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Articles by Yuanfang Gong
Total Records ( 4 ) for Yuanfang Gong
  Shaoqian Cai , Yang Liu , Chenhua Zhang , Weixuan Fu , Yuanfang Gong , Xin Lu , Qin Zhang and Zongjun Yin
  This study was aimed to identify Quantitative Trait Loci (QTL) for haematological traits in pig chromosome 10 and facilitate the cloning of candidate genes underlying the QTLs. Hematological traits are essential parameters for evaluating the health status of animals and play a extremely important role in disease resistance. In this study, three main components, leukocyte traits, erythrocyte traits and platelet traits were measured in a composite pig population consisting of 445 pigs of three breeds (Landrace, Large White and Songliao Black Pig) distributed in 16 boar families, before and after vaccination with modified live CSF (classical swine fever) vaccine. A partial genome scan for mapping Quantitative Trait Loci (QTL) for these traits was performed by genotyping 13 microsatellite markers on chromosome 10. Through a linear mixed model and the permutation for empirical threshold values, 4 significant QTLs on chromosome 10 were identified affecting hematocrit (HCT), hemoglobin (HGB), Mean Corpuscular Volume (MCV) and blood platelet counts (PLT) (p<0.05), respectively. Our results confirms that haematological traits variation differs between the three pig breeds and variations of HCT, HGB, MCV, PLT are associated with the 81~133 cM region in chromosomal 10.
  Yang Liu , Xiaotian Qiu , Jingjing Xu , Fang Hu , Yuhua Li , Hejun Li , Yuanfang Gong and Qin Zhang
  Solute carrier family 11 member 1 (SLC11A1) plays an important role in the innate immune response against intracellular pathogens. To evaluate effect of SLC11A1 gene on immune response capacity in pigs, one SNP in intron1 was demonstrated by PCR-RFLP and sequencing method. Immune response traits include six normal hematology traits (white blood cell, red blood cell, hemoglobin, granulocyte, lymphocyte and monocyte), four T lymphocyte subpopulations (CD4¯CD8¯, CD4¯CD8+, CD4+CD8¯, CD4+CD8+) and CSFV antibody titer were measured in three pig breeds (Large White, Landrace and Songliao Black) after vaccinated with classical swine fever live vaccine at the 21st day after birth. The further association analysis between the SNP genotype and immune response traits were conducted. The SNP of SLC11A1 gene had significant effect on level of monocyte (p = 0.010) and CD4¯CD8+ percentage (p = 0.041). The animals with AA genotype had significant higher monocyte and CD4¯CD8+ percentage than that of animals with GG and GA genotype (p<0.05). Present results also suggest that SLC11A1 could be a marker gene for genetic selection of disease susceptibility in pigs.
  Zhengzhu Liu , Yuanfang Gong , Wenjin Zhu , Lingxin Duan , Muxiang Ge , Baochang Shi , Minshan Feng and He Gao
  In order to explore the sequence structure of fox Agouti gene and it’s mechanism to regulate the pelage color’s dividing. In this study, the major part of intron 2 sequence (1038 bp) of Agouti gene from the silver fox were obtained by PCR amplification and direct sequencing. This sequence was aligned with red fox, giant panda, horse, pig, goat, cattle, sheep, domestic cat and rabbit and the sequence similarities were 100, 85.82, 75.75, 73.31, 66.22, 65.98, 65.77, 60.45 and 58.82%, respectively. The result of the homology analysis showed that the genetic relationship between silver fox and red fox was the highest, which was consistent with that they belong to Vulpes of the Canidae animal in traditional classification. Based on the sequence of Agouti gene intron 2, the phylogenetic tree was constructed for silver fox and the other 9 species using Clustalx (1.83) software. The cluster result of phylogenetic tree of all species was basically consistent with the taxonomy of NCBI and was similar to the physiological characteristics of the species and their traditional classification. The above results provide the important biological information for researching the mechanism of the formation mechanism of the coat color and artificially improving the coat color quality of fox and so on.
  Zhengzhu Liu , Yuanfang Gong , Lingxin Duan , Minshan Feng , Wenjin Zhu , Muxiang Ge , Xierong Liu , Junsheng Zhao and Xinling Guo
  To explore the sequence structure of fox TYR gene and it’s mechanism to regulate the pelage color’s dividing. In this study, the partial DNA sequence (1370 bp) of silver fox TYR gene was obtained by PCR amplification and direct sequencing. This sequence contained the intron 1 of 368 bp, the complete exon 2 of 217 bp and the intron 2 of 785 bp and GC content was 40.5%. Based on the sequences of TYR gene exon 2, the homological analysis and the construction of un-rooted phylogenetic tree were carried out among silver fox and the other 16 species by using DNAMAN software and Clustalx (1.83) software, respectively. The homological analysis showed that silver fox had the closest genetic relationship with dog and the closer genetic relationship with domestic cat and domestic ferret. The cluster result of un-rooted phylogenetic tree was similar to that of the homological analysis. The length of TYR gene exon 2 were 217 bp for all analyzed species, which indicated that TYR gene exon 2 were highly conservative in phylogeny. The above results can provide the important biological information for further researching the development and regulation mechanism of fox coat color which result from TYR gene.
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