Performance and Egg Quality Characteristics of Laying Birds Fed Diets Containing Rice Bran with and Without Yeast Supplementation
A total of 120 birds were divided into 5 groups of twenty four birds each. Each group was regarded as treatment. The treatment was subdivided into 3 groups of 8 birds each as replicates. Treatment 1 served as control diet with no rice bran and yeast supplementation, diet 2 and 3 contained 30 and 35% rice bran without yeast supplementation, respectively. Diets 4 and 5 were the same as diets 1 and 2 except that 2 g kg-1 of yeast was added to each. Between 45th and 50th week of age of the birds egg quality trial was conducted to assess haugh unit, shell thickness and yolk index. Results of feed intake, hen day egg production, feed conversion ratio and mortality were not significantly (p<0.05) influenced by dietary treatments. Haugh unit, yolk index and average weight of egg did not vary significantly (p<0.05) between treatments, however, shell weight and shell thickness were significantly affected by the treatments. Total cost of production was higher (p<0.05) for the control group compared to treatments 2, 3 and 5. Revenue generated from sale of eggs was higher for birds on treatment 4 compared to those on treatments 1 and 5 (p<0.05). Net farm income was higher for treatments 2, 3 and 4 compared to the control. It could be concluded from the results of this study that performance of birds was not affected by the levels of rice bran with and without yeast supplementation. However, shell thickness, shell weight and net farm income were significantly affected by the treatments.
The use of agro-industrial by-products in layers diets has been well documented (Wang, 1997; Odunsi et al., 1999; Onifade et al., 2000). Rice bran is one the most abundant agro-industrial by-product found in the study area (Sokoto state, Nigeria) as rice constitute major part of the staple food. It is largely used as animal feed (Wang, 1997) that is rich in B vitamins, fat and protein and compares favorably with other cereal grains in amino acid composition (Warren and Farrell, 1991). However it has high content of fiber (Warren and Farrell, 1990; Farrell, 1994) that is rich in the hemicelluloses containing highly branched arabinoxylans (Erbingerova et al., 1994). Yeast has been reported to increase the nutritive quality of feeds and performance of animals (Kumar and Dingle, 1996; Onifade and Babatunde, 1996; Abubakar et al., 2004). The present study attempted to take advantage of supplementing rice bran containing diets with yeast in order to come up with cheaper layer rations without compromising performance.
MATERIALS AND METHODS
The study was conducted at the Teaching and Research Unit of the Department of Animal Science located along Aliyu Jodi Road Sokoto, Sokoto State, Nigeria.
The trial commenced at the 41st week and lasted for 12 weeks i.e., up to the
52nd week of age. A total of 120 birds of Shika brown strain were divided
into 5 groups (treatments of 24 birds each). Each treatment was subdivided into
three replicates of 8 birds each. Each treatment was assigned to one of the
following experimental diets: Diet 1: control- no rice bran, no yeast; Diet
2: 30% rice bran, Diet 3: 35% rice bran. Diets 4 and 5 were the same as diets
1 and 2 except that they contain 2 g kg-1 of yeast. The gross composition
of the diets is shown in Table 1 while the calculated chemical
composition of the diets is presented in Table 2.
All the diets contained similar crude protein and energy levels. However crude
fiber varied from 4.3% for the control to 13 and 15% for the low and high rice
bran containing diets (Table 2).
Records of feed intake, egg production and mortality were taken. Feed conversion ratio in terms of number of eggs produced per kg of feed consumed, kg of egg produced per kg of feed consumed and dozen of eggs produced per kg of feed consumed were calculated from records of feed intake and egg production.
Egg Quality Assessment
Between the 45th and 50th weeks of age, egg quality assessment was carried
out. Haugh unit, yolk index albumen index and shell thickness were determined.
To determine these parameters, 5% of the total eggs laid by each group during
five consecutive days of the trial were weighed using a sensitive electronic
||Gross composition (%) of layers diets containing different
levels of rice bran (RB) and yeast (Y)
||Calculated chemical composition (%) of the layers diets containing
different levels of rice bran (RB) and yeast (Y)
|**Based on prices of ingredients as at November 2002
After weighing, each egg was broken with a sharp knife and gently emptied into
a petri dish. Using Venire Caliper, yolk height and diameter, as well as albumen
height were measured from three different positions (broad end, narrow end and
center) as described by Panda et al. (2003).
