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Articles by Xiao-Rong Zhang
Total Records ( 2 ) for Xiao-Rong Zhang
  Zi-Jun Zhang , Ying-Hui Ling , Chun-Huan Ren , Xiao Cheng , Hong-Guo Cao , Xiao-Fei Guo , Ya-Feng Huang and Xiao-Rong Zhang
  Myostatin (MSTN) was a major gene of skeletal muscle growth regulation and a member of the transforming growth factor-β (TGF-β) super family. It acts as a negative regulator of skeletal muscle growth. To knock out goat MSTN gene of nuclear donor cells correctly, a gene targeting vector should be constructed. The homologous arm of goat MSTN was amplified by PCR and restriction enzyme digested. Intermediate vector pMD18-SA and pMD18-LA were constructed by conventional molecular cloning method. The final target vector pLOXP-MSTN was constructed by step-by-step cloning. The replacement type knock-out vector was transfected into goat fetal fibroblast by electrotransfection. The homologous arm sequences of goat MSTN including the 4.6 kb homologous long arm (LA) and the 1.9 kb homologous Short Arm (SA) were successfully amplified using LA-PCR technology. The LA and SA were inserted into vector pLOXP, generating MSTN gene targeting vector pLOXP-MSTN which contained neo and tk positive-negative-selection markers. The pLOXP-MSTN was linearized and electroporated into the nuclear donor cells. After G418 and GANC selection, fifty-eight drug resistant cell clones were screened and from which totally 3 positive clones were confirmed by PCR and DNA sequencing. The constructed gene targeting vector could efficiently target of MSTN locus and the gene knockout cell clones will be used to produce MSTN knockout goat by means of somatic cell nuclear transplantation.
  Yinghui Ling , Lijuan Wang , Xiaodong Zhang , Lina Xu , Jian-Ping Ding , Yun-Hai Zhang , Zijun Zhang and Xiao-Rong Zhang
  This study is about the correlation between COX II gene genetic variation and adult buck sperm motility traits, to provide genetics reference for the goat breeding. In this study, 120 adult male goat semen samples were collected from four populations. COX II gene polymorphism information was rapid screened using DNA pool and sequencing method. Each individual polymorphism of these goats was detected by RFLP. Sequencing results of DNA pool showed a single nucleotide mutation in COX II gene (A→G). The mutation caused a change of Hind III restriction site. RFLP results showed that the mutation associated with goat sperm traits. The vitality of fresh sperm and frozen sperm from BB-type goats was significantly higher than AA-type. This study provided foundation for establishment relationship between COX II gene SNP and goat sperm motility traits. It could be considered as a reference marker-assisted selection for buck semen quality traits and goat breeding.
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