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Articles by Yongjiang Luo
Total Records ( 2 ) for Yongjiang Luo
  Hui Wang , Xia Zhang , Pengcheng Dong , Yongjiang Luo and Fusheng Cheng
  Microbial polysaccharides are located both inside the fungal cell walls and on the fungal cell surfaces and possess marked immunological properties. In this study, the extraction conditions of Saccharomyces cerevisiae polysaccharides (SCPS) were optimized using response surface methodology and the immune enhancement properties of SCPS were evaluated. The results indicated that the optimal extraction conditions were high pressure homogenization time of 35 min, ultrasonic power of 510 W and ultrasonic time of 26 min, respectively. Under these conditions, the maximal observed value extraction yield of S. cerevisiae polysaccharides (SCPS) was (29.84±0.09)%, which was agreed with predicted value 29.82%. Thin layer chromatography (TLC) exhibited SCPS may be a glucan. Pharmacological experiments indicated that SCPS could increase alkaline phosphatase (AKP), lysozyme (LZM), immunoglobulin A (IgA), immunoglobulin G (IgG) and Epidermal Growth Factor (EGF) levels in serum, increase secreted immunoglobulin A (SIgA) expression in jejunum secretion and decrease prostaglandin E2 (PGE2) expression in colon of immune-compromised rats at medium-dose. Saccharomyces cerevisiae polysaccharides has significant immune enhancement activity and could obviously protect intestine mucosa of immune-compromised rats.
  Ling Wang , Feng Yang , Xiaojuan Wei , Yongjiang Luo , Xuzhen Zhou , Wenzhu Guo , Jiangrong Niu and Zhiting Guo
  The objective of this study was to determine the prevalence of mastitis, identify the frequency alteration of predominant pathogens related to clinical and subclinical mastitis in Gansu and Ningxia Province of China. The 29 dairy farms comprising 2000 lactating cows enrolled from April 2012 to November 2014 of which 43.0% (860/2000) were found positive for mastitis by physical examination and Lanzhou Mastitis Test (LMT), the prevalence of Clinical Mastitis (CM) and Subclinical Mastitis (SCM) at cow level were 5.10 and 37.90%, respectively. The proportion of samples contained major pathogens were 97.60% and 871 isolates obtained from 860 milk samples. The common isolates from CM were E. coli (26.89%), S. agalactiae (21.01%) and S. aureus (19.33%) while from SCM were S. agalactiae, S. dysgalactiae and S. aureu, accounted for 30.45, 18.62 and 14.89%, respectively. The contagious pathogens had showed to predominate over the environmental pathogens and the Streptococcus sp. was found the predominant species which remains a significant cause of mastitis in local cattle herds.
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