These measurements were used to calculate Haugh unit and yolk index. Shell
weight was measured using electronic scale while shell thickness was measured
with a micrometer screw gauge. Haugh unit was calculated by taking the average
values of albumen height and weight of the eggs using the formula below (Oluyemi
and Roberts 2000):
||Albumen height (mm
||Observed weight of eggs (g)
The yolk index was calculated using the formula below:
Egg mass was calculated by dividing the total weight of eggs laid by the total number of birds.
All data were subjected to analysis of variance using SAS (1990) following
completely randomized design and means separation was done by Duncan Multiple
Range Test (DMRT) as outlined by Steel and Torrie (1980).
Table 3 shows the results of the laying trial. Total feed
intake varied from 8.2 kg for treatment 2 to 9.3 kg for treatment 5, with no
significant differences between the treatments. The average daily feed intake
followed a similar pattern (Table 3). Hen day egg production
(HDE) of birds was similar (p>0.05) for all treatments despite the variation
between 58% for treatment 5 to 64% for treatment 4. On the contrary hen housed
egg production (HHE) was significantly higher ((p<0.05) for treatments 4
compared to treatments 1 (54%), 3 (55%) and 5 (55%). Feed conversion ratios
were similar for all treatments.
Cost of feed consumed per bird differed significantly (p<0.05) between the treatments. It decreased from N210 for birds on the control diet to N158 for birds on diet 2. The value obtained for the control diet was higher (p<0.05) compared to those observed for treatments 2, 3 and 4.
The highest mortality was recorded for birds on treatments 1 and 3 (5% each).
For treatments 2 and 4, mortality was 4%, while no death was recorded for birds
on treatment 5. These variations were however not significant (p>0.05) (Table
Egg Quality Assessment
Results on egg quality assessment are presented in Table 4.
Albumen height did not show any significant difference (p>0.05) between the
treatments, although higher values were recorded for treatments 4 (5.9 mm) and
5 (5.5 mm) compared to treatments 1 (5.3 mm), 3 (5.4 mm) and 2 (5.7 mm).
||Performance of laying pullets fed different levels of rice
bran (RB) and yeast (Y) between 41-52 weeks of age
|abcdeMeans along the same row with different superscripts
are significantly (p<0.05)
||Egg quality of laying birds fed diets containing different
levels of rice bran (RB) and (Y)
|abcMeans along the same row with different superscripts
are significantly (p<0.05)
Yolk index did not also differ between the treatments even though the control
group recorded the highest value of 3.83 compared to the other treatments.
Shell thickness was higher (p<0.05) for birds on treatment 4 (0.39 mm) compared to those on treatments 1, 3 and 5. The other treatments did not differ significantly in shell thickness. Shell weight was higher for treatment 3 (13.8 g) compared to treatments 1 (13.4 g) and 4 (13.1 g) (p<0.05). Haugh unit did not differ significantly between the treatments, even though the highest value was recorded for birds on diet 2 (91%) and the least was recorded for birds on the control and treatment 3 (87%).
Egg mass (g per bird/day) varied slightly from 33 g for treatment 2 to 36 g
for treatment 4 (p>0.05). Egg weight averaged 57 g/bird across the treatments
Cost Analysis of Feeding Experimental Diets to the Laying Birds
Total variable cost (Nbird-1) (that is cost of feed, medication,
labor and empty crates) was higher (p<0.05) for treatments 1 (N1,094) and
4 (1,050) compared to treatments 2 (N963) and 3 (N962) (Table
5). Total fixed cost (i.e., cost of chicks and housing) was similar (p>0.05)
for all treatments (N233 bird-1).
Total cost of production (fixed and variable) was higher (p<0.05) for the
control group (N1, 327) compared to treatments 2 (N1,205), 3 (N1,195) and 5
(N1, 23). Total cost of production did not differ significantly between the
other treatments (Table 5). Revenue generated from sale of
egg eggs was higher for birds on treatment 4 (N1,761) compared to those on treatments
1 (N 1,553) and 5 (N 1,558) (p<0.05). The other treatments did not differ
significantly in this parameter.
||Cost analysis of raising layers on the experimental diets
containing different levels of rice bran (RB) and yeast (Y)
|abMeans along the same row with different superscripts
are significantly different (p<0.05); *Mortality was not taken into consideration.
