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Articles by Yousef Doustar
Total Records ( 8 ) for Yousef Doustar
  Daryoush Mohajeri , Ghafour Mousavi , Mehran Mesgari , Yousef Doustar and Mir Hadi Khayat Nouri
  The medicinal properties attributed to Crocus sativus L. (saffron) are extensive. The safety of saffron is important in relation to its medicinal applications. This study was performed to elucidate the possible toxic effects of ethanolic extract of Crocus sativus L. stigma on liver, kidney and some hematological parameters in rats. Wistar rats were randomly assigned into four groups of eight animals each. Group 1 was treated with ISS as control and Groups 2 to 4 were treated with extract administered daily for 2 weeks intraperitoneally in doses of 0.35, 0.70 and 1.05 g kg-1, respectively. Body weight of the animals were recorded on the first, seven and final days of the experiment. The haematological studies include total RBC count, total WBC count, Hb, %HCT, MCV, MCH and MCHC. Biochemical/serum profile studies include ALT, AST, urea, uric acid and creatinine. Tissue specimens of the liver and kidneys were subjected to histological examination using standard hematoxyline-eosin staining. The extract caused significant reductions in the Hb and HCT levels and total RBC count, although it showed any dose-dependent effect. Total WBC count showed significant dose-dependent increases in extract treated rats. Significant dose-dependent increased values of AST, ALT, urea, uric acid and creatinine were seen. Microscopically, there were mild to severe hepatic and renal tissue injuries supporting the biochemical analysis. The results indicated that extract of Crocus sativus L. stigma is toxic in high doses.
  Yousef Doustar , Daryoush Mohajeri , Younes Anzabi and Mehrdad Nazeri
  Influenza virus produces cell death in animals and human. Since cell death can be caused by either necrosis or apoptosis. We investigated the types of cell death that occur in chickens infected with avian influenza virus, A/chicken/Iran/772/2000(H9N2). In experimental study 60 SPF chickens at 3 weeks old were divided to two groups. The first group was infected with 107.5 EID50 titer of the virus intranasaly and the second group was treated with saline normal. Following 72 hrs, renal tissues were collected and fixed in 10% formalin solution. The prepared microscopic sections with the thickness of 5-6 micron were stained using TUNEL method. In comparison to the control group, there were significant mean difference of apoptotic cells in renal tubular cells of the infected group (p<0.005). We demonstrated that A/chicken/Iran/772/2000 (H9N2) is able to induce apoptosis in renal tubular cells.
  Yousef Doustar , Daryoush Mohajeri , Alireza Garjani , Ghafour Mousavi and Mehrdad Neshat Ghramaleki
  The heart failures following infarctions is one of the most important causes of death throughout the world. This study was undertaken to investigate the protective effects of metformin on apoptotic cell death of cardiomyocytes during experimental cardiac Ischemia-Reperfusion (IR) in rats. The 25 male Wistar rats were randomly assigned into 5 groups of 5 animals each including; Sham/IR, IR, low dose metformin+IR, average dose metformin+IR and high dose metformin+IR. Heart muscle ischemia was induced clamping the left descending coronary artery. After 30 min of ischemia, the clamps were taken off and the animals underwent 2 h reperfusion. Metformin (5, 10 and 20 μg/kg/min) was infused 15 min prior to reperfusion through jugular vein in treatment groups. At the end of experiment, the rats were euthanized and histological sections from left ventricles were prepared through Tunnel Staining method. Apoptotic cells were counted under light microscope. The data obtained were statistically analyzed using ANOVA. Differences were considered statistically significant at p<0.05. In group 2, ischemia-reperfusion caused occurrence of apoptotic cell death in cardiomyocytes. There was a significant increase in the incidence rate of apoptosis of cardiomyocytes in comparison with group 1 (p<0.001). In groups 3-5 metformin (5, 10 and 20 μg/kg/min) caused significant decrease in the number of apoptotic cells in comparison with group 2 (p<0.05, p<0.01 and p<0.001, respectively). This study therefore, suggests that metformin may be a useful agent for the prevention of Ischemia-Reperfusion (IR) induced apoptotic cell death of cardiomyocytes in a dose dependent manner in the rats.
  Ali Akbar Abolfathi , Yousef Doustar , Pejman Mortazavi and Ali Rezai
  Ischemia/Reperfusion (IR) induces severe tissue injury mainly caused by oxidative stress. A considerable body of recent evidence suggests that oxidative stress and exaggerated production of reactive oxidant species play a major role in several aspects ischemia and reperfusion. Hypericum perforatum is a medicinal plant species containing many polyphenolic compounds namely flavonoids and phenolic acids. Because polyphenolic compounds have high antioxidant potential in this study we evaluated the effect of H. perforatum extract on renal ischemic reperfusion injury. Renal ischemic reperfusion was induced in rats by clamping the renal artery for 60 min. After 7 days of reperfusion. H. perforatum extract treatment markedly reduced renal ischemia reperfusion injury in I/R+HP 30 mg kg-1 group comparative with other treatment and sham groups.
  Mehrdad Hashemi , Yousef Doustar , Fataneh Hashem Dabaghian and Seyed Ashrafadin Goushegir
  Grape Seed Extract (GSE) has been reported to exert protective effects on various forms of renal ischemic reperfusion disorders. Natural Grape Seed Extract (GSE) are potent free radical scavengers and hence, provide significant protection against oxidative stress. Accordingly, the present study focused on investigating the possible protective role of GSE against ischemic reperfusion mediated damage in renal tissues in rats. The results revealed that oral administration of 250 mg kg-1 (body weight) of GSE for 3 days significantly protect against renal cell apoptosis via the induction of endogenous antioxidant (p<0.001).
