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Articles by Wamidh H. Talib
Total Records ( 2 ) for Wamidh H. Talib
  Reem A. Issa, , Wamidh H. Talib and Maha Habash
  Background: This study was undertaken to evaluate the phytochemical composition of the aqueous extract obtained from Jordanian premature Psidium guajava fruit peel (PGFP) and their antimicrobial effect, using two different methods for extraction. Materials and Methods: Two different methods for extraction were used: Microwave Integrated Soxhlet (MIS) extraction and Conventional Soxhlet (CS) extraction. The TLC analysis was used to optimize each extraction method and for the preliminary phytochemical screening. The HPLC-MS/MS was used for the identification and the quantification of the phenol compounds in the plant. The antimicrobial activity of both aqueous PGFP extracts was measured using micro-titer plate dilution method. Results: Four phenol compounds were identified in the MIS extract, of which ascorbic acid showed the major constituent, while six phenol compounds were identified in the CS extract, of which ellagic acid was the major constituent. Moreover, the MIS extract contained higher levels of total phenol compounds compared to the CS extract. According to the antimicrobial assay, the two extracts of PGFP showed inhibitory activity against both Gram-positive and Gram-negative bacteria. The Minimum Inhibitory Concentration (MIC) of MIS extract against Escherichia coli, Staphylococcus aureus, Propioni bacterium acnes and Bacillus cereus was twice less than the values obtained from the CS extract, except for S. aureus, where the two extracts were equally active. Conclusion: Optimization of the extraction method used is required in order to increase the content of the polar portion in the extract, which showed to have the highest antimicrobial activity. These data can be utilized in developing combined remedies composed of synthetic antibiotics with phytochemicals obtained from P. guajava peel extracts, which can be used for different purposes; such as functional food development and in food preservative.
  Wamidh H. TALIB and Adel M. MAHASNEH
  Forty four extracts from sixteen plants used traditionally as anticancer agents were evaluated in vitro for their antiproliferative activity against Hep-2, MCF-7, and Vero cell lines. Plants were fractionated using ethanol, methanol, chloroform, n-hexane, distilled water, and butanol. The antiproliferative activity was measured by MTT assay. TLC was used to identify active fractions. The apoptotic activity of active fractions was determined using TUNEL colorimetric assay. 20 of these extracts demonstrated significant antiproliferative activity against one or more of the cell lines. These extracts were prepared from Ononis hirta, Inula viscosa, Salvia pinardi, Verbascum sinaiticum and Ononis sicula. Methanol fractions of Ononis hirta (aerial parts) and Inula viscosa (flowers) were the most active fractions against MCF-7 cells with IC50 of 27.96 and 15.78 μg/ml respectively and they were less toxic against other cell lines. Other extracts showed lower activity against cancer cell lines. TLC analysis showed the presence of flavonoids and terpenoids in active plants while alkaloids were detected in Ononis hirta (aerial parts) extracts. Ononis hirta (aerial parts) and Inula viscosa (flowers) extracts exerted their antiproliferative activity by inducing apoptosis in cancer cell lines. Further studies are necessary for detailed chemical characterization and more extensive biological evaluation of the most active ingredients.
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