(It varied from 4-5 % for all treatments (p>0.05) and could not be ascribe
to the treatments)
Revenue from sale of spent layers and litter was the same for all treatments.
Total revenue was higher (p<0.05) for treatment 4 (N2281) compared to treatments
1 (N2074) and 5 (N2078) (Table 5). Net farm income was higher
for treatments 2 (N952.84), 3 (N972.03) and 4 (N997.83) compared to the control
Neither the level of rice bran nor yeast supplementation had any significant
effect on feed intake. The results however showed that yeast supplementation
led to non-significant increase in feed intake at both levels of rice bran incorporation.
This could be due to the flavor-enhancing characteristic of yeast, as observed
by Martin et al. (1989). Nahashon et al. (1996) also reported
increased feed intake when diets of laying birds were supplemented with microbial
enzymes. It has long been recognized that yeast increases feed intake in animals
(Günter, 1989; Wallace, 1997), probably due to its ability to produce glutamic
acid, which has flavor enhancing properties (Lyons, 1987; Rose, 1987). Average
daily feed intake of birds on all treatments was similar throughout the 3 months
of the trial (96-111 g/bird/day). Adamu and Ubosi (1998) reported lower values
of 92.78-98.53 g/bird/day. However, Abdullahi (2004) reported higher values
of 141.77 to 156.6 g/bird/day for laying birds aged 42-59 weeks.
The similar feed intake recorded across the treatments could be due to the uniform energy contents of the diets. Birds are known to eat to satisfy their energy requirements (Gietema, 1992). Therefore where energy values are similar, feed intake will not be expected to vary.
Feed conversion ratio was not significantly influenced by either levels of rice bran or yeast addition to the diets. However, yeast supplementation tended to decrease feed conversion efficiency at the high incorporation level of rice bran. This could be attributed to higher feed intake without concomitant increase in egg production. The feed conversion ratios reported in this trial were within the range of 1.5-3.15 kg of feed consumed kg-1 of egg produced reported by Farrell et al. (1981); Bamgbose and Tewe (1994) and Panda et al. (2003).
Neither rice bran levels nor yeast supplementation had any significant effect on the overall hen day egg production. Production figures obtained by birds on all treatment groups were in line with those of Farrell et al. (1981) who reported 57-62% egg production, but lower than the 84.6-86.4% reported by Nahashon et al. (1996). Meeusen (1999) also reported egg production of 90% when he fed high fiber diets supplemented with enzymes. Hen housed production for all treatment diets was low, which was mainly due to mortality.
Egg mass and egg weight did not differ significantly between treatments. The egg mass recorded in this study is within the range of 33-36.4 g/bird/day. The average egg size varied from 56.00-57.00 g. Farrell et al. (1981) and Panda et al. (2003) reported egg weight of 52-57.4 g for layers of 40 to 60 weeks of age. Meeusen (1999) reported higher egg mass of 57.6-58.3 g/bird/day and egg weight of 64.2 g for birds fed high fiber diets supplemented with K-enzyme HF. Onifade et al. (1999) reported egg mass of 31.5-38.52 g/bird/day and an average egg weight of 55-56.43 g when they fed diets containing different levels of maize offal and cassava meal to layers.
Mortality was not influenced by dietary treatments during the laying period. It varied between 4-5% for all treatments, except for treatment 5 where no mortality was recorded. Examination of the dead and sick indicated Mareks disease infection. Mortality recorded in this experiment was lower than the 12% reported by Abdullahi (2004) in his study with Shika brown layers fed a commercial layer diet.
Both the levels of rice bran and yeast supplementation significantly influenced feed cost. The control diet was more expensive than the other diets. High level (35%) of rice bran reduced the cost of feed per kg by about 28% while the lower inclusion rate (30%) reduced the cost by 23%, compared to the control diet. These results indicate that egg production at lower cost could be obtained when rice bran is included in layers diets, with or without yeast addition. Tuleum et al. (1998) observed reduced cost of feed with increasing levels of rice offal in layers diets.
