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Articles by Y.W. Ho
Total Records ( 3 ) for Y.W. Ho
  R. Kalavathy , N. Abdullah , S. Jalaludin , C.M.V.L. Wong and Y.W. Ho
  A feeding experiment was carried out for 42 days to evaluate the effects of a mixture of 12 Lactobacillus cultures (LC) or oxytetracycline (OTC) on growth performance and serum lipids of broiler chicks (Hubbard). Two hundred and seventy 1-day-old chicks were assigned randomly to three dietary treatments. The treatments were (i) a basal diet (control) (ii) basal diet+1 g kg-1 LC and (iii) basal diet+50 mg kg-1 OTC. Each dietary treatment had six replicate cages with 15 chicks per cage. Body weights and feed to gain ratios of broilers were determined at 21 and 42 days of age while serum lipids were determined at 42 days of age. From 1 to 42 days of age, broilers fed LC or OTC had significantly (p< 0.05) better growth than the control broilers. The feed conversion ratios were improved significantly (p< 0.05) during the growing (1 to 21 days of age) and finishing (22 to 42 days of age) periods in broilers fed LC or OTC, but the best feed to gain ratio was obtained in the LC-fed broilers. Serum total cholesterol was significantly (p< 0.05) reduced in broilers supplemented with LC as compared to broilers receiving OTC or control diet. Low density lipoprotein cholesterol and triglycerides were significantly (p< 0.05) lower in broilers fed LC but significantly (p< 0.05) higher in broilers fed OTC when compared to the control broilers. The results indicated that LC had a hypocholesterolaemic effect on broilers in contrast to OTC.
  C.C. Sieo , B.T. Wong , N. Abdullah and Y.W. Ho
  Whole-cell protein fingerprinting has been used in various studies for identification, characterization and differentiation of bacteria. It has been observed that the methods of extraction of the whole-cell protein and age of the cells used were varied in several studies. Thus, in this study, the effects of different extraction methods and age of cells on the whole-cell protein patterns were evaluated. The whole-cell proteins from Lactobacillus reuteri C1, C10 and C16, Lactobacillus salivarius I24 and Lactobacillus gallinarum I16 and I26 were extracted from 6, 12, 18 and 24-h-old cells by using lysozyme treatment, glass beads and sonication. A comparison of the whole-cell protein profiles generated showed that extraction by lysozyme treatment produced the most complete profile, with protein bands at high intensities. Glass beads and sonication could only extract major proteins. The age of cells also affected the whole-cell protein profile. Different strains were found to produce optimum results at different cell ages when extracted with the three methods.
  S.W. Zuhainis , N. Abdullah , A.R. Alimon and Y.W. Ho
  Anaerobic fungi were isolated from the rumens of cattle, buffalo and goat. A total of 133 isolates were obtained. Three of these isolates namely Neocallimastix frontalis B9, Piromyces mae B6 and Orpinomyces joyonii C3 were studied for their cellulolytic activity by using 14C-cellulose produced by Acetobacter xylinum as the substrate. The effects of phenolic acids on the cellulolytic activity of a fungal isolate N. frontalis B9 from buffalo was also determined. The results showed that among the fungal species, N. frontalis B9 had the highest cellulolytic activity, followed by P. mae B6 and O. joyonii C3. The cellulolytic activities of N. frontalis isolates from buffalo, cattle and goat were not significantly different. Both ρ-coumaric and ferulic acids reduced significantly (p<0.05) the celluloytic activity of N. frontalis B9.
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