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Articles by Wafaa Tawfik Abbas
Total Records ( 2 ) for Wafaa Tawfik Abbas
  Mamdouh Yousif Elgendy , Iman Kamel Abumourad , Shimaa Elsayed Mohamed Ali , Waleed Salah El-Din Soliman , Taghreed Borhan El-Din Ibrahim and Wafaa Tawfik Abbas
  Background and Objective: Fish health and aquatic environment are strongly interrelated. Aquatic environment receive array of anthropogenically derived chemicals with deleterious health effects on the cohabitant aquatic animals and threatens their lives. The aim of this study was to evaluate the health status of Tilapia zillii and Solea vulgaris captured from Lake Qarun, Egypt known to receive lofty loads of contaminants as well as to monitor the genotoxic effects of some heavy metals detected in lake water on the two fish species. Methodology: Heavy metals (Cu, Zn, Cd, Ni and Pb) were assessed in water and fish specimens (Tilapia zillii and Solea vulgaris) collected from Lake Qarun and the mean value was recorded. Fish and water samples were subjected to microbiological and parasitological examination. Additionally, genotoxicity was analysed using comet assay. Results: Heavy metals; Zn, Ni and Cu showed high values in water exceeding the recommended limits. Accumulation of heavy metals was noticed in fish muscles and Ni was the uppermost detected metal. Genotoxicity was confirmed in fish liver cells by comet assay. The DNA damage was quantified as; tail length, DNA percentage in tail, tail moment and number of cells with tail. Genotoxicity was severe in Solea vulgaris than Tilapia zillii. Bacterial infections; Vibrio alginolyticus (21 isolate), Aeromonas hydrophila (13), Photobacterium damselae subsp. piscicida (7) were detected in fish specimens. Parasitic Isopoda sp. were found attached to the ventral body surface and inside the gill chamber of fish. Conclusion: The results of this study indicate that Lake Qarun is contaminated with substances that are genotoxic to fish as well as predispose fish to numerous opportunistic pathogens.
  Sayeda Mohammed Ali , Hoda Shafeek Nasr and Wafaa Tawfik Abbas
  Treatment of aquarium wastewater represents an important process to clean and recycle wastewater to be safely returned to the environment, used for cultivation or to minimize the multiple renewal of water. Chlorella vulgaris was an important freshwater microalgae which used in wastewater treatment, and increasing its potential of treatment can be achieved with existence of N2-fixing bacteria. Co-culturing of Chlorella vulgaris with the diazotrophs, Azospirillum brasilense or Azotobacter chroococcum in three different media; aquarium wastewater (AWW), sterile enriched natural aquarium wastewater (GPM) and synthetic wastewater media (SWW) were studied. Biomass yield of the microalgae was estimated by determination of chlorophylls (a and b), total carotenoid and the dry weight of C. vulgaris. Also determination of ammonia, nitrite, phosphate and nitrate in the culture were done. The presence of diazotrophs significantly increased the biomass of C. vulgaris by increasing its microalgae pigments (chlorophylls a and b, and total carotenoids). The highest pigments percentage was reported due to addition of A. brasilense to C. vulgaris (18.3-133.5%) compared to A. chroococcum (23.9-56.9%). As well as increased dry weight from 12 to 50%. There was also improved removal of nitrate, nitrite, ammonia and phosphate; where, the highest removal percentage was reported due to addition of A. chroococcum to C. vulgaris (0.0-52%) compared to A. brasilense (0.6-16.4%). A. brasilense and A. chroococcum can support C. vulgaris biomass production and bioremediation activity in the aquarium to minimize the periodical water renewal.
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