The cost of feed consumed was higher (p<0.05) for the control diet compared to the other treatments, except the high rice bran yeast supplanted diet. This further demonstrates the cost effectiveness of including rice bran in the diets.
Effect of Yeast Supplementation on Egg Quality Characteristics
Albumen height ranged from 5.3 to 5.90 mm across treatments (p>0.05).
The values obtained were slightly lower than those (8.7-9.8 mm) reported by
Apata (1998) and Onifade et al. (1999). Yolk index which varied from
0.32-0.63, was also not influenced significantly by the dietary treatments.
These values fall within the accepted range of 0.33 to 0.50 for fresh eggs (Ihekoronye
and Ngoddy, 1985).
Shell thickness and shell weight were significantly influenced by dietary treatments. Birds on the low rice bran yeast-supplemented diet had thicker shells (0.39 mm) compared to the control (0.29 mm) and those on diets 3 (0.32 mm) and 5 (0.31 mm). These values are lower than those earlier reported by Ayorinde et al. (1999) and Abdullahi (2004) (0.37 to 0.42 mm).
Haugh unit of 87.22-90% recorded for the different dietary treatments in this work were higher than the values (79.57-83%) reported by Ayorinde et al. (1999) and those (56.01-76.82%) reported by Bamgbose and Tewe (1994). The values obtained were however lower than the 93.7-97.87% reported by Apata (1998) and 84-99% reported by Sekoni et al. (1994). The results indicate that irrespective of levels of rice bran and yeast supplementation, high haugh unit could be obtained. According to Kohlmeyer and Shaffner (1944) eggs with haugh unit above 70% are graded A, those with HU of 50-69% are graded B while those with HU below 50% are graded C. All eggs laid in this study thus belong to the A grade, which indicates that neither the levels of rice bran nor yeast supplementation had any negative effect on HU.
Cost of feed was higher for the control group compared to the other treatments,
but the difference was only significant between the control and the diets containing
no yeast. Cost of feed represented 64% of the total cost of production for the
control group. This declined to 63, 62, 60 and 60% for treatments 2, 3, 4 and
5, respectively. In poultry production, cost of feed is said to constitute between
70% (Onifade, 1993; Oluyemi and Roberts, 2000; Agbede and Aletor, 2003) and
88% (Abdullahi, 2004) of the total cost of production. The values obtained in
this study are lower than these estimates, but fall within the range of 50-65%
reported by Tackie and Fleischer (1995). Incorporating the low and high levels
of rice bran into the diets reduced cost of feeding by about 5.8-9.8%.
Revenue was accrued from sale of eggs, spent layers and litter material. Of these, only the revenue obtained through sale of eggs was significantly influenced by the treatments. Birds on the low rice bran yeast supplemented diet (diet 4) generated significantly higher revenue from sale of eggs compared to those on the control and the high rice bran yeast supplemented diets.
The higher revenue generated from birds on the low rice bran yeast supplemented diet was accompanied by a higher margin of N997.83, which was significantly better than that recorded for the control group (N746.95/bird). Similarly, net farm income obtained from birds on low and high rice bran with or without yeast was higher (p<0.05) than that of the control. Reduction in net farm income observed for birds on high rice bran yeast supplemented diet could be related to higher feed intake, which resulted in a concomitant increase in the cost of production.
It could be concluded from the results of this study that feed intake, hen day egg production, feed efficiency and mortality of birds were not affected by the levels of rice bran used, with or without yeast addition.
Among egg quality traits only shell thickness and shell weight were significantly affected by the treatments, with higher values recorded for some of the rice bran containing diets in relation to the control. Economic analysis revealed higher net farm income with the rice bran containing diets compared to the control. It could therefore be concluded that it is more profitable to raise layers on the levels of rice bran used in this study, with or without yeast addition, compared to the type of control diet employed.
1: Abdullahi, A.U., 2004. Performance and egg quality characteristics of the shika brown layer in sokoto, North-West Nigeria. M.Sc. Thesis, Usmanu Danfodiyo University, Sokoto, Nigeria.
