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Articles
by
Zainul Amiruddin Zakaria |
Total Records (
1 ) for
Zainul Amiruddin Zakaria |
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Nor Asmara Tasrip
,
Mohd Nasir Mohd Desa
,
Cheah Yoke Kqueen
,
Nurul Zarith Mohamad Zin
,
Zainul Amiruddin Zakaria
,
Zarizal Suhaili
,
Chong Pei Pei
and
Mariana Nor Shamsudin
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This study compared the distribution of aerial mass colors,
antibacterial activities and RAPD-based genomic patterns of 39 Streptomyces
isolates harvested mainly from soil at the University Agricultural Park, Universiti
Putra Malaysia. Streptomyces griseus ATCC 10137 strain was also included
as a reference strain (n = 40). Based on ISP-2 media, the aerial mass colors
observed were categorized as yellow (n = 15), grey (n = 9), brown (n = 7), white
(n = 6) and others (n = 3). Antibacterial activities were assessed on Mueller
Hinton Agar (MHA) and Tryptic Soy Agar (TSA) by perpendicular streak method
against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC
25922, Salmonella sp. and Enterococcus sp. MHA demonstrated 15
isolates with broad spectrum antibacterial activities and 18 as non-broad spectrum.
TSA gave lower proportions with 15 and 9 isolates respectively. Regardless of
the test media used, a higher proportion of isolates with non-white color showed
antibacterial activities to suggest a potential correlation. RAPD dendrogram
(a composite of 3 random primers) also clustered majority of them but segregated
those of white color, which showed less antibacterial activities, in a different
cluster. Further validations involving more isolates are warranted to establish
the findings. |
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