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Articles by Weiping Yin
Total Records ( 2 ) for Weiping Yin
  Jiayu Gao , Xiao Yang , Pu Liu and Weiping Yin
  Background and Objective: The polysaccharides of herbal Scutellaria barbata (PSB) are a group of active components with multiple bioactivities. Though PSB is thought to constitute the main medicinal properties of Scutellaria barbata (S. barbata) the current study on the isolation of PBS and their anticancer and antioxidant activities are rare and thus, remain as an ongoing challenge. Therefore, the purpose of this study is to further develop a new isolation technique for industrial production of PSB and also determine the anticancer and antioxidant properties of PBS in vitro. Methodology: This study optimized an ultrasonic-assisted method for PSB isolation and led to the yield rate at 2.96±0.24%. The isolated PSB were able to induce the apoptosis of the human esophageal cancer EC109 cell line and human liver cancer HepG2 cell line in Annexin-V staining apoptotic assay. Results: The IC50 of PSB on EC109 and HepG2 were determined as 43.62±1.51 μg mL–1 and 60.44±2.31 μg mL–1, respectively using MTT cytotoxic test. Moreover, PSB demonstrated the dose-dependent antioxidant and free radical scavenging activities in the total antioxidant activity and ABTS free radical scavenging assay. Its highest effect was comparable to that of the positive control butylated hydroxytoluene (BHT). Conclusion: In this study, the method of PSB isolation is significantly developed and characterized with higher yield rate and less time/resources consuming which greatly facilitate the current and future study of PSB. Meanwhile, the growth inhibitory and apoptotic effects of PSB are firstly determine in esophageal cancer cell and human liver cancer cell in vitro. The PSB is confirmed as an active anticancer and antioxidant component of S. barbata providing a potential natural medicinal resource for further investigation and development. Moreover, the results of this study underpin the fundamental understanding of the medicinal activities of S. barbata and support its current use in clinic of China.
  Jiayu Gao , Xiao Yang , Jiangxia Hu and Weiping Yin
  Background and Objective: Gallnuts have been traditionally used as a source of drugs for the treatment of cancerous diseases in folk medicinal system around world. To reveal the anticancer phytochemical foundation of Gallnuts, the secondary metabolite maps were constructed to correlate the chemistry with the cytotoxicity of crude extracts on human cancer cells. Materials and Methods: The growth inhibition activities were determined by MTT assay of 8 solvent extracts of gallnuts Galla Chinensis and Galla Turcica in the human liver cancer cell line 7721. The activities were then mapped onto the secondary metabolite profile of crude extracts by principal components analysis (PCA) of HPLC-UV data. The top five PCA components of the map discriminated extract activity mainly based on the differential content of five compounds, which two of them were then tested individually using MMT assay on cancer cells in vitro. Results: PCA-constructed secondary metabolite mapping quickly identified functional compounds from crude gallnuts extracts. Galla Chinensis and Galla Turcica constituents gallic acid and 1, 2, 3, 4, 6-O-pentagalloyl glucose inhibited the growth of human liver cancer cell line growth with activities (IC50: 36.44±8.75 and 55.59±14.96 μg mL–1, respectively) comparable to that of cisplatin (IC50: 35.12±1.74 μg mL–1). Conclusion: Gallic acid and 1, 2, 3, 4, 6-O-pentagalloyl glucose are identified as the anticancer constituents of Gallnuts. It thus strongly underpins a fundamental understanding of anticancer activities of gallnuts medicine and supports their ongoing clinical uses in China. Moreover, this work may confirm the potential of HPLC-PCA analysis as a tool for quick identification of functional compounds of plant extracts.
 
 
 
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