2: Abubakar, A., A.A. Sekoni, H.M. Tukur and W.A. Hassan, 2004. Impact of yeast supplementation on the growth performance of pullet chicks fed diets with high levels of rice bran. Proceedings of the 29th Annual Conference of the Nigerian Society for Animal Production, March 21-25, 2004, Usmanu Danfodiyo University, Sokoto, Nigeria, pp: 265-267
3: Adamu, S.B. and C.O. Ubosi, 1998. The influence of growing period, nutrient restriction on laying performance of harco layers. Proceedings of the 3rd Annual Conference of the Animal Science Association of Nigeria, September 22-24, 1998, Lagos Airport Hotel Ikeja, Lagos, pp: 23-25
4: Agbede, J.O. and V.A. Aletor, 2003. Evaluation of fishmeal replaced with leaf protein concentrate from Glyricidia in diets for broiler chicks: Effects on performance, muscle growth, hematology and serum metabolites. Int. J. Poul. Sci., 2: 242-250.
Direct Link |
5: Apata, D.F., 1998. The influence of feeding Prosopis africana seeds on production performance of laying hens. Proceedings of the 3rd Annual Conference of the Animal Science Association of Nigeria, September 22-24, 1998, Lagos Airport Hotel Ikeja, Lagos, pp: 71-73
6: Ayorinde, K.L., J.K. Joseph, O.E. Adewale and I.J. Ayandibu, 1999. Growth, laying and egg quality traits of NAPRI commercial layers on deep litter and Cages. Trop. J. Anim. Sci., 1: 147-155.
7: Bamgbose, A.M. and O.O. Tewe, 1994. Performance and egg quality characteristics of layers fed extruded corn-soybean meal based diets at three energy levels. J. Anim. Prod. Res., 14: 59-67.
8: Ebringerova, A., I. Hromadkova and G. Berth, 1994. Structural and molecular properties of a water-soluble arabinoxylan-protein complex isolated from rye bran. Carbohyd. Res., 264: 97-109.
CrossRef | Direct Link |
9: Farrell, D.J., R. Hamid and R.I. Hutagalung, 1981. Free-choice feeding of laying hens in the humid tropics. Trop. Anim. Prod., 6: 22-29.
Direct Link |
10: Farrell, D.J., 1994. Utilization of rice bran in the diets of domestic fowls and ducklings. World`s Poul. Sci. J., 50: 115-131.
11: Gietema, B., 1992. Chicking Farming: Background Information, Management and Instruction. Gbemi Sodipo Press Ltd., Abeokuta, pp: 197
12: Gunter, K.D., 1989. Yeast Culture`s Success under German Dairy Conditions. In: Biotechnology in the Feed Industry, Lyon, T.P. (Ed.). Technical Publications, Nicholasville. Kentucky, pp: 39-46
13: Ihekoronye, A.I. and P.O. Ngoddy, 1985. Integrated Food Science and Technology for the Tropics. 1st Edn., Macmillan Publishers, London, UK., ISBN: 9780333388839, Pages: 386.
14: Kohlmeyer, E.B. and Shaffner, 1944. Nutritive value of eggs after 21 days storage. Can. J. Agric., 17: 164-168.
15: Kumar, A. and J. Dingle, 1996. Australian experience with yeast culture in broiler diets. Proceedings of the Alltech's 12th Annual Symposium on Biotechnology in the Feed Industry, (BFI'96), Nicholas Ville, KY, USA., pp: 169-171
16: Lyons, T.P., 1987. Yeast culture. A natural feed additive for all species. The Feed Compo-Under, August, pp: 20-23.
17: Martin, S.A., D.J. Nisbet and R.G. Dean, 1989. Influence of a commercial yeast supplement on in vitro ruminant fermentation. Nutr. Rep. Int., 40: 395-403.
18: Meeusen, A., 1999. Optimizing fibre. Asia Pacific Dev., pp: 34-36.
19: Nahashon, S.N., H.S. Nakaue and L.W. Mirosh, 1996. Performance of single comb white leghorn fed a diet supplemented with a live microbial during the growth and egg laying phases. Anim. Feed. Sci. Technol., 57: 25-38.
