoeta capoeta at Mahabad Res and Zarineh-rud Ri, Uromia region Mirhashemi
Nasab and Pazooki (2003). This record complies with present study. The prevalence
of C. umbla infection with P. geometra was 2.34% but the prevalence
of T. polycolpus can be negligible.
The aim of this study was to existence of T. polycolpus in Murat River in study sites, among different fin microhabitats within one host species, C. umbla. The other hand first record of P. geometra on C. umbla in Murat River.
MATERIALS AND METHODS
Copepod and Leeches were found on C. umbla in Murat River that is a
long river (722 km) in South East Anatolia of Turkey, the sampling area in this
study were located near Bingöl city (38.8 N, 41.05 E). A total of 128 C.
umbla were collected from Murat River. Collections were made using fishing
net. After capturing, the fish specimens were placed in plastic tank with river
water and transferred to the research laboratory where they were kept in an
aquarium. The fish were anaesthetized and measured to the nearest millimeter
(total body length). Host fish individuals were examined for ectoparasites from
spring 2010 to spring 2011. T. polycolpus and P. geometra were
found on anal fin of C. umbla and removed with aid of preparation needle.
T. polycolpus identification was performed according to Bychowskaya-Pavlovskaya
(1962) and identifications of P. geometra were performed according
to Burreson (1995). Parasitic samples were killed in
hot (not boiled) 4% formaldehyde solution and were preserved in 70% ethanol.
All samples were cleared in lacto-phenol. The leech specimens and copepod collected
are deposited in the personal collection of M. KOYUN and the collection of the
Department of Zoology University Bingol University Biology department. The parasite
specimens were photographed with a trinokuler stereo microscope (Fig.
|| Tracheliastes polycolpus
|| Piscicola geometra (L, 1z761)
|| P. geometra on anal fin of C. umbla (Host fish)
RESULTS AND DISCUSSION
Despite a lot of fish in the examination, four leech specimens and only one specimens lernaeopodid copepod were found from the Murat River. Both parasites were found at Summer time.
In present study, we focused on the lernaeopodid copepod T. polycolpus (Von Nordmann 1832) and P. geometra L. 1761 an ectoparasite on the fins of C. umbla (cyprinid fish).
T. polycolpus carried by two long second maxillae united only at their
distal end, is characterized by a short manubrium with a disk shaped anchor
with attaches itself to fin ray (Benkirane et al.,
The copepod (T. polycolpus) that recoded in our study was adult female, it is anchored to host fins and feeds on the epithelial cells and mucus of the host, characteristically raising blisters on the fin surface.
The present report is the first diagnosis of C. umbla in Turkey. However, there were some records about these two parasites on various freshwater fishes but ours is the first record on C. umbla in Murat River.
T. polycolpus (von nordmann 1832): T. polycolpus is differentiated
by examination of both the cephalothoraxes and trunk which are elongate and
tubular (Fryer, 1982). The specimens was sexually mature
female with 5.29-0.21 mm total body length and was attached to the host with
a wide, disk shaped bulla. Egg was scattered the body surface (Fig.
Piscicola geometra (L, 1761): The body structure is cylindrical and long and contains two pairs of suction cups to the anterior eye. Posterior suction cups are connected to the body as external-based. The radial pigment lines are as an alternative to the points of eye. The lateral vesicles are 11 pairs, narrow, open, longitudinal bands extending from the middle of the ventral surface transverse and cut by open metameric bands. Star- shaped pigments cells are usually seen. All four anulliden rings were present. Parasite length 23 mm, the width was measured as 3.5 mm Fig. 2.
During the study Fish Samples collected along the Murat River, only infested
fish were found at spring and summer time. The copepod showed an aggregated
dispersion pattern on host fins. The results showed significantly preferred
microhabitats, with adult females being more abundant on the anal pelvic fins
than the other fins. In particular, Loot et al. (2004)
found that it was more abundant on the anal and pelvic fins than the other.
T. polycolpus were never found on caudal and dorsal fins and were relatively
scarce on the body surface of fish. These fin alterations may reduce the fish's
swimming ability and therefore affect the rostrum dace population (Loot
et al., 2004). The anal fins were damaged by the T. polycolpus
with a loss of their surface area Fig. 3. For this reason,
bacteria, fungi and other parasitic agents can easily attack and settle down
and especially the ecologies and biologies, seasonal distributions and their
effects on fish of these two parasites can be studied in subsequent studies.
