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Articles by Wu Tian-Wen
Total Records ( 3 ) for Wu Tian-Wen
  Wu Tian-Wen , Xu Gui-Jiang , Pan Yu-Lai , Xie Xi-Ping , Li Bi-Chun and Wu Xin-Sheng
  The genetic diversity and phylogenetic relationship of 7 China rabbit populations were investigated with 15 microsatellite. The results showed that: The value of the average expected heterozygosity (He range from 0.820±0.012-0.675±0.031) Polymorphic Information Content (PIC range from 0.796±0.016-0.625±0.033) and the mean effective number of alleles (Ne = 6.625±0.498) of the seven rabbit populations were high, which indicated that polymorphisms and genetic diversity of genes were abundant. The range of FST for the whole population was from 0.041 (6L3F8) -0.195 (Sat8). The mean FST was 0.099±0.010; the average of total inbreeding coeffient (FIT) was -0.004±0.052; the mean inbreeding coeffient among populations (FIS) was -0.114±0.050. The dendrogram by Unweighed Pair-Group Method with Arithmetic averaging (UPGMA) based on Nei’s genetic distance and Reynolds' genetic distance was similar. Seven populations were clustered into 4 groups. The Germany Angora Rabbit, American Rex Rabbit and Wan-line Angora Rabbit belonged to the first group; the New Zealand White Rabbit and Zika Rabbit were included in the second group; the Fujian Yellow Rabbit and Fujian Black Rabbit were clustered separately. The results suggested that the 15 microsatellite loci were effective markers for analysis of genetic relationships among rabbit populations.
  Wu Tian-Wen , Li-Ting , Feng-Kai , Mao-Liu-Liu , Li Bi-Chun and Wu Xin-Sheng
  Researchers had scanned the complete CDS of rabbit KAP3.1 and KAP6.1 gene for the 1st time by PCR-SSCP and detected that their homology with mouse, chimpanzee, human, sheep and goat were >80%, suggesting that the two genes among different species kept high conservation in evolution. In this study, three genotypes and two alleles were found at KAP3.1-1 locus having one silent mutation (C91T) and there were two alleles and three genotypes in 5 un-translated region of KAP6.1 gene with the A 755 G mutation of the enhancer region. Herein, the distribution of these genotypes in all populations was consistent with the Hardy-Weinberg equilibrium and showed intermediate or low diversity. The result suggested a theoretical reference to the breeding of rabbit wool quality whether KAP3.1 and KAP6.1 gene could be a molecular marker or not.
  Wu Xin-Sheng , Wu Tian-Wen , Zhao Hui-Ling , Cheng Guang-Long , Xu Qi , Cheng Jin-Hua , Zhu Xiu-Bai and Chen Guo-Hong
  Correlation analysis of wool yield at age of one year in Wan line Angora rabbits using microsatellite DNA markers was studied. The results showed that the average number of alleles per locus was 4.5 (from 3 to 6) in Wan line Angora rabbits. The mean heterozygosity and the average polymorphism information content were 0.680 (from 0.630 to 0.721) and 0.642 (from 0.559 to 0.705), respectively. The relationships were not significant between Sat4, Sat13, So144 loci and wool yield (p>0.05). The relationship was significant between Sol33 locus and wool yield (p<0.05) and wool yield of genotype AD and BD were significantly higher than that of the other three genotypes which did not have gene D (p<0.05). This may indicated that the gene D could synergize wool yield of Wan line Angora rabbits.
 
 
 
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