CrossRef | Direct Link |
20: Odunsi, A.A., O. Sobamiwa and O.G. Longe, 1999. Comparative utilization of alkali-treated and untreated cocoa bean in the diets of egg-type chickens. Trop. J. Anim. Sci., 2: 63-68.
21: Oluyemi, J.A. and F.A. Roberts, 2000. Poultry Production in Warm Wet Climates. 1 Edn., MacMillan Press Ltd., London, pp: 147-168
Direct Link |
22: Onifade, A.A., 1993. Comparative utilization of three dietary fibre sources by broiler chickens. Ph.D. Thesis, University Ibadan, Nigeria.
23: Onifade, A.A. and G.M. Babatunde, 1996. Supplemental value of dried yeast in a high fiber diet for broiler chicks. Anim. Feed Sci. Technol., 62: 91-96.
Direct Link |
24: Onifade, A.A., O.O. Tewa, A.O. Fanimo, O.O. Okunola and A.B. Bafolabi, 1999. Replacement value of cashew nut meal for ground nut cake in pullet diets: Effect on pre-laying performance and serum biochemical indices. Indian J. Anim. Sci., 68: 273-275.
Direct Link |
25: Onifade, A.A., A.A. Odunsi, O.A. Adebisi, A. Abubakar and A.E. Enohoebot et al., 2000. Comparison of the performance of starting pullets fed supplemental bakers or feed-grade yeast in diets containing high levels of palm kernel meal. Arch. Gefluigelk, 64: 264-268.
Direct Link |
26: Panda, A.K., M.R. Reddy, S.V. Rama Rao and N.K. Praharaj, 2003. Production performance, serum/yolk cholesterol and immune competence of white leghorn layers as influenced by dietary supplementation with probiotic. Trop. Anim. Health Prod., 35: 85-94.
CrossRef | Direct Link |
27: Rose, A.H., 1987. Yeast, a Microorganism for All Species. In: A Theoretical Look at its Mode of Action. Biotechnology in the Feed Industry, Lyons, T.P. and K.A. Jacques (Eds.). Alltech's Technical Publishers, USA
28: SAS., 1990. Statistical Analysis for Social Sciences: User's Guide. SAS Institute, Inc., Cary, NC, USA
29: Sekoni, A.A., A.O Aduku, S.O. Ogundipe and J.O. Bale, 1994. The effects of graded levels of cassava peel meal on laying hens. J. Anim. Prod. Res., 14: 32-36.
30: Steel, R.G.D. and J.H. Torrie, 1984. Principles and Procedures of Statistics. 2nd Edn., McGraw Hill Book Co. Inc., New York, pp: 172-177
31: Tackie, A.M. and J.E. Fleisher, 1995. Nutritive value of wild sorghum fortified with Leucaena leucocephala (Wh, Lam). Bull. Anim. Health Prod. Africa, 43: 223-275.
32: Tuleum, C.D., P.C. Njoku and I.D.I. Yaakugh, 1998. The performance of pullet chicks fed roxaxyme in diets containing graded levels of rice offal. Proceedings of the 29th Annual Conference of the Nigerian Society for Animal Production, March 21-25, 1998, Usmanu Danfodiyo University, Sokoto, pp: 74-76
33: Wallace, R.J., 1997. The mode of action of yeast culture in modifying rumen fermentation. In: Proceedings of Alltech`s 12th Annual Symposium, Lyons, T.P. and K.J. Jacques (Eds.). Alltech International Publishers, Nicholasvile KY, USA
34: Wang, G.J., 1997. Effects of enzyme supplementation and irradiation of rice bran on the performance of Leghorn broiler chicks. pp: 1-10. http:/www.idrc Ca/books/focus/821/chp.7. Html.
35: Warren, B.E. and D.J. Farrell, 1990. The nutritive value of full-fat and defatted Australian rice bran. IV. Egg production of hens on diets with defatted rice bran. Anim. Feed Sci. Technol., 27: 259-268.
CrossRef | Direct Link |
36: Warren, B.E. and D.J. Farrell, 1991. The nutritive value of full-fat and defatted Australian rice bran. V. The apparent retention of minerals and apparent digestibility of amino acids in chickens and adult cockerels fitted with ileal cannulae. Anim. Feed Sci. Technol., 34: 323-342.
CrossRef | Direct Link |