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Research Article

Plasma Biochemical Changes in Clarias gariepinus (Burchell, 1822) Fed Poultry Litter

Bamidele Oluwarotimi Omitoyin
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Clarias gariepinus juveniles were fed for twelve weeks with poultry litter in other to assess its effect on biochemical parameters as bio-indicator of health status. A control experiment was set up with fish fed a normal 40% crude protein diet. Fish fed with poultry litter shows a significant increase (p<0.05) in the values of plasma electrolyte of sodium, chloride, phosphate: excretory products of urea and creatinine; globulin and albumin/globulin ratio compared with initial values and fish fed the control diet which is suggestible of abnormal physiological function of metabolism in fish fed with poultry litter when compared with fish fed control diet. While significantly lower (p<0.05) levels of potassium, bi-carbonate and triglycerides observed in fish fed poultry litter is an indication of lower immune level of fish compared with fish fed control diet. The present study shows that feeding fish with poultry litter reflect disturbance in metabolism activities, hence it should not be used as a complete feed for C. gariepinus.

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Bamidele Oluwarotimi Omitoyin , 2007. Plasma Biochemical Changes in Clarias gariepinus (Burchell, 1822) Fed Poultry Litter. Asian Journal of Animal Sciences, 1: 48-52.

DOI: 10.3923/ajas.2007.48.52



The aim of this study is to investigate the effects of feeding poultry litter on plasma biochemical parameters of Clarias gariepinus juveniles.

Aquaculture is one of the fastest growing food production sectors in the world and provides significant supplement and substitute to wild fishes (Francis et al., 2001). The aquaculture sector is confronted with the problem of high cost and irregular supply of conventional fish feed ingredients. However, Omitoyin (1995) and Aderemi et al. (2004) observed that majority of feed ingredients required for the animal feeds can be met by using agro-industrial products, which are considered as wastes. The ingestion of a number of dietary components however has measurable effects on blood constituents of various animals (Church et al., 1984; Arowora et al., 2003). Blood contains a lot of metabolites and other constituents, which provide a valuable medium for clinical investigation and nutritional status of the individual (Heming and Paleczny, 1987; Arowora et al., 2003). Blood is also a good indicator to determine the health of an organism (Joshi et al., 2002a). Taiwo et al. (2003) reported negative impact on the growth of C. gariepinus broodstock fed grasscutter faeces.

Hilmy et al. (1987) observed an increase in protein content of serum and liver in fish following cadmium and zinc toxicity. Everall et al. (1991) reported similar change in adult captive Atlantic salmon.

In Nigeria many semi-intensive fish farmers particularly in the rural and peri-urban cities are faced with the problem of availability of cost effective fish feed (Omitoyin, 2005). In other to solve this problem, many of these fish farmers feed their fish directly with poultry litter in addition to other forms of supplementary feed. There is therefore the need to investigate the effect of this direct feeding of fish with poultry litter which is a major waste by product from the poultry industry in Nigeria.


Experimental Fish and Management
This study was conducted between July and October 2005. One hundred twenty pieces of Clarias gariepinus juveniles of average length (11.0±1.5 cm) and weight (15.70±0.1) were purchased from a commercial fish farm in Mokola, Ibadan Nigeria. The fish were disinfected with 0.1% potassium permanganate as described by Joshi et al. (2002b) and were acclimatized for 14 days to laboratory condition in a glass aquaria size 7x0.5x0.45 m in the research laboratory of the Department of Wildlife and Fisheries Management, University of Ibadan, Nigeria. The fishes were fed with standard commercial cat fish diet (40% crude protein). After acclimatization, the fishes were divided into two treatments with three replicates in six 90x60x30 cm glass aquaria. Eighteen fishes were randomly distributed into each aquarium. The test fish were fed for 12 weeks with dried poultry litter collected from the battery cage system of the Teaching and Research farm of the University of Ibadan. The litter was sun-dried for two days before grinded and pelleted, while the control group were fed with 40% crude protein commercial catfish diet at 3% of their body weight.

Water quality parameters such as temperature, dissolved oxygen and pH of the experimental set up were monitored using standard methods (APHA, 1998; Boyd, 1979). While the proximate composition of poultry litter and control diets were carried out using the AOAC (1990).

