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Articles by Yamssi Cedric
Total Records ( 2 ) for Yamssi Cedric
  Emmanuel Tana Toah , Yamssi Cedric , Noumedem Anangmo Christelle Nadia , Vemo Bertin Narcisse , Sop Foka Eric Igor , Atiokeng Tatang Rostand Joël and Vincent Khan Payne
  Background and Objective: Coccidiosis is caused by a protozoan parasite of the genus Eimeria which affects poultry industries worldwide. This study was conducted to comprehend the impact of C. aegyptiaca ethanolic extracts on chicken coccidiosis. Materials and Methods: Six groups of chicks in a complete randomized design were used. Each chick among five groups was orally challenged with 2×104 Eimeria tenella sporulated oocysts. The first three groups received the decoction of C. aegyptiaca orally at 400, 200 and 100 mg kg–1, respectively, while the fourth group received anticox. The fifth group was the negative control. Faecal oocyst counts, zoo-technical parameters, serum levels of aminotransferases, creatinine, uric acid, bilirubin and proteins were evaluated. Antioxidant parameters such as Catalase, superoxide dismutase, peroxidase and the level of malondialdehyde in serum were also assessed. Analysis of Variance was performed in one way to analyse data at a 5% level of probability. Results: Oocyst counts showed a significant (p<0.05) reduction rate in all the medicated groups. Zoo-technical parameters, total proteins and albumin contents were significantly higher in the medicated groups. Also, serum levels of ALAT, Creatinine, uric acid, total and direct bilirubin were significantly lower in the medicated chickens. SOD and peroxidases presented significantly lower activities and lower contents of malondialdehyde in the medicated groups than the negative control group in each case. Conclusion: Results from this study could be used for developing phyto elements that can serve as an alternative to synthetic anticoccidial and antioxidant drugs.
  Yamssi Cedric , Vincent Khan Payne , Noumedem Anangmo Christelle Nadia , Norbert Kodjio , Etung Kollins , Leonelle Megwi , Jules-Roger Kuiate and Mpoame Mbida
  Background and Objective: Coccidiosis remains one of the most important infectious cause of digestive disorders in rabbits. The aim of this study was to evaluate in vitro anticoccidial and antioxidant activities of Psidium guajava (P. guajava) extracts. Materials and Methods: Sporulation inhibition bioassay was used to evaluate the activity of Psidium guajava extracts on sporulation of Eimeria flavescens, Eimeria stiedae, Eimeria intestinalis and Eimeria magna oocysts and sporozoites. The set up was examined after 24 and 48 h for the oocysticidal activities and after 12 and 24 h for anti-sporozoidal activities. The antioxidant activity was determined by measuring FRAP (ferric reducing-antioxidant power), 1,1-Diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging and nitric oxide (NO) radical scavenging. The cytotoxicity of the most active extract was determined against animal cell lines fibroblast L929, HEPG2 and HeLa cells using MTT assay. The impact of the toxicity was established by analyzing the selectivity index (SI) values. Data obtained were analyzed using one-way analysis of variance (ANOVA) and were determined by Waller-Duncan test using SPSS. Results: The highest efficacy of tested plant extracts was recorded after 24 h, which varied according to different concentrations of the tested extracts. The highest efficacy was 88.67±2.52% at the concentration of 30 mg mL–1 of the methanolic extract against E. intestinalis. Most extracts including the aqueous extract exhibited good anti-sporozoidal activities against E. flavescens, E. stiedae, E. intestinalis and E. magna sporozoites at 1000 μg mL–1. The highest viability inhibitory percentage was 97.00±1.73% at a concentration of 1000 μg mL–1 of P. guajava methanolic extract against E. intestinalis sporozoites. These results also showed that methanolic and ethyl acetate extract, possessed strong antioxidant activities (IC50<20 μg mL–1). The methanolic extract of P. guajava exhibited CC50 of >30 μg mL–1 against selected cell lines, suggesting that the compounds were not toxic. Phytochemical screening of the most active extract showed presence of alkaloids, flavonoids, saponins and phenols. Conclusion: These results provide confirmation to the usage of Psidium guajava against
coccidiosis by Agricultural farmers in Cameroon.
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