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Articles by Yuemao Shen
Total Records ( 4 ) for Yuemao Shen
  Thongchai Taechowisan , Nantiya Chuaychot , Srisakul Chanaphat , Asawin Wanbanjob and Yuemao Shen
  Some endophytic actinomycetes (120) were isolated from the roots of Alpinia galanga. Identification of these endophytes was based on their morphology and amino acid composition of the whole-cell extract. Most isolates were classified as Streptomyces sp. (82), with the remainder belonging to Nocardia sp. (11), Microbispora sp. (3) and Micromonospora sp. (2). Eight isolates were unclassified and 14 were lost during subculture. The strain identified as endophytic Streptomyces sp. Tc052 strongly inhibited test microorganisms. This endophyte was cultured, the agar was extracted with organic solvent and the extract was purified on a column of silica gel to give a major component, which was identified to be kaempferol, isoscutellarin, umbelliferone and cichoriin on the basis of spectroscopic data. These compounds together with the extract were tested for their antimicrobial activity against bacteria and yeast using micro-dilution methods for the determination of Minimum Inhibitory Concentrations (MIC) and Minimum Microbicidal Concentration (MMC). The MIC values obtained with the crude extract varied from 64-128 μg mL-1 against tested microorganisms. All the isolated compounds showed various activities.
  Yaoyao Li , Chunhua Lu , Zhiyu Hu , Yaojian Huang and Yuemao Shen
  One new diterpene glycoside (1) named 16-O-α-D-tetraacetylglucopyranosyl hymatoxin C, along with eight known ones including two diterpenoids (2, 3), three cytochalasins (4, 5, 6), dihydroisocoumarin (7) and two C7-tetraketides (8, 9), were isolated from the culture of the endophytic fungal strain Tubercularia sp. TF5. The structures of compounds 1-9 were elucidated by spectroscopic methods. The cytotoxic and antimicrobial activities of 1 were analysed but showed no significant activities.
  Ting Lin , Chunhua Lu and Yuemao Shen
  Twelve compounds that belonged to five structure types, namely saturated dibenzylbutyrolactone lignans (1-3), sesterterpenoids (4-6), anthraquinone (questin, 7), benzophenone (dihydrogeodin, 8) and diphenyl ethers (9-12), were isolated from the extract of the strain Aspergillus sp. F1, which was isolated from the seeds of Trewia nudiflora. Among them, compounds 1, 11 and 12 were determined to be new, namely butylrolactone-V, butyl 2,4-dichloroasterrate and methyl 2,4-dichloroasterrate, respectively. The structures of these compounds were characterised on the basis of spectroscopic data.
  Thongchai Taechowisan , Asawin Wanbanjob , Pittaya Tuntiwachwuttikul , Yuemao Shen and Saisamorn Lumyong
  Different extracts of Streptomyces aureofaciens CMUAc130 culture were studied as potential antifungal agents for selected phytopathogenic fungi. In a serial agar dilution method, crude ethyl acetate and 10% methanol in ethyl acetate extracts exhibited fungistatic activity against Aspergillus flavus, Colletotrichum musae, Fusarium oxysporum, Pythium ultimum, Rhizoctonia solani and Sclerotium rolfsii. Both ethyl acetate extract and 10% methanol in ethyl acetate extract were highly effective on all tested fungi, with Minimum Inhibitory Concentration (MIC) values ranging from 0.25 to 50 and 10 to 100 mg mL-1, respectively. The major active ingredients from those extracts were purified by silica gel column chromatography and identified to be 5, 7, 4`-trimethoxy-4-phenylcoumarin (1), 4`-hydroxy-5,7-dimethoxy-4-phenylcoumarin (2), 3`-Hydroxy-5,7,4`-trimethoxy-4-phenylcoumarin (3), 5,7,3`,4`-Tetramethoxy-4-phenylcoumarin (4) and 4`-hydroxy-5,7,3`-trimethoxy-4-phenylcoumarin (5) by NMR and mass spectral data, respectively. Five compounds (1 to 5) had activity against F. oxysporum with MICs of 0.30, 1.00, 0.40, 10.00 and 20.00 mg mL-1, respectively. Compounds 1, 2 and 3 also showed a synergistic effect when combined in different concentrations, displaying four times less concentration to reach complete inhibition in the growth of F. oxysporum.
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