  Yousef Doustar and Alireza Garjani
  Colorectal cancer is a major health problem worldwide. Resveratrol is a stilbenoid, a type of natural phenol and a phytoalexin produced naturally by several plants when under attack by pathogens such as bacteria or fungi. The main objective of present study was to immunohistochemical assessment of the effect of resveratrol on the expression of β-catenin protein in experimental colonic carcinoma of rat. The 25 male Wistar rats aged 3-4 months old weighting 250-350 g were selected by chance. The rats of group 1 were received standard food and water without any changes in their nutritional condition. Rats of group 2 were received EDTA at the dose of 40 mg kg-1 as promotor of DMH twice a week for 2 weeks. Rats of group 3 were received DMH at the dose of 40 mg kg-1 twice a week for 2 weeks for induction the cancer. The rats of groups 4 and 5 after induction of cancer were received resveratrol at the dose of 10 and 20 mg/kg/day for 10 weeks orally, respectively. The 12 weeks after treatment with resveratrol, animals were constrained and anesthetized by xylazine and ketamine intraperitoneally. Then, segments of colon were sampled for histopathological assessments. Immunohistochemical evaluations showed that rate of the expression of β-catenin proteins in treatment groups was less than control group and there is a statistical significance among groups (p<0.01).
  Daryoush Mohajeri , Ghafour Mousavi and Yousef Doustar
  Adequate characterization of hypoglycemic effect of ethanolic saffron extract has not been yet done, though the activity has been reported. The scientific evaluation of its hypoglycemic activity was, therefore, explored and compared with the effect of a standard hypoglycemic drug, tolbutamide. In this study, we also report on alteration in patterns of pancreatic islet cells using histopathology and immunohistochemistry of alloxanized diabetic rats treated with ethanolic saffron extract. The ethanolic extract of Crocus sativus L. stigma was administered orally and intraperitoneally at different doses (20, 40 and 80 mg kg-1) to normal rats for finding the more effective hypoglycemic dose and administration route. Acute hypoglycemic effects produced by more effective dose of ethanolic saffron extract on the Fasting Blood Glucose (FBG) levels and effects of the same dose of ethanolic saffron extract on the FBG and plasma insulin levels in alloxanized Mild Diabetic (MD) and Severely Diabetic (SD) rats were assayed. Histopathological and immunohistochemical studies were also carried out on pancreatic islet cells of control and diabetic rats. The dose of 40 mg kg-1 was found to be more effective dose in intraperitoneally (i.p.) route for decreasing Blood Glucose Level (BGL). The extract administered by i.p. route at more effective dose showed an acute hypoglycemic effect in MD and SD rats. Treatment of MD and SD rats for 14 days with the more effective dose significantly reduced the FBG levels in these animals (41.4% MD, 30.7% SD). Serum insulin level showed significant increase in diabetic rats (33.3% MD, 27.3% SD) after 14 days. The histopathological studies of pancreas in ethanolic extract treated diabetic groups showed a reversed damage caused by alloxan to the pancreatic islets as almost normal appearance. In addition, diabetic (MD and SD) rats showed obvious decreases in insulin immunoreactivity and the number of β-cells in pancreas, but the pancreas of extract-treated diabetic rats was improved and the number of immunoreactive β-cells was significantly increased. The control group given saffron extract was not different from the other intact control group considering the insulin immunoreactivity in β-cells. The findings of present study indicate the hypoglycemic and potential antihyperglycemic nature of the extract, helping in regeneration of damaged pancreas in experimental diabetes. Thus, after randomized clinical trials, saffron extract may be implicated as a preventive or therapeutic agent against diabetes mellitus.
  Yousef Doustar , Younes Anzabi , Amirreza Ebadi and Behbood Jafari
  Influenza is a viral disease which has been known in 1901AD in 1955, a kind of influenza virus was known as a factor caused disease that later on called avian plague because of high rate mortality. In the present study, the sub-type H9N2 of avian influenza virus (obtained from Razi Serum Producing and Research Center) which was cloned two times in embryonic eggs was inoculated to the SPF (Valo, Lohman, Germany) at their 3rd week by nasal drops: At first, SPF chickens were divided to 2 groups of 10 subjects; a group as a treatment group and another one as a control group. Then treatment group was infected by H9N2 sub-type of influenza virus with dosage of 107.5 EID50. The control group obtained normal saline serum nasally equal to the inoculated viral solution volume. In histopathological studies of lung and pleura of treated chicks with H9N2 sub-type influenza virus, propagated pneumonia which accompanies hyperemia and severs hemorrhage was observed in to allele and pleurisy sacs. In microscopic view, there is sever hyperemia and hemorrhage in allelic sacs, edama infiltration of fibrin and inflammatory cells, especially heterophylus in pulmonary' tissue inter-space. In the present study, the apoptosis of primary bronchial cells was observable in some cases which demonstrate significant changes compared with control group. Probably, the expression of apoptosis induction channel which has been discussed in Brydon's studies is a suitable justification for these changes. So, it must be noted that there are various channels in occurrence of cell death following infection by influenza virus and knowing these channels needs extensive studies.
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