Even though P. Geometra records have been presented in previous studies
in some fish species of Cyprinidae which is of Turkeys freshwater fish
(Cyprinus carpio, Capoeta trutta, Rutilus rutilu,s Barbus
rajanorum mystaceus, Blicca bjoerkna, Esox lucius, Tinca
tinca, Abramis brama, Scardinius erythrophthalmus), it is
seen that those remain limited. P. geometra record is found only in C.
trutta of Capoeta fish. T. polycolpus record is found in Capoeta
trutta only by Saglam (1992) master thesis titled
Ecto Parasites of Some Cyprinid Fish in Keban Dam Lake and there is not other
record apart from this. Therefore, that both two parasites will be the first
record both for C. umbla and Murat River is seen important.
The author is very grateful to Prof. Dr. Naim SAGLAM from Department of Aquaculture and Fish Diseases, Faculty of Fisheries Elazýg-Turkey for his verification for of P. geometra and T. polycolpus.
1: Akbeniz, E. and E. Soylu, 2008. Metazoan parasites of tench (Tinca tinca L., 1758) in the lake sapanca, Turkey. J. Fish. Aquatic Sci., 23: 13-18.
Direct Link |
2: Aksoy, S., N. Saglam and M. Dorucu, 2006. External parasites of three cyprinid fish species from lake Hazar in Turkey. Indian Vet. J., 83: 100-101.
Direct Link |
3: Arslan, N. and O. Emiroglu, 2011. First record of parasitic annelida-hirudinea (Piscicola geometra Linnaeus, 1761) on Carassius gibelio (Bloch, 1782) inLake Uluabat (Turkey). Kafkas Univ. Vet. Fak. Derg., 17: 131-133.
CrossRef | Direct Link |
4: Barzegar, M. and B, Jalali, 2009. Crustacean parasites of fresh and brackish (Caspian Sea) water fishes of Iran. J. Agric. Sci. Technol., 11: 161-171.
Direct Link |
5: Benkirane, O., F. Coste and A. Raibaut, 1999. On the morphological variability of the attachment organ of lernaeopodidae (Copepoda: Siphonostomatoida). Folia Parasitol., 46: 67-75.
Direct Link |
6: Bychowskaya-Pavlovskaya, I.E., 1962. Key to Parasites of Freshwater Fish of the USSR. In: Moskva-Leningrad: Izdatel-stvo Akademii Nauk SSR, Pavlovskii, E.N. and B.E. Bykhovskii (Eds.). English Translation-Israel, Jerusalem, pp: 919.
7: Burreson, E.M., 1995. Protozoon and metazoon ınfections. Fish Dis. Disorders, 1: 599-629.
8: Fryer, G., 1982. The Parasitic Copepoda and Branchiura of British Freshwater Fishes: A Handbook and Key. Freshwater Biological Association, Ambleside, Cumbria.
9: Karatoy, E., 2004. Metazoan parazites of bream (Abramis brama Linnaeus, 1758) in the Lake Durusu (Terkos). M.Sc. Thesis, Institute of Science Marmara University.
10: Loot, G., N. Poulet, Y. Reyjol, S. Blanchet and S. Lek, 2004. The effects of the ectoparasite Tracheliastes polycolpus (Copepoda: Lernaeopodidae) on the fins of rostrum dace (Leuciscus leuciscus burdigalensis). Parasitol. Res., 94: 16-23.
11: Mirhashemi Nasab, S.F. and J. Pazooki, 2003. Identification of crustacean parasites in some fishes of mahabad reservoir (in Persian). Iran. J. Fish. Sci., 11: 133-148.
12: Oktener, A., J.P. Trilles and I. Leonardos, 2007. Five ectoparasites from Turkish fish. Turk. Parazitol. J., 31: 154-157.
PubMed | Direct Link |
13: Ozturk, M.O.G., 2002. Metazoan parasites of the tench (Tinca tinca L.) from Lake Uluabat, Turkey. Israel J. Zool., 48: 285-293.
Direct Link |
14: Saglam, N., 1992. some external parasites on cyprinids in Keban Dam Lake. Master Thesis, Firat University, Science Institution, Elazig.