Plasma Biochemical Evaluation
Blood samples of the Clarias gariepinus juveniles at the beginning of feeding trial and after twelve weeks of feeding with poultry litter and control diet were collected from un-anaesthetized fish by cardiac puncture with a 1.4x50 mm2 non-heparinized injection needle as described by Morgan and Iwama (1997) and Abdelmeguid et al. (2002). Plasma sodium and potassium were analyzed in the blood samples by using flame emission photometry (Morgan and Iwama, 1997). Plasma total proteins were estimated through the biuret method (Reinholf, 1953). The plasma urea, triglycerides, creatinine, globulin and albumin/globulin ratio by the standard methods described by Coles (1986), while cholesterol was determined by the direct method described by Hrubec et al. (1996). Plasma chloride was determined by using mercuric nitrate method according to Schales and Schales (1941). Calcium, phosphate and bicarbonate by Toro and Ackmann (1975).

Water Quality Parameters
The following water quality parameters were determined: Dissolved oxygen, pH and temperature respectively using Lamottee freshwater aquaculture test kit model AQ-2/AQ-3 codes 3633-03/3634-03 (2003).

Statistical Analysis
Experimental design was one-way analysis of variance (Steel et al., 1997) and mean differences were carried out according to Duncan multiple (1957) range test.


There were no significant differences in water quality parameters measured during the experimental period as presented in Table 1.

Table 1: Water quality parameters of experimental set up
Image for - Plasma Biochemical Changes in Clarias gariepinus (Burchell, 1822) Fed Poultry Litter

Table 2: Plasma bio-chemical changes in Clarias gariepinus fed poultry litters
Image for - Plasma Biochemical Changes in Clarias gariepinus (Burchell, 1822) Fed Poultry Litter
Mean with the same superscripts along the horizontal row are not significantly different (p>0.05)

Table 3: Proximate analysis of experimental diets
Image for - Plasma Biochemical Changes in Clarias gariepinus (Burchell, 1822) Fed Poultry Litter
Means with the same alphabets along the vertical roll are not significantly different (p>0.05)

The values of sodium, chloride, phosphate, urea, globulin and albumin/globulin ration of fish fed poultry litter was significantly different (p<0.05) from the initial values and those fed the control diet (Table 2). However both the initial values of phosphorous and bicarbonate and that of fish fed control diet were significantly (p<0.05) better than fish fed poultry litter. Poultry litter has a significantly (p<0.05) lower nutritional value compared to the control diet (Table 3).


Water quality parameters of experimental set-up for both treatments were similar and within the optimum range recommended for culture of C. gariepinus (Viveen et al., 1985; Omitoyin et al., 2006).

The increase in the value of plasma electrolytes, renal excretory products of urea and creatinine observed in this study in the fish fed poultry litters compared to initial values and those fed the control diet are suggestive of abnormal physiological function of metabolism. However, the higher plasma electrolyte, total protein and albumin/globulin ratio, values of test fish is an indication of efficient immune responses and body physiological reaction to poultry litters. While reductions in serum albumin and increases in globulin levels i.e., alterations in albumin/globulin ratio may result from the poorer liver function as well as proteinuria due to kidney damage (Uyanik et al., 2001) characterized by degeneration in tubular epithelium as well as hyaline casts in the lumen. Since the albumin levels decreased, the increases in globulin levels may elevate the total protein as suggested by Uyanik et al. (2001). The increase in total protein and globulin levels as reported by Chapatwala et al. (1982a), Chapatwala et al. (1982b) and Uyanik et al. (2001) may depend on dehydration due to diminished feed and water intake. The lower level of bicarbonate, Calcium Globulin and Triglyceride indicated the deficiency of hepatic metabolites compound as well as abnormal physiological function of the metabolism and indicates the low immune level of fish fed with poultry litters compared with fish fed control diet which was similar to the observation of Tiwari and Singh (2005).

The results reported for serum proteins are in agreement with those obtained by Mckim et al. (1970) and Abdelmeguid et al. (2002). The increase in serum creatinine concentration in fish fed with poultry litters in comparison with those fed control diet and initial values might be induced by glomerular insufficiency, increased muscle tissue catabolism or impairment of carbohydrate metabolism as reported by Murray et al. (1990) and Abdelmeguid et al. (2002) respectively. Proximate composition of the poultry litter fed to catfish also revealed that it was less than half of the optimum level of 40% C.P when compared with that of control diet as recommended by Faturoti et al. (1986a).

It is therefore evidence from this study that disturbance in blood serum as a result of feeding fish with poultry litters reflect disturbance in metabolism activities, hence poultry litters should not be used as a complete feed for C. gariepinus.

Further study should be carried out on partial utilization of poultry litters as feed component in the diet of C. gariepinus and other fish species in aquaculture production rather than as a complete feed.


1:  Abdelmeguid, N., A.M. Kheirallah, Abou-Shabana, K. Adham and A. Abdel-Moneim, 2002. Histochemical and biochemical changes in liver of Tilapia zilii G. as a consequence of water pollution. J. Biol. Sci., 2: 224-229.
CrossRef  |  Direct Link  |  

2:  Aderemi, F.A., O.A. Ladokun and O.O. Tewe, 2004. Study on haematology and serum biochemistry of layers fed biodegraded cassava root sieviate. Bowen J. Agric., 1: 79-83.
Direct Link  |  

3:  AOAC., 1990. Official Method of Analysis. 15th Edn., Association of Official Analytical Chemists (AOAC), Washington, DC., USA

4:  APHA, 1998. Standards Methods for the Examination of Water and Waste Water. 20th Edn., American Public Health Association, Washington, DC., USA

5:  Arowora, K.A., A.A. Onilude and O.O. Tewe, 2003. Seral, haemotological and economics of production of weaner pigs fed graded levels of biodegraded cassava (Manihot esculenta) peels. Trop. J. Anim. Sci., 6: 7-16.
Direct Link  |  

6:  Boyd, C.E., 1979. Water Quality in Warm Water Fish Ponds. 1st Edn., Craft Master Printers, Albama

7:  Chapatwala, K.D., M. Hobson, D. Desaiah and B. Rajanna, 1982. Effect of cadmium on hepatic and renal gluconeogenic enzymes in female rats. Toxicol. Lett., 12: 27-34.
CrossRef  |  Direct Link  |  

8:  Chapatwala, K.D., M. Boykin, A. Butts and B. Rajana, 1982. Effect of intraperitoneally injected cadmium on renal and hepatic gluconeogenic enzymes in rats. Drug Chem. Toxicol., 5: 305-317.
CrossRef  |  Direct Link  |  

9:  Church, J.P., J.T. Judd, C.W. Young, J.L. Kelsay and W.W. Kim, 1984. Relationships among dietary constituents and specific serum clinical components of subjects eating self-selected diets. Am. J. Clin. Nutr., 40: 1338-1344.
Direct Link  |  

10:  Coles, E.H., 1986. Veterinary Clinical Pathology. 4th Edn., W.B. Saunders Company, Philadephia, pp: 10-42

11:  Duncan, D.B., 1957. Multiple range tests for correlated and heteroscedastic means. Biometrics, 13: 164-176.
CrossRef  |  Direct Link  |  

12:  Everall, N.C., C.G. Mitchell, D.B. Groman and J.A.A. Johnston, 1991. Tracing of haematoxic agents in water with captive adult atlantic salmon (Salmo salar L.) in the River Don; Aberdeen Shire. Dis. Aquat. Org., 10: 75-85.

13:  Faturoti, E.O., A.M. Balogun and L.L.C. Ogwu, 1986. Nutrient utilization and growth responses of Clarias lazera fed different dietary protein levels. Niger. J. Applied Fish. Hydrobiol., 1: 41-45.

14:  Francis, G., H.P.S. Makkar and K. Becker, 2001. Antinutritional factors present in plant-derived alternate fish feed ingredients and their effects in fish. Aquaculture, 199: 197-227.
CrossRef  |  Direct Link  |  

15:  Heming, T.A. and E.J. Paleczny, 1987. Compositional changes in skin mucus and blood serum during starvation of trout. Aquaculture, 66: 265-273.
CrossRef  |  Direct Link  |  

16:  Hilmy, A.M., N.A. El-Domiaty, A.Y. Daabees and H.A. Abdel-Latief, 1987. Some physiological and biochemical indices of zinc toxicity in two freshwater fishes. Clarias lazera and Tilapia zilii. Comp. Biochem. Physiol., 87: 297-301.
PubMed  |  

17:  Hrubec, T.C., S.A. Smith, J.C. Robertson, B. Fieldman, H.P. Velt, G. Lisbey and M.K. Tinker, 1996. Blood biochemical reference interval for sunshine bss in three culture systems. Am. J. Vet. Res., 1: 624-627.
Direct Link  |  

18:  Joshi, P.K., M. Bose and D. Harish, 2002. Changes in certain haematological parameters in a suliroid catfish Clarias batrachus (Linnaeus) exposed to cadmium chloride. Pollut. Resour., 21: 129-131.

19:  Joshi, P.K., D. Harish and M. Bose, 2002. Effect of lindane and malathion exposure to certain blood parameters in a fresh water teleost fish Clarias batrachus. Pollut. Resour., 21: 55-57.

20:  Mckim, J.G., M. Christensen and E.P. Hunt, 1970. Changes in the blood of brook trout (Salvelinus fontinalis) after short-term and long-term exposure to copper. J. Fish Res. Breed. Can., 27: 1883-1889.
CrossRef  |  Direct Link  |  

21:  Morgan, J.D. and G.K. Iwama, 1997. Measurement of Stress State in the Field. In: Fish Stress and Health in Aquaculture, Iwama, G.K., A.D. Pickering, J.P. Sumpter and C.B. Schreck (Eds.). Published by Cambridge University Press, Trumpington Street, Cambridge, pp: 247-268

22:  Murray, R.K., D.K. Granne, P.A. Mayes and V.W. Rodwell, 1990. Harper`s Biochemistry. 23rd Edn., Appleton and Large Publishers, Los Altos, California

23:  Omitoyin, B.O., 1995. Utilization of poultry by products (feather and offals) in the diets of African catfish Clarias gariepinus (Burchell). Ph.D Thesis, University of Ibadan, Ibadan Nigeria, pp: 219.

24:  Omitoyin, B.O., 2005. Problems and prospects of fish feed production in Nigeria. Invited Technical Paper Presented at the USAID Aquaculture Marketing Stakeholder Forum Held at University of Ibadan Conference Center on 13th December 2005, pp: 3.

25:  Omitoyin, B.O., E.K. Ajani, B.T. Adesina and C.N.F. Okuagu, 2006. Toxicity of lindane (Gamma Hexachloro-Cyclottexane) to Clarias gariepinus (Burchell, 1822). World J. Zool., 1: 57-63.

26:  Reinholf, J.D., 1953. Standard Method of Clinical Chemistry. Academic Press, New York

27:  Schales, O. and S.A. Schales, 1941. A simple and accurate method for the determination of chloride in biological fluids. J. Biol. Chem., 140: 879-884.
Direct Link  |  

28:  Steel, R., J. Torrie and D. Dickey, 1999. Principles and Procedures of Statistics: A Biometrical Approach. 3rd Edn., McGraw-Hill, New York, USA
CrossRef  |  Direct Link  |  

29:  Taiwo, V.O., B.O. Omitoyin, A.O. Ogunsami, O. Olukunle and K.A. Ogunmoye, 2003. Toxicity of grasscutter (Thryonomys swinderianus Temmick) faeces to Clarias gariepinus broodstock. J. Trop. For. Res., 19: 23-32.

30:  Tiwari, S. and A. Singh, 2005. Possibility of using latex extracts of Nerium indicum plant for control of predatory fish Channa punctatus. Asian Fish. Soc., 18: 161-173.

31:  Toro, G. and P. Ackermann, 1975. Practical Clinical Chemistry, 1st Edn., Little Brown and Company, Boston, pp: 237-238

32:  Uyanik, F., M. Eren, A. Afasever, G. Tuncoku and A.H. Kolsuz, 2001. Changes in some biochemical parametesr and organs of broilers exposed to cadmium and effect of zinc on cadmium induced alterations. Israel J. Vet. Med., 56: 128-134.

33:  Viveen, W.J.A.R., C.J.J. Rither, P.G.W.J. Van Oordt, J.A.L. Janssen and E.A. Huisman, 1985. Practical manual for the culture of the African catfish (Clariasgariepinus). Directorate General for International Technical Corporation, Hague, Netherlands